Gene Dmel\tsu

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

In direction of increasing cytology: Pmm45A- tsu- Mys45A-

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\tsu for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

tsu-RA

FBtr0088567

NM_136610

693

165

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

tsu-PA

FBpp0087648

19.0

165

4.92

NP_610454

AAF58987

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

PANTHER - Protein classification by function, families, and pathways

•

FBgn0033378

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

RNA recognition motif, RNP-1 (IPR000504)

RNA binding motif protein 8 (IPR008111)

Nucleotide-binding, alpha-beta plait (IPR012677)

PDB - Protein Data Bank. An information portal to biological macromolecular structures

•

1HL6

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9V535

UniProtKB/TrEMBL

Maps to

DS02099

(BDGP Project Members, 2000-)

Does NOT map to

Identified with

RE09075

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

rescue fragment

tsu[+tMa]
2R:4,996,786..5,001,165

comment=position of restriction fragment on reference sequence inferred by FlyBase curator
evidence=experimental
linked_to=NheI-EagI_rfrag

sequence variant

tsu[2]
2R:4,998,932..4,998,933

comment=G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation (exact site of mutation unspecified).
evidence=experimental
pr_change=W148@|tsu-PA
reported_pr_change=W148@

External Data

(Gatfield and Izaurralde, 2002)

DEDB - Drosophila exon database: splicing graphs

•

13252

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

(Christensen and Cook, 2007.3.22)

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0033378

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0033378

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0033378

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

viable (with tsu⁷)

viable (with tsu⁶)

viable (with tsu⁵)

lethal (with Df(2R)Np5)

lethal | recessive

lethal | recessive, with Scer\GAL4[-]:

tsu^EP567

lethal | embryonic stage | germ-line clone | recessive, with Scer\GAL4[-]:

tsu^EP567

cell lethal | partially

tsu^dsRNA.cGa

Other Phenotypes

Allele

cell growth defective

tsu^dsRNA.cLa

Sterility

Allele

female sterile (with tsu⁷)

female sterile (with tsu⁶)

female sterile (with tsu⁵)

female sterile | recessive

Phenotype manifest in

Allele

egg (with tsu⁷)

tsu⁶

dorsal appendage (with tsu⁷)

tsu⁶

egg chamber (with tsu⁷)

tsu⁶

egg (with tsu⁶)

tsu⁷

dorsal appendage (with tsu⁶)

tsu⁷

egg chamber (with tsu⁶)

tsu⁷

egg

tsu⁷

dorsal appendage

tsu⁷

oocyte nucleus

tsu⁷

egg | germ-line clone, with Scer\GAL4[-]:

tsu^EP567

Classical Alleles ( 8 )

For All Classical Alleles Show

Allele of tsu	Class	Mutagen	Stocks	Known lesion
tsu²		ethyl methanesulfonate	0	Yes
tsu³		P-element activity	0	Yes
tsu⁴		P-element activity	0	Yes
tsu⁵		P-element activity	0	Yes
tsu⁶	hypomorph	P-element activity	0	Yes
tsu⁷	hypomorph	P-element activity	0	Yes
tsu^EP567		P-element activity	2	Yes
tsu^KG04415			0	--

Alleles Carried on Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Allele of tsu	Mutagen	Stocks	Known lesion
tsu^+tMa	in vitro construct \| genomic fragment	0	Yes
tsu^GD14025	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
tsu^dsRNA.cGa	in vitro construct \| RNAi	0	Yes
tsu^dsRNA.cLa	in vitro construct \| RNAi	0	--

Aneuploid Aberrations

Useful deficiency

Df(2R)w45-30n

Disrupted in

Df(2R)BSC271

Df(2R)Np3

(Mohr et al., 2001, Hachet and Ephrussi, 2001, Mohr and Boswell, 2002)

Df(2R)Np4

(Hachet and Ephrussi, 2001)

Df(2R)Np5

(Mohr et al., 2001, Hachet and Ephrussi, 2001, Mohr and Boswell, 2002)

Df(2R)w45-30n

Not disrupted in

Df(2R)44CE

Df(2R)G75

Df(2R)H3E1

Df(2R)w73-2

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD14025}

characterization construct

P{tsu^+tMa}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{?EP}tsu⁵

P{SUPor-P}tsu^KG04415

insertion of mobile activating element

P{EP}tsu^EP567

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 13 )

Molecular Function

CV term

Evidence

References

mRNA binding

inferred from sequence or structural similarity

(Lasko, 2000)

inferred from sequence or structural similarity with HGNC_gene:RBM8A; HGNC:9905; OMIM:605313

(FlyBase Curators et al., 2004-)

non-traceable author statement

(Mohr, 1999.7.30)

nucleotide binding

inferred from electronic annotation with InterPro:IPR012677

Biological Process

CV term

Evidence

References

RNA processing

inferred from electronic annotation with InterPro:IPR008111

(FlyBase Curators et al., 2004-)

establishment of pole plasm mRNA localization

traceable author statement

(Farina and Singer, 2002)

mRNA export from nucleus

non-traceable author statement

microtubule cytoskeleton organization and biogenesis

inferred from mutant phenotype

oocyte anterior/posterior axis determination

inferred from mutant phenotype

oocyte dorsal/ventral axis determination

inferred from mutant phenotype

oocyte nucleus migration during oocyte axis determination

inferred from mutant phenotype

pole plasm oskar mRNA localization

traceable author statement

(Tekotte and Davis, 2002)

traceable author statement

(Farina and Singer, 2002)

regulation of pole plasm oskar mRNA localization

inferred from mutant phenotype AND inferred from physical interaction with FLYBASE:mago; FB:FBgn0002736

Cellular Component

CV term

Evidence

References

cytoplasm

inferred from direct assay

nucleus

inferred from direct assay

inferred from direct assay

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

tsu allele

Gene

References

External Data

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

61762

Orthologs

Genome-wide drosophilid orthologs

Dana\GF11955

Dere\GG23412

Dgri\GH19854

Dmoj\GI19803

Dper\GL17620

Dpse\GA21317

Dsec\GM21093

Dsim\GD10630

Dvir\GJ18464

Dwil\GK23248

Dyak\GE19253

Curated drosophilid orthologs

Dpse\GA21317

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000013045
Anopheles gambiae (Ensembl)
ENSBTAP00000011016
Bos taurus (Ensembl)
ENSCINP00000010079
Ciona intestinalis (Ensembl)
NEWSINFRUP00000138758
Takifugu rubripes (Ensembl)
ENSP00000254105
Homo sapiens (Ensembl)
ENSMMUP00000021116
Macaca mulatta (Ensembl)
ENSMODP00000023377
Monodelphis domestica (Ensembl)
ENSPTRP00000041292
Pan troglodytes (Ensembl)
ENSRNOP00000028807
Rattus norvegicus (Ensembl)
GSTENP00013208001
Tetraodon nigroviridis (Ensembl)
Q688D4
Oryza sativa (Gramene)
Q75KH5
Oryza sativa (Gramene)
AT1G51510.1
Arabidopsis thaliana (Arabidopsis)
WBGene00037565
Caenorhabditis briggsae (Wormbase)
WBGene00004387
Caenorhabditis elegans (Wormbase)
ZDB-CDNA-050306-51
Danio rerio (Zfin)
ZDB-GENE-050306-51
Danio rerio (Zfin)
DDB0218780
Dictyostelium discoideum (Dictybase)
MGI:1913129
Mus musculus (Informatics)
MGI:3612447
Mus musculus (Informatics)
SPAC23A1.09
Schizosaccharomyces pombe (Sanger)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 5 )

Szeged

P{EP}tsu^EP567

VDRC

v36024

w¹¹¹⁸; P{GD14025}v36024

v36025

w¹¹¹⁸; P{GD14025}v36025

Bloomington

14097

y¹; P{SUPor-P}KG04415/CyO; ry⁵⁰⁶

6597

w¹¹¹⁸; tsu^EP567 bw¹ sp¹/SM5

Genomic Clones ( 2 )

cDNA Clones ( 10 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

RE09075

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0033378

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0033378

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for identity of: tsu CG8781 Source for identity of tsu CG8781 was sequence comparison (date:011219).

(FlyBase, 1996-)

Source for database merge of

Additional comments

Comments About Role

tsu is required in the germline for the localisation of germ cell determinants and axis formation during oogenesis.

mago and tsu are required for cell viability.

Comments About Molecular Function

The mago/tsu physical interaction is conserved, occurs in vitro and in vivo and is not RNA mediated. mago and tsu gene products localize within nuclear speckles. mago/tsu heterodimers associate with spliced mRNAs carrying an exon-exon junction complex.

Other Comments

Identification: in a yeast two hybrid screen for proteins that bind mago protein. Etymology: gene is named "tsunagi", which is Japanese for "connection" or "link".