Gene Dmel\cul-4

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General Information

Symbol

Dmel\cul-4

Species

D. melanogaster

Name

cul-4

Annotation symbol

CG8711

Feature type

protein_coding_gene

FlyBase ID

FBgn0033260

Created / Updated

2003-12-02/2003-12-02

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

44A4-44A4

Sequence location

2R:3,989,763..3,993,814 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol cul-4 (CG8711, FBgn0033260). It has the cytological map location 44A4. Its sequence location is 2R:3989763..3993814. Its molecular function is described as: ubiquitin-protein ligase activity; ubiquitin protein ligase binding. It is involved in the biological processes: positive regulation of NFAT protein import into nucleus; ubiquitin-dependent protein catabolic process. 4 alleles are reported. The phenotype of these alleles is annotated with eye. It has one annotated transcript and one annotated polypeptide.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

44A4-44A4

Limits computationally determined from genome sequence between P{lacW}Vps28^k16503 and P{EP}kermit^EP493

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\cul-4 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

cul-4-RA

FBtr0088821

NM_136508

3339

821

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

cul-4-PA

FBpp0087897

94.4

821

8.98

NP_610352

AAF59135

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0033260

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Cullin, N-terminal region (IPR001373)

Winged helix repressor DNA-binding (IPR011991)

Cullin, conserved site (IPR016157)

Cullin homology (IPR016158)

Cullin repeat-like (IPR016159)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

13387

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

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FBgn0033260

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

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FBgn0033260

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

cell lethal | somatic clone

cul-4^unspecified

Phenotype manifest in

Allele

eye | somatic clone

cul-4^unspecified

Classical Alleles ( 3 )

For All Classical Alleles Show

Allele of cul-4	Mutagen	Stocks	Known lesion
cul-4^EP2518	P-element activity	2	--
cul-4^KG02900	P-element activity	1	--
cul-4^unspecified		0	--

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of cul-4	Class	Mutagen	Stocks	Known lesion
cul-4^GD14006		in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes

Aneuploid Aberrations

Useful deficiency

Df(2R)CA53

Useful duplication

Dp(2;2)Y3b

Disrupted in

Df(2R)BSC267

(Christensen et al., 2007.10.29)

Df(2R)Exel6056

(Ryder, 2004.4.26)

Df(2R)Exel7094

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD14006}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{SUPor-P}cul-4^KG02900

insertion of mobile activating element

P{EP}cul-4^EP2518

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 6 )

Molecular Function

CV term

Evidence

References

ubiquitin protein ligase binding

inferred from electronic annotation with InterPro:IPR001373, InterPro:IPR016157, InterPro:IPR016158

(FlyBase Curators et al., 2004-)

ubiquitin-protein ligase activity

inferred from sequence or structural similarity with SGD_LOCUS:CDC53; SGD:S0002290

(FlyBase, 1992-)

Biological Process

CV term

Evidence

References

positive regulation of NFAT protein import into nucleus

inferred from mutant phenotype

(Gwack et al., 2007)

ubiquitin-dependent protein catabolic process

inferred from electronic annotation with InterPro:IPR001373, InterPro:IPR016157, InterPro:IPR016158

(FlyBase Curators et al., 2004-)

Cellular Component

CV term

Evidence

References

cullin-RING ubiquitin ligase complex

inferred from electronic annotation with InterPro:IPR001373, InterPro:IPR016157, InterPro:IPR016158

(FlyBase Curators et al., 2004-)

nuclear ubiquitin ligase complex

inferred from sequence or structural similarity with SGD_LOCUS:CDC53; SGD:S0002290

(FlyBase, 1992-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

cul-4 allele

Gene

References

unspecified

(Mueller et al., 2005)

(Singh et al., 2005)

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

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61637

Orthologs

Genome-wide drosophilid orthologs

Dana\GF11213

Dere\GG10678

Dgri\GH19988

Dmoj\GI20275

Dper\GL17383

Dpse\GA21273

Dsec\GM20725

Dvir\GJ20228

Dwil\GK23161

Dyak\GE23323

Curated drosophilid orthologs

Dpse\GA21273

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000021534
Anopheles gambiae (Ensembl)
ENSBTAP00000026769
Bos taurus (Ensembl)
ENSCAFP00000027364
Canis familiaris (Ensembl)
ENSCINP00000002400
Ciona intestinalis (Ensembl)
NEWSINFRUP00000133212
Takifugu rubripes (Ensembl)
ENSGALP00000013926
Gallus gallus (Ensembl)
ENSP00000353722
Homo sapiens (Ensembl)
ENSMMUP00000011845
Macaca mulatta (Ensembl)
ENSMODP00000012963
Monodelphis domestica (Ensembl)
ENSPTRP00000010296
Pan troglodytes (Ensembl)
ENSRNOP00000003523
Rattus norvegicus (Ensembl)
GSTENP00017943001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000009705
Xenopus tropicalis (Ensembl)
Q6F3B6
Oryza sativa (Gramene)
AT5G46210.1
Arabidopsis thaliana (Arabidopsis)
ZDB-CDNA-040425-2291
Danio rerio (Zfin)
ZDB-GENE-040426-1357
Danio rerio (Zfin)
DDB0191643
Dictyostelium discoideum (Dictybase)
MGI:1919834
Mus musculus (Informatics)
SPAC3A11.08
Schizosaccharomyces pombe (Sanger)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 4 )

Szeged

P{EP}cul-4^EP2518

VDRC

v44829

w¹¹¹⁸; P{GD14006}v44829

Bloomington

17253

w¹¹¹⁸; P{EP}cul-4^EP2518

13335

y¹; P{SUPor-P}cul-4^KG02900/CyO; ry⁵⁰⁶

Genomic Clones ( 2 )

cDNA Clones ( 42 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0033260

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0033260

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Comments About Role

Comments About Molecular Function

cul-4 blocks degradation of dup protein after γ-irradiation.

(Higa, 2003)

Other Comments

S2 cells are treated with dsRNA made from templates generated with primers directed against this gene to assess effect on degradation of dup and geminin genes.

(Higa, 2003)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•