Gene Dmel\CG12736

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General Information

Symbol

Dmel\CG12736

Species

D. melanogaster

Name

CG12736

Annotation symbol

CG12736

Feature type

protein_coding_gene

FlyBase ID

FBgn0033184

Created / Updated

2003-12-02/2003-12-02

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

43D1-43D1

Sequence location

2R:3,396,032..3,397,959 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol CG12736 (FBgn0033184). It has the cytological map location 43D1. Its sequence location is 2R:3396032..3397959. Its molecular function is described as: GTPase activity; translation elongation factor activity; GTP binding. It is involved in the biological processes: translation; translational elongation. 3 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

43D1-43D1

Limits computationally determined from genome sequence between P{lacW}cos^k16101 and P{EP}CG1600^EP398

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\CG12736 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

CG12736-RA

FBtr0088981

NM_136444

1808

456

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

CG12736-PA

FBpp0088054

49.7

456

7.78

NP_610288

AAF59240

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0033184

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Protein synthesis factor, GTP-binding (IPR000795)

Translation elongation factor EFTu/EF1A, C-terminal (IPR004160)

Translation elongation factor EFTu/EF1A, domain 2 (IPR004161)

Translation elongation factor EFTu/EF1A, bacterial and organelle (IPR004541)

Translation elongation and initiation factors/Ribosomal, beta-barrel (IPR009000)

Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal (IPR009001)

Small GTP-binding protein (IPR005225)

MitoDrome - A database of annotated Dmel nuclear genes encoding mitochondrial proteins

•

MTDROME12736

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q7K3V6

Maps to

Does NOT map to

Identified with

(BDGP Project Members, 2000-)

(Oliver, 2001.8.16)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

13524

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI113808

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0033184

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0033184

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0033184

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

CG12736^f00932

Sterility

Allele

fertile

CG12736^f00932

Phenotype manifest in

Allele

Classical Alleles ( 2 )

For All Classical Alleles Show

Allele of CG12736	Class	Mutagen	Stocks	Known lesion
CG12736^HP25926			1	--
CG12736^f00932		piggyBac transposase	2	--

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of CG12736	Class	Mutagen	Stocks	Known lesion
CG12736^GD7815		in vitro construct \| RNAi in vitro construct \| regulatory fusion	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(2R)Drl^rv22

Disrupted in

Df(2R)Exel6052

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD7815}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{WH}CG12736^f00932

insertion of mobile activating element

P{EPg}CG12736^HP25926

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 6 )

Molecular Function

CV term

Evidence

References

GTP binding

inferred from electronic annotation with InterPro:IPR000795, InterPro:IPR004160, InterPro:IPR004161, InterPro:IPR004541, InterPro:IPR005225

(FlyBase Curators et al., 2004-)

GTPase activity

inferred from sequence or structural similarity with UniProtKB:P49410

(FlyBase, 1992-)

translation elongation factor activity

inferred from sequence or structural similarity with UniProtKB:P49410

(FlyBase, 1992-)

inferred from sequence or structural similarity with UniProtKB:P49411

(Sardiello et al., 2003.6.11)

Biological Process

CV term

Evidence

References

translation

inferred from sequence or structural similarity with UniProtKB:P49410

(FlyBase, 1992-)

translational elongation

inferred from sequence or structural similarity with UniProtKB:P49411

(Sardiello et al., 2003.6.11)

Cellular Component

CV term

Evidence

References

mitochondrion

inferred from sequence or structural similarity with UniProtKB:P49410

(FlyBase, 1992-)

inferred from sequence or structural similarity with UniProtKB:P49411

(Sardiello et al., 2003.6.11)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

CG12736 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

61557

Orthologs

Genome-wide drosophilid orthologs

Dana\GF13674

Dere\GG10739

Dgri\GH19814

Dmoj\GI20403

Dper\GL20115

Dpse\GA11779

Dsec\GM20785

Dsim\GD15306

Dvir\GJ20075

Dwil\GK19666

Dyak\GE23915

Curated drosophilid orthologs

Dpse\GA11779

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000010016
Anopheles gambiae (Ensembl)
WBGene00033168
Caenorhabditis briggsae (Wormbase)
WBGene00007001
Caenorhabditis elegans (Wormbase)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

Harvard

PBac{WH}CG12736^f00932

Bloomington

21943

w¹¹¹⁸; P{EPg}CG12736^HP25926

18393

w¹¹¹⁸; PBac{WH}CG12736^f00932

Genomic Clones ( 2 )

cDNA Clones ( 98 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0033184

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0033184

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments