Gene Dmel\Atf6

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General Information

Symbol

Dmel\Atf6

Species

D. melanogaster

Name

Atf6

Annotation symbol

CG3136

Feature type

protein_coding_gene

FlyBase ID

FBgn0033010

Created / Updated

2006-06-12/2007-06-14

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

41D4-41E1

Sequence location

2R:1,032,202..1,042,266 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol Atf6 (CG3136, FBgn0033010). It has the cytological map location 41D4-41E1. Its sequence location is 2R:1032202..1042266. Its molecular function is described as: protein homodimerization activity; sequence-specific DNA binding; protein dimerization activity; transcription factor activity. It is involved in the biological process regulation of transcription, DNA-dependent. 3 alleles are reported. No phenotypic data is available. It has 3 annotated transcripts and 3 annotated polypeptides.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

41D4-41E1

Limits computationally determined from genome sequence between P{PZ}vlc⁰⁷⁰²²&P{lacW}l(2)09851^k08138 and P{PZ}l(2)09851⁰⁹⁸⁵¹&P{lacW}Src42A^k10108

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

In direction of increasing cytology: RpL38- Stlk+ p120ctn+ P{SUPor-P}KG01086+ CG17486+ CG17883+ Nipped-B+ CR41440+ P{SUPor-P}CG40282<up>KG08252</up>+ CG17082+ CG12547+ CG17528+ CG14464+ TpnC41C+ CG3107+ P{SUPor-P}KG03359+ gus- P{SUPor-P}KG05308+ CG3136+ Nipped-A- P{SUPor-P}KG10162- d4+ P{SUPor-P}KG01463- d4+ Ogt+ CG10465- CG10395+ CG30441- CG10396+ CG10417- CG30437+ CG32838+ CG30440- CG30438+ P{SUPor-P}KG02589- TpnC4- P{SUPor-P}KG06088- CG30438+ CG17510- CG17508+ P{SUPor-P}KG02079- P{SUPor-P}KG00632+ CG11665- CG1344+ l(2)NC136- CG8245+ CG1298+ scarface- P{EPgy2}EY07370- CG17337+ vlc+ ap-

(Myster et al., 2004)

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Atf6 for information on other features

Comments on Gene Model

Transposon inserted in intron

DGC clone GH05757 appears problematic: initiates within transposon sequences in the first intron.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Atf6-RA

FBtr0086079

NM_165423

3117

739

Atf6-RB

FBtr0086080

NM_136315

3964

739

Atf6-RC

FBtr0086081

NM_206023

2518

741

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Atf6-PA

FBpp0085415

84.6

739

9.70

NP_724403

AAF57345

Atf6-PB

FBpp0085416

84.6

739

9.70

NP_610159

AAM68375

Atf6-PC

FBpp0085417

84.9

741

9.68

NP_995745

AAS64774

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0033010

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Basic-leucine zipper (bZIP) transcription factor (IPR004827)

bZIP transcription factor, bZIP-1 (IPR011616)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

11637

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI109723
AI107880

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0033010

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0033010

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0033010

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for CG3136

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for Atf6

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

Atf6^c05057

Sterility

Allele

fertile

Atf6^c05057

Phenotype manifest in

Allele

Classical Alleles ( 2 )

For All Classical Alleles Show

Allele of Atf6	Class	Mutagen	Stocks	Known lesion
Atf6^F33.42		spontaneous	0	Yes
Atf6^c05057		piggyBac transposase	2	--

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Atf6	Class	Mutagen	Stocks	Known lesion
Atf6^GD14782		in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes

Aneuploid Aberrations

Useful deficiency

Df(2R)nap14

Useful duplication

Dp(2;2)BG

Disrupted in

Df(2R)Exel9021

(Ryder, 2004.4.26)

Df(2R)M41A10

(Myster et al., 2004)

Df(2R)M41A8

(Myster et al., 2004)

Df(2R)Nipped-D

(Myster et al., 2004)

Not disrupted in

Df(2R)M41A2

(Myster et al., 2004)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD14782}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{PB}Atf6^c05057

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 6 )

Molecular Function

CV term

Evidence

References

protein dimerization activity

inferred from electronic annotation with InterPro:IPR004827, InterPro:IPR011616

(FlyBase Curators et al., 2004-)

protein homodimerization activity

inferred from sequence or structural similarity

(Fassler et al., 2002)

sequence-specific DNA binding

inferred from electronic annotation with InterPro:IPR004827, InterPro:IPR011616

(FlyBase Curators et al., 2004-)

transcription factor activity

inferred from electronic annotation with InterPro:IPR004827, InterPro:IPR011616

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

regulation of transcription, DNA-dependent

inferred from electronic annotation with InterPro:IPR004827, InterPro:IPR011616

(FlyBase Curators et al., 2004-)

Cellular Component

CV term

Evidence

References

nucleus

inferred from electronic annotation with InterPro:IPR004827, InterPro:IPR011616

(FlyBase Curators et al., 2004-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Atf6 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

61397

Orthologs

Genome-wide drosophilid orthologs

Dere\GG23145

Dgri\GH21728

Dmoj\GI20675

Dper\GL22941

Dpse\GA16207

Dsec\GM26735

Dsim\GD17476

Dvir\GJ20425

Dwil\GK21942

Dyak\GE11353

Curated drosophilid orthologs

Dpse\GA16207

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000026313
Anopheles gambiae (Ensembl)
ENSCAFP00000019250
Canis familiaris (Ensembl)
ENSGALP00000004179
Gallus gallus (Ensembl)
ENSP00000263291
Homo sapiens (Ensembl)
ENSMODP00000001169
Monodelphis domestica (Ensembl)
ENSPTRP00000002668
Pan troglodytes (Ensembl)
ENSRNOP00000032766
Rattus norvegicus (Ensembl)
GSTENP00020188001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000011236
Xenopus tropicalis (Ensembl)
WBGene00023594
Caenorhabditis briggsae (Wormbase)
ZDB-GENE-041014-328
Danio rerio (Zfin)
MGI:105121
Mus musculus (Informatics)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

Harvard

PBac{PB}Atf6^c05057

VDRC

v36504

w¹¹¹⁸; P{GD14782}v36504

Bloomington

17689

w¹¹¹⁸; PBac{PB}Atf6^c05057

Genomic Clones ( 2 )

cDNA Clones ( 26 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0033010

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0033010

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for identity of: Atf6 CG3136

(Hollien and Weissman, 2006)

Source for database merge of

Additional comments

Comments About Role

Comments About Molecular Function

Other Comments

Changes in expression profiles associated with the unfolded protein response have been analyzed in S2 cells following treatment with dsRNA made from templates generated with primers directed against this gene.

(Hollien and Weissman, 2006)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•