Gene Dmel\SP1070

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General Information

Symbol

Dmel\SP1070

Species

D. melanogaster

Name

SP1070

Annotation symbol

CG9138

Feature type

protein_coding_gene

FlyBase ID

FBgn0031879

Created / Updated

2003-12-01/2008-02-05

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

27D2-27D2

Sequence location

2L:6,972,828..7,009,888 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol SP1070 (CG9138, FBgn0031879). It has the cytological map location 27D2. Its sequence location is 2L:6972828..7009888. Its molecular function is described as: Notch binding; receptor activity; calcium ion binding. It is involved in the biological process cell adhesion. 3 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

27D2-27D2

Limits computationally determined from genome sequence between P{PZ}snRNP70K⁰²¹⁰⁷ and P{PZ}wg⁰²⁶⁵⁷

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

27D1-27D2

(Serano, 2000.2.28)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\SP1070 for information on other features

Comments on Gene Model

DGC clone RH55424 appears problematic: contains intronic and intergenic sequence

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

SP1070-RA

FBtr0079354

NM_135247

11701

3561

SP1070-RB

FBtr0290119

11636

3557

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

SP1070-PA

FBpp0078982

387.5

3561

4.96

NP_609091

AAF52472

SP1070-PB

FBpp0288558

387.1

3557

4.93

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0031879

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Aspartic acid and asparagine hydroxylation site (IPR000152)

Coagulation factor 5/8 type, C-terminal (IPR000421)

Sushi/SCR/CCP (IPR000436)

EGF-like, type 3 (IPR000742)

CUB (IPR000859)

EGF-like, type 2 (IPR001438)

EGF-like calcium-binding (IPR001881)

Low density lipoprotein-receptor, class A, cysteine-rich (IPR002172)

Hyalin (IPR003410)

EGF-like (IPR006209)

EGF (IPR006210)

Galactose-binding like (IPR008979)

Concanavalin A-like lectin/glucanase (IPR008985)

Growth factor, receptor (IPR009030)

GCC2 and GCC3 (IPR011641)

Laminin G, subdomain 2 (IPR012680)

EGF-like region, conserved site (IPR013032)

EGF calcium-binding (IPR013091)

Concanavalin A-like lectin/glucanase, subgroup (IPR013320)

TNFR/CD27/30/40/95 cysteine-rich region (IPR001368)

C-type lectin (IPR001304)

Complement control module (IPR016060)

C-type lectin-like (IPR016186)

C-type lectin fold (IPR016187)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

RH55424

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

6738

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0031879

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0031879

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0031879

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of SP1070	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of SP1070	Mutagen	Stocks	Known lesion
SP1070^GD139	in vitro construct \| RNAi in vitro construct \| regulatory fusion	3	Yes
SP1070^GD14373	in vitro construct \| RNAi in vitro construct \| regulatory fusion	0	Yes
SP1070^GD2635	in vitro construct \| RNAi in vitro construct \| regulatory fusion	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(2L)Dwee-δ5

Useful duplication

Dp(2;2)C619

Disrupted in

Df(2L)Exel7029

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD139}

P{GD14373}

P{GD2635}

Insertions

Type of insertions

Name

Expression data

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 4 )

Molecular Function

CV term

Evidence

References

Notch binding

inferred from sequence or structural similarity with EMBL:Y14495; protein_id:CAA74835

(FlyBase, 1992-)

calcium ion binding

inferred from electronic annotation with InterPro:IPR001881

(FlyBase Curators et al., 2004-)

receptor activity

inferred from electronic annotation with InterPro:IPR001368

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

cell adhesion

inferred from electronic annotation with InterPro:IPR000421

(FlyBase Curators et al., 2004-)

Cellular Component

CV term

Evidence

References

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

SP1070 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

60132

Drosophila PIMRider - The Drosophila Protein Interaction map

•

521507

Orthologs

Genome-wide drosophilid orthologs

Dana\GF14947

Dere\GG23558

Dgri\GH10705

Dmoj\GI11276

Dper\GL25782

Dpse\GA21569

Dwil\GK24802

Dyak\GE18383

Curated drosophilid orthologs

Dpse\GA21569

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000010271
Anopheles gambiae (Ensembl)
ENSP00000304118
Homo sapiens (Ensembl)
ENSMMUP00000029966
Macaca mulatta (Ensembl)
ENSPTRP00000036296
Pan troglodytes (Ensembl)
WBGene00027821
Caenorhabditis briggsae (Wormbase)
WBGene00018546
Caenorhabditis elegans (Wormbase)
ZDB-GENE-050302-80
Danio rerio (Zfin)
MGI:1928849
Mus musculus (Informatics)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

v1047

v1050

v1051

Genomic Clones ( 2 )

cDNA Clones ( 19 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

LP19335

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0031879

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0031879

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for identity of: SP1070 CG9138 Source for identity of SP1070 CG9138 was sequence comparison (date:000414).

(FlyBase, 1996-)

Source for database merge of

Additional comments

Comments About Role

Comments About Molecular Function

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_135247

RefSeq (Proteins)

•

NP_609091

DDBJ / EMBL / GenBank

•

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Aspartic acid and asparagine hydroxylation site (IPR000152)

Coagulation factor 5/8 type, C-terminal (IPR000421)

Sushi/SCR/CCP (IPR000436)

EGF-like, type 3 (IPR000742)

CUB (IPR000859)

EGF-like, type 2 (IPR001438)

EGF-like calcium-binding (IPR001881)

Low density lipoprotein-receptor, class A, cysteine-rich (IPR002172)

Hyalin (IPR003410)

EGF-like (IPR006209)

EGF (IPR006210)

Galactose-binding like (IPR008979)

Concanavalin A-like lectin/glucanase (IPR008985)

Growth factor, receptor (IPR009030)

GCC2 and GCC3 (IPR011641)

Laminin G, subdomain 2 (IPR012680)

EGF-like region, conserved site (IPR013032)

EGF calcium-binding (IPR013091)

Concanavalin A-like lectin/glucanase, subgroup (IPR013320)

TNFR/CD27/30/40/95 cysteine-rich region (IPR001368)

C-type lectin (IPR001304)

Complement control module (IPR016060)

C-type lectin-like (IPR016186)

C-type lectin fold (IPR016187)

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

60132

DEDB - Drosophila exon database: splicing graphs

•

6738

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0031879

Drosophila PIMRider - The Drosophila Protein Interaction map

•

521507

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0031879

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0031879

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0031879

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0031879

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0031879

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000010271
Anopheles gambiae (Ensembl)
ENSP00000304118
Homo sapiens (Ensembl)
ENSMMUP00000029966
Macaca mulatta (Ensembl)
ENSPTRP00000036296
Pan troglodytes (Ensembl)
WBGene00027821
Caenorhabditis briggsae (Wormbase)
WBGene00018546
Caenorhabditis elegans (Wormbase)
ZDB-GENE-050302-80
Danio rerio (Zfin)
MGI:1928849
Mus musculus (Informatics)

PANTHER - Protein classification by function, families, and pathways

•

FBgn0031879

Synonyms & Secondary IDs ( 8 )

Reported As

Symbol Synonym

CG9138

(Joshi et al., 2007, Zhang et al., 2008)

CT26172

(Hynes and Zhao, 2000, Hynes and Zhao, 2000)

E(br)155

(Zhang et al., 2008)

poly-EGF

(Hynes and Zhao, 2000)

SP1070

uif

(Zhang et al., 2008)

Name Synonym

Enhancer of broad 155

(Zhang et al., 2008)

SP1070

uninflatable

(Zhang et al., 2008)

Secondary FlyBase IDs

References ( 14 )

Supplementary material

Dietzl et al., 2007, Nature 448(7150):: Supplementary Table 1. [FBrf0202579]
Hynes and Zhao, 2000, J. Cell Biol. 150(2):: Table S8. Miscellaneous adhesion protein genes. [FBrf0132112]
Hynes and Zhao, 2000, J. Cell Biol. 150(2):: Table S6. EGF repeat and related adhesion proteins. [FBrf0132110]

Personal communication to FlyBase

Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms.: Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]
Ryder, 2004.4.26, Exelixis CG deletion data.: Exelixis CG deletion data. [FBrf0184335]

Abstract

Zhang et al., 2008, A. Dros. Res. Conf. 49: 236B: Cloning and characterization of uninflatable, a gene required for tracheal inflation. [FBrf0203992]
Joshi et al., 2007, Europ. Dros. Res. Conf. 20: A037: A Cytogenetical Analysis of the 27 C4-28B4 Wnt Region of Drosophila melanogaster. [FBrf0199599]

FlyBase analysis

FlyBase, 1996-, FlyBase inference based on genome sequence analysis.: FlyBase inference based on genome sequence analysis. [FBrf0104946]
FlyBase, 1992-, FlyBase curation: FlyBase curation. [FBrf0105495]

DNA/RNA sequence record

Serano, 2000.2.28, GenBank/EMBL/DDBJ: AF239608: Drosophila melanogaster SP1070 mRNA, complete cds. [FBrf0128051]

Computer file

BDGP Project Members, 2000-, Berkeley Drosophila Genome Project.: Berkeley Drosophila Genome Project. [FBrf0125078]

Automatic genome annotation

FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126705]

Curated genome annotation

Hynes, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126673]