Gene Dmel\Drp1

Help

Close All Open All

General Information

Symbol

Dmel\Drp1

Species

D. melanogaster

Name

Dynamin related protein 1

Annotation symbol

CG3210

Feature type

protein_coding_gene

FlyBase ID

FBgn0026479

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

23A1-23A1

Sequence location

2L:2,581,437..2,585,448 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Dynamin related protein 1 is referred to in FlyBase by the symbol Drp1 (CG3210, FBgn0026479). It has the cytological map location 23A1. Its sequence location is 2L:2581437..2585448. Its molecular function is described as: GTP binding; GTPase activity. It is involved in the biological processes: synaptic vesicle budding; intracellular distribution of mitochondria; mitochondrion organization and biogenesis; vesicle organization and biogenesis; cytokinesis; sperm mitochondrion organization and biogenesis. 15 alleles are reported. The phenotypes of these alleles are annotated with: Nebenkern; mitochondrial derivative; ventral furrow. It has one annotated transcript and one annotated polypeptide.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

23A1-23A1

Limits computationally determined from genome sequence between P{PZ}dpp¹⁰⁶³⁸ and P{EP}EP832

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

22F-23A

Determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones.

(Lloyd et al., 2000)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

In direction of increasing cytology: dpp? oaf? Drp1?

(Littleton and Bellen, 1994)

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Drp1 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Drp1-RA

FBtr0077744

NM_134850

2730

735

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Drp1-PA

FBpp0077424

82.6

735

6.98

NP_608694

AAF51235

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Dynamin central region (IPR000375)

Dynamin, GTPase region (IPR001401)

Dynamin GTPase effector (IPR003130)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

DS02782

(van der Bliek, 1999)

FBcl0297452

(Venken et al., 2006)

Does NOT map to

Identified with

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

5577

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI511778

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0026479

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal

lethal (with Drp1²)

Drp1¹

lethal (with Drp1¹)

Drp1²

lethal | recessive

Drp1²

Other Phenotypes

Allele

neurophysiology defective

Phenotype manifest in

Allele

mitochondrion & primary spermatocyte | germ-line clone

Drp1^KG03815

Nebenkern | germ-line clone

Drp1^KG03815

mitochondrial derivative | germ-line clone

Drp1^KG03815

ventral furrow

Drp1^dsRNA.cGa

Classical Alleles ( 12 )

For All Classical Alleles Show

Allele of Drp1	Mutagen	Stocks	Known lesion
Drp1¹		1	Yes
Drp1²		1	Yes
Drp1^D20	ethyl methanesulfonate	1	--
Drp1^H12		0	--
Drp1^H22		0	--
Drp1^H30		0	--
Drp1^H7		0	Yes
Drp1^KG03815	P-element activity	1	--
Drp1^T1	ethyl methanesulfonate	0	--
Drp1^T26	ethyl methanesulfonate	1	--
Drp1^T9	ethyl methanesulfonate	0	--
Drp1^f02471	piggyBac transposase	1	--

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Drp1	Mutagen	Stocks	Known lesion
Drp1^+t9.4	in vitro construct \| genomic fragment	0	Yes
Drp1^GD10456	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
Drp1^dsRNA.cGa	in vitro construct \| RNAi	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(2L)C144

Df(2L)D20

Df(2L)dpp^d14

Not disrupted in

Df(2L)DTD48

(Gelbart, 1998.10.20)

Df(2L)dpp^d19

(Spencer et al., 1982)

Df(2L)dpp^d21

(Spencer et al., 1982)

Disrupted in

Df(2L)C144

(Ly, 2005.3.2, Littleton and Bellen, 1994, McDaniel, 2006.10.11)

Df(2L)D20

(Littleton and Bellen, 1994)

Df(2L)DTD2

(Spencer et al., 1982)

Df(2L)Exel6008

(Ly, 2005.3.2, Ryder, 2004.4.26)

Df(2L)dpp-H59

(Littleton and Bellen, 1994)

Df(2L)dpp^d14

(Ly, 2005.3.2, Bergstrom et al., 1995)

Df(2L)dpp^d19

(Littleton and Bellen, 1994)

In(2LR)dpp^d23LDTD21^R

(Spencer et al., 1982)

Duplicated in

Dp(2;2)dpp^d13

(Spencer et al., 1982)

Not duplicated in

Dp(2;2)DTD48

(Spencer et al., 1982)

Dp(2;2)dpp^d21

(Spencer et al., 1982)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD10456}

characterization construct

P{Drp1^+t9.4}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{WH}Drp1^f02471

P{SUPor-P}Drp1^KG03815

Related Comments

Please look at the allele reports for the complete phenotype data

Drp1 is required for the normal distribution of mitochondria in spermatocytes and the normal unfurling of the mitochondrial derivatives in early spermatids during sperm formation.

(Aldridge et al., 2007)

Mutants show a distinct temperature sensitive paralysis phenotype.

(Rikhy et al., 2004)

Mutants show a defect in the formation and recruitment of the synaptic vesicle reserve pool.

(Ly et al., 2005)

dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells.

(Echard et al., 2004)

Gene Ontology: Function, Process & Cellular Component ( 9 )

Molecular Function

CV term

Evidence

References

GTP binding

inferred from electronic annotation with InterPro:IPR000375, InterPro:IPR001401, InterPro:IPR003130

(FlyBase Curators et al., 2004-)

GTPase activity

inferred from electronic annotation with InterPro:IPR001401, InterPro:IPR003130

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

cytokinesis