Gene Dmel\fh

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General Information

Symbol

Dmel\fh

Species

D. melanogaster

Name

frataxin homolog

Annotation symbol

CG8971

Feature type

protein_coding_gene

FlyBase ID

FBgn0030092

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

8C14-8C14

Sequence location

X:9,042,127..9,043,092 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene frataxin homolog is referred to in FlyBase by the symbol fh (CG8971, FBgn0030092). It has the cytological map location 8C14. Its sequence location is X:9042127..9043092. Its molecular function is unknown. It is involved in the biological process cellular iron ion homeostasis. 3 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

8C14-8C14

Limits computationally determined from genome sequence between P{EP}EP1450&P{EP}EP1356 and P{EP}EP912^EP912

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

8D-8D

(Canizares-Sales, 1999.11.26)

8C-8D

(determined by in situ hybridisation)

(Canizares et al., 2000)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\fh for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

fh-RA

FBtr0071348

NM_078539

897

190

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

fh-PA

FBpp0071283

20.9

190

6.10

NP_511094

AAF46449

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Frataxin, subgroup (IPR001794)

Frataxin-like (IPR002908)

Frataxin (IPR017789)

MitoDrome - A database of annotated Dmel nuclear genes encoding mitochondrial proteins

•

MTDROME08971

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9W385

UniProtKB/TrEMBL

Maps to

125A12

(Canizares et al., 2000)

Does NOT map to

Identified with

AT09528

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0030092

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0030092

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0030092

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | larval stage | partially, with Scer\GAL4^da.G32

fh^{dsRNA.IR.Scer\UAS}

Other Phenotypes

Allele

short lived | adult stage, with Scer\GAL4^da.G32

fh^{dsRNA.IR.Scer\UAS}

short lived | adult stage, with Scer\GAL4^bbg-C96

fh^{dsRNA.IR.Scer\UAS}

chemical sensitive, with Scer\GAL4^da.G32

fh^{dsRNA.IR.Scer\UAS}

developmental rate defective | larval stage | partially, with Scer\GAL4^da.G32

fh^{dsRNA.IR.Scer\UAS}

Phenotype manifest in

Allele

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of fh	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of fh	Mutagen	Stocks	Known lesion
fh^GD5015	in vitro construct \| RNAi in vitro construct \| regulatory fusion	0	Yes
fh^Scer\UAS.cAa	in vitro construct \| regulatory fusion	1	Yes
fh^{dsRNA.IR.Scer\UAS}	in vitro construct \| regulatory fusion in vitro construct \| RNAi	2	Yes

Aneuploid Aberrations

Useful deficiency

Df(1)9a4-5

Useful duplication

Dp(1;1)L72.2

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD5015}

P{UAS-fh.A}

P{UAS-fh.IR}

Insertions

Type of insertions

Name

Expression data

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 2 )

Molecular Function

CV term

Evidence

References

Biological Process

CV term

Evidence

References

cellular iron ion homeostasis

inferred from sequence or structural similarity with EMBL:U95736

(FlyBase, 1992-)

non-traceable author statement

(Swiss-Prot Project Members, 2000.10.1)

inferred from sequence or structural similarity with UniProtKB:Q16595

(Sardiello et al., 2003.6.11)

Cellular Component

CV term

Evidence

References

mitochondrion

inferred from sequence or structural similarity with EMBL:U95736

(FlyBase, 1992-)

non-traceable author statement

(Swiss-Prot Project Members, 2000.10.1)

inferred from sequence or structural similarity with UniProtKB:Q16595

(Sardiello et al., 2003.6.11)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

fh allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

58300

Orthologs

Genome-wide drosophilid orthologs

Dana\GF19130

Dere\GG19001

Dgri\GH12457

Dmoj\GI15054

Dper\GL26832

Dpse\GA21445

Dsec\GM13678

Dsim\GD16083

Dvir\GJ14708

Dwil\GK10087

Dyak\fh

Curated drosophilid orthologs

Dpse\GA21445

Dsub\fh

Dyak\fh

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000021388
Anopheles gambiae (Ensembl)
ENSBTAP00000001725
Bos taurus (Ensembl)
ENSCAFP00000002795
Canis familiaris (Ensembl)
ENSGALP00000024330
Gallus gallus (Ensembl)
ENSP00000297735
Homo sapiens (Ensembl)
ENSMMUP00000000477
Macaca mulatta (Ensembl)
ENSMODP00000003591
Monodelphis domestica (Ensembl)
ENSPTRP00000035899
Pan troglodytes (Ensembl)
ENSRNOP00000020412
Rattus norvegicus (Ensembl)
ENSXETP00000001259
Xenopus tropicalis (Ensembl)
Q5ZAV6
Oryza sativa (Gramene)
AT4G03240.1
Arabidopsis thaliana (Arabidopsis)
WBGene00025574
Caenorhabditis briggsae (Wormbase)
WBGene00001486
Caenorhabditis elegans (Wormbase)
DDB0191848
Dictyostelium discoideum (Dictybase)
MGI:1096879
Mus musculus (Informatics)
YDL120W
Saccharomyces cerevisiae (Yeastgenome)
SPCC1183.03c
Schizosaccharomyces pombe (Sanger)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

24619

24620

24622

Genomic Clones ( 2 )

cDNA Clones ( 1 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

AT09528

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0030092

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0030092

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for identity of: fh CG8971

(Canizares et al., 2000)

Source for database merge of

Source for merge of: fh BcDNA:AT09528 Source for identity of: fh CG8971 Source for merge of fh BcDNA:AT09528 was shared cDNA (AT09528) (date:030530). Source for identity of fh CG8971 was sequence comparison (date:001203).

(FlyBase, 1996-)

Additional comments

Comments About Role

Comments About Molecular Function

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_078539

RefSeq (Proteins)

•

NP_511094

DDBJ / EMBL / GenBank

•

UniProtKB/Swiss-Prot

•

Q9W385

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Frataxin, subgroup (IPR001794)

Frataxin-like (IPR002908)

Frataxin (IPR017789)

MitoDrome - A database of annotated Dmel nuclear genes encoding mitochondrial proteins

•

MTDROME08971

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

58300

DEDB - Drosophila exon database: splicing graphs

•

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0030092

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0030092

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0030092

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0030092

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0030092

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0030092

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000021388
Anopheles gambiae (Ensembl)
ENSBTAP00000001725
Bos taurus (Ensembl)
ENSCAFP00000002795
Canis familiaris (Ensembl)
ENSGALP00000024330
Gallus gallus (Ensembl)
ENSP00000297735
Homo sapiens (Ensembl)
ENSMMUP00000000477
Macaca mulatta (Ensembl)
ENSMODP00000003591
Monodelphis domestica (Ensembl)
ENSPTRP00000035899
Pan troglodytes (Ensembl)
ENSRNOP00000020412
Rattus norvegicus (Ensembl)
ENSXETP00000001259
Xenopus tropicalis (Ensembl)
Q5ZAV6
Oryza sativa (Gramene)
AT4G03240.1
Arabidopsis thaliana (Arabidopsis)
WBGene00025574
Caenorhabditis briggsae (Wormbase)
WBGene00001486
Caenorhabditis elegans (Wormbase)
DDB0191848
Dictyostelium discoideum (Dictybase)
MGI:1096879
Mus musculus (Informatics)
YDL120W
Saccharomyces cerevisiae (Yeastgenome)
SPCC1183.03c
Schizosaccharomyces pombe (Sanger)

Synonyms & Secondary IDs ( 10 )

Reported As

Symbol Synonym

BcDNA:AT09528

CG8971

(Canizares et al., 2000, )

DFH

(Canizares-Sales, 1997.11.3)

dfh

(Canizares et al., 2000, Anderson et al., 2005)

X25

(Canizares et al., 2000)

Name Synonym

Frataxin

(Navarro et al., 2007)

frataxin

(Anderson et al., 2005)

frataxin homolog

frataxin-like

(Canizares-Sales, 1999.11.26)

Secondary FlyBase IDs

FBgn0062513

References ( 14 )

Research paper

Anderson et al., 2005, Hum. Molec. Genet. 14(22): 3397--3405: RNAi-mediated suppression of the mitochondrial iron chaperone, frataxin, in Drosophila. [FBrf0190823]
Glasscock et al., 2005, Genetics 170(4): 1677--1689: The mei-P26 Gene Encodes a RING Finger B-box Coiled-Coil-NHL Protein That Regulates Seizure Susceptibility in Drosophilia. [FBrf0187640]
Canizares et al., 2000, Gene 256(1-2): 35--42: dfh is a Drosophila homolog of the Friedreich's ataxia disease gene. [FBrf0131265]

Supplementary material

Dietzl et al., 2007, Nature 448(7150):: Supplementary Table 1. [FBrf0202579]
Driscoll and Gerstbrein, 2003, Nature Rev. Genet. 4(3):: Dying for a cause: invertebrate genetics takes on human neurodegeneration. [FBrf0159901]

Personal communication to FlyBase

Sardiello et al., 2003.6.11, MitoDrome: A database of Drosophila melanogaster nuclear genes encoding proteins targeted to the mitochondrion.: MitoDrome: A database of Drosophila melanogaster nuclear genes encoding proteins targeted to the mitochondrion. [FBrf0159903]

Abstract

Navarro et al., 2007, Europ. Dros. Res. Conf. 20: E027: NEUROPATHOLOGY OF DROSOPHILA FRATAXIN GAIN AND LOSS OF FUNCTION. [FBrf0199782]

FlyBase analysis

FlyBase, 1996-, FlyBase inference based on genome sequence analysis.: FlyBase inference based on genome sequence analysis. [FBrf0104946]
FlyBase, 1992-, FlyBase curation: FlyBase curation. [FBrf0105495]

DNA/RNA sequence record

Canizares-Sales, 1999.11.26, GenBank/EMBL/DDBJ: AF208491: Drosophila melanogaster frataxin-like protein (fh) mRNA, partial cds. [FBrf0131492]
Canizares-Sales, 1997.11.3, GenBank/EMBL/DDBJ: AJ002208: Drosophila melanogaster mRNA for DFH gene. [FBrf0132641]

Protein sequence record

Swiss-Prot Project Members, 2000.10.1, UniProtKB: Q9W385: FRATAXIN HOMOLOG PRECURSOR. [FBrf0131799]

Computer file

BDGP Project Members, 2000-, Berkeley Drosophila Genome Project.: Berkeley Drosophila Genome Project. [FBrf0125078]

Automatic genome annotation

FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126705]