A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Gene Dmel\Uba2

General Information
SymbolDmel\Uba2SpeciesD. melanogaster
NameSmt3 activating enzyme 2Annotation symbolCG7528
Feature typeprotein_coding_geneFlyBase IDFBgn0029113
Created / Updated2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)3LRecombination map
Cytogenetic map66B7-66B7Sequence location3L:8,118,423..8,120,919 [-]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene Smt3 activating enzyme 2 is referred to in FlyBase by the symbol Uba2 (CG7528, FBgn0029113). It has the cytological map location 66B7. Its sequence location is 3L:8118423..8120919. Its molecular function is described as: ubiquitin activating enzyme activity; small protein activating enzyme activity; SUMO activating enzyme activity; protein binding. It is involved in the biological processes: ubiquitin cycle; activation of NF-kappaB transcription factor; protein sumoylation; SMT3-dependent protein catabolic process. 4 alleles are reported. The phenotypes of these alleles are annotated with: eye; ommatidium; pigment cell. It has one annotated transcript and one annotated polypeptide.
hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
66B7-66B7  
Limits computationally determined from genome sequence between P{PZ}l(3)0721708223&P{EP}Rac2EP3118 and P{lacW}Nmtj1C7  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
66B6-66B10
Mapped using a high-density filter of P1 clones.  
(Shih et al., 2002)
65B5-65C3
(determined by in situ hybridisation)  
(Long and Griffith, 2000)
66B6-66B11
 
(Shih, 1999.10.8)
66B5-66B11
Cytological location based on P1 blot screening and PCR checking.  
(Shih, 1998.3.17)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
Gene Order (overall orientation not stated)
References
hide Gene Model & Products
Please see the GBrowse view of Dmel\Uba2 for information on other features
detailed view FBtr0076686 FBtr0076687 FBtr0076736 FBtr0076735 FBtr0076734 FBtr0076688 FBpp0076409 FBpp0076410 FBpp0076459 FBpp0076458 FBpp0076457 FBpp0076411 FBti0039534 FBti0033942 FBti0059604 FBti0033602 FBti0059597 FBti0059596 FBti0059598
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
Uba2-RA
FBtr0076734
 NM_080017
  2334
  700
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
Uba2-PA  
FBpp0076457  
77.6  
700  
4.63  
  NP_524756
  AAF50484
Additional Polypeptide Data & Comments
Reported size (kD)
700 (aa)
(Bhaskar et al., 2000)
Comments
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
•
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
Ubiquitin-activating enzyme, E1 (IPR000011)
Ubiquitin-activating enzyme repeat (IPR000127)
UBA/THIF-type NAD/FAD binding fold (IPR000594)
Molybdenum cofactor biosynthesis (IPR009036)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AA201643
 
AA201817
 
AA246406
 
AA263257
 
AA264231
 
AA390338
 
AA390912
 
AA392484
 
AA438602
 
AA439635
 
AA440034
 
AA440929
 
AA540876
 
AA540947
 
AA695661
 
AA735923
 
AA816313
 
AA817331
 
AA941243
 
AA949728
 
AA952079
 
AA952257
 
AA978851
 
AA979053
 
AE003556
AAF50484
 
AF193553
AAF25197
 
AF218864
AAF31704
 
AI455283
 
AI455973
 
AI512119
 
AI512338
 
AI514846
 
AI518245
 
AI519264
 
AI519370
 
AI519402
 
AI531319
 
AI531336
 
AI531785
 
AI532940
 
AI542368
 
AI543040
 
AI543494
 
AI544338
 
AY113361
AAM29366
 
BF495892
 
BG636676
 
BI162944
 
BI166266
 
BI172832
 
BI216136
 
BI227665
 
BI241877
 
BI242787
 
BI352718
 
BI353928
 
BI355205
 
BI355216
 
BI356941
 
BI363226
 
BI368152
 
BI369801
 
BI370784
 
BI371621
 
BI373518
 
BI481562
 
BI482667
 
BI579437
 
BI633818
 
BI637529
 
BI639200
 
BI639896
 
BI640909
 
BP550511
 
BP550512
 
BP550920
 
BP550921
 
BP551526
 
BP551527
 
CK657788
 
CO271975
 
CO276817
 
CO279378
 
CO284980
 
CO311750
 
CO315731
 
CO326131
 
CO329465
 
EC060161
 
EC066415
 
EC076272
 
EC090127
 
EC209067
 
EC211495
 
EC225037
 
EC227119
 
EC227807
 
EC232427
 
EC251074
 
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
    Maps to
    Does NOT map to
    Identified with
    (Long and Griffith, 2000)
    (Shih et al., 2002)
    (BDGP Project Members, 2000-, Bhaskar et al., 2000)
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    hide External Data
    Linkouts
    DEDB - Drosophila exon database: splicing graphs
    •
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
       adult stage
       germarium
      (Shih et al., 2002)
       embryonic stage | stage 6-15
       
      (Shih et al., 2002)
       embryonic stage | stage 6-15
       
      (Shih et al., 2002)
       embryonic stage | embryonic cycle 10..14
       
      (Shih et al., 2002)
       embryonic stage | embryonic cycle 10..14
       
      (Shih et al., 2002)
       adult stage
       oocyte
      (Shih et al., 2002)
      Marker for
        Subcellular Localization
        CV Term
        hide Microarray Data
        Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)
        Untitled Document detailed view FBtr0076686 FBtr0076687 FBtr0076736 FBtr0076735 FBtr0076734 FBtr0076688
        AA978851
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Other Phenotypes
        Allele
        visible, with Scer\GAL4GMR.PF
        Phenotype manifest in
        Allele
        eye, with Scer\GAL4GMR.PF
        ommatidium, with Scer\GAL4GMR.PF
        pigment cell, with Scer\GAL4GMR.PF
        hide Classical Alleles ( 0 )
        For All Classical Alleles Show

        Allele of Uba2ClassMutagenStocksKnown lesion
        hide Alleles Carried on Transgenic Constructs ( 4 )
        For All Alleles Carried on Transgenic Constructs Show

        Allele of Uba2ClassMutagenStocksKnown lesion
        Uba2Act5C.T:Ivir\HA10 Yes
        Uba2C175S.Scer\UAS0 Yes
        Uba2GD122650 Yes
        Uba2Scer\UAS.cLa0 Yes
        hide Aneuploid Aberrations
        Useful deficiency
         
        Useful duplication
         
        Disrupted in
        (Ryder, 2004.4.26)
        hide Transgenic Constructs & Insertions
        Transgenic Constructs
        Type of construct
        Name
        Expression data
        UAS construct
        P{GD12265}
        NA
        P{UAS-Uba2.C175S}
        NA
        P{UAS-Uba2.L}
        NA
        Insertions
        Type of insertions
        Name
        Expression data
        hide Related Comments
        Please look at the allele reports for the complete phenotype data
        hide Gene Ontology: Function, Process & Cellular Component ( 11 )
        hide Molecular Function
        CV term
        Evidence
        References
        SUMO activating enzyme activity
        inferred from sequence or structural similarity
        (Bhaskar et al., 2000)
        non-traceable author statement
        (Bhaskar, 1999.12.27)
        inferred from sequence or structural similarity with EMBL:AF090384; protein_id:AAD12784
        (FlyBase, 1992-)
        protein binding
        inferred from physical interaction with FLYBASE:Aos1; FB:FBgn0029512 AND inferred from physical interaction with FLYBASE:lwr; FB:FBgn0010602
        (Shih et al., 2002)
        small protein activating enzyme activity
        non-traceable author statement
        (Shih, 1999.10.8)
        inferred from physical interaction with FLYBASE:lwr; FB:FBgn0010602 AND inferred from physical interaction with FLYBASE:smt3; FB:FBgn0026170 AND inferred from sequence or structural similarity
        (Shih et al., 2000)
        inferred from sequence or structural similarity
        (FlyBase, 1992-)
        ubiquitin activating enzyme activity
        non-traceable author statement
        (Shih et al., 1998)
        hide Biological Process
        CV term
        Evidence
        References
        SMT3-dependent protein catabolic process
        non-traceable author statement
        (Bhaskar, 1999.12.27)
        positive regulation of NF-kappaB transcription factor activity
        inferred from direct assay
        (Bhaskar et al., 2000)
        protein sumoylation
        inferred from sequence or structural similarity
        (Bhaskar et al., 2000)
        ubiquitin cycle
        non-traceable author statement
        (Shih et al., 1998)
        inferred from electronic annotation with InterPro:IPR000011, InterPro:IPR000127
        (FlyBase Curators et al., 2004-)
        hide Cellular Component
        CV term
        Evidence
        References
        SUMO activating enzyme complex
        inferred from sequence or structural similarity
        (Bhaskar et al., 2000)
        cytoplasm
        inferred from direct assay
        (Shih et al., 2002)
        nucleus
        inferred from direct assay
        (Shih et al., 2002)
        inferred from direct assay
        (Smith et al., 2004)
        hide Sequence Ontology: Class of Gene
        protein_coding_gene
         
        nuclear_gene
         
        hide Interactions & Pathways
        hide Summary of Genetic Interactions
        Interacts with
        Please look at the allele data for full details of the genetic interactions
        Uba2 allele
        Gene
        References
        hide External Data
        Linkouts
        BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
        •
        Drosophila PIMRider - The Drosophila Protein Interaction map
        •
        hide Orthologs
        Genome-wide drosophilid orthologs
        Curated drosophilid orthologs
         
        Linkouts
        InParanoid orthologs - Eukaryotic orthologs
        •
        hide Functional Complementation between Species
        hide Inter-Species Misexpression Data
        Produces phenotype in
        Produces NO phenotype in
        hide Stocks & Reagents
        hide Stocks Listed in FlyBase ( 0 )
        hide Genomic Clones ( 2 )
        hide cDNA Clones ( 77 )
        Please Note
        This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
        cDNA Clones, Fully Sequenced
        BDGP DGC clones
        Other clones
        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones