Gene Dmel\hbs

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General Information

Symbol

Dmel\hbs

Species

D. melanogaster

Name

hibris

Annotation symbol

CG7449

Feature type

protein_coding_gene

FlyBase ID

FBgn0029082

Created / Updated

2004-08-09/2004-08-09

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

51D8-51D8

Sequence location

2R:10,898,903..10,928,901 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene hibris is referred to in FlyBase by the symbol hbs (CG7449, FBgn0029082). It has the cytological map location 51D8. Its sequence location is 2R:10898903..10928901. Its molecular function is unknown. It is involved in the biological processes: regulation of striated muscle development; myoblast fusion. 12 alleles are reported. The phenotypes of these alleles are annotated with 12 unique terms, many of which group under: organ system; primordium; peripheral nervous system; nervous system; adult; myoblast; germ layer; portion of tissue; adult specialised Malpighian tubule cell; midgut chamber; adult segment. It has 2 annotated transcripts and 2 annotated polypeptides.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

51D8-51D8

Limits computationally determined from genome sequence between P{lacW}Rpn6^k00103 and P{PZ}chn⁰²⁰⁶⁴

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

51E1-51E2

(Artero, 1999.12.1)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Overall orientation not stated: aPKC? P{EP}EP2590? P{hbs-lacZ.4}36.1? hbs+ chn?

(Artero et al., 2001)

Gene Model & Products

Please see the GBrowse view of Dmel\hbs for information on other features

Comments on Gene Model

Transposon inserted in intron

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

hbs-RA

FBtr0087413

NM_079963

4717

1228

hbs-RB

FBtr0087414

NM_176175

4738

1235

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

hbs-PA

FBpp0086544

133.6

1228

7.00

NP_524702

AAF58172

hbs-PB

FBpp0086545

134.4

1235

7.00

NP_788355

AAO41390

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

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FBgn0029082

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Immunoglobulin/major histocompatibility complex, conserved site (IPR003006)

Immunoglobulin subtype 2 (IPR003598)

Immunoglobulin subtype (IPR003599)

Fibronectin, type III (IPR003961)

Immunoglobulin-like (IPR007110)

Immunoglobulin (IPR013151)

CD80-like, immunoglobulin C2-set (IPR013162)

Fibronectin, type III-like fold (IPR008957)

Immunoglobulin V-set (IPR013106)

Immunoglobulin-like fold (IPR013783)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

RE70806

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

point mutation

hbs[459]
2R:10,925,673..10,925,673

evidence=experimental
pr_change=K553|hbs-PB,K553|hbs-PA
reported_na_change=A?T
reported_pr_change=K554@
na_change=A10925673T

(Artero et al., 2001)

point mutation

hbs[361]
2R:10,926,645..10,926,645

evidence=experimental
pr_change=L827R|hbs-PB,L827R|hbs-PA
na_change=T10926645G

(Denholm et al., 2003)

point mutation

hbs[2593]
2R:10,926,658..10,926,658

comment=T to A transversion in the splice acceptor results in a retained intron and early translation termination.
evidence=experimental
reported_na_change=T?A
na_change=T10926658A

(Artero et al., 2001)

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

12448

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

embryonic stage | stage 15

embryonic Malpighian tubule | restricted

(Denholm et al., 2003)

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

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FBgn0029082

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

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FBgn0029082

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

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FBgn0029082

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

hbs^c06523

Other Phenotypes

Allele

visible, with Scer\GAL4^sca-537.4

hbs^Scer\UAS.cDa

visible, with Scer\GAL4^da.G32

hbs^Scer\UAS.cDa

visible, with Scer\GAL4^pnr-MD237

hbs^Scer\UAS.cDa

visible, with Scer\GAL4^bi-md653

hbs^Scer\UAS.cDa

visible, with Scer\GAL4^GMR.PF

hbs^Scer\UAS.cDa

Sterility

Allele

fertile

hbs^c06523

Phenotype manifest in

Allele

visceral mesoderm, with Scer\GAL4^Mef2.PR, Scer\GAL4^twi.PB

somatic mesoderm, with Scer\GAL4^Mef2.PR, Scer\GAL4^twi.PB

myoblast, with Scer\GAL4^Mef2.PR, Scer\GAL4^twi.PB

embryonic muscle system, with Scer\GAL4^Mef2.PR, Scer\GAL4^twi.PB

embryonic muscle system (with hbs⁴⁵⁹)

hbs²⁵⁹³

embryonic myoblast (with hbs⁴⁵⁹)

hbs²⁵⁹³

midgut chamber 1 (with hbs⁴⁵⁹)

hbs²⁵⁹³

visceral muscle primordium (with hbs⁴⁵⁹)

hbs²⁵⁹³

embryonic muscle system (with Df(2R)X28)

embryonic myoblast (with Df(2R)X28)

midgut chamber 1 (with Df(2R)X28)

visceral muscle primordium (with Df(2R)X28)

adult Malpighian tubule Type II cell

hbs³⁶¹

embryonic muscle system (with hbs²⁵⁹³)

hbs⁴⁵⁹

embryonic myoblast (with hbs²⁵⁹³)

hbs⁴⁵⁹

midgut chamber 1 (with hbs²⁵⁹³)

hbs⁴⁵⁹

visceral muscle primordium (with hbs²⁵⁹³)

hbs⁴⁵⁹

myoblast, with Scer\GAL4^twi.PG

hbs^Scer\UAS.cDa

myoblast, with Scer\GAL4^da.G32

hbs^Scer\UAS.cDa

embryonic muscle system, with Scer\GAL4^da.G32

hbs^Scer\UAS.cDa

wing margin | distal, with Scer\GAL4^da.G32

hbs^Scer\UAS.cDa

microchaeta & scutum, with Scer\GAL4^da.G32

hbs^Scer\UAS.cDa

eye, with Scer\GAL4^da.G32

hbs^Scer\UAS.cDa

ommatidium, with Scer\GAL4^da.G32

hbs^Scer\UAS.cDa

interommatidial bristle, with Scer\GAL4^da.G32

hbs^Scer\UAS.cDa

embryonic muscle system, with Scer\GAL4^en-e16E

hbs^Scer\UAS.cDa

myoblast, with Scer\GAL4^en-e16E

hbs^Scer\UAS.cDa

embryonic muscle system, with Scer\GAL4^sca-537.4

hbs^Scer\UAS.cDa

myoblast, with Scer\GAL4^sca-537.4

hbs^Scer\UAS.cDa

microchaeta & scutum, with Scer\GAL4^sca-537.4

hbs^Scer\UAS.cDa

eye, with Scer\GAL4^sca-537.4

hbs^Scer\UAS.cDa

ommatidium, with Scer\GAL4^sca-537.4

hbs^Scer\UAS.cDa

interommatidial bristle, with Scer\GAL4^sca-537.4

hbs^Scer\UAS.cDa

embryonic muscle system | dorsal, with Scer\GAL4^pnr-MD237

hbs^Scer\UAS.cDa

myoblast, with Scer\GAL4^pnr-MD237

hbs^Scer\UAS.cDa

microchaeta & scutum, with Scer\GAL4^pnr-MD237

hbs^Scer\UAS.cDa

wing margin | distal, with Scer\GAL4^bi-md653

hbs^Scer\UAS.cDa

eye, with Scer\GAL4^GMR.PF

hbs^Scer\UAS.cDa

ommatidium, with Scer\GAL4^GMR.PF

hbs^Scer\UAS.cDa

interommatidial bristle, with Scer\GAL4^GMR.PF

hbs^Scer\UAS.cDa

Classical Alleles ( 4 )

For All Classical Alleles Show

Allele of hbs	Mutagen	Stocks	Known lesion
hbs²⁵⁹³	ethyl methanesulfonate	0	--
hbs³⁶¹	ethyl methanesulfonate	0	--
hbs⁴⁵⁹	ethyl methanesulfonate	0	--
hbs^c06523	piggyBac transposase	1	--

Alleles Carried on Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Allele of hbs	Mutagen	Stocks	Known lesion
hbs^GD14406	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
hbs^GD2566	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
hbs^GD70	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
hbs^MtnA.PD	in vitro construct \| regulatory fusion	0	Yes
hbs^Scer\UAS.cAa	in vitro construct \| regulatory fusion	0	Yes
hbs^Scer\UAS.cDa	in vitro construct \| regulatory fusion	0	Yes
hbs^{ΔECD.Scer\UAS}	in vitro construct \| deletion	0	Yes
hbs^{ΔICD.Scer\UAS}	in vitro construct \| deletion	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(2R)XTED1

Useful duplication

Dp(2;2)SMG45

Disrupted in

Df(2R)EP2590-Δ1

(Dworak et al., 2001)

Df(2R)EP2590-Δ14

(Dworak et al., 2001, Denholm et al., 2003)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

reporter construct

P{hbs-lacZ.4}

Yes

UAS construct

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{PB}hbs^c06523

P{hbs-lacZ.4}36.1

Related Comments

Please look at the allele reports for the complete phenotype data

Mutants exhibit a phenotype in which the A/P cone cell contact is improperly maintained through the end of pattern formation.

(Grillo-Hill and Wolff, 2003)

Embryos mutant for hbs or overexpressing hbs show a partial myoblast fusion block.

(Artero and Baylies, 2001.5.25)

Gene Ontology: Function, Process & Cellular Component ( 3 )

Molecular Function

CV term

Evidence

References

Biological Process

CV term

Evidence

References

myoblast fusion

traceable author statement

(Dworak and Sink, 2002)

regulation of striated muscle development

inferred from genetic interaction with FLYBASE:sns; FB:FBgn0024189 AND inferred from mutant phenotype

(Artero et al., 2001)

inferred from mutant phenotype

(Dworak et al., 2001)

Cellular Component

CV term

Evidence

References

plasma membrane

inferred from sequence or structural similarity with NCBI_gi:4758822

(FlyBase, 1992-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

hbs allele

Gene

References

hbs²⁵⁹³

sns

(Artero et al., 2001)

hbs⁴⁵⁹

sns

(Artero et al., 2001)

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

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68850

Orthologs

Genome-wide drosophilid orthologs

Dana\GF12310

Dere\GG20496

Dgri\GH21109

Dmoj\GI18711

Dper\GL17448

Dpse\GA20361

Dsec\GM21588

Dsim\GD11096

Dvir\GJ21730

Dwil\GK19700

Dyak\GE13629

Curated drosophilid orthologs

Dpse\GA20361

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

WBGene00029565
Caenorhabditis briggsae (Wormbase)
WBGene00007750
Caenorhabditis elegans (Wormbase)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 5 )

VDRC

v40896

w¹¹¹⁸ P{GD2566}v40896

v40898

w¹¹¹⁸; P{GD2566}v40898

v9471

w¹¹¹⁸; P{GD70}v9471/CyO

v27065

w¹¹¹⁸; P{GD14406}v27065/TM3

Bloomington

17767

w¹¹¹⁸; PBac{PB}hbs^c06523

Genomic Clones ( 2 )

cDNA Clones ( 13 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

RE70806

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

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FBgn0029082

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

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FBgn0029082

Antibody Information

Other Information

Discoverer

Etymology

Etymology: The free myoblasts of the hbs mutant phenotype represent rebelliousness, insolence, and transgression of rules (in this case in the mesodermal "society"), the three qualities that the minor Greek Goddess "hibris" represents.

(Artero and Baylies, 2001.5.25)

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: hbs CG7449

(Artero, 2000.3.29)

Source for merge of: hbs icon

(Grillo-Hill, 2005.3.28)

Additional comments

Comments About Role

hbs appears to function as a dose dependant regulator of myoblast fusion.

(Artero et al., 2001)

hbs appears to be a component of the molecular machinery that facilitates both myoblast and myotube guidance. hbs may also be involved in cell-cell contact events in the eye, bristle sensory oprgan and wing development.

(Dworak et al., 2001)

Comments About Molecular Function

ChEST reveals this is a target of Mef2.

(Junion et al., 2005)

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

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DDBJ / EMBL / GenBank