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Gene Dmel\onecut

General Information
Symbol	Dmel\onecut	Species	D. melanogaster
Name	onecut	Annotation symbol	CG1922
Feature type	protein_coding_gene	FlyBase ID	FBgn0028996
Created / Updated	2003-12-02/2003-12-02
Genomic Location
Chromosome (arm)	4	Recombination map
Cytogenetic map	102C2-102C2	Sequence location	4:619,180..628,119 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol onecut (CG1922, FBgn0028996). It has the cytological map location 102C2. Its sequence location is 4:619180..628119. Its molecular function is described as: RNA polymerase II transcription factor activity; DNA binding; transcription regulator activity; sequence-specific DNA binding; transcription factor activity. It is involved in the biological processes: transcription; regulation of transcription, DNA-dependent. 8 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.
External Summaries
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 102C2-102C2   Limits computationally determined from genome sequence between P{SUPor-P}AsatorKG05051&P{SUPor-P}KG01424 and PBac{3HPy+}C024
Experimentally Determined Cytological Location
Cytogenetic map Notes References 102C6-102C6   (Nguyen, 1999.7.14)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References Overall orientation not stated: Eph- onecut+ (Nguyen et al., 2000)
Gene Model & Products
Please see the GBrowse view of Dmel\onecut for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) onecut-RA FBtr0089104  NM_080103   6587   1081
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein onecut-PA   FBpp0088173   117.7   1081   5.02     NP_524842   AAF59330
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0028996
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Homeobox (IPR001356) Homeodomain protein CUT (IPR003350) Homeodomain-related (IPR012287) Homeodomain-like (IPR009057)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE003843 AAF59330   AF169227 AAF25796   AI404274   AI517346   AW941087   AY058476 AAL13705   BI239515   BI242906   BT024278   CO176947   CO179033   CO179065   CO179090   CO187130   CO189523   CO191103   CO268454   CO269140   CO269331   CO271428   CO271606   CO275154   CO275158   CO275609   CO277479   CO279254   CO279363   CO289309   CO289555   CO290222   CO292043   CO292412   CO294182   CO297591   CO299103   CO299572   CO305289   CO317846   CO317997   CO319839   CO322051   CO322666   CO324604   CO324887   CO329244   CO330047   CO330274   CO330275   CO331358   CO333927   CO342033   CZ478012   DQ017414 AAY82196   DQ017415 AAY82197   EC057836   EC060085   EC062231   EC063227   EC063562   EC065820   EC069001   EC069829   EC072408   EC075603   EC078564   EC083008   EC086300   EC087314   EC195894   EC196424   EC197067   EC197428   EC199228   EC200556   EC204444   EC204919   EC206628   EC207665   EC208605   EC208836   EC209138   EC209511   EC210139   EC211928   EC212211   EC213829   EC215521   EC215753   EC215861   EC216292   EC216304   EC216786   EC217453   EC219991   EC223423   EC228052   EC230282   EC232249   EC232665   EC232805   EC235015   EC238923   EC239309   EC240543   EC241044   EC243554   EC246976   EC250038   EC250727   EC251062   EC253251   EC254229   EL874493   EL874494   EL874565   EL874566   EL874639
UniProtKB/Swiss-Prot	Q9NJB5
UniProtKB/TrEMBL	Q3YND8 Q95TW5
Maps to
Does NOT map to
Identified with	GH28062 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 13598
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AI517346
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0028996 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0028996 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0028996 FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for onecut Stage(s) 9-10 Stage(s) 11-12 Stage(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable onecute00113 lethal (with onecutx122) onecutx49 lethal (with onecutx49) onecutx122 lethal | recessive onecutx122 onecutx49 Sterility Allele fertile onecute00113 Phenotype manifest in Allele
Classical Alleles ( 5 )
For All Classical Alleles Show
Allele of onecut Class Mutagen Stocks Known lesion onecute00113 piggyBac transposase 2 -- onecutx122 Δ2-3 0 Yes onecutx49 Δ2-3 0 Yes onecutx652 0 Yes onecut[EY30004] P-element activity 1 --
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of onecut Class Mutagen Stocks Known lesion onecutGD4475 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes onecutScer\UAS.cNa in vitro construct | regulatory fusion 0 Yes onecutcopia.LTR in vitro construct | regulatory fusion 0 Yes
Aneuploid Aberrations
Partially disrupted in	Df(4)Ephx652 (Boyle et al., 2006)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4475} NA P{UAS-onecut.N} NA characterization construct P{UAS-en::onecut.N} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{RB}onecute00113 NA P{hsp26-pt-T}P114 NA insertion of mobile activating element P{EPgy2}onecutEY30004 NA
Related Comments
	Please look at the allele reports for the complete phenotype data
Gene Ontology: Function, Process & Cellular Component ( 9 )
Molecular Function
CV term Evidence References
DNA binding non-traceable author statement (Swiss-Prot Project Members, 2000.10.1)
RNA polymerase II transcription factor activity inferred from sequence or structural similarity (Nguyen, 1999.7.14)
sequence-specific DNA binding inferred from electronic annotation with InterPro:IPR001356 (FlyBase Curators et al., 2004-)
transcription factor activity inferred from electronic annotation with InterPro:IPR001356 (FlyBase Curators et al., 2004-)
transcription regulator activity non-traceable author statement (Swiss-Prot Project Members, 2000.10.1)
Biological Process
CV term Evidence References
regulation of transcription, DNA-dependent non-traceable author statement (Swiss-Prot Project Members, 2000.10.1) inferred from electronic annotation with InterPro:IPR001356 (FlyBase Curators et al., 2004-)
transcription inferred from sequence or structural similarity (Nguyen, 1999.7.14)
Cellular Component
CV term Evidence References
nucleoplasm inferred from electronic annotation (Roth and Foulger, 2002.4.24)
nucleus inferred from sequence or structural similarity (Nguyen, 1999.7.14) non-traceable author statement (Swiss-Prot Project Members, 2000.10.1) inferred from electronic annotation with InterPro:IPR001356 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions onecut allele Gene References
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 69903
Orthologs
Genome-wide drosophilid orthologs	Dana\GF20615 Dere\GG16442 Dgri\GH24019 Dmoj\GI14052 Dper\GL18146 Dpse\GA15133 Dsec\GM26776 Dsim\GD24376 Dvir\GJ19930 Dwil\GK13692 Dyak\GE14509
Curated drosophilid orthologs	Dpse\GA15133
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000017372 Anopheles gambiae (Ensembl) ENSBTAP00000010868 Bos taurus (Ensembl) ENSCAFP00000023336 Canis familiaris (Ensembl) NEWSINFRUP00000180287 Takifugu rubripes (Ensembl) ENSGALP00000004818 Gallus gallus (Ensembl) ENSP00000302630 Homo sapiens (Ensembl) ENSMMUP00000001910 Macaca mulatta (Ensembl) ENSPTRP00000012119 Pan troglodytes (Ensembl) ENSRNOP00000010739 Rattus norvegicus (Ensembl) GSTENP00028516001 Tetraodon nigroviridis (Ensembl) ENSXETP00000017817 Xenopus tropicalis (Ensembl) WBGene00028100 Caenorhabditis briggsae (Wormbase) WBGene00015934 Caenorhabditis elegans (Wormbase) MGI:1196423 Mus musculus (Informatics)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 6 )
Harvard	- PBac{RB}onecute00113
Bloomington	9566 w*; P{hsp26-pt-T}P114 22328 y1; P{EPgy2}onecutEY30004 17816 w1118; PBac{RB}onecute00113
VDRC	v10663 w1118 P{GD4475}v10663 v10665 w1118; P{GD4475}v10665
Genomic Clones ( 1 )
	BACR44L03
cDNA Clones ( 50 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH28062 IP14429
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH23921 RE40414 RE36014
Other clones	EK116614 EK269960 EK194339 IP14629 EK294849 EK125235 EK247964 EK206254 EK026519 EK269461 EK073364 EK170137 EK093716 EK281010 EK070734 EK071321 IP14529 EK058237 EK051719 EK058320 EK188614 EK294426 EK175832 EK050738 EK133908 EK058355 EK299358 EK200131 EK292334 EK044448 EK103423 EK199050 IP14729 EK174516 EK091501 EK294848 EK116622 EK104021 EK134149 EK118035