Gene Dmel\Tbp-1

(BDGP Project Members, 2000-)

PANTHER - Protein classification by function, families, and pathways

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FBgn0028684

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

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Peptidase S16, Lon protease (IPR001984)

AAA+ ATPase, core (IPR003593)

AAA ATPase, core (IPR003959)

AAA ATPase, conserved site (IPR003960)

26S proteasome subunit P45 (IPR005937)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

GH12068

GM06024

LD22987

(Hoelzl, 1999.4.23, Holzl et al., 2000)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

DEDB - Drosophila exon database: splicing graphs

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10478

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI134568

External Data & Images

Show all FlyExpress processed images for Tbp-1

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

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FBgn0028684

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

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FBgn0028684

FlyExpress - Embryonic expression images (BDGP data)

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Stage(s) 4-6

Stage(s) 11-12

Stage(s) 13-16

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | recessive

Tbp-1^04210b

Phenotype manifest in

Allele

Classical Alleles ( 3 )

For All Classical Alleles Show

Allele of Tbp-1	Mutagen	Stocks	Known lesion
Tbp-1^04210b	P-element activity	1	--
Tbp-1^DG19608	P-element activity	1	--
Tbp-1^c01875	piggyBac transposase	0	--

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Tbp-1	Class	Mutagen	Stocks	Known lesion
Tbp-1^GD6960		in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
Tbp-1^dsRNA.cWa		in vitro construct \| RNAi	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(3R)mbc-R.1

Useful duplication

Dp(3;3)M95A⁺13

Disrupted in

Df(3R)BSC489

(Christensen et al., 2008.4.15)

Df(3R)BSC490

(Christensen et al., 2008.4.15)

Df(3R)Exel9013

(Ryder, 2004.4.26)

Not disrupted in

Df(3R)c(3)G-2

(BDGP Project Members, 1994-1999)

Df(3R)sbd105

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD6960}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{PB}Tbp-1^c01875

P{PTT-GB}CB02343

P{wHy}Tbp-1^DG19608

insertion of enhancer trap

P{PZ}Tbp-1^04210b

Yes

Related Comments

Please look at the allele reports for the complete phenotype data

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

Gene Ontology: Function, Process & Cellular Component ( 12 )

Molecular Function

CV term

Evidence

References

ATP binding

inferred from electronic annotation with InterPro:IPR003959, InterPro:IPR003960

ATP-dependent peptidase activity

inferred from electronic annotation with InterPro:IPR001984

ATPase activity

inferred from sequence or structural similarity with SGD_LOCUS:RPT5; SGD:S0005643

endopeptidase activity

inferred from sequence or structural similarity

non-traceable author statement

(Fermin, 1999.3.11)

inferred from direct assay

serine-type endopeptidase activity

inferred from electronic annotation with InterPro:IPR001984

protein catabolic process

Biological Process

CV term

Evidence

References

cellular process

inferred from mutant phenotype

(Boutros et al., 2004)

inferred from electronic annotation with InterPro:IPR005937

proteolysis

inferred from direct assay AND inferred from sequence or structural similarity with HGNC_gene:PSMC3; HGNC:9549; OMIM:186852 AND inferred from sequence or structural similarity with SGD_LOCUS:RPT5; SGD:S0005643

Cellular Component

CV term

Evidence

References

cytoplasm

inferred from electronic annotation with InterPro:IPR005937

nucleus

inferred from electronic annotation with InterPro:IPR005937

proteasome regulatory particle

inferred from direct assay AND inferred from sequence or structural similarity with HGNC_gene:PSMC3; HGNC:9549; OMIM:186852

proteasome regulatory particle, base subcomplex

inferred from sequence or structural similarity

non-traceable author statement

(Fermin, 1999.3.11)

non-traceable author statement

(Hoelzl, 1999.4.23)

inferred from sequence or structural similarity with SGD_LOCUS:RPT5; SGD:S0005643

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Tbp-1 allele

Gene

References

External Data

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

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67743

Orthologs

Genome-wide drosophilid orthologs

Dana\GF16259

Dere\GG11226

Dgri\GH23603

Dmoj\GI10364

Dper\GL23259

Dpse\GA10280

Dsec\GM26527

Dsim\GD21035

Dvir\GJ22583

Dwil\GK22416

Dyak\Tbp-1

Curated drosophilid orthologs

Dpse\GA10280

Dyak\Tbp-1

y¹ w^67c23; P{wHy}CG10225^DG19608 P{wHy}Tbp-1^DG19608

InParanoid orthologs - Eukaryotic orthologs

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ENSANGP00000007334
Anopheles gambiae (Ensembl)
ENSBTAP00000028983
Bos taurus (Ensembl)
ENSCAFP00000012802
Canis familiaris (Ensembl)
ENSCINP00000007046
Ciona intestinalis (Ensembl)
NEWSINFRUP00000158943
Takifugu rubripes (Ensembl)
ENSGALP00000013148
Gallus gallus (Ensembl)
ENSP00000298852
Homo sapiens (Ensembl)
ENSMMUP00000020482
Macaca mulatta (Ensembl)
ENSMODP00000024839
Monodelphis domestica (Ensembl)
ENSPTRP00000006195
Pan troglodytes (Ensembl)
ENSRNOP00000015910
Rattus norvegicus (Ensembl)
GSTENP00032803001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000017362
Xenopus tropicalis (Ensembl)
P46465
Oryza sativa (Gramene)
Q8W421
Oryza sativa (Gramene)
AT3G05530.1
Arabidopsis thaliana (Arabidopsis)
WBGene00033745
Caenorhabditis briggsae (Wormbase)
WBGene00004505
Caenorhabditis elegans (Wormbase)
ZDB-CDNA-040625-52
Danio rerio (Zfin)
ZDB-GENE-030131-666
Danio rerio (Zfin)
DDB0232967
Dictyostelium discoideum (Dictybase)
MGI:1098754
Mus musculus (Informatics)
YOR117W
Saccharomyces cerevisiae (Yeastgenome)
SPAC3A11.12c
Schizosaccharomyces pombe (Sanger)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

VDRC

v39666

w¹¹¹⁸; P{GD6960}v39666

Bloomington

21697

11625

ry⁵⁰⁶ P{PZ}04210a P{PZ}Tbp-1^04210b/TM3, ry^RK Sb¹ Ser¹

Genomic Clones ( 2 )

cDNA Clones ( 35 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

GH12068

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo