Gene Dmel\pUf68

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General Information

Symbol

Dmel\pUf68

Species

D. melanogaster

Name

poly U binding factor 68kD

Annotation symbol

CG12085

Feature type

protein_coding_gene

FlyBase ID

FBgn0028577

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

62A2-62A3

Sequence location

3L:1,496,851..1,502,589 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene poly U binding factor 68kD is referred to in FlyBase by the symbol pUf68 (CG12085, FBgn0028577). It has the cytological map location 62A2-62A3. Its sequence location is 3L:1496851..1502589. Its molecular function is described as: poly(U) binding; mRNA binding; nucleotide binding. It is involved in the biological processes: nuclear mRNA splicing, via spliceosome; alternative nuclear mRNA splicing, via spliceosome; cystoblast division; mRNA splice site selection; regulation of alternative nuclear mRNA splicing, via spliceosome; regulation of cell cycle. 11 alleles are reported. The phenotypes of these alleles are annotated with 18 unique terms, many of which group under: organ system; adult segment; peripheral nervous system; adult mesothoracic segment; egg; nervous system; thoracic segment; imaginal precursor; contractile ring; dorsal thoracic disc; karyosome. It has 4 annotated transcripts and 4 annotated polypeptides.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

62A2-62A3

Limits computationally determined from genome sequence between P{EP}rho^EP3704&P{PZ}l(3)06226⁰⁶²²⁶ and P{PZ}dlt⁰⁴²⁷⁶

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\pUf68 for information on other features

Comments on Gene Model

EST data support existence of multiple transcripts due to variable use of mini-exons.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

pUf68-RA

FBtr0072709

NM_080384

2384

637

pUf68-RB

FBtr0072710

NM_167880

2476

545

pUf68-RC

FBtr0072707

NM_167881

2457

545

pUf68-RD

FBtr0072708

NM_167882

2704

545

Additional Transcript Data & Comments

Reported size (kB)

2.5 (unknown)

(Van Buskirk and Schupbach, 2002)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

pUf68-PA

FBpp0072593

67.9

637

5.76

NP_525123

AAF47501

pUf68-PB

FBpp0072594

58.0

545

4.95

NP_728610

AAG22221

pUf68-PC

FBpp0072591

58.0

545

4.95

NP_728611

AAF47502

pUf68-PD

FBpp0072592

58.0

545

4.95

NP_728612

AAG22222

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0028577

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

RNA recognition motif, RNP-1 (IPR000504)

Poly-U binding splicing factor, half-pint (IPR006532)

Nucleotide-binding, alpha-beta plait (IPR012677)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q8T6B9

UniProtKB/TrEMBL

Maps to

DS02734

(Van Buskirk and Schupbach, 2002)

Does NOT map to

Identified with

(BDGP Project Members, 2000-)

LD27486

(Van Buskirk and Schupbach, 2002)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

1937

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

embryonic stage | 0-2

whole organism

(Van Buskirk and Schupbach, 2002)

embryonic stage | 4-6

whole organism

(Van Buskirk and Schupbach, 2002)

adult stage | female

ovary

(Van Buskirk and Schupbach, 2002)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

oogenesis stage

ovary

(Van Buskirk and Schupbach, 2002)

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AA948827

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0028577

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0028577

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0028577

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for pUf68

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for pUf68

Stage(s) 7-8

Stage(s) 11-12

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

pUf68^CA06961

lethal (with Df(3L)Ar14-8)

pUf68⁹

lethal | recessive, with Scer\GAL4[-]:

pUf68^EP3058

lethal | pupal stage (with Df(3L)Ar14-8), with Scer\GAL4[-]:

pUf68^EP3058

lethal | pharate adult stage | recessive, with Scer\GAL4[-]:

pUf68^EP3058

Other Phenotypes

Allele

large body | pupal stage | recessive, with Scer\GAL4[-]:

pUf68^EP3058

large body | pharate adult stage | recessive, with Scer\GAL4[-]:

pUf68^EP3058

cell death increase | larval stage | recessive, with Scer\GAL4[-]:

pUf68^EP3058

mitotic cell cycle defective | recessive

pUf68¹³

mitotic cell cycle defective | embryonic stage | recessive, with Scer\GAL4[-]:

pUf68^EP3058

cytokinesis defective | embryonic stage | recessive, with Scer\GAL4[-]:

pUf68^EP3058

developmental rate defective (with Df(3L)Ar14-8), with Scer\GAL4[-]:

pUf68^EP3058

developmental rate defective | recessive, with Scer\GAL4[-]:

pUf68^EP3058

Sterility

Allele

female sterile (with Df(3L)Ar14-8)

pUf68¹³

female sterile | recessive

pUf68⁹

male sterile (with Df(3L)Ar14-8)

pUf68¹³

male sterile | recessive

pUf68⁹

fertile

pUf68¹³

Phenotype manifest in

Allele

egg chamber (with Df(3L)Ar14-8)

pUf68¹³

nurse cell (with Df(3L)Ar14-8)

pUf68¹³

egg chamber

ring canal

pUf68¹³

nurse cell & nuclear chromosome

ovary | germ-line clone

pUf68³⁸

karyosome

pUf68⁹

dorsal appendage

pUf68⁹

egg

pUf68⁹

melanotic mass (with Df(3L)Ar14-8), with Scer\GAL4[-]:

pUf68^EP3058

leg, with Scer\GAL4[-]:

pUf68^EP3058

wing, with Scer\GAL4[-]:

pUf68^EP3058

sex comb, with Scer\GAL4[-]:

pUf68^EP3058

scutellar bristle, with Scer\GAL4[-]:

pUf68^EP3058

dorsal mesothoracic disc, with Scer\GAL4[-]:

pUf68^EP3058

eye disc, with Scer\GAL4[-]:

pUf68^EP3058

mitosis & nuclear chromosome, with Scer\GAL4[-]:

pUf68^EP3058

contractile ring, with Scer\GAL4[-]:

pUf68^EP3058

eye, with Scer\GAL4^GMR.PF

pUf68^Scer\UAS.cQa

ommatidium, with Scer\GAL4^GMR.PF

pUf68^Scer\UAS.cQa

eye disc & larva, with Scer\GAL4^GMR.PF

pUf68^Scer\UAS.cQa

wing, with Scer\GAL4^en-e16E

pUf68^Scer\UAS.cQa

wing vein, with Scer\GAL4^en-e16E

pUf68^Scer\UAS.cQa

dorsal mesothoracic disc, with Scer\GAL4^en-e16E

pUf68^Scer\UAS.cQa

ovary

pUf68^unspecified

Classical Alleles ( 8 )

For All Classical Alleles Show

Allele of pUf68	Class	Mutagen	Stocks	Known lesion
pUf68¹³	hypomorph	P-element activity	0	Yes
pUf68³⁸			0	--
pUf68⁷			0	--
pUf68⁹	hypomorph	P-element activity	0	Yes
pUf68^CA06961		P-element activity	0	Yes
pUf68^EP3058		P-element activity	2	Yes
pUf68^EY07952		P-element activity	1	--
pUf68^unspecified			0	--

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of pUf68	Mutagen	Stocks	Known lesion
pUf68^GD7475	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
pUf68^Scer\UAS.cQa	in vitro construct \| regulatory fusion	0	Yes
pUf68^{otu.T:Avic\GFP}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(3L)Ar14-8

Disrupted in

Df(3L)Ar14-8

(Van Buskirk and Schupbach, 2002, Quinn et al., 2004)

Df(3L)BSC289

(Christensen et al., 2007.5.8)

Df(3L)Exel6087

(Ryder, 2004.4.26)

Not disrupted in

Df(3L)Aprt-133

(Van Buskirk and Schupbach, 2002)

Df(3L)ru-22

(Van Buskirk and Schupbach, 2002)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD7475}

P{UAS-pUf68.Q}

characterization construct

P{otu-hfp-GFP}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{EP-Δ13}pUf68¹³

P{EP-Δ9}pUf68⁹

P{PTT-GA}pUf68^CA06961

insertion of mobile activating element

P{EPgy2}pUf68^EY07952

P{EP}pUf68^EP3058

Related Comments

Please look at the allele reports for the complete phenotype data

pUf68 mutant larvae are produced at a normal frequency, however they undergo an extended larval stage and are significantly larger than wild type. Imaginal discs from hypomorphic pUf68 mutant larvae have increased S phases. Despite completing metamorphosis, pUf68 pupae die in their pupal case. These pupae are large, with malformed legs and consistent duplication of scutellar bristles.

(Quinn et al., 2002)