Gene Dmel\ics

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General Information

Symbol

Dmel\ics

Species

D. melanogaster

Name

icarus

Annotation symbol

CG9031

Feature type

protein_coding_gene

FlyBase ID

FBgn0028546

Created / Updated

2003-12-01/2007-06-15

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

34C6-34C6

Sequence location

2L:13,639,527..13,641,271 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene icarus is referred to in FlyBase by the symbol ics (CG9031, FBgn0028546). It has the cytological map location 34C6. Its sequence location is 2L:13639527..13641271. Its molecular function is described as: small GTPase regulator activity; protein binding. It is involved in the biological processes: apposition of dorsal and ventral imaginal disc-derived wing surfaces; negative regulation of JNK cascade; negative regulation of JNK activity. 3 alleles are reported. The phenotype of these alleles is annotated with wing blade. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

34C6-34C6

Limits computationally determined from genome sequence between P{PZ}kuz⁰³⁷⁸²&P{lacW}kuz^k14817 and P{lacW}CG7364^k07245

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

In direction of increasing cytology: kuz+ Rsu-1- CG18507+

(Ashburner et al., 1999)

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\ics for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

ics-RA

FBtr0080511

NM_135821

1054

283

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

ics-PA

FBpp0080089

31.9

283

7.71

NP_609665

AAF53320

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0028546

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Leucine-rich repeat (IPR001611)

Leucine-rich repeat, typical subtype (IPR003591)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

FBcl0297539

(Ashburner et al., 1999.10.12)

Does NOT map to

Identified with

(Ashburner et al., 1999.10.12)

LD13077

(Ashburner et al., 1999, Ashburner et al., 1999.10.12)

LD43891

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

7548

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI512154

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0028546

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0028546

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

ics^BG02577

Other Phenotypes

Allele

visible | adult stage | recessive

ics^BG02577

Sterility

Allele

fertile

ics^BG02577

Phenotype manifest in

Allele

wing blade

ics^BG02577

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of ics	Class	Mutagen	Stocks	Known lesion
ics^BG02577	loss of function	P-element activity	1	Yes

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of ics	Class	Mutagen	Stocks	Known lesion
ics^GD14099		in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
ics^{MtnA.T:SV5\V5,T:Zzzz\His6}		in vitro construct \| coding region fusion in vitro construct \| regulatory fusion	0	Yes

Aneuploid Aberrations

Useful deficiency

In(2L)b82a1

Useful duplication

Dp(2;2)GYL

Disrupted in

Df(2L)Exel7058

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD14099}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{GT1}ics^BG02577

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 5 )

Molecular Function

CV term

Evidence

References

protein binding

inferred from electronic annotation with InterPro:IPR001611

(FlyBase Curators et al., 2004-)

small GTPase regulator activity

inferred from sequence or structural similarity

(Ashburner et al., 1999)

Biological Process

CV term

Evidence

References

apposition of dorsal and ventral imaginal disc-derived wing surfaces

inferred from mutant phenotype

(Kadrmas et al., 2004)

negative regulation of JNK activity

inferred from mutant phenotype

(Kadrmas et al., 2004)

negative regulation of JNK cascade

inferred from genetic interaction with FLYBASE:msn; FB:FBgn0010909 inferred from genetic interaction with FLYBASE:bsk; FB:FBgn0000229

(Kadrmas et al., 2004)

Cellular Component

CV term

Evidence

References

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

ics allele

Gene

References

unspecified

msn

(Kadrmas et al., 2004)

bsk

(Kadrmas et al., 2004)

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

60808

Orthologs

Genome-wide drosophilid orthologs

Dana\GF21702

Dere\GG10189

Dgri\GH13878

Dmoj\GI10104

Dper\GL25689

Dpse\GA25391

Dsec\GM25411

Dsim\GD22052

Dvir\GJ18367

Dwil\GK24552

Dyak\GE11514

Curated drosophilid orthologs

Dpse\GA21491

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000012358
Anopheles gambiae (Ensembl)
ENSBTAP00000027620
Bos taurus (Ensembl)
ENSCAFP00000006853
Canis familiaris (Ensembl)
ENSCINP00000016323
Ciona intestinalis (Ensembl)
NEWSINFRUP00000136160
Takifugu rubripes (Ensembl)
ENSGALP00000014173
Gallus gallus (Ensembl)
ENSP00000343559
Homo sapiens (Ensembl)
ENSMMUP00000021140
Macaca mulatta (Ensembl)
ENSMODP00000012449
Monodelphis domestica (Ensembl)
ENSPTRP00000003935
Pan troglodytes (Ensembl)
ENSRNOP00000024086
Rattus norvegicus (Ensembl)
GSTENP00009444001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000016230
Xenopus tropicalis (Ensembl)
WBGene00031312
Caenorhabditis briggsae (Wormbase)
WBGene00007924
Caenorhabditis elegans (Wormbase)
DDB0183955
Dictyostelium discoideum (Dictybase)
MGI:103040
Mus musculus (Informatics)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

VDRC

v42188

w¹¹¹⁸; P{GD14099}v42188

v42189

w¹¹¹⁸; P{GD14099}v42189/CyO

Bloomington

12706

w¹¹¹⁸; P{GT1}ics^BG02577

Genomic Clones ( 2 )

cDNA Clones ( 39 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0028546

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0028546

Antibody Information

Other Information

Discoverer

Etymology

Named "icarus" after the son of Daedalus who had unstable wings.

(Kadrmas et al., 2004)

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for identity of: BG:BACR48E02.4 CG9031

(Guan, 1999.11)

Source for identity of: Rsu-1 CG9031

(Smith et al., 2005)

Source for identity of: ics Rsu-1

(Kadrmas et al., 2004)

Source for database merge of

Additional comments

Comments About Role

Comments About Molecular Function

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_135821

RefSeq (Proteins)

•

NP_609665

DDBJ / EMBL / GenBank

•

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Leucine-rich repeat (IPR001611)

Leucine-rich repeat, typical subtype (IPR003591)

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

60808

DEDB - Drosophila exon database: splicing graphs

•

7548

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0028546

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0028546

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0028546

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0028546

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0028546

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000012358
Anopheles gambiae (Ensembl)
ENSBTAP00000027620
Bos taurus (Ensembl)
ENSCAFP00000006853
Canis familiaris (Ensembl)
ENSCINP00000016323
Ciona intestinalis (Ensembl)
NEWSINFRUP00000136160
Takifugu rubripes (Ensembl)
ENSGALP00000014173
Gallus gallus (Ensembl)
ENSP00000343559
Homo sapiens (Ensembl)
ENSMMUP00000021140
Macaca mulatta (Ensembl)
ENSMODP00000012449
Monodelphis domestica (Ensembl)
ENSPTRP00000003935
Pan troglodytes (Ensembl)
ENSRNOP00000024086
Rattus norvegicus (Ensembl)
GSTENP00009444001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000016230
Xenopus tropicalis (Ensembl)
WBGene00031312
Caenorhabditis briggsae (Wormbase)
WBGene00007924
Caenorhabditis elegans (Wormbase)
DDB0183955
Dictyostelium discoideum (Dictybase)
MGI:103040
Mus musculus (Informatics)

PANTHER - Protein classification by function, families, and pathways

•

FBgn0028546

University of Chicago Dev. Expression

•

FBgn0028546

Synonyms & Secondary IDs ( 9 )

Reported As

Symbol Synonym

BG:BACR48E02.4

(Bolshakov et al., 2002, Ashburner et al., 1999.10.12, Ashburner et al., 1999, Ashburner et al., 1999, Ashburner et al., 1999, Giot et al., 2003, )

CG9031

(Kadrmas et al., 2004)

CT25926

(Hynes and Zhao, 2000)

ics

(Kadrmas et al., 2004)

RSU-1

(Smith et al., 2005, Kadrmas et al., 2004, Kadrmas et al., 2004, Elias et al., 2008)

Rsu-1

(Girard et al., 2006)

Name Synonym

icarus

(Kadrmas et al., 2004)

Ras suppressor-1

(Smith et al., 2005)

Ras Suppressor-1

(Elias et al., 2008)

Secondary FlyBase IDs

References ( 20 )

Research paper

Girard et al., 2006, Dev. Biol. 299(2): 530--542: Chromatin immunoprecipitation reveals a novel role for the Drosophila SoxNeuro transcription factor in axonal patterning. [FBrf0194303]
Kadrmas et al., 2004, J. Cell Biol. 167(6): 1019--1024: The integrin effector PINCH regulates JNK activity and epithelial migration in concert with Ras suppressor 1. [FBrf0184059]
Bolshakov et al., 2002, Genome Res. 12(1): 57--66: A comparative genomic analysis of two distant Diptera, the fruit fly, Drosophila melanogaster, and the malaria mosquito, Anopheles gambiae. [FBrf0141561]
Ashburner et al., 1999, Genetics 153(1): 179--219: An exploration of the sequence of a 2.9-Mb region of the genome of Drosophila melanogaster. The Adh region. [FBrf0111326]

Supplementary material

Kadrmas et al., 2004, J. Cell Biol. 167(6):: Figure S1 (Cuticles of embryos) and Figure S2 (Plasmids for expression of His-tagged PINCH or RSU-1). [FBrf0199184]
Giot et al., 2003, Science 302(5651):: Tables S1 to S7. [FBrf0166452]
Hynes and Zhao, 2000, J. Cell Biol. 150(2):: Table S7. LRR repeat-containing proteins. [FBrf0132111]
Ashburner et al., 1999, Genetics 153(1):: Table S3. A classified summary of the inferred function of the products of 91 genes in the Adh region. [FBrf0112152]
Ashburner et al., 1999, Genetics 153(1):: Table S2. Genes, known and predicted, that have been determined on the sequence of the Adh region. All genes are protein coding, unless indicated tscan in the prediction column, these are tRNAs. [FBrf0112151]

Personal communication to FlyBase

Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms.: Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]
Ryder, 2004.4.26, Exelixis CG deletion data.