Gene Dmel\Taf10

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General Information

Symbol

Dmel\Taf10

Species

D. melanogaster

Name

TBP-associated factor 10

Annotation symbol

CG2859

Feature type

protein_coding_gene

FlyBase ID

FBgn0028398

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

23A5-23A5

Sequence location

2L:2,767,912..2,768,613 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene TBP-associated factor 10 is referred to in FlyBase by the symbol Taf10 (CG2859, FBgn0028398). It has the cytological map location 23A5. Its sequence location is 2L:2767912..2768613. Its molecular function is described as: RNA polymerase II transcription factor activity; general RNA polymerase II transcription factor activity. It is involved in the biological process transcription initiation from RNA polymerase II promoter. 2 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

23A5-23A5

Limits computationally determined from genome sequence between P{EP}EP832 and P{PZ}oho23B⁰³⁵⁷⁵

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

23A-23A

(Tora, 1999.7.13)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Overall orientation not stated: colt+ Taf10- colt+ Taf10b+ Taf10- Taf10b+ anon-23Ac-

(Georgieva et al., 2000)

Gene Model & Products

Please see the GBrowse view of Dmel\Taf10 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Taf10-RA

FBtr0077717

NM_058115

643

167

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Taf10-PA

FBpp0077401

18.5

167

4.25

NP_477463

AAF51210

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0028398

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Transcription initiation factor TFIID, 23-30 kDa subunit (IPR003923)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9U5W9

Maps to

DS08923

(FlyBase, 1992-)

Does NOT map to

Identified with

RE73934

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

5543

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0028398

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0028398

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0028398

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of Taf10	Class	Mutagen	Stocks	Known lesion
Taf10^KG07031		P-element activity	1	--

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Taf10	Class	Mutagen	Stocks	Known lesion
Taf10^GD4727		in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes

Aneuploid Aberrations

Useful deficiency

Df(2L)C144

Disrupted in

Df(2L)Exel6277

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD4727}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{SUPor-P}Taf10^KG07031

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 6 )

Molecular Function

CV term

Evidence

References

RNA polymerase II transcription factor activity

inferred from direct assay

(Georgieva et al., 2000)

general RNA polymerase II transcription factor activity

inferred from sequence or structural similarity with HGNC_gene:TAF10; HGNC:11543; OMIM:600475 AND inferred from sequence or structural similarity with SGD_LOCUS:TAF25; SGD:S0002574

(Aoyagi and Wassarman, 2000)

Biological Process

CV term

Evidence

References

transcription initiation from RNA polymerase II promoter

inferred from direct assay

(Georgieva et al., 2000)

inferred from sequence or structural similarity with HGNC_gene:TAF10; HGNC:11543; OMIM:600475 AND inferred from sequence or structural similarity with SGD_LOCUS:TAF25; SGD:S0002574

(Aoyagi and Wassarman, 2000)

Cellular Component

CV term

Evidence

References

SAGA complex

inferred from physical interaction with FLYBASE:Ada2b; FB:FBgn0037555 AND inferred from physical interaction with FLYBASE:Spt3; FB:FBgn0037981 AND inferred from physical interaction with FLYBASE:dik; FB:FBgn0030891

(Kusch et al., 2003)

histone acetyltransferase complex

inferred from sequence or structural similarity with HGNC_gene:TAF10; HGNC:11543; OMIM:600475 AND inferred from sequence or structural similarity with SGD_LOCUS:TAF25; SGD:S0002574

(Aoyagi and Wassarman, 2000)

transcription factor TFIID complex

inferred from direct assay

(Georgieva et al., 2000)

inferred from sequence or structural similarity with HGNC_gene:TAF10; HGNC:11543; OMIM:600475 AND inferred from sequence or structural similarity with SGD_LOCUS:TAF25; SGD:S0002574

(Aoyagi and Wassarman, 2000)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Taf10 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

59692

Orthologs

Genome-wide drosophilid orthologs

Dana\GF13893

Dere\GG24883

Dgri\GH11693

Dmoj\GI17138

Dper\GL26583

Dpse\GA28988

Dsec\GM18365

Dsim\GD23181

Dvir\GJ16180

Dwil\GK24581

Dyak\GE18180

Curated drosophilid orthologs

Dpse\GA15489

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000013303
Anopheles gambiae (Ensembl)
AT4G31720.1
Arabidopsis thaliana (Arabidopsis)
WBGene00030525
Caenorhabditis briggsae (Wormbase)
WBGene00006392
Caenorhabditis elegans (Wormbase)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

VDRC

v15334

w¹¹¹⁸; P{GD4727}v15334

v15333

w¹¹¹⁸; P{GD4727}v15333/TM3

Bloomington

14067

y¹ w^67c23; P{SUPor-P}Taf10^KG07031

Genomic Clones ( 1 )

BACR42J13

cDNA Clones ( 15 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones