Gene Dmel\hug

Help

Close All Open All

General Information

Symbol

Dmel\hug

Species

D. melanogaster

Name

hugin

Annotation symbol

CG6371

Feature type

protein_coding_gene

FlyBase ID

FBgn0028374

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

87B15-87B15

Sequence location

3R:8,351,910..8,354,798 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene hugin is referred to in FlyBase by the symbol hug (CG6371, FBgn0028374). It has the cytological map location 87B15. Its sequence location is 3R:8351910..8354798. Its molecular function is described as: neurotrophin receptor binding; hormone activity; neuropeptide hormone activity; receptor binding. It is involved in the biological processes: ecdysis, chitin-based cuticle; neuropeptide signaling pathway; larval feeding behavior. 4 alleles are reported. The phenotype of these alleles is annotated with second instar larva. It has one annotated transcript and one annotated polypeptide.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

87B15-87B15

Limits computationally determined from genome sequence between P{PZ}svp⁰⁷⁸⁴² and P{lacW}Vha55^j2E9

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

87C1-87C4

(Roos, 1999.10.11)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\hug for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

hug-RA

FBtr0082639

NM_079605

1033

191

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

hug-PA

FBpp0082108

20.6

191

8.47

NP_524329

AAF54833

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Pyrokinin, conserved site (IPR001484)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9VG55

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

GH03388

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

9224

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI063524

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0028374

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0028374

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0028374

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | partially, with Scer\GAL4^αTub84B.PL

hug^Scer\UAS.cMa

lethal | partially, with Scer\GAL4^105.1

hug^Scer\UAS.cMa

lethal | larval stage, with Scer\GAL4^αTub84B.PL

hug^Scer\UAS.cMa

Other Phenotypes

Allele

small body | larval stage, with Scer\GAL4^αTub84B.PL

hug^Scer\UAS.cMa

feeding behavior defective | larval stage, with Scer\GAL4^αTub84B.PL

hug^Scer\UAS.cMa

Phenotype manifest in

Allele

second instar larva, with Scer\GAL4^αTub84B.PL

hug^Scer\UAS.cMa

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of hug	Class	Mutagen	Stocks	Known lesion
hug^MB02782			1	--

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of hug	Mutagen	Stocks	Known lesion
hug^GD12474	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
hug^{MtnA.T:SV5\V5,T:Zzzz\His6}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	--
hug^Scer\UAS.cMa	in vitro construct \| regulatory fusion	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(3R)kar-Sz12

Useful duplication

Dp(3;3)D1a

Disrupted in

Df(3R)Exel7317

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD12474}

P{UAS-hug.M}

Insertions

Type of insertions

Name

Expression data

insertion of enhancer trap binary system

Mi{ET1}hug^MB02782

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 8 )

Molecular Function

CV term

Evidence

References

hormone activity

non-traceable author statement

(Hewes and Taghert, 2001)

neuropeptide hormone activity

non-traceable author statement

(Meng et al., 2002)

non-traceable author statement

(Baggerman et al., 2002)

inferred from electronic annotation with InterPro:IPR001484

(FlyBase Curators et al., 2004-)

neurotrophin receptor binding

non-traceable author statement

(Roos, 1999.10.11)

receptor binding

inferred from sequence or structural similarity

(Park et al., 2002)

Biological Process

CV term

Evidence

References

ecdysis, chitin-based cuticle

inferred from mutant phenotype

(Meng et al., 2002)

larval feeding behavior

inferred from mutant phenotype

(Melcher and Pankratz, 2005)

neuropeptide signaling pathway

non-traceable author statement

(Baggerman et al., 2002)

inferred from electronic annotation with InterPro:IPR001484

(FlyBase Curators et al., 2004-)

Cellular Component

CV term

Evidence

References

cellular_component

no biological data available

(FlyBase Curators, 2002-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

hug allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

66642

Orthologs

Genome-wide drosophilid orthologs

Dana\GF23232

Dere\GG19060

Dgri\GH18699

Dmoj\GI24467

Dper\GL13916

Dpse\GA19546

Dsec\GM24044

Dsim\GD18843

Dvir\GJ10559

Dwil\GK13719

Dyak\GE26204

Curated drosophilid orthologs

Dpse\GA19546

Linkouts

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

VDRC

v26765

w¹¹¹⁸; P{GD12474}v26765

v26766

w¹¹¹⁸; P{GD12474}v26766

Bloomington

23491

w¹¹¹⁸; Mi{ET1}hug^MB02782

Genomic Clones ( 1 )

BACR02G11

cDNA Clones ( 31 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

GH03388

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

EK197021

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0028374

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0028374

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: hug CG6371

(Roos, 2000.4.18)

Source for merge of: hug CG6371 Source for merge of hug CG6371 was sequence comparison (date:000423).

(FlyBase, 1996-)

Additional comments

Comments About Role

hug codes for neuropeptides with myostimulatory and ecdysis modifying effects.

(Meng et al., 2002)

Comments About Molecular Function

hug has coding capacity for at least two neuropeptides: Drm-PK-2 and hugγ.

(Meng et al., 2002)

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_079605

RefSeq (Proteins)

•

NP_524329

DDBJ / EMBL / GenBank

•

UniProtKB/Swiss-Prot

•

Q9VG55

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Pyrokinin, conserved site (IPR001484)

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

66642

DEDB - Drosophila exon database: splicing graphs

•

9224

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0028374

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0028374

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0028374

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0028374

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0028374

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0028374

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

•

/dbzhnsky/hugin1.htm

University of Chicago Dev. Expression

•

FBgn0028374

Synonyms & Secondary IDs ( 15 )

Reported As

Symbol Synonym

CG6371

(Choi et al., 2001, Hewes and Taghert, 2001, Roos, 2000.4.18, )

Drm-MT2

(Baggerman et al., 2002)

Drm-PK-2

(Rosenkilde et al., 2003, Park et al., 2003, Park et al., 2002, Meng et al., 2002, Cazzamali et al., 2005)

hug

(Anonymous, 2005, Melcher and Pankratz, 2005)

hug-γ

(Rosenkilde et al., 2003, Egerod et al., 2003, Egerod et al., 2003)

HUGγ

(Park et al., 2003)

hug γ

(Iversen et al., 2002, Iversen et al., 2002)

hugγ

(Park et al., 2002, Meng et al., 2002)

Hug-γ

(Cazzamali et al., 2005)

pyrokinin/PBAN-like

(Choi et al., 2001)

Name Synonym

hugin

(Meng et al., 1999, Peters and Pankratz, 2007, Bader and Pankratz, 2007, Anonymous, 2005, Melcher et al., 2006, Melcher and Pankratz, 2005)

Hugin-γ

(Yapici et al., 2008)

pyrokinin

(Cazzamali et al., 2003)

pyrokinin-2

(Rosenkilde et al., 2003, Cazzamali et al., 2005, Verleyen et al., 2004)

SVPFKPRLamide

(Rosenkilde et al., 2003)

Secondary FlyBase IDs

FBgn0038062

References ( 35 )

Generate a list of

All references relating to this gene ( 35 )

List References by type

Recent research papers ( 2 )

Yapici et al., 2008, Nature 451(7174): 33--37: A receptor that mediates the post-mating switch in Drosophila reproductive behaviour. [FBrf0202765]
Melcher et al., 2006, PLoS Biol. 4(3): 316: Neuromedin U and its putative Drosophila homolog hugin. [FBrf0191371]

Show all research papers ...

Recent reviews (0)

All reviews listed in FlyBase were published before 2006

Show reviews ...