Gene Dmel\Akap200

General Information
Symbol	Dmel\Akap200	Species	D. melanogaster
Name	A kinase anchor protein 200	Annotation symbol	CG13388
Feature type	protein_coding_gene	FlyBase ID	FBgn0027932
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	29C3-29C4	Sequence location	2L:8,415,690..8,430,984 [+]
Map ( GBrowse )
Summary Information
Automatically generated summary See sections below for more information	The gene A kinase anchor protein 200 is referred to in FlyBase by the symbol Akap200 (CG13388, FBgn0027932). It has the cytological map location 29C3-29C4. Its sequence location is 2L:8415690..8430984. Its molecular function is described as protein kinase A binding. It is involved in the biological processes: protein localization; negative regulation of Ras protein signal transduction; autophagic cell death; salivary gland cell autophagic cell death; type I hypersensitivity. 20 alleles are reported. The phenotypes of these alleles are annotated with: wing; inner nurse cell ring canal rim; outer nurse cell ring canal rim; nurse cell; egg chamber. It has 4 annotated transcripts and 4 annotated polypeptides.
External Summaries

Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 29C3-29C4 Limits computationally determined from genome sequence between P{lacW}l(2)k12914^k12914&P{lacW}Akap200^k07118a and P{lacW}l(2)k03706^k03706&P{lacW}Acer^k07704
Experimentally Determined Cytological Location
Cytogenetic map Notes References 29C1-29C2 (determined by in situ hybridisation) (Huang and Rubin, 2000) 29C3-29C3 (Rubin, 1999.3.3)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References Overall orientation not stated: fy? Akap200? grk? (Li et al., 1999)
Gene Model & Products
Please see the GBrowse view of Dmel\Akap200 for information on other features
Comments on Gene Model
	DGC clone LP02734 appears problematic: incomplete CDS
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Akap200-RA FBtr0079664 NM_058112 3113 753 Akap200-RB FBtr0079666 NM_058111 1874 372 Akap200-RC FBtr0079665 NM_164820 1957 372 Akap200-RD FBtr0079667 NM_175984 3358 837
Additional Transcript Data & Comments
Reported size (kB)	3.053, 1.119 (longest cDNA) (Li et al., 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Akap200-PA FBpp0079279 79.0 753 3.80 NP_477460 AAF52674 Akap200-PB FBpp0079281 38.2 372 4.18 NP_477459 AAN10667 Akap200-PC FBpp0079280 38.2 372 4.18 NP_723395 AAN10668 Akap200-PD FBpp0079282 88.1 837 3.86 NP_787998 AAO41167
Additional Polypeptide Data & Comments
Reported size (kD)	753, 372 (aa); 200, 95 (kD observed) (Li et al., 1999)
Comments	Their characteristics indicate that the small (95 kD) Akap200 protein is a member of the myristoylated alanine-rich C kinase substrate protein family, whereas the large (200 kD) Akap200 protein is a chimeric A kinase anchor protein-myristoylated alanine-rich C kinase substrate protein. Both large and small Akap200 proteins are myristoylated, but are not palmitoylated. N-terminal myristoylation correlates with the intracellular targeting of the 200 kD Akap200 protein: epitope-tagged 200 kD Akap200 protein is targeted to the surface of S2 cells, while epitope-tagged Akap200 protein missing the first 7 amino acids is distributed throughout the cytoplasm. The PSD (phosphorylation site domain) at amino acids 119-148 of both Akap200 proteins is able to bind with F-actin and calmodulin; this domain can be phosphorylated in vitro by protein kinase C, and phosphorylation interferes with the interaction of the PSD with F-actin and calmodulin. The Pka-R2 tethering domain of the 200 kD Akap200 protein is located at amino acid residues 511-531. (Rossi et al., 1999, Rossi et al., 1999)
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Pollen allergen Poa pIX/Phl pVI, C-terminal (IPR001778)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201626 AA202180 AA202468 AA202492 AA202932 AA203033 AA246411 AA247023 AA263604 AA264350 AA390996 AA391108 AA391712 AA391985 AA392410 AA392477 AA392796 AA438770 AA439185 AA439410 AA538635 AA539975 AA540046 AA540110 AA540541 AA541057 AA541193 AA567300 AA567899 AA697381 AA735607 AA803038 AA803082 AA816448 AA816730 AA820057 AA820668 AA941131 AA941313 AA941371 AA941565 AA941668 AA942497 AA949329 AA950814 AA950926 AA951517 AA951601 AA978549 AA978815 AA978910 AA979008 AA990629 AC004423 AE003621 AAF52674 AE003621 AAN10667 AE003621 AAN10668 AE003621 AAO41167 AF132884 AAD47200 AF132885 AAD47201 AI107627 AI114059 AI114381 AI134692 AI135781 AI238256 AI238342 AI239219 AI258172 AI258601 AI258963 AI259342 AI260058 AI260912 AI297128 AI297581 AI404367 AI455185 AI456600 AI456845 AI511900 AI512803 AI512852 AI513867 AI514280 AI515000 AI515300 AI515819 AI516193 AI516508 AI516792 AI516982 AI517834 AI518345 AI519718 AI532326 AI533406 AI533655 AI534328 AI534538 AI534555 AI542085 AI542264 AI542381 AI542390 AI543951 AQ025816 AQ073434 AQ073691 AW943629 AW943999 AX094010 AX094089 AY075431 AAL68246 AY075431 AAO24995 BF486768 BF487023 BF487873 BF491505 BF491685 BF493970 BF496708 BF498387 BF499618 BF502571 BF502747 BF504630 BF505098 BF505143 BG633830 BG633909 BG635499 BG635761 BG636740 BG637344 BG638690 BG639915 BG640450 BG640534 BG640685 BI141767 BI141784 BI162080 BI162081 BI165199 BI165611 BI166128 BI169895 BI170592 BI171324 BI173023 BI216626 BI232825 BI234060 BI234198 BI234421 BI235544 BI235662 BI235940 BI236024 BI239890 BI240073 BI242310 BI242621 BI351760 BI354878 BI356804 BI365586 BI368188 BI368368 BI371492 BI372975 BI374265 BI375251 BI482096 BI482617 BI483820 BI486909 BI577219 BI620617 BI630000 BI635099 BI635618 BI635843 BI635904 BI636105 BI636315 BI637965 BI640189 BI640574 BI640989 BP547972 BP551377 BP551378 BP554122 BP554123 BP556976 BP556977 BP558494 BP558495 BQ103388 BQ103623