Gene Dmel\htt

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General Information

Symbol

Dmel\htt

Species

D. melanogaster

Name

huntingtin

Annotation symbol

CG9995

Feature type

protein_coding_gene

FlyBase ID

FBgn0027655

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

98E2-98E2

Sequence location

3R:24,521,964..24,560,073 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene huntingtin is referred to in FlyBase by the symbol htt (CG9995, FBgn0027655). It has the cytological map location 98E2. Its sequence location is 3R:24521964..24560073. Its molecular function is described as: microtubule binding; protein binding; binding. It is involved in the biological process axon cargo transport. 9 alleles are reported. The phenotypes of these alleles are annotated with: larval brain; eye; ommatidium. It has one annotated transcript and one annotated polypeptide.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

98E2-98E2

Limits computationally determined from genome sequence between P{PZ}l(3)06487⁰⁶⁴⁸⁷ and P{EP}EP3390^EP3390

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

98E-98E

(Takano, 1999.8.12)

98E1-98E2

(Li, 1999.4.27)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\htt for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

htt-RA

FBtr0085310

NM_143372

11579

3583

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

htt-PA

FBpp0084679

395.9

3583

6.01

NP_651629

AAF56808

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0027655

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

HEAT (IPR000357)

Huntingtin (IPR000091)

Armadillo-type fold (IPR016024)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

DS04556

(Li et al., 1999)

Does NOT map to

Identified with

LD23533

(Li et al., 1999, BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

11130

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0027655

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0027655

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0027655

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

htt^c07030

Sterility

Allele

fertile

htt^c07030

Phenotype manifest in

Allele

axon & larva, with Scer\GAL4^Appl.G1a

htt^{dsRNA.Scer\UAS}

larval brain, with Scer\GAL4^Appl.G1a

htt^{dsRNA.Scer\UAS}

eye, with Scer\GAL4^GMR.PS

htt^{dsRNA.Scer\UAS}

ommatidium, with Scer\GAL4^GMR.PS

htt^{dsRNA.Scer\UAS}

Classical Alleles ( 6 )

For All Classical Alleles Show

Allele of htt	Mutagen	Stocks	Known lesion
htt^MB03997		1	--
htt^c00389	piggyBac transposase	1	--
htt^c05407	piggyBac transposase	0	--
htt^c07030	piggyBac transposase	1	--
htt^f04684	piggyBac transposase	1	--
htt^f05417	piggyBac transposase	1	--

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of htt	Mutagen	Stocks	Known lesion
htt^GD14339	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
htt^GD14943	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
htt^{dsRNA.Scer\UAS}	in vitro construct \| regulatory fusion in vitro construct \| RNAi	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(3R)RR38

Useful duplication

Dp(3;3)501

Disrupted in

Df(3R)Exel6210

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD14339}

P{GD14943}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{PB}huntingtin^c00389

PBac{PB}huntingtin^c05407

PBac{PB}huntingtin^c07030

PBac{WH}huntingtin^f04684

PBac{WH}huntingtin^f05417

insertion of enhancer trap binary system

Mi{ET1}htt^MB03997

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 6 )

Molecular Function

CV term

Evidence

References

binding

inferred from electronic annotation with InterPro:IPR016024

(FlyBase Curators et al., 2004-)

microtubule binding

non-traceable author statement

(Gunawardena and Goldstein, 2002)

protein binding

non-traceable author statement

(Gunawardena and Goldstein, 2002)

Biological Process

CV term

Evidence

References

axon cargo transport

non-traceable author statement

(Gunawardena and Goldstein, 2002)

Cellular Component

CV term

Evidence

References

cytoplasm

non-traceable author statement

(Gunawardena and Goldstein, 2002)

inferred from electronic annotation with InterPro:IPR000091

(FlyBase Curators et al., 2004-)

nucleus

inferred from electronic annotation with InterPro:IPR000091

(FlyBase Curators et al., 2004-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

htt allele

Gene

References

htt^{dsRNA.Scer\UAS}

Klc

(Gunawardena et al., 2004)

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

68268

Drosophila PIMRider - The Drosophila Protein Interaction map

•

521599

Orthologs

Genome-wide drosophilid orthologs

Dana\GF18586

Dere\GG11611

Dgri\GH14159

Dmoj\GI22240

Dper\GL13796

Dpse\GA26800

Dsec\GM12734

Dvir\GJ23045

Dwil\GK12103

Dyak\GE23798

Curated drosophilid orthologs

Dpse\GA22175

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000020200
Anopheles gambiae (Ensembl)
NEWSINFRUP00000153960
Takifugu rubripes (Ensembl)
ENSGALP00000025163
Gallus gallus (Ensembl)
ENSMODP00000004422
Monodelphis domestica (Ensembl)
GSTENP00022637001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000032279
Xenopus tropicalis (Ensembl)
ZDB-GENE-990415-131
Danio rerio (Zfin)
MGI:96067
Mus musculus (Informatics)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 10 )

v29532

w¹¹¹⁸; P{GD14943}v29532

v36204

w¹¹¹⁸; P{GD14339}v36204

v29531

w¹¹¹⁸; P{GD14943}v29531/TM3

v36205

w¹¹¹⁸; P{GD14339}v36205/TM3

Bloomington

24665

w¹¹¹⁸; Mi{ET1}htt^MB03997

17797

w¹¹¹⁸; PBac{PB}huntingtin^c07030

Genomic Clones ( 1 )

BACR10P23

cDNA Clones ( 10 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

LD23533

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0027655

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0027655

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Comments About Role

Comments About Molecular Function

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_143372

RefSeq (Proteins)

•

NP_651629

DDBJ / EMBL / GenBank

•

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

HEAT (IPR000357)

Huntingtin (IPR000091)

Armadillo-type fold (IPR016024)

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

68268

DEDB - Drosophila exon database: splicing graphs

•

11130

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0027655

Drosophila PIMRider - The Drosophila Protein Interaction map

•

521599

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0027655

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0027655

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0027655

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0027655

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0027655

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000020200
Anopheles gambiae (Ensembl)
NEWSINFRUP00000153960
Takifugu rubripes (Ensembl)
ENSGALP00000025163
Gallus gallus (Ensembl)
ENSMODP00000004422
Monodelphis domestica (Ensembl)
GSTENP00022637001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000032279
Xenopus tropicalis (Ensembl)
ZDB-GENE-990415-131
Danio rerio (Zfin)
MGI:96067
Mus musculus (Informatics)

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

•

/hjmuller/huntingtin1.htm

PANTHER - Protein classification by function, families, and pathways

•

FBgn0027655

Synonyms & Secondary IDs ( 8 )

Reported As

Symbol Synonym

CG9995

dhtt

(Gunawardena et al., 2004)

(Li et al., 1999)

Hsap\HD

(Li, 1999.5.1, Li, 1999.4.27)

Htt

(Driscoll and Gerstbrein, 2003)

htt

HTT

(Lin and Beal, 2006)

Name Synonym

huntingtin

(Hill, 2006, Cha et al., 2008, Stark et al., 2007)

Secondary FlyBase IDs

References ( 31 )

Generate a list of

All references relating to this gene ( 31 )

List References by type

Recent research papers ( 1 )

Stark et al., 2007, Nature 450(7167): 219--232: Discovery of functional elements in 12 Drosophila genomes using evolutionary signatures. [FBrf0200674]

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Recent reviews ( 2 )

Hill, 2006, PLoS Biol. 4(12): e446: Cellular inheritance. [FBrf0200122]
Lin and Beal, 2006, Nature 443(7113): 787--795: Mitochondrial dysfunction and oxidative stress in neurodegenerative diseases. [FBrf0200017]

Show all reviews ...