Gene Dmel\Acf1

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Acf1-PA

FBpp0085204

170.4

1476

6.86

NP_536734

AAF57200

Additional Polypeptide Data & Comments

Reported size (kD)

1476 (aa); 185, 170 (kD observed); 170 (kD predicted)

Comments

External Data

(BDGP Project Members, 2000-)

PANTHER - Protein classification by function, families, and pathways

•

FBgn0027620

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Bromodomain (IPR001487)

Zinc finger, PHD-type (IPR001965)

DDT (IPR004022)

Zinc finger, FYVE/PHD-type (IPR011011)

WSTF/Acf1/Cbp146 (IPR013136)

Zinc finger, RING/FYVE/PHD-type (IPR013083)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

DEDB - Drosophila exon database: splicing graphs

•

11489

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

embryonic stage-adult stage

Marker for

Subcellular Localization

CV Term

External Data & Images

Show all BDGP original images, annotations and embryonic microarray data for Acf1

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0027620

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0027620

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0027620

BDGP expression data

•

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for Acf1

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable | recessive

semi-lethal | recessive

Other Phenotypes

Allele

suppressor of variegation | recessive, with In(1)w^m4h

Acf1¹

suppressor of variegation | recessive, with P{SUPor-P}KV0123

Acf1¹

suppressor of variegation | recessive, with P{2212H6.5+PBX0.6}2L

Acf1¹

non-suppressor of variegation | recessive, with P{hsp26-pt-T}39C-5

Acf1¹

non-suppressor of variegation, with In(1)w^m4h

Acf1³

non-suppressor of variegation, with P{SUPor-P}KV0123

Acf1³

mitotic cell cycle defective | recessive

Acf1¹

developmental rate defective | recessive

Acf1¹

Sterility

Allele

fertile | recessive

Phenotype manifest in

Allele

S phase

Acf1¹

Classical Alleles ( 11 )

For All Classical Alleles Show

Allele of Acf1	Mutagen	Stocks	Known lesion
Acf1¹	P-element activity	0	Yes
Acf1²	P-element activity	0	Yes
Acf1³	P-element activity	0	Yes
Acf1⁴	P-element activity	0	Yes
Acf1^EP1181	P-element activity	1	--
Acf1^EY08629		1	--
Acf1^KG06618b	P-element activity	1	--
Acf1^c01552	piggyBac transposase	1	--
Acf1^c01856	piggyBac transposase	1	--
Acf1^c05751	piggyBac transposase	1	--
Acf1^e01399	piggyBac transposase	1	--

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Acf1	Class	Mutagen	Stocks	Known lesion
Acf1^GD9690		in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes

Aneuploid Aberrations

Useful deficiency

Df(3R)R113

Useful duplication

Dp(3;3)516

Disrupted in

Df(3R)Exel6220

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD9690}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{SUPor-P}Acf1^KG06618b

insertion of mobile activating element

P{EPgy2}Acf1^EY08629

P{EP}Acf1^EP1181

Related Comments

Please look at the allele reports for the complete phenotype data

dsRNA has been made from templates generated with primers directed against this gene. RNAi of Acf1 causes an increase in the number of class I da neurons. RNAi also causes defects in muscle, defects in the epidermis, alterations in the number of MD neurons and reproducible defects in da dendrite development.

(Lusser and Kadonaga, 2003)

Gene Ontology: Function, Process & Cellular Component ( 17 )

Molecular Function

CV term

Evidence

References

DNA binding

traceable author statement

NOT
histone acetyltransferase activity

inferred from direct assay

(FlyBase Curators et al., 2004-)

protein binding

inferred from electronic annotation with InterPro:IPR001965

zinc ion binding

inferred from electronic annotation with InterPro:IPR001965, InterPro:IPR011011

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

chromatin assembly or disassembly

non-traceable author statement

(Ito, 1999.5.3)

non-traceable author statement

(FlyBase, 1992-)

traceable author statement

(Lusser and Kadonaga, 2003)

dendrite morphogenesis

inferred from mutant phenotype

epidermis development

inferred from mutant phenotype

muscle development

inferred from mutant phenotype

neuron development

inferred from mutant phenotype

nucleosome assembly

inferred from direct assay

inferred from direct assay

(Eberharter et al., 2001)

nucleosome mobilization

inferred from direct assay

traceable author statement

(Lusser and Kadonaga, 2003)

nucleosome positioning

inferred from direct assay

regulation of transcription, DNA-dependent

inferred from direct assay

Cellular Component

CV term

Evidence

References

ACF complex

inferred from direct assay

inferred from direct assay inferred from physical interaction with FLYBASE:Iswi; FB:FBgn0011604

NOT
NURF complex

inferred from direct assay

chromatin accessibility complex

non-traceable author statement

(Ferrari, 1999.4.20)

inferred from direct assay

(Eberharter et al., 2001)

chromatin assembly complex

non-traceable author statement

(Ito, 1999.5.3)

non-traceable author statement

(FlyBase, 1992-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Acf1 allele

Gene

References

Acf1¹

Nap1

(Fyodorov et al., 2004)

External Data

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

68592

Drosophila PIMRider - The Drosophila Protein Interaction map

•

522247

Orthologs

Genome-wide drosophilid orthologs

Dana\GF17489

Dere\GG11833

Dgri\GH13739

Dmoj\GI24812

Dper\GL24024

Dpse\GA15159

Dsec\GM16453

Dsim\GD21605

Dvir\GJ24457

Dwil\GK12157

Dyak\GE10967

Curated drosophilid orthologs

Dpse\GA15159

y¹ w^67c23; P{EPgy2}Acf1^EY08629

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000001532
Anopheles gambiae (Ensembl)
ENSBTAP00000026857
Bos taurus (Ensembl)
ENSCAFP00000019619
Canis familiaris (Ensembl)
NEWSINFRUP00000148294
Takifugu rubripes (Ensembl)
ENSGALP00000016270
Gallus gallus (Ensembl)
ENSP00000353458
Homo sapiens (Ensembl)
ENSMMUP00000019759
Macaca mulatta (Ensembl)
ENSMODP00000016198
Monodelphis domestica (Ensembl)
ENSPTRP00000010620
Pan troglodytes (Ensembl)
ENSRNOP00000009080
Rattus norvegicus (Ensembl)
GSTENP00003597001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000026890
Xenopus tropicalis (Ensembl)
WBGene00019217
Caenorhabditis elegans (Wormbase)
ZDB-GENE-030131-6105
Danio rerio (Zfin)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 9 )

v33446

w¹¹¹⁸ P{GD9690}v33446

v33447

w¹¹¹⁸; P{GD9690}v33447

Bloomington

16432

14307

y¹ w^67c23; P{SUPor-P}KG06618a P{SUPor-P}Acf1^KG06618b ry⁵⁰⁶

Genomic Clones ( 2 )

cDNA Clones ( 14 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0027620

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0027620

Antibody Information

polyclonal

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for identity of: Acf1 CG1966

(Zhao, 1999.11)

Source for database merge of

Additional comments

Comments About Role

Acf1, a multisubunit factor consisting of polypeptides with molecular masses of 47, 140, 170 and 185kD, has been purified and characterised. The 140kD component is encoded by Iswi. Acf1 is distinct from NURF, a nucleosome remodelling factor that also contains Iswi. Acf1 together with a core histone chaperone (such as Caf1 or Nap1) is sufficient for ATP-dependent formation of periodic nucleosome arrays. In chromatin remodelling, Acf1 is able to modulate the internucleosomal spacing of chromatin by an ATP-dependent mechanism.

The Acf1 and Iswi subunits of ACF (ATP-utilizing chromatin assembly and remodeling factor) function synergistically in the assembly of chromatin.

Comments About Molecular Function

Other Comments

ACF (ATP-utilizing chromatin assembly and remodeling factor) is a multisubunit factor consisting of 3 polypeptides. These are encoded by Iswi and by Acf1 (this encodes both 170 and 185kD subunits). The p47 polypeptide seen previously in ACF fractions (FBrf0095281) has been found to be identical to Yp2 and appears to have been a contaminant in these earlier ACF fractions.