Gene Dmel\nito

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General Information

Symbol

Dmel\nito

Species

D. melanogaster

Name

spenito

Annotation symbol

CG2910

Feature type

protein_coding_gene

FlyBase ID

FBgn0027548

Created / Updated

2003-12-02/2003-12-02

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

43F8-43F9

Sequence location

2R:3,849,991..3,857,320 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene spenito is referred to in FlyBase by the symbol nito (CG2910, FBgn0027548). It has the cytological map location 43F8-43F9. Its sequence location is 2R:3849991..3857320. Its molecular function is described as: mRNA binding; nucleic acid binding; nucleotide binding. It is involved in the biological process regulation of transcription. 5 alleles are reported. The phenotypes of these alleles are annotated with: eye photoreceptor cell; ommatidium; rhabdomere; eye; eye disc. It has 2 annotated transcripts and 2 annotated polypeptides.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

43F8-43F9

Limits computationally determined from genome sequence between P{lacW}lin19^k01207&P{lacW}rnh1^k07624 and P{lacW}CSN4^k08018&P{lacW}l(2)k08504^k08504

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\nito for information on other features

Comments on Gene Model

DGC clone SD06722 appears problematic: incomplete CDS

DGC clone LD14125 appears problematic: incomplete CDS

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

nito-RA

FBtr0088857

NM_165579

3983

793

nito-RB

FBtr0088858

NM_136495

3102

793

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

nito-PA

FBpp0087933

89.1

793

9.71

NP_724633

AAM68878

nito-PB

FBpp0087934

89.1

793

9.71

NP_610339

AAF59160

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0027548

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

RNA recognition motif, RNP-1 (IPR000504)

Spen paralogue and orthologue C-terminal, metazoa (IPR010912)

Nucleotide-binding, alpha-beta plait (IPR012677)

Spen paralogue and orthologue C-terminal (IPR012921)

Spen Paralogue and Orthologue C-terminal-like (IPR016194)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

GH11110

(BDGP Project Members, 2000-, Jemc and Rebay, 2006)

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

13448

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI534183
AI114303

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0027548

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0027548

Alleles & Phenotypes

Summary of Allele Phenotypes

Other Phenotypes

Allele

visible, with Scer\GAL4^hs.2sev

nito^{ΔC.Scer\UAS.T:Hsap\MYC}

visible, with Scer\GAL4^sev.PR

nito^{Scer\UAS.T:Hsap\MYC}

cell death increase, with Scer\GAL4^hs.2sev

Phenotype manifest in

Allele

eye photoreceptor cell, with Scer\GAL4^sev.PR

nito^{Scer\UAS.T:Hsap\MYC}

ommatidium, with Scer\GAL4^sev.PR

nito^{Scer\UAS.T:Hsap\MYC}

rhabdomere, with Scer\GAL4^sev.PR

nito^{Scer\UAS.T:Hsap\MYC}

eye, with Scer\GAL4^sev.PR

nito^{Scer\UAS.T:Hsap\MYC}

eye | posterior, with Scer\GAL4^sev.PR

nito^{Scer\UAS.T:Hsap\MYC}

eye disc, with Scer\GAL4^hs.2sev

eye, with Scer\GAL4^hs.2sev

nito^{ΔC.Scer\UAS.T:Hsap\MYC}

ommatidium, with Scer\GAL4^hs.2sev

nito^{ΔC.Scer\UAS.T:Hsap\MYC}

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of nito	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Allele of nito	Mutagen	Stocks	Known lesion
nito^GD9850	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
nito^{Scer\UAS.T:Hsap\MYC}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes
nito^{dsRNA.Scer\UAS}	in vitro construct \| regulatory fusion in vitro construct \| RNAi	0	Yes
nito^{ΔC.Scer\UAS.T:Hsap\MYC}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes
nito^{ΔN.Scer\UAS.T:Hsap\MYC}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(2R)NCX8

Useful duplication

Dp(2;3)GYL2

Disrupted in

Df(2R)Exel6055

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

Insertions

Type of insertions

Name

Expression data

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 5 )

Molecular Function

CV term

Evidence

References

mRNA binding

inferred from sequence or structural similarity

(Lasko, 2000)

nucleic acid binding

inferred from electronic annotation with InterPro:IPR000504

(FlyBase Curators et al., 2004-)

nucleotide binding

inferred from electronic annotation with InterPro:IPR012677

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

regulation of transcription

inferred from electronic annotation with InterPro:IPR010912

(FlyBase Curators et al., 2004-)

Cellular Component

CV term

Evidence

References

nucleus

inferred from electronic annotation with InterPro:IPR010912, InterPro:IPR016194

(FlyBase Curators et al., 2004-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

nito allele

Gene

References

unspecified

spen

(Jemc and Rebay, 2006)

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

61618

Orthologs

Genome-wide drosophilid orthologs

Dana\GF13651

Dere\GG23320

Dgri\GH20604

Dmoj\GI20446

Dper\GL11478

Dpse\GA15511

Dsec\GM20997

Dsim\GD10528

Dvir\GJ20116

Dwil\GK10751

Dyak\GE19164

Curated drosophilid orthologs

Dpse\GA15511

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000009999
Anopheles gambiae (Ensembl)
ENSBTAP00000008515
Bos taurus (Ensembl)
ENSCAFP00000015065
Canis familiaris (Ensembl)
ENSCINP00000013805
Ciona intestinalis (Ensembl)
NEWSINFRUP00000178821
Takifugu rubripes (Ensembl)
ENSGALP00000003731
Gallus gallus (Ensembl)
ENSP00000313890
Homo sapiens (Ensembl)
ENSMMUP00000005727
Macaca mulatta (Ensembl)
ENSMODP00000014920
Monodelphis domestica (Ensembl)
ENSPTRP00000025846
Pan troglodytes (Ensembl)
ENSRNOP00000018994
Rattus norvegicus (Ensembl)
GSTENP00028172001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000004353
Xenopus tropicalis (Ensembl)
WBGene00024853
Caenorhabditis briggsae (Wormbase)
WBGene00017929
Caenorhabditis elegans (Wormbase)
MGI:1923598
Mus musculus (Informatics)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 1 )

VDRC

v20942

w¹¹¹⁸; P{GD9850}v20942

Genomic Clones ( 1 )

BACR26A16

cDNA Clones ( 36 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones