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Gene Dmel\CG10254

General Information
Symbol	Dmel\CG10254	Species	D. melanogaster
Name	CG10254	Annotation symbol	CG10254
Feature type	protein_coding_gene	FlyBase ID	FBgn0027512
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	95A7-95A7	Sequence location	3R:19,514,727..19,520,705 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol CG10254 (FBgn0027512). It has the cytological map location 95A7. Its sequence location is 3R:19514727..19520705. Its molecular function is described as ubiquitin-protein ligase activity. It is involved in the biological processes: regulation of protein metabolic process; post-translational protein modification. 7 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 95A7-95A7   Limits computationally determined from genome sequence between P{PZ}l(3)0690606906 and P{PZ}l(3)0468404684
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\CG10254 for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) CG10254-RA FBtr0084477  NM_142913   4927   1398 CG10254-RB FBtr0084476  NM_170081   4829   1398
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein CG10254-PA   FBpp0083868   156.6   1398   4.83     NP_651170   AAF56162 CG10254-PB   FBpp0083867   156.6   1398   4.83     NP_732888   AAN13952
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0027512
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Ubiquitin-conjugating enzyme, E2 (IPR000608) Ubiquitin-conjugating enzyme/RWD-like (IPR016135)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA438603   AA817067   AA951267   AA978376   AE003744 AAF56162   AE003744 AAN13952   AF132190 AAD34778   AI134168   AI455235   AI511711   AI533656   AT01718   BF485998   BF493390   BF493391   BF495374   BF496474   BF496847   BF498222   BF498504   BF501577   BG634397   BG639754   BI212109   BI371204   BI374784   BI482140   CK657893   CK658797   CO185507   CO286628   CO306437   CO317495   CO344352   CZ467096   CZ470922   CZ470947   CZ470948   CZ481142   CZ481143   CZ486120   CZ486641   EC065560   EC069506   EC215462   EC216044   EC221911   EC228881   EC258566   SD05555
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9XZ32
Maps to
Does NOT map to
Identified with	LD22087 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 10483
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AI533656
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0027512 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0027512
Alleles & Phenotypes
Summary of Allele Phenotypes
Phenotype manifest in Allele
Classical Alleles ( 6 )
For All Classical Alleles Show
Allele of CG10254 Class Mutagen Stocks Known lesion CG10254c01359 piggyBac transposase 1 -- CG10254c06687 piggyBac transposase 1 -- CG10254c06720 piggyBac transposase 0 -- CG10254e03373 piggyBac transposase 1 -- CG10254f03542 piggyBac transposase 1 -- CG10254f04092 piggyBac transposase 1 --
Alleles Carried on Transgenic Constructs ( 1 )
For All Alleles Carried on Transgenic Constructs Show
Allele of CG10254 Class Mutagen Stocks Known lesion CG10254GD6915 in vitro construct | RNAi in vitro construct | regulatory fusion 1 Yes
Aneuploid Aberrations
Useful duplication	Dp(3;3)M95A+13
Disrupted in	Df(3R)Exel6195 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD6915} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{PB}CG10254c01359 NA PBac{PB}CG10254c06687 NA PBac{PB}CG10254c06720 NA PBac{RB}CG10254e03373 NA PBac{WH}CG10254f03542 NA PBac{WH}CG10254f04092 NA
Related Comments
	Please look at the allele reports for the complete phenotype data
Gene Ontology: Function, Process & Cellular Component ( 3 )
Molecular Function
CV term Evidence References
ubiquitin-protein ligase activity inferred from sequence or structural similarity with EMBL:Y17267 (FlyBase, 1992-)
Biological Process
CV term Evidence References
post-translational protein modification inferred from electronic annotation with InterPro:IPR000608 (FlyBase Curators et al., 2004-)
regulation of protein metabolic process inferred from electronic annotation with InterPro:IPR000608 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions CG10254 allele Gene References
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 67731
Orthologs
Genome-wide drosophilid orthologs	Dana\GF18750 Dere\GG12418 Dgri\GH13926 Dmoj\GI24834 Dper\GL13836 Dpse\GA26813 Dsec\GM23551 Dsim\GD18366 Dvir\GJ24478 Dwil\GK14098 Dyak\GE23936
Curated drosophilid orthologs
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000020334 Anopheles gambiae (Ensembl) ENSBTAP00000026793 Bos taurus (Ensembl) ENSCAFP00000007577 Canis familiaris (Ensembl) ENSCINP00000011633 Ciona intestinalis (Ensembl) NEWSINFRUP00000159312 Takifugu rubripes (Ensembl) ENSGALP00000003058 Gallus gallus (Ensembl) ENSP00000323687 Homo sapiens (Ensembl) ENSMMUP00000024955 Macaca mulatta (Ensembl) ENSMODP00000011548 Monodelphis domestica (Ensembl) ENSPTRP00000016457 Pan troglodytes (Ensembl) ENSRNOP00000038444 Rattus norvegicus (Ensembl) ENSXETP00000042280 Xenopus tropicalis (Ensembl) Q5ZCB9 Oryza sativa (Gramene) Q9AWU5 Oryza sativa (Gramene) AT2G16920.1 Arabidopsis thaliana (Arabidopsis) MGI:2444266 Mus musculus (Informatics)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 6 )
Harvard	- PBac{PB}CG10254c01359 - PBac{PB}CG10254c06687 - PBac{RB}CG10254e03373 - PBac{WH}CG10254f03542 - PBac{WH}CG10254f04092
VDRC	v15992 w1118; P{GD6915}v15992
Genomic Clones ( 2 )
	BACR11E07 BACR16I18
cDNA Clones ( 27 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD22087
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LP16042 RE17129 AT10840 RE64792 LD26825 RE58051 LD12919 LD43203 LD31650 AT04210 AT10401 AT12925 RE29128 AT16948 AT30601 RE62457 AT20119 AT12596 GH11548
Other clones	AT01718 EK009827 EK268350 SD05555 EK251035 EK063410 EP19115
RNAi & Array Information
Affy Oligo
Linkouts	DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0027512 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0027512
Antibody Information
Other Information
Discoverer
Etymology
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Comments About Role
Comments About Molecular Function
Other Comments
	dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_170081 NM_142913 RefSeq (Proteins) • NP_732888 NP_651170 DDBJ / EMBL / GenBank • AA817067 AE003744 AAF56162 AAN13952 AF132190 AAD34778 AI455235 CZ467096 CZ470922 CZ470947 CZ470948 CZ481142 CZ481143 CZ486120 CZ486641 UniProtKB/TrEMBL • Q9XZ32
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Ubiquitin-conjugating enzyme, E2 (IPR000608) Ubiquitin-conjugating enzyme/RWD-like (IPR016135)
Linkouts
	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 67731 DEDB - Drosophila exon database: splicing graphs • 10483 DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0027512 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0027512 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0027512 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0027512 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0027512 InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000020334 Anopheles gambiae (Ensembl) ENSBTAP00000026793 Bos taurus (Ensembl) ENSCAFP00000007577 Canis familiaris (Ensembl) ENSCINP00000011633 Ciona intestinalis (Ensembl) NEWSINFRUP00000159312 Takifugu rubripes (Ensembl) ENSGALP00000003058 Gallus gallus (Ensembl) ENSP00000323687 Homo sapiens (Ensembl) ENSMMUP00000024955 Macaca mulatta (Ensembl) ENSMODP00000011548 Monodelphis domestica (Ensembl) ENSPTRP00000016457 Pan troglodytes (Ensembl) ENSRNOP00000038444 Rattus norvegicus (Ensembl) ENSXETP00000042280 Xenopus tropicalis (Ensembl) Q5ZCB9 Oryza sativa (Gramene) Q9AWU5 Oryza sativa (Gramene) AT2G16920.1 Arabidopsis thaliana (Arabidopsis) MGI:2444266 Mus musculus (Informatics) PANTHER - Protein classification by function, families, and pathways • FBgn0027512 University of Chicago Dev. Expression • FBgn0027512
Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	BcDNA:LD22087 (Giot et al., 2003, ) CG10254
Name Synonym	CG10254
Secondary FlyBase IDs
References ( 11 )
Supplementary material	Giot et al., 2003, Science 302(5651): Tables S1 to S7. [FBrf0166452] Kiger et al., 2003, J. Biol. 2(4): Additional data file 1: The gene identity and primer sequences for dsRNAs used in the RNAi screens. [FBrf0174231]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215] Ryder, 2004.4.26, Exelixis CG deletion data. Exelixis CG deletion data. [FBrf0184335]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. Genomic mapping of Exelixis insertion collection. [FBrf0184340] Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0125078]
Automatic genome annotation	FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.] [Annotations performed at the Celera Jamboreee.] [FBrf0126705]
Curated genome annotation	Li, 1999.11, [Annotations performed at the Celera Jamboreee.] [Annotations performed at the Celera Jamboreee.] [FBrf0126682]