Gene Dmel\bgm

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General Information

Symbol

Dmel\bgm

Species

D. melanogaster

Name

bubblegum

Annotation symbol

CG4501

Feature type

protein_coding_gene

FlyBase ID

FBgn0027348

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

34F1-34F1

Sequence location

2L:14,013,748..14,019,328 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene bubblegum is referred to in FlyBase by the symbol bgm (CG4501, FBgn0027348). It has the cytological map location 34F1. Its sequence location is 2L:14013748..14019328. Its molecular function is described as long-chain-fatty-acid-CoA ligase activity. It is involved in the biological process long-chain fatty acid metabolic process. 9 alleles are reported. The phenotypes of these alleles are annotated with: optic lobe; lamina. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

34F1-34F1

Limits computationally determined from genome sequence between P{lacW}Sos^k05224&P{lacW}RpII33^k05605 and P{lacW}l(2)34Fa^k00811&P{PZ}wb⁰⁹⁴³⁷

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

34F1-34F2

(determined by in situ hybridisation)

(Ashburner et al., 1999)

34F1-34F2

(determined by in situ hybridisation)

(Spradling et al., 1999)

34F1-34F2

(Min and Benzer, 1999)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

In direction of increasing cytology: CG33681+ tRNA:Q:34E- CG33681+ bgm+ l(2)34Fa-

(Ashburner et al., 1999)

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\bgm for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

bgm-RA

FBtr0080590

NM_079959

2499

666

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

bgm-PA

FBpp0080167

73.6

666

8.38

NP_524698

AAF53368

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0027348

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

AMP-dependent synthetase and ligase (IPR000873)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9V3S9

Maps to

(Ashburner et al., 1999.10.12)

Does NOT map to

Identified with

(Oliver, 2001.8.16)

(Ashburner et al., 1999.10.12)

GM14009

(Ashburner et al., 1999.10.12, BDGP Project Members, 2000-)

LD10778

(Min, 1999.7.28, Ashburner et al., 1999, Ashburner et al., 1999.10.12)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

7623

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0027348

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0027348

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0027348

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for bgm

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for bgm

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

bgm^EY03176

Other Phenotypes

Allele

aging defective | recessive

bgm¹

visual behavior defective | recessive

bgm¹

Sterility

Allele

fertile

bgm^EY03176

Phenotype manifest in

Allele

optic lobe

bgm¹

lamina

bgm¹

retina & cell body

bgm¹

photoreceptor cell & axon

bgm¹

Classical Alleles ( 8 )

For All Classical Alleles Show

Allele of bgm	Mutagen	Stocks	Known lesion
bgm¹	P-element activity	0	Yes
bgm^BG01356	P-element activity	1	--
bgm^EY03176	P-element activity	1	--
bgm^d00529	P-element activity	1	--
bgm^d00592	P-element activity	0	--
bgm^d09065	P-element activity	1	--
bgm^f00439	piggyBac transposase	1	--
bgm^k09909	P-element activity	0	--

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of bgm	Class	Mutagen	Stocks	Known lesion
bgm^GD11331		in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes

Aneuploid Aberrations

Useful deficiency

Df(2L)fn7

Useful duplication

Dp(2;2)GYL

Disrupted in

Df(2L)Exel8032

(Ryder, 2004.4.26)

Not disrupted in

Df(2L)b84h50

(BDGP Project Members, 1994-1999)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD11331}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{WH}bgm^f00439

P{GT1}bgm^BG01356

P{wHy}bgm^DG20403

insertion of enhancer trap

P{lacW}bgm^k09909

P{lacW}l(2)SH1380^SH1380

P{lacZ}bgm¹

insertion of mobile activating element

Related Comments

Please look at the allele reports for the complete phenotype data

The bgm mutant shows elevated levels of very long chain fatty acids (VLCFAs) as seen in the human disease adrenoleukodystrophy (ALD).

(Min and Benzer, 1999)

Gene Ontology: Function, Process & Cellular Component ( 3 )

Molecular Function

CV term

Evidence

References

long-chain-fatty-acid-CoA ligase activity

inferred from sequence or structural similarity with NCBI_NP:599216

(FlyBase, 1992-)

inferred from sequence or structural similarity

(Ashburner et al., 1999)

inferred from mutant phenotype

(Min and Benzer, 1999)

Biological Process

CV term

Evidence

References

long-chain fatty acid metabolic process

inferred from mutant phenotype

(Min and Benzer, 1999)

Cellular Component

CV term

Evidence

References

cellular_component

no biological data available

(FlyBase Curators, 2002-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

bgm allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

68840

Orthologs

Genome-wide drosophilid orthologs

Dana\GF14276

Dere\GG23906

Dgri\GH11132

Dmoj\GI12714

Dper\GL21255

Dpse\GA18225

Dsec\GM15457

Dsim\GD23948

Dvir\GJ18165

Dwil\GK15027

Dyak\GE19019

Curated drosophilid orthologs

Dpse\GA18225

Linkouts

InParanoid orthologs - Eukaryotic orthologs

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ENSANGP00000013234
Anopheles gambiae (Ensembl)
ENSBTAP00000012001
Bos taurus (Ensembl)
ENSCAFP00000027617
Canis familiaris (Ensembl)
NEWSINFRUP00000136648
Takifugu rubripes (Ensembl)
ENSGALP00000002679
Gallus gallus (Ensembl)
ENSP00000252669
Homo sapiens (Ensembl)
ENSMMUP00000024921
Macaca mulatta (Ensembl)
ENSMODP00000001733
Monodelphis domestica (Ensembl)
ENSPTRP00000017616
Pan troglodytes (Ensembl)
ENSRNOP00000016104
Rattus norvegicus (Ensembl)
GSTENP00028995001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000021525
Xenopus tropicalis (Ensembl)
ZDB-GENE-030131-7099
Danio rerio (Zfin)
DDB0190808
Dictyostelium discoideum (Dictybase)
MGI:2385656
Mus musculus (Informatics)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 8 )

v34853

w¹¹¹⁸; P{GD11331}v34853

v34854

w¹¹¹⁸; P{GD11331}v34854

Bloomington

12755

w¹¹¹⁸; P{GT1}bgm^BG01356

21732

y¹ w^67c23; P{wHy}bgm^DG20403

19994

y¹ w^67c23; P{EPgy2}bgm^EY03176

Genomic Clones ( 1 )

BACR09N24

cDNA Clones ( 34 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

GM14009

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0027348

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0027348

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for identity of: BG:DS01514.2 CG4501

(Kodira, 1999.11)

Source for database merge of

Additional comments

Comments About Role

Comments About Molecular Function

Other Comments

Identification: In a P-element screen for mutants that cause reduced life span at 29^oC. Etymology: The appellation bubblegum refers to the bubbly appearance of the lamina in the mutant. bgm mutant flies provide a potential model system for studying mechanisms of neurogenerative disease and screening drugs for treatment of ALD.

(Min and Benzer, 1999)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•