Gene Dmel\Grip163

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General Information

Symbol

Dmel\Grip163

Species

D. melanogaster

Name

Grip163

Annotation symbol

CG5688

Feature type

protein_coding_gene

FlyBase ID

FBgn0026432

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

68F1-68F1

Sequence location

3L:11,986,367..11,991,177 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol Grip163 (CG5688, FBgn0026432). It has the cytological map location 68F1. Its sequence location is 3L:11986367..11991177. Its molecular function is described as gamma-tubulin binding. It is involved in the biological processes: mitosis; mitotic spindle organization and biogenesis. 5 alleles are reported. The phenotypes of these alleles are annotated with: S2 cell-line; spindle; adult external abdomen. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

68F1-68F1

Limits computationally determined from genome sequence between P{PZ}CycA⁰²⁴⁶¹&P{PZ}CycA⁰³⁹⁴⁶ and P{PZ}rols⁰⁸²³²&P{lacW}l(3)j2D3^j2D3

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

68F-68F

(determined by in situ hybridisation)

(Gunawardane et al., 2000)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Grip163 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Grip163-RA

FBtr0076061

NM_140280

4569

1351

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Grip163-PA

FBpp0075793

154.8

1351

6.31

NP_648537

AAF49973

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0026432

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Spc97/Spc98 (IPR007259)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

RE01412

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

4381

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0026432

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0026432

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0026432

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

semi-viable

Grip163^GE27087

lethal

Grip163^CB05139

Other Phenotypes

Allele

mitotic cell cycle defective

Grip163^dsRNA.cVa

Sterility

Allele

female sterile

Grip163^GE27087

Phenotype manifest in

Allele

S2 cell-line

Grip163^dsRNA.cVa

spindle

Grip163^dsRNA.cVa

adult external abdomen

Grip163^GE27087

Classical Alleles ( 2 )

For All Classical Alleles Show

Allele of Grip163	Class	Mutagen	Stocks	Known lesion
Grip163^CB05139		P-element activity	0	Yes
Grip163^GE27087			0	Yes

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Grip163	Mutagen	Stocks	Known lesion
Grip163^GD11734	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
Grip163^T:Zzzz\FLAG	in vitro construct \| coding region fusion	0	Yes
Grip163^dsRNA.cVa	in vitro construct \| RNAi	0	--

Aneuploid Aberrations

Useful deficiency

Df(3L)vin6

Useful duplication

Dp(3;3)M67C⁺5

Disrupted in

Df(3L)Exel6115

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD11734}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{PTT-GB}Grip163^CB05139

insertion of mobile activating element

P{EP}Grip163^GE27087

Related Comments

Please look at the allele reports for the complete phenotype data

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in the failure of γ-tubulin to localize to the spindle, without affecting its localization to the pole, and the formation of an aberrantly long spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

Gene Ontology: Function, Process & Cellular Component ( 6 )

Molecular Function

CV term

Evidence

References

gamma-tubulin binding

inferred from direct assay

(Gunawardane et al., 2003)

Biological Process

CV term

Evidence

References

mitosis

inferred from mutant phenotype

(Verollet et al., 2006)

mitotic spindle organization and biogenesis

inferred from mutant phenotype

(Goshima et al., 2007)

Cellular Component

CV term

Evidence

References

centrosome

inferred from direct assay

(Gunawardane et al., 2000)

gamma-tubulin ring complex

inferred from physical interaction with FLYBASE:γTub23C; FB:FBgn0004176 AND inferred from sequence or structural similarity with EMBL:AJ291606; protein_id:CAC21238.1 AND inferred from sequence or structural similarity with FLYBASE:Grip75; FB:FBgn0026431 AND inferred from sequence or structural similarity with FLYBASE:Grip84; FB:FBgn0026430 AND inferred from sequence or structural similarity with FLYBASE:l(1)dd4; FB:FBgn0001612

(Gunawardane et al., 2000)

non-traceable author statement

(Zheng, 2000.10.30)

spindle pole

inferred from electronic annotation with InterPro:IPR007259

(FlyBase Curators et al., 2004-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Grip163 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

64725

Orthologs

Genome-wide drosophilid orthologs

Dana\GF24490

Dere\GG13861

Dgri\GH15122

Dmoj\GI13774

Dper\GL25062

Dpse\GA19058

Dsec\GM24685

Dsim\GD12755

Dvir\GJ13579

Dwil\GK16936

Dyak\GE20154

Curated drosophilid orthologs

Dmir\CG5688

(Thornton, 2005.12.20)

Dpse\GA19058

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000012762
Anopheles gambiae (Ensembl)
NEWSINFRUP00000134060
Takifugu rubripes (Ensembl)
ENSMODP00000006846
Monodelphis domestica (Ensembl)
ENSRNOP00000043813
Rattus norvegicus (Ensembl)
GSTENP00029665001
Tetraodon nigroviridis (Ensembl)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 1 )

VDRC

v27482

w¹¹¹⁸; P{GD11734}v27482

Genomic Clones ( 2 )

cDNA Clones ( 7 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

RE01412

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0026432

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0026432

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: Grip163 CG5688 Source for merge of Grip163 CG5688 was sequence comparison (date:010111).

(FlyBase, 1996-)

Additional comments

Comments About Role

Comments About Molecular Function

The interaction between Grip163 and γ-Tubulin facilitates γ-Tubulin binding to GTP.

(Gunawardane et al., 2003)

Other Comments

Identification: Protein identified as part of a γ tubulin ring complex.

(Oegema et al., 1999)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_140280

RefSeq (Proteins)

•

NP_648537

DDBJ / EMBL / GenBank

•

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Spc97/Spc98 (IPR007259)

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

64725

DEDB - Drosophila exon database: splicing graphs

•

4381

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0026432

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0026432

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0026432

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0026432

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0026432

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0026432

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000012762
Anopheles gambiae (Ensembl)
NEWSINFRUP00000134060
Takifugu rubripes (Ensembl)
ENSMODP00000006846
Monodelphis domestica (Ensembl)
ENSRNOP00000043813
Rattus norvegicus (Ensembl)
GSTENP00029665001
Tetraodon nigroviridis (Ensembl)

PANTHER - Protein classification by function, families, and pathways

•

FBgn0026432

Synonyms & Secondary IDs ( 5 )

Reported As

Symbol Synonym

CG5688

(Goshima et al., 2007)

Dgrip163

(Stumpff et al., 2005, Cha et al., 2002, Gunawardane et al., 2000, Dias et al., 1999, Oegema et al., 1999, Verollet et al., 2006, Goshima et al., 2007, Vogt et al., 2006, Goshima et al., 2007)

Dgrips163

(Gunawardane et al., 2003)

grip163

(Zheng, 2000.10.30)

Grip163

(Buszczak et al., 2007)

Name Synonym

Grip163

Secondary FlyBase IDs

FBgn0036238

References ( 20 )

Research paper

Buszczak et al., 2007, Genetics 175(3): 1505--1531: The Carnegie protein trap library: A versatile tool for Drosophila developmental studies. [FBrf0200345]
Goshima et al., 2007, Science 316(5823): 417--421: Genes required for mitotic spindle assembly in Drosophila S2 cells. [FBrf0201458]
Verollet et al., 2006, J. Cell Biol. 172(4): 517--528: Drosophila melanogaster [gamma]-TuRC is dispensable for targeting [gamma]-tubulin to the centrosome and microtubule nucleation. [FBrf0190946]
Vogt et al., 2006, Development 133(20): 3963--3972: The γTuRC components Grip75 and Grip128 have an essential microtubule-anchoring function in the Drosophila germline. [FBrf0192931]
Stumpff et al., 2005, Curr. Biol. 15(17): 1525--1534: Drosophila Wee1 Interacts with Members of the gammaTURC and Is Required for Proper Mitotic-Spindle Morphogenesis and Positioning. [FBrf0187336]
Gunawardane et al., 2003, Molec. Biol. Cell 14(3): 1017--1026: Characterization of a new gammaTuRC subunit with WD repeats. [FBrf0159185]
Cha et al., 2002, Nature Cell Biol. 4(8): 592--598: Kinesin I-dependent cortical exclusion restricts pole plasm to the oocyte posterior. [FBrf0151439]
Gunawardane et al., 2000, J. Cell Biol. 151(7): 1513--1524: Characterization and reconstitution of Drosophila gamma-tubulin ring complex subunits. [FBrf0132373]
Oegema et al., 1999, J. Cell Biol. 144(4): 721--733: Characterization of two related Drosophila gamma-tubulin complexes that differ in their ability to nucleate microtubules. [FBrf0107918]

Supplementary material

Goshima et al., 2007, Science 316(5823):: Supporting online material for Genes required for mitotic spindle assembly in Drosophila S2 cells. [FBrf0200397]

Personal communication to FlyBase

Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms.: Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]
Ryder, 2004.4.26, Exelixis CG deletion data.: Exelixis CG deletion data. [FBrf0184335]

Abstract

Dias et al., 1999, Molec. Biol. Cell 10(Suppl.): 19a: Three-dimensional structure of -tubulin ring complex. [FBrf0127501]

FlyBase analysis

FlyBase, 1996-, FlyBase inference based on genome sequence analysis.: FlyBase inference based on genome sequence analysis. [FBrf0104946]
FlyBase, 1992-, FlyBase curation: FlyBase curation. [FBrf0105495]

DNA/RNA sequence record

Thornton, 2005.12.20, GenBank/EMBL/DDBJ: DQ334187: Drosophila miranda CG5688 gene, partial cds. [FBrf0197795]
Zheng, 2000.10.30, GenBank/EMBL/DDBJ: AJ291605: Drosophila melanogaster mRNA for gamma tubulin ring protein (grip163 gene). [FBrf0133180]

Computer file

BDGP Project Members, 2000-, Berkeley Drosophila Genome Project.: Berkeley Drosophila Genome Project. [FBrf0125078]

Automatic genome annotation

FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126705]