A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Gene Dmel\RpII33

General Information
SymbolDmel\RpII33SpeciesD. melanogaster
NameRNA polymerase II 33kD subunitAnnotation symbolCG7885
Feature typeprotein_coding_geneFlyBase IDFBgn0026373
Created / Updated2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)2LRecombination map
Cytogenetic map34D1-34D6Sequence location2L:13,833,339..13,834,306 [+]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene RNA polymerase II 33kD subunit is referred to in FlyBase by the symbol RpII33 (CG7885, FBgn0026373). It has the cytological map location 34D1-34D6. Its sequence location is 2L:13833339..13834306. Its molecular function is described as: DNA-directed RNA polymerase activity; DNA binding; protein dimerization activity. It is involved in the biological process transcription from RNA polymerase II promoter. 7 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides.

hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
34D1-34D6  
Limits computationally determined from genome sequence between P{lacW}Sosk05224&P{lacW}RpII33k05605 and P{lacW}l(2)34Fak00811&P{PZ}wb09437  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
34D-34E
 
(Garesse, 1999.3.8)
34D6-34D7
(determined by in situ hybridisation)  
(Ashburner et al., 1999)
34D6-34D7
(determined by in situ hybridisation)  
(Spradling et al., 1999)
34D6-34D7
 
(BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
In direction of increasing cytology: DNApol-γ35- RpII33+ CG8997-
(Ashburner et al., 1999)
Gene Order (overall orientation not stated)
References
Overall orientation not stated: RpII33- DNApol-γ35+ Orc5- Sop2+ Orc5- Sop2+ tam+
(Lefai et al., 2000)
hide Gene Model & Products
Please see the GBrowse view of Dmel\RpII33 for information on other features
detailed view FBtr0080543 FBtr0091627 FBtr0091626 FBtr0080544 FBtr0080545 FBtr0080566 FBtr0080546 FBpp0080120 FBpp0099758 FBpp0088773 FBpp0080121 FBpp0080122 FBpp0080143 FBpp0080123 FBti0039326 FBti0006260 FBti0026321 FBti0065184 FBti0065708 FBti0101143 FBti0021285 FBti0044978
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
RpII33-RA
FBtr0080544
 NM_058071
  968
  275
RpII33-RB
FBtr0080545
 NM_165064
  912
  117
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
RpII33-PA  
FBpp0080121  
31.2  
275  
4.45  
  NP_477419
  AAF53342
RpII33-PB  
FBpp0080122  
13.1  
117  
4.42  
  NP_723846
  AAN10853
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
•
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
DNA-directed RNA polymerase, 30-40 kDa subunit, conserved site (IPR001514)
DNA-directed RNA polymerase, RBP11-like (IPR009025)
DNA-directed RNA polymerase, dimerisation (IPR011261)
DNA-directed RNA polymerase, insert (IPR011262)
DNA-directed RNA polymerase, RpoA/D/Rpb3-type (IPR011263)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AA264445
 
AA391035
 
AA816736
 
AC092223
 
AE003408
AAF44826
 
AE003641
AAF53342
 
AE003641
AAN10853
 
AI107691
 
AI107720
 
AI108971
 
AI402083
 
AI520264
 
AJ133650
CAB38635
 
AQ025764
 
AY060644
AAL28192
 
BI141964
 
BI172920
 
BI240307
 
BI242962
 
BI585601
 
BI612140
 
BI621528
 
BI629333
 
BI631983
 
BI640820
 
BI640937
 
BP545391
 
BP545392
 
BP545393
 
BP545394
 
BT001794
AAN71549
 
EL886849
 
EL886978
 
L39629
 
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
    Maps to
    (Ashburner et al., 1999.10.12)
    (FlyBase, 1992-)
    Does NOT map to
    Identified with
    (BDGP Project Members, 2000-)
    (Ashburner et al., 1999.10.12)
    (Spradling et al., 1999, Ashburner et al., 1999.10.12)
    (Ashburner et al., 1999, Ashburner et al., 1999.10.12)
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    hide External Data
    Linkouts
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
      Marker for
        Subcellular Localization
        CV Term
        hide Microarray Data
        Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)
        Untitled Document detailed view FBtr0080543 FBtr0091627 FBtr0091626 FBtr0080544 FBtr0080545 FBtr0080566 FBtr0080546
        AI108971
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Lethality
        Allele
        lethal | recessive
        Phenotype manifest in
        Allele
        hide Classical Alleles ( 3 )
        For All Classical Alleles Show

        Allele of RpII33ClassMutagenStocksKnown lesion
        RpII3311 --
        RpII3320 --
        RpII33k056052 Yes
        hide Alleles Carried on Transgenic Constructs ( 4 )
        For All Alleles Carried on Transgenic Constructs Show

        Allele of RpII33ClassMutagenStocksKnown lesion
        RpII33GD47351 Yes
        RpII33MtnA.C.T:Avic\GFP0 Yes
        RpII33MtnA.N.T:Avic\GFP0 Yes
        RpII33Scer\UAS.T:Avic\GFP-EGFP0 Yes
        hide Aneuploid Aberrations
        Useful deficiency
         
        Useful duplication
         
        Not disrupted in
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Fossett et al., 1994)
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Ashburner et al., 1982, Gubb et al., 1990, Fossett et al., 1994, Roote, 1996.8, Roote, 1997.6.3)
        (Ashburner et al., 1982, Gubb et al., 1990, Fossett et al., 1994)
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Fossett et al., 1994)
        (Fossett et al., 1994)
        (Fossett et al., 1994)
        (Fossett et al., 1994)
        (Fossett et al., 1994)
        (Fossett et al., 1994)
        (Fossett et al., 1994)
        (Fossett et al., 1994)
        (Fossett et al., 1994)
        (Fossett et al., 1994)
        (Fossett et al., 1994)
        (Fossett et al., 1994)
        (Fossett et al., 1994)
        (Fossett et al., 1994)
        (Fossett et al., 1994)
        (Fossett et al., 1994)
        (Fossett et al., 1994)
        (Fossett et al., 1994)
        (Roote, 1997.6.3)
        (Gubb et al., 1990)
        (Woodruff and Ashburner, 1979)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Roote, 1997.6.3)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Gubb et al., 1984)
        (Gubb et al., 1984)
        (Gubb et al., 1984)
        (Gubb et al., 1984)
        (Gubb et al., 1984)
        (Gubb et al., 1984)
        (Gubb et al., 1984)
        (Gubb et al., 1984)
        (Gubb et al., 1984)
        (Gubb et al., 1984)
        (Gubb et al., 1984)
        (Woodruff and Ashburner, 1979)
        (Woodruff and Ashburner, 1979)
        (Roote, 1997.6.3)
        (Roote, 1997.6.3)
        (Woodruff and Ashburner, 1979, Ashburner et al., 1982)
        (Woodruff and Ashburner, 1979, Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Woodruff and Ashburner, 1979, Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Woodruff and Ashburner, 1979, Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Woodruff and Ashburner, 1979, Ashburner et al., 1982)
        (Roote, 2000.6.13)
        (Woodruff and Ashburner, 1979)
        (Woodruff and Ashburner, 1979)
        (Gubb et al., 1985)
        (Woodruff and Ashburner, 1979)
        (Roote, 1998.5.15, Ashburner et al., 1999)
        (Woodruff and Ashburner, 1979)
        (Woodruff and Ashburner, 1979)
        (Woodruff and Ashburner, 1979)
        (Ashburner et al., 1982, Fossett et al., 1994)
        Disrupted in
        (Iyengar et al., 1999, Woodruff and Ashburner, 1979)
        (Fossett et al., 1994)
        (Fossett et al., 1994)
        (Ryder, 2004.4.26)
        (Gubb et al., 1990)
        (Gubb et al., 1985)
        (Gubb et al., 1984)
        (Gubb et al., 1984)
        (Alexandrova et al., 1996)
        (Woodruff and Ashburner, 1979, Gubb et al., 1990)
        (Iyengar et al., 1999, Ashburner et al., 1982)
        (Roote, 2000.7.3, Roote, 1995.3.27, Alexandrova et al., 1996, Roote, 1997.6.3)
        (Roote, 1995.3.27)
        (Roote, 2000.7.3, Roote, 1995.3.27, Roote, 1997.6.3)
        (Iyengar et al., 1999, Roote, 2000.7.3, Roote, 1995.3.27, Roote, 1997.6.3)
        (Spradling et al., 1999, BDGP Project Members, 1994-1999)
        (Roote, 2000.7.3, Roote, 1995.3.27, Roote, 1997.6.3)
        (Roote, 2000.7.3, Roote, 1995.3.27, Roote, 1997.6.3)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Roote, 2000.6.13)
        (Roote, 2000.6.13)
        (Roote, 2000.6.13)
        (Roote, 2000.6.13)
        (Roote, 2000.6.13)
        (Roote, 2000.6.5, Roote, 1995.3.27, Roote, 1997.6.3)
        (Roote, 1997.6.3)
        Not duplicated in
        (Roote, 1997.6.3)
        hide Transgenic Constructs & Insertions
        Transgenic Constructs
        Type of construct
        Name
        Expression data
        UAS construct
        P{GD4735}
        NA
        P{UAS-RpII33-EGFP}
        NA
        Insertions
        Type of insertions
        Name
        Expression data
        insertion of enhancer trap
        P{lacW}RpII33k05605
        No
        P{lacW}l(2)SH0272SH0272
        No
        hide Related Comments
        Please look at the allele reports for the complete phenotype data
        RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly long spindles with misaligned chromosomes when assayed in S2 cells in the presence of Cdc27 dsRNA. This phenotype cannot be observed when the screen is performed without Cdc27 dsRNA.
        (Goshima et al., 2007)
        hide Gene Ontology: Function, Process & Cellular Component ( 5 )
        hide Molecular Function
        CV term
        Evidence
        References
        DNA binding
        inferred from electronic annotation with InterPro:IPR001514, InterPro:IPR009025
        (FlyBase Curators et al., 2004-)
        DNA-directed RNA polymerase activity
        non-traceable author statement
        (Garesse, 1999.3.8)
        inferred from sequence or structural similarity with HGNC_gene:POLR2C; HGNC:9189; OMIM:180663 AND inferred from sequence or structural similarity with SGD_LOCUS:RPB3; SGD:S0001283
        (Aoyagi and Wassarman, 2000)
        inferred from sequence or structural similarity
        (Ashburner et al., 1999)
        protein dimerization activity
        inferred from electronic annotation with InterPro:IPR011261, InterPro:IPR011262
        (FlyBase Curators et al., 2004-)
        hide Biological Process
        CV term
        Evidence
        References
        transcription from RNA polymerase II promoter
        inferred from sequence or structural similarity with HGNC_gene:POLR2C; HGNC:9189; OMIM:180663 AND inferred from sequence or structural similarity with SGD_LOCUS:RPB3; SGD:S0001283
        (Aoyagi and Wassarman, 2000)
        hide Cellular Component
        CV term
        Evidence
        References
        DNA-directed RNA polymerase II, core complex
        inferred from sequence or structural similarity with HGNC_gene:POLR2C; HGNC:9189; OMIM:180663 AND inferred from sequence or structural similarity with SGD_LOCUS:RPB3; SGD:S0001283
        (Aoyagi and Wassarman, 2000)
        inferred from sequence or structural similarity
        (Ashburner et al., 1999)
        hide Sequence Ontology: Class of Gene
        protein_coding_gene
         
        nuclear_gene
         
        hide Interactions & Pathways
        hide Summary of Genetic Interactions
        Interacts with
        Please look at the allele data for full details of the genetic interactions
        RpII33 allele
        Gene
        References
        hide External Data
        Linkouts
        BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
        •
        hide Orthologs
        Genome-wide drosophilid orthologs
        Curated drosophilid orthologs
         
        Linkouts
        InParanoid orthologs - Eukaryotic orthologs
        •