Gene Dmel\Sep5

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General Information

Symbol

Dmel\Sep5

Species

D. melanogaster

Name

Sep5

Annotation symbol

CG2916

Feature type

protein_coding_gene

FlyBase ID

FBgn0026361

Created / Updated

2003-12-02/2003-12-02

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

43F8-43F8

Sequence location

2R:3,847,629..3,849,427 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol Sep5 (CG2916, FBgn0026361). It has the cytological map location 43F8. Its sequence location is 2R:3847629..3849427. Its molecular function is described as: GTPase activity; GTP binding; copper ion binding; electron carrier activity; protein binding. It is involved in the biological processes: cytokinesis; cell cycle. 3 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

43F8-43F8

Limits computationally determined from genome sequence between P{lacW}lin19^k01207&P{lacW}rnh1^k07624 and P{lacW}CSN4^k08018&P{lacW}l(2)k08504^k08504

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

43F-43F

Determined by P1 hybridization and P element insertion analysis.

(Hales, 1999.3.12)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Sep5 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Sep5-RA

FBtr0088877

NM_165578

1750

422

Sep5-RB

FBtr0088876

NM_143704

1739

422

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Sep5-PA

FBpp0087953

48.5

422

7.62

NP_724632

AAG22304

Sep5-PB

FBpp0087952

48.5

422

7.62

NP_651961

AAF59161

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0026361

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Cell division/GTP binding protein (IPR000038)

Blue (type 1) copper domain (IPR000923)

Septin (IPR016491)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q7KLG8

Maps to

Does NOT map to

Identified with

LD28935

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AA950395
AI515287

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0026361

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0026361

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0026361

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of Sep5	Class	Mutagen	Stocks	Known lesion
Sep5^f04717		piggyBac transposase	1	--

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Sep5	Class	Mutagen	Stocks	Known lesion
Sep5^GD16661		in vitro construct \| RNAi in vitro construct \| regulatory fusion	0	Yes
Sep5^GD9852		in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes

Aneuploid Aberrations

Useful deficiency

Df(2R)NCX8

Useful duplication

Dp(2;3)GYL2

Disrupted in

Df(2R)Exel6055

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD16661}

P{GD9852}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{WH}Sep5^f04717

P{SUPor-P}KG03733

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 9 )

Molecular Function

CV term

Evidence

References

GTP binding

inferred from electronic annotation with InterPro:IPR000038

(FlyBase Curators et al., 2004-)

GTPase activity

non-traceable author statement

(Hales et al., 1999)

copper ion binding

inferred from electronic annotation with InterPro:IPR000923

(FlyBase Curators et al., 2004-)

electron carrier activity

inferred from electronic annotation with InterPro:IPR000923

(FlyBase Curators et al., 2004-)

protein binding

inferred from electronic annotation with InterPro:IPR016491

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

cell cycle

inferred from electronic annotation with InterPro:IPR000038

(FlyBase Curators et al., 2004-)

cytokinesis

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)

non-traceable author statement

(Pringle et al., 1999)

Cellular Component

CV term

Evidence

References

septin complex

inferred from electronic annotation with InterPro:IPR016491

(FlyBase Curators et al., 2004-)

septin ring

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Sep5 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

68761

Orthologs

Genome-wide drosophilid orthologs

Dana\GF11226

Dere\GG10692

Dper\GL10608

Dpse\GA15516

Dsec\GM20740

Dsim\GD10205

Dwil\GK15728

Dyak\GE23454

Curated drosophilid orthologs

Dpse\GA15516

Linkouts

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 4 )

Harvard

PBac{WH}Sep5^f04717

VDRC

v25454

w¹¹¹⁸; P{GD9852}v25454

v25456

w¹¹¹⁸; P{GD9852}v25456/CyO

Bloomington

13576

y¹ w^67c23; P{SUPor-P}KG03733

Genomic Clones ( 1 )

BACR26A16

cDNA Clones ( 24 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

LD28935

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0026361

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0026361

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: Sep5 CG2916

(Whitfield, 2000.11.1)

Source for merge of: Sep5 CG2916 Source for merge of Sep5 CG2916 was Swiss-Prot update (date:000801).

(FlyBase, 1996-)

Additional comments

The Sep5 gene may be derived from the Sep2 gene by retroposition.

(Betran et al., 2002)

Comments About Role

Comments About Molecular Function

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

dsRNA has been made from templates generated with primers directed against this gene.

(Pilot et al., 2006)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•

UniProtKB/TrEMBL

•

Q7KLG8

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Cell division/GTP binding protein (IPR000038)

Blue (type 1) copper domain (IPR000923)

Septin (IPR016491)

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

68761

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0026361

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0026361

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0026361

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0026361

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0026361

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0026361

PANTHER - Protein classification by function, families, and pathways

•

FBgn0026361

University of Chicago Dev. Expression

•

FBgn0026361

Synonyms & Secondary IDs ( 6 )

Reported As

Symbol Synonym

CG2916

(Ren et al., 2005, Dimova et al., 2003, Asha et al., 2003, Whitfield, 2000.11.1, Goldstein and Gunawardena, 2000, Pilot et al., 2006)

Sep

(Asha et al., 2003)

sep5

(Hales et al., 2000)

Sep5

(Langille and Clark, 2007, Hueber et al., 2007, Bakal, 2007)

Name Synonym

Sep5

septin

(Sisson et al., 2000, Guild et al., 1999)

Septin-5

(Bakal et al., 2007)

Secondary FlyBase IDs

FBgn0033242

References ( 32 )

Generate a list of

All references relating to this gene ( 32 )

List References by type

Recent research papers ( 4 )

Bakal, 2007, Science 316(5832): 1753--1756: [FBrf0204467]
Hueber et al., 2007, Development 134(2): 381--392: Comparative analysis of Hox downstream genes in Drosophila. [FBrf0193798]
Langille and Clark, 2007, Genomics 90(3): 334--343: Parent genes of retrotransposition-generated gene duplicates in Drosophila melanogaster have distinct expression profiles. [FBrf0200094]
Pilot et al., 2006, Development 133(4): 711--723: Developmental control of nuclear morphogenesis and anchoring by charleston, identified in a functional genomic screen of Drosophila cellularisation. [FBrf0190315]

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Recent reviews (0)

All reviews listed in FlyBase were published before 2006

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