Gene Dmel\Tak1

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Tak1-PA

FBpp0077011

75.7

678

5.06

NP_524080

AAF50895

Additional Polypeptide Data & Comments

Reported size (kD)

678 (aa)

Comments

External Data

PANTHER - Protein classification by function, families, and pathways

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FBgn0026323

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Mitogen activated protein kinase kinase kinase-related (IPR015786)

Protein kinase, core (IPR000719)

Tyrosine protein kinase (IPR001245)

Chromogranin/secretogranin (IPR001990)

Serine/threonine protein kinase, active site (IPR008271)

Protein kinase-like (IPR011009)

Protein kinase ATP binding, conserved site (IPR017441)

Serine/threonine protein kinase-related (IPR017442)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9V3Q6

Maps to

Does NOT map to

Identified with

(BDGP Project Members, 2000-)

LD42274

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

deletion

Tak1[3]
X:20,393,369..20,393,401

comment=Mutation is a 31 bp deletion within this region which leads to deletion of the residues VPEDRPSMQYI ending with a stop codon.
evidence=experimental

point mutation

Tak1[2]
X:20,394,298..20,394,298

comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
evidence=experimental
na_change=G20394298T
pr_change=E53@|Tak1-PA
reported_pr_change=E53@

point mutation

Tak1[1]
X:20,393,741..20,393,741

comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. Authors report in paper that mutation is D158G, however their protein sequence in Figure 5 clearly shows that D159G is the correct mutation.
evidence=experimental
na_change=A20393741G
pr_change=D159G|Tak1-PA
reported_pr_change=D159G

point mutation

Tak1[G14]
X:20,393,555..20,393,555

comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
evidence=experimental
na_change=C20393555T
pr_change=T221I|Tak1-PA
reported_pr_change=T221I

(Geuking et al., 2005)

point mutation

Tak1[4]
X:20,393,388..20,393,388

comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
evidence=experimental
na_change=G20393388A
pr_change=R257H|Tak1-PA
reported_pr_change=R257H

rescue fragment

Tak1[+t15]
X:20,382,932..20,398,759

comment=Position of restriction fragment on reference sequence inferred by FlyBase curator.
evidence=experimental
linked_to=BamHI-END_rfrag

External Data

DEDB - Drosophila exon database: splicing graphs

•

5265

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI516414
AI541706

External Data & Images

immune response defective

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

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FBgn0026323

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

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FBgn0026323

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

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FBgn0026323

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

lethal | embryonic stage | recessive

Tak1^dsRNA.cNa

lethal, with Scer\GAL4^unspecified

Tak1^Scer\UAS.cMa

semi-lethal, with Scer\GAL4^GMR.PF

Tak1^Scer\UAS.cTa

Other Phenotypes

Allele

wild-type

Tak1^+t15

wild-type, with Scer\GAL4^en-e16E

wild-type, with Scer\GAL4^arm.PS

visible, with Scer\GAL4^pnr-MD237

Tak1^{K46R.Scer\UAS}

cell death increase, with Scer\GAL4^hs.2sev

Tak1^Scer\UAS.cMa

immune response defective, with Scer\GAL4^hs.PB

Tak1^{dsRNA.Scer\UAS}

immune response defective, with Scer\GAL4^yolk

Tak1^{dsRNA.Scer\UAS}

tissue polarity defective, with Scer\GAL4^hs.2sev

Tak1^Scer\UAS.cMa

Phenotype manifest in

Allele

mesothoracic tergum, with Scer\GAL4^pnr-MD237

microchaeta, with Scer\GAL4^pnr-MD237

mesothoracic tergum, with Scer\GAL4^ap-md544

microchaeta, with Scer\GAL4^ap-md544

embryonic head, with Scer\GAL4^pnr-MD237

Tak1^{K46R.Scer\UAS}

mouth hooks, with Scer\GAL4^pnr-MD237

Tak1^{K46R.Scer\UAS}

embryo | anterior, with Scer\GAL4^pnr-MD237

Tak1^{K46R.Scer\UAS}

embryonic/first instar larval cuticle | dorsal, with Scer\GAL4^pnr-MD237

Tak1^{K46R.Scer\UAS}

embryo, with Scer\GAL4^pnr-MD237

Tak1^{K46R.Scer\UAS}

eye, with Scer\GAL4^GMR.PF

interommatidial bristle, with Scer\GAL4^GMR.PF

Tak1^{K46R.Scer\UAS}

secondary pigment cell, with Scer\GAL4^GMR.PF

Tak1^{K46R.Scer\UAS}

tertiary pigment cell, with Scer\GAL4^GMR.PF

Tak1^{K46R.Scer\UAS}

eye photoreceptor cell, with Scer\GAL4^hs.2sev

Tak1^Scer\UAS.cMa

eye, with Scer\GAL4^hs.2sev

Tak1^Scer\UAS.cMa

eye disc, with Scer\GAL4^hs.2sev

Tak1^Scer\UAS.cMa

ommatidium, with Scer\GAL4^hs.2sev

Tak1^Scer\UAS.cMa

pupa, with Scer\GAL4^GMR.PF

Tak1^Scer\UAS.cTa

eye disc, with Scer\GAL4^GMR.PF

Tak1^Scer\UAS.cTa

photoreceptor cell, with Scer\GAL4^GMR.PF

Tak1^Scer\UAS.cTa

denticle belt, with Scer\GAL4^prd.RG1

Tak1^Scer\UAS.cTa

denticle row 1, with Scer\GAL4^prd.RG1

Tak1^Scer\UAS.cTa

embryonic/first instar larval cuticle | anterior

Tak1^dsRNA.cNa

Classical Alleles ( 9 )

For All Classical Alleles Show

Allele of Tak1	Class	Mutagen	Known lesion
Tak1¹⁷⁹	loss of function	ethyl methanesulfonate	Yes
Tak1¹		ethyl methanesulfonate	Yes
Tak1²⁵²⁷	amorph		Yes
Tak1²		ethyl methanesulfonate	Yes
Tak1³		ethyl methanesulfonate	Yes
Tak1⁴		ethyl methanesulfonate	Yes
Tak1^G14		ethyl methanesulfonate	Yes
Tak1^{MtnA.T:Ivir\HA1}			Yes
Tak1^unspecified			--

Alleles Carried on Transgenic Constructs ( 15 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Tak1	Mutagen	Known lesion
Tak1^+t15	in vitro construct \| genomic fragment	Yes
Tak1^{D159A.Scer\UAS}	in vitro construct \| site directed mutagenesis	Yes
Tak1^GD9587	in vitro construct \| RNAi in vitro construct \| regulatory fusion	Yes
Tak1^{K46R.M.Scer\UAS}	in vitro construct \| site directed mutagenesis	Yes
Tak1^{K46R.Scer\UAS}	in vitro construct \| regulatory fusion in vitro construct \| amino acid replacement	Yes
Tak1^{Scer\UAS.T:Zzzz\FLAG}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	Yes
Tak1^Scer\UAS.cMa	in vitro construct \| regulatory fusion	Yes
Tak1^Scer\UAS.cTa	in vitro construct \| regulatory fusion	Yes
Tak1^{dsRNA.Scer\UAS}	in vitro construct \| regulatory fusion in vitro construct \| RNAi	Yes
Tak1^dsRNA.cBa	in vitro construct \| RNAi	Yes
Tak1^dsRNA.cCa	in vitro construct \| RNAi	Yes
Tak1^dsRNA.cNa	in vitro construct \| RNAi	Yes
Tak1^dsRNA.cSa	in vitro construct \| RNAi	Yes
Tak1^dsRNA.cSb	in vitro construct \| RNAi	Yes
Tak1^{Δ.MtnA.T:Zzzz\FLAG}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	Yes

Aneuploid Aberrations

Useful deficiency

Df(1)HM44

Useful duplication

Dp(1;1)B^STAG

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

characterization construct

P{dTAK1⁺}

Insertions

Type of insertions

Name

Expression data

Related Comments

Please look at the allele reports for the complete phenotype data

Ectopic expression of Tak1 in the eye leads to a small eye phenotype due to ectopically induced apoptosis.

(FlyBase Curators et al., 2004-)

Gene Ontology: Function, Process & Cellular Component ( 20 )

Molecular Function

CV term

Evidence

References

ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR001245, InterPro:IPR017441, InterPro:IPR017442

JUN kinase kinase kinase activity

inferred from mutant phenotype

MAP kinase kinase kinase activity

inferred from sequence or structural similarity

(Danos et al., 1999)

inferred from sequence or structural similarity

inferred from sequence or structural similarity with NCBI_NP:663305

(Brennan and Anderson, 2004)

traceable author statement

protein kinase activity

non-traceable author statement

(Morrison et al., 2000)

protein tyrosine kinase activity

inferred from electronic annotation with InterPro:IPR001245

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

I-kappaB kinase/NF-kappaB cascade

inferred from mutant phenotype

JNK cascade

traceable author statement

inferred from genetic interaction with FLYBASE:bsk; FB:FBgn0000229 AND inferred from genetic interaction with FLYBASE:hep; FB:FBgn0010303

activation of JNKK activity

inferred from mutant phenotype

apoptosis

inferred from mutant phenotype

inferred from genetic interaction with FLYBASE:W; FB:FBgn0003997 AND inferred from genetic interaction with FLYBASE:grim; FB:FBgn0015946 AND inferred from genetic interaction with FLYBASE:rpr; FB:FBgn0011706

compound eye development

inferred from mutant phenotype

defense response

non-traceable author statement

(Lemaitre, 2000.12.20)

dorsal closure

inferred from mutant phenotype

traceable author statement

(Harden, 2002)

immune response

inferred from mutant phenotype

(Leclerc and Reichhart, 2004)

traceable author statement

innate immune response

traceable author statement

(Kurz and Ewbank, 2003)

notum morphogenesis

inferred from mutant phenotype

ommatidial rotation

inferred from mutant phenotype

positive regulation of antibacterial peptide biosynthetic process

traceable author statement

(Brennan and Anderson, 2004)

protein amino acid phosphorylation

non-traceable author statement

(Morrison et al., 2000)

signal transduction

non-traceable author statement

(Lemaitre, 2000.12.20)

Cellular Component

CV term

Evidence

References

cellular_component

no biological data available

(FlyBase Curators, 2002-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Tak1 allele

Gene

References

(McEwen et al., 2000)

Tak1^Scer\UAS.cMa

msn

hep

nmo

bsk

Jra

Tak1^Scer\UAS.cTa

hep

bsk

(Polaski et al., 2006, Geuking et al., 2005)

arm

(McEwen et al., 2000)

unspecified

Traf4

(Cha et al., 2003)

egr

slpr

(Polaski et al., 2006)

External Data

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

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64981

Orthologs

Genome-wide drosophilid orthologs

Dana\GF15984

Dere\GG17579

Dgri\GH17735

Dmoj\GI15482

Dper\GL15931

Dpse\GA14958

Dsec\GM22666

Dsim\Tak1

Dvir\GJ19194

Dwil\GK20056

Dyak\Tak1

Curated drosophilid orthologs

Dpse\GA14958

Dsim\Tak1

Dyak\Tak1