Gene Dmel\eIF5B

Help

Close All Open All

General Information

Symbol

Dmel\eIF5B

Species

D. melanogaster

Name

eIF5B

Annotation symbol

CG10840

Feature type

protein_coding_gene

FlyBase ID

FBgn0026259

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

63E1-63E1

Sequence location

3L:3,428,654..3,460,602 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol eIF5B (CG10840, FBgn0026259). It has the cytological map location 63E1. Its sequence location is 3L:3428654..3460602. Its molecular function is described as: translation initiation factor activity; DEAD/H-box RNA helicase binding; GTP binding; GTPase activity. It is involved in the biological process translational initiation. 7 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

63E1-63E1

Limits computationally determined from genome sequence between P{PZ}kst⁰¹³¹⁸ and P{PZ}Sc2⁰⁵⁶³⁴

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

63D-63D

(Carrera, 1999.4.14)

63D2-63D3

(Lasko, 1999.3.3)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\eIF5B for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

eIF5B-RB

FBtr0073144

NM_143717

4117

1144

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

eIF5B-PB

FBpp0073003

127.2

1144

5.17

NP_651974

AAF47774

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0026259

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Protein synthesis factor, GTP-binding (IPR000795)

Translation elongation factor EFTu/EF1A, domain 2 (IPR004161)

Small GTP-binding protein (IPR005225)

Translation elongation and initiation factors/Ribosomal, beta-barrel (IPR009000)

Translation initiation factor 2 related (IPR015760)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

DS01105

(Carrera et al., 2000)

Does NOT map to

Identified with

AT30361

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

2238

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI260108

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0026259

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0026259

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0026259

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

eIF5B^EY01401

lethal | recessive

lethal | larval stage | recessive

eIF5B^unspecified

Sterility

Allele

fertile

eIF5B^EY01401

Phenotype manifest in

Allele

Classical Alleles ( 6 )

For All Classical Alleles Show

Allele of eIF5B	Mutagen	Stocks	Known lesion
eIF5B⁰⁹¹⁴³		1	Yes
eIF5B^EY01401	P-element activity	1	--
eIF5B^KG09489	P-element activity	1	--
eIF5B^unspecified		0	--
eIF5B^Δ1	Δ2-3	0	Yes
eIF5B^Δ2	Δ2-3	0	Yes

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of eIF5B	Class	Mutagen	Stocks	Known lesion
eIF5B^GD7105		in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes

Aneuploid Aberrations

Useful deficiency

Df(3L)1227

Useful duplication

Dp(3;3)SMG38

Disrupted in

Df(3L)G5

(Carrera et al., 2000)

Df(3L)HR119

(Carrera et al., 2000)

Df(3L)HR232

(Carrera et al., 2000)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD7105}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{?lacW}eIF5B^Δ1

P{?lacW}eIF5B^Δ2

P{PTT-GB}CB05358

P{SUPor-P}eIF5B^KG09489

insertion of enhancer trap

P{PZ}eIF5B⁰⁹¹⁴³

Yes

P{lacW}L3659

insertion of mobile activating element

P{EPgy2}EY03976a

P{EPgy2}eIF5B^EY01401

Related Comments

Please look at the allele reports for the complete phenotype data

Mutations in eIF5B are recessive lethal. Ovaries derived from homozygous germ line clones contain fewer egg chambers than normal, and those egg chambers that do form often have too many nurse cells and two oocytes.

(Johnstone et al., 1999)

Gene Ontology: Function, Process & Cellular Component ( 7 )

Molecular Function

CV term

Evidence

References

DEAD/H-box RNA helicase binding

traceable author statement

(Johnstone and Lasko, 2001)

GTP binding

inferred from electronic annotation with InterPro:IPR000795, InterPro:IPR004161, InterPro:IPR005225

(FlyBase Curators et al., 2004-)

GTPase activity

inferred from electronic annotation with InterPro:IPR000795

(FlyBase Curators et al., 2004-)

translation initiation factor activity

inferred from sequence or structural similarity

(Lasko, 2000)

inferred from sequence or structural similarity with SGD_LOCUS:FUN12; SGD:S0000033

(FlyBase, 1992-)

Biological Process

CV term

Evidence

References

translational initiation

inferred from sequence or structural similarity

(Lasko, 2000)

inferred from sequence or structural similarity with SGD_LOCUS:FUN12; SGD:S0000033

(FlyBase, 1992-)

Cellular Component

CV term

Evidence

References

cytoplasm

inferred from sequence or structural similarity

(Lasko, 2000)

cytosol

inferred from sequence or structural similarity with SGD_LOCUS:FUN12; SGD:S0000033

(FlyBase, 1992-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

eIF5B allele

Gene

References

eIF5B^Δ1

vas

(Carrera et al., 2000)

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

68928

Orthologs

Genome-wide drosophilid orthologs

Dana\GF10267

Dere\GG14257

Dgri\GH15356

Dmoj\GI12960

Dper\GL13259

Dpse\GA10589

Dsec\GM14051

Dsim\GD13328

Dvir\GJ13104

Dwil\GK20376

Dyak\GE20685

Curated drosophilid orthologs

Dpse\GA10589

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000010768
Anopheles gambiae (Ensembl)
ENSBTAP00000005974
Bos taurus (Ensembl)
ENSBTAP00000018796
Bos taurus (Ensembl)
ENSCAFP00000003315
Canis familiaris (Ensembl)
ENSCINP00000018976
Ciona intestinalis (Ensembl)
NEWSINFRUP00000170273
Takifugu rubripes (Ensembl)
ENSGALP00000027015
Gallus gallus (Ensembl)
ENSP00000289371
Homo sapiens (Ensembl)
ENSMMUP00000024930
Macaca mulatta (Ensembl)
ENSMODP00000001676
Monodelphis domestica (Ensembl)
ENSRNOP00000014877
Rattus norvegicus (Ensembl)
GSTENP00012539001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000041985
Xenopus tropicalis (Ensembl)
Q6L4R8
Oryza sativa (Gramene)
AT1G76810.1
Arabidopsis thaliana (Arabidopsis)
WBGene00035824
Caenorhabditis briggsae (Wormbase)
WBGene00021857
Caenorhabditis elegans (Wormbase)
DDB0206214
Dictyostelium discoideum (Dictybase)
MGI:2441772
Mus musculus (Informatics)
YAL035W
Saccharomyces cerevisiae (Yeastgenome)
SPAC56F8.03
Schizosaccharomyces pombe (Sanger)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 7 )

VDRC

v31365

w¹¹¹⁸; P{GD7105}v31365

v31366

w¹¹¹⁸; P{GD7105}v31366/CyO

Bloomington

19641

y¹ w^67c23; P{EPgy2}eIF5B^EY01401

15698

y¹ w^67c23; P{EPgy2}EY03976a P{EPgy2}G-iα65A^EY03976b

10163

y¹ w¹¹¹⁸; P{lacW}L3659, l(3)L3659^L3659/TM3, Ser¹

14984

w¹¹¹⁸; P{SUPor-P}eIF5B^KG09489 ry⁵⁰⁶/TM3, Sb¹ Ser¹

11735

P{PZ}eIF5B⁰⁹¹⁴³, l(3)09143⁰⁹¹⁴³ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹

Genomic Clones ( 3 )

cDNA Clones ( 52 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones