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Gene Dmel\sav

General Information
Symbol	Dmel\sav	Species	D. melanogaster
Name	salvador	Annotation symbol	CG33193
Feature type	protein_coding_gene	FlyBase ID	FBgn0053193
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	3R	Recombination map	3-
Cytogenetic map	94D10-94D10	Sequence location	3R:18,884,329..18,886,991 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene salvador is referred to in FlyBase by the symbol sav (CG33193, FBgn0053193). It has the cytological map location 94D10. Its sequence location is 3R:18884329..18886991. Its molecular function is described as protein binding. It is involved in the biological processes described with 13 unique terms, many of which group under: regulation of biological process; anatomical structure development; programmed cell death; cell proliferation; sensory organ development; regulation of developmental process; positive regulation of programmed cell death; cell communication; response to ionizing radiation; cell cycle. 25 alleles are reported. The phenotypes of these alleles are annotated with 14 unique terms, many of which group under: adult; peripheral nervous system; nervous system; organ system; adult segment; thoracic segment; imaginal precursor; anatomical structure; adult mesothoracic segment; morphogenetic furrow; sense organ; cell cycle; dorsal thoracic disc. It has one annotated transcript and one annotated polypeptide.
External Summaries
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 94D10-94D10   Limits computationally determined from genome sequence between P{lacW}GclmL0580 and P{EP}hhEP3521
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location	3- (Tapon and Hariharan, 1999)
Left of (cM)	hh P{lacW}crbj1B5 (Kango-Singh et al., 2002)
Right of (cM)	klg P{SUPor-P}KG05850 P{lacW}howE7-3-4 (Kango-Singh et al., 2002)
Notes	Located on the right arm of chromosome 3 (Tapon and Hariharan, 1999)
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\sav for information on other features
Comments on Gene Model
	Gene merge based on EST data
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) sav-RA FBtr0084356  NM_176544   2202   608
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein sav-PA   FBpp0083749   68.1   608   9.92     NP_788721   AAF56090
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0053193
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • WW/Rsp5/WWP (IPR001202) SARAH (IPR011524)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE003741 AAF56090   AI404092   AI543516   AW944627   AX260190   AY069854 AAL39999   AY131211 AAM97280   BF498672   BI355383   BI367071   CG33193   CO323764
UniProtKB/Swiss-Prot	Q9VCR6
UniProtKB/TrEMBL
Maps to
Does NOT map to
Identified with	SD10307 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References point mutation sav[shrp-1] 3R:18,886,641..18,886,641 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C18886641T pr_change=Q89@|sav-PA reported_pr_change=Q89@|FBrf0151946 (Kango-Singh et al., 2002) point mutation sav[4] 3R:18,886,428..18,886,428 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C18886428T pr_change=Q160@|sav-PA reported_pr_change=Q160@|FBrf0151717 (Tapon et al., 2002) point mutation sav[shrp-2] 3R:18,886,425..18,886,425 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C18886425T pr_change=Q161@|sav-PA reported_pr_change=Q161@|FBrf0151946 (Kango-Singh et al., 2002) point mutation sav[2] 3R:18,886,215..18,886,215 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C18886215T pr_change=Q231@|sav-PA reported_pr_change=Q231@|FBrf0151717 (Tapon et al., 2002) point mutation sav[shrp-3] 3R:18,886,188..18,886,188 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C18886188T pr_change=Q240@|sav-PA reported_pr_change=Q240@|FBrf0151946 (Kango-Singh et al., 2002) point mutation sav[shrp-4] 3R:18,886,143..18,886,143 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C18886143T pr_change=R255@|sav-PA reported_pr_change=R255@|FBrf0151946 (Kango-Singh et al., 2002) point mutation sav[shrp-5] 3R:18,885,674..18,885,674 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C18885674T pr_change=Q282@|sav-PA reported_pr_change=Q282@|FBrf0151946 (Kango-Singh et al., 2002) point mutation sav[1] 3R:18,885,653..18,885,653 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C18885653T pr_change=Q289@|sav-PA reported_pr_change=Q289@|FBrf0151717 (Tapon et al., 2002)
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 10415
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AI403760
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0053193 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0053193 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0053193
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal (with Df(3R)hh) sav1 sav2 sav3 sav4 lethal (with sav4) sav1 sav2 sav3 lethal (with sav3) sav2 sav4 lethal (with sav2) sav1 sav3 sav4 lethal (with sav1) sav2 sav4 lethal | recessive sav1 sav2 sav3 sav4 lethal | larval stage (with Df(3R)EB6) sav1 lethal | larval stage (with sav3) sav1 lethal | larval stage (with sav1) sav3 lethal | larval stage | recessive savshrp-1 savshrp-2 savshrp-3 savshrp-4 savshrp-5 savshrp-6 Other Phenotypes Allele visible | somatic clone savshrp-3 small body, with Scer\GAL4da.G32 savScer\UAS.T:Ivir\HA1 large body | somatic clone savshrp-1 savshrp-2 savshrp-3 savshrp-4 savshrp-5 savshrp-6 increased cell number | somatic clone sav1 sav2 sav3 sav4 environmental stress response defective | somatic clone sav3 Phenotype manifest in Allele eye | somatic clone sav1 sav2 sav3 sav4 pigment cell | ectopic | somatic clone sav1 sav3 cell cycle | somatic clone sav3 haltere | somatic clone sav3 savshrp-3 mesothoracic tergum | somatic clone sav3 eye photoreceptor cell | somatic clone sav3 eye disc | somatic clone sav3 savshrp-4 savshrp-5 wing, with Scer\GAL4Bx-MS1096 savScer\UAS.T:Ivir\HA1 adult head | somatic clone savshrp-1 savshrp-2 savshrp-3 savshrp-4 savshrp-5 savshrp-6 eye disc | cell autonomous | somatic clone savshrp-1 ommatidial cluster | somatic clone savshrp-1 photoreceptor cell R8 of yellow ommatidium savshrp-1 photoreceptor cell R8 of pale ommatidium savshrp-1 adult thorax | somatic clone savshrp-3 dorsal mesothoracic disc | somatic clone savshrp-3 savshrp-4 pigment cell | somatic clone | supernumerary savshrp-4
Classical Alleles ( 12 )
For All Classical Alleles Show
Allele of sav Class Mutagen Stocks Known lesion sav1 0 Yes sav2 0 Yes sav3 0 Yes sav4 0 Yes savSH13 ethyl methanesulfonate 0 -- savSH8 ethyl methanesulfonate 0 -- savshrp-1 loss of function ethyl methanesulfonate 0 Yes savshrp-2 loss of function ethyl methanesulfonate 0 Yes savshrp-3 loss of function ethyl methanesulfonate 0 Yes savshrp-4 loss of function ethyl methanesulfonate 0 Yes savshrp-5 loss of function ethyl methanesulfonate 0 Yes savshrp-6 loss of function ethyl methanesulfonate 0 Yes
Alleles Carried on Transgenic Constructs ( 13 )
For All Alleles Carried on Transgenic Constructs Show
Allele of sav Class Mutagen Stocks Known lesion savAct5C.T:Ivir\HA1 in vitro construct | coding region fusion in vitro construct | regulatory fusion 0 Yes savAct5C.T:Zzzz\FLAG in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes savC1.Scer\UAS.T:Ivir\HA1 in vitro construct | deletion 0 Yes savC2.Scer\UAS.T:Ivir\HA1 in vitro construct | deletion 0 Yes savGD16019 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes savGD8128 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes savGMR.PT in vitro construct | regulatory fusion 0 Yes savScer\UAS.T:Ivir\HA1 in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes savWW.Scer\UAS.T:Ivir\HA1 in vitro construct | deletion 0 Yes savshrp6.Act5C.T:Zzzz\FLAG in vitro construct | deletion 0 Yes savΔC1.Scer\UAS.T:Ivir\HA1 in vitro construct | deletion 0 Yes savΔC2.Scer\UAS.T:Ivir\HA1 in vitro construct | deletion 0 Yes savΔC3.Scer\UAS.T:Ivir\HA1 in vitro construct | deletion 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(3R)hh
Useful duplication	Dp(3;3)M95A+13
Disrupted in	Df(3R)Exel6193 (Ryder, 2004.4.26) Df(3R)M95A (Kango-Singh et al., 2002) Df(3R)hh (Tapon et al., 2002, Kango-Singh et al., 2002)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD16019} NA P{GD8128} NA P{UAS-sav.C1.FLAG} NA P{UAS-sav.C2.FLAG} NA P{UAS-sav.FLAG} NA P{UAS-sav.WW.FLAG} NA P{UAS-sav.ΔC1.FLAG} NA P{UAS-sav.ΔC2.FLAG} NA P{UAS-sav.ΔC3.FLAG} NA characterization construct P{GMR-sav.T} NA
Insertions
	Type of insertions Name Expression data
Related Comments
	Please look at the allele reports for the complete phenotype data Mutations in sav abolish developmentally controlled programmed cell death in homozygous clones in the eye. (Tapon and Hariharan, 1999)
Gene Ontology: Function, Process & Cellular Component ( 14 )
Molecular Function
CV term Evidence References
protein binding traceable author statement (Ryoo and Steller, 2003) inferred from physical interaction with FLYBASE:hpo; FB:FBgn0034453 (Polesello et al., 2006)
Biological Process
CV term Evidence References
R8 cell fate specification inferred from mutant phenotype (Mikeladze-Dvali et al., 2005)
apoptosis non-traceable author statement (Tapon, 2002.7.12)
imaginal disc development inferred from mutant phenotype (Kango-Singh et al., 2002)
induction of apoptosis inferred from mutant phenotype (Tapon et al., 2002)
negative regulation of cell proliferation inferred from mutant phenotype (Kango-Singh et al., 2002)
negative regulation of organ growth traceable author statement (Hafen and Stocker, 2003)
regulation of cell cycle inferred from mutant phenotype (Tapon et al., 2002) non-traceable author statement (Tapon, 2002.7.12)
regulation of cell proliferation traceable author statement (Lee and Orr-Weaver, 2003)
regulation of imaginal disc growth traceable author statement (Lee and Orr-Weaver, 2003)
regulation of multicellular organism growth inferred from mutant phenotype (Polesello et al., 2006)
response to ionizing radiation inferred from mutant phenotype (Colombani et al., 2006)
retinal cell programmed cell death inferred from genetic interaction with FLYBASE:W; FB:FBgn0003997 AND inferred from mutant phenotype (Tapon et al., 2002)
signal transduction inferred from electronic annotation with InterPro:IPR011524 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions sav allele Gene References sav3 W (Tapon et al., 2002) hpo (Wu et al., 2003) sav4 hpo (Wu et al., 2003) savGMR.PT wts (Harvey et al., 2003, Pantalacci, 2003) unspecified p53 (Colombani et al., 2006) Rassf (Polesello et al., 2006)
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 76064 Drosophila PIMRider - The Drosophila Protein Interaction map • 522377
Orthologs
Genome-wide drosophilid orthologs	Dana\GF18269 Dere\GG12465 Dmoj\GI10759 Dper\GL27353 Dpse\GA27046 Dsec\GM23596 Dsim\GD18409 Dvir\GJ14361 Dwil\GK13578 Dyak\GE23988
Curated drosophilid orthologs
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000020991 Anopheles gambiae (Ensembl) ENSBTAP00000002017 Bos taurus (Ensembl) ENSCAFP00000021364 Canis familiaris (Ensembl) ENSCINP00000012865 Ciona intestinalis (Ensembl) ENSP00000324729 Homo sapiens (Ensembl) ENSPTRP00000010732 Pan troglodytes (Ensembl) WBGene00025250 Caenorhabditis briggsae (Wormbase) WBGene00020427 Caenorhabditis elegans (Wormbase) ZDB-CDNA-040912-30 Danio rerio (Zfin) ZDB-GENE-040912-28 Danio rerio (Zfin) MGI:1927144 Mus musculus (Informatics)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
VDRC	v24801 w1118; P{GD8128}v24801/TM3 v24802 w1118; P{GD8128}v24802/CyO v46445 w1118; P{GD16019}v46445/CyO v46446 w1118; P{GD16019}v46446/CyO
Genomic Clones ( 2 )
	BACR17P04 BACR01K08
cDNA Clones ( 6 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	SD10307
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH23684 GM30584 RE52745 AT13124
Other clones	EK191405
RNAi & Array Information
Affy Oligo
Linkouts	DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0053193 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0053193
Antibody Information
Other Information
Discoverer
Etymology
	Etymology: the gene is called "shar-pei" because of the mutant folded cuticle phenotype in the head, which resembles the folded skin of Shar-pei dogs. (Kango-Singh et al., 2002)
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: salvador CG13831 was sequence comparison, ma021128.   Source for merge of: salvador CG13831 (Tapon, 2002.7.12) Source for merge of: CG13831 CG13832 Source for merge of CG13831 CG13832 was a shared cDNA (date:010720). Source for merge of: sav anon-WO0172774.152 Source for merge of sav anon-WO0172774.152 was sequence comparison (date:051113). (FlyBase, 1996-)
Additional comments	Annotations CG13831, CG13832 merged as CG33193 in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003)
Comments About Role
	sav promotes both cell cycle exit and apoptosis. (Tapon et al., 2002) sav is required for the proper termination of cell proliferation during imaginal disc development. (Kango-Singh et al., 2002)
Comments About Molecular Function
Other Comments
	Identification: Isolated as a mutation that causes overgrowth in homozygous clones in the eye. (Tapon and Hariharan, 1999) dsRNA has been made from templates generated with primers directed against this gene. (Polesello et al., 2006)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_176544 RefSeq (Proteins) • NP_788721 DDBJ / EMBL / GenBank • AE003741 AAF56090 AI543516 AW944627 AX260190 AY069854 AAL39999 AY131211 AAM97280 UniProtKB/Swiss-Prot • Q9VCR6
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • WW/Rsp5/WWP (IPR001202) SARAH (IPR011524)
Linkouts
	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 76064 DEDB - Drosophila exon database: splicing graphs • 10415 DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0053193