A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Gene Dmel\Adar

General Information
SymbolDmel\AdarSpeciesD. melanogaster
NameAdenosine deaminase acting on RNAAnnotation symbolCG12598
Feature typeprotein_coding_geneFlyBase IDFBgn0026086
Created / Updated2005-09-09/2005-09-09
Genomic Location
Chromosome (arm)XRecombination map
Cytogenetic map2B9-2B9Sequence locationX:1,667,783..1,678,034 [+]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene Adenosine deaminase acting on RNA is referred to in FlyBase by the symbol Adar (CG12598, FBgn0026086). It has the cytological map location 2B9. Its sequence location is X:1667783..1678034. Its molecular function is described as: double-stranded RNA adenosine deaminase activity; double-stranded RNA binding. It is involved in the biological processes: response to hypoxia; adenosine to inosine editing; adult behavior; adult locomotory behavior; regulation of membrane potential; response to heat; locomotory behavior; mRNA modification; RNA processing. 25 alleles are reported. The phenotypes of these alleles are annotated with: adult central nervous system; medulla; lobula; lamina; wing; photoreceptor cell; adult brain; optic lobe; retina. It has 3 annotated transcripts and 3 annotated polypeptides.

External Summaries

hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
2B9-2B9  
Limits computationally determined from genome sequence between P{EP}brEP1515 and P{EP}EP1444&P{EP}CG14818EP1190  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
2B6-2B7
(determined by in situ hybridisation)  
(Palladino et al., 2000)
2B6-2B7
 
(Reenan, 1999.11.27)
2B6-2B7
 
(Reenan et al., 2000)
Experimentally Determined Recombination Data
Location
1-
(Haddad et al., 1997)
1-0.13
(Palladino et al., 2000)
Left of (cM)
(Haddad et al., 1997)
Right of (cM)
(Haddad et al., 1997)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
Gene Order (overall orientation not stated)
References
hide Gene Model & Products
Please see the GBrowse view of Dmel\Adar for information on other features
detailed view FBtr0070300 FBtr0070299 FBtr0100557 FBpp0070287 FBpp0100009 FBpp0070286 FBti0049164 FBti0043643 FBti0052562 FBti0068245 FBti0071237 FBti0018122
Comments on Gene Model
Atypical cDNA: retained intron (SD06892).
DGC clone appears problematic (SD06892): unspliced intron
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
Adar-RA
FBtr0070299
 NM_130584
  2623
  632
Adar-RB
FBtr0070300
 NM_166903
  2556
  628
Adar-RC
FBtr0100557
 NM_001038732
  3426
  186
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
Adar-PA  
FBpp0070286  
70.0  
632  
9.54  
  NP_569940
  AAF45665
Adar-PB  
FBpp0070287  
69.6  
628  
9.47  
  NP_726761
  AAN09057
Adar-PC  
FBpp0100009  
20.8  
186  
9.74  
  NP_001033821
  ABC67164
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
•
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
Double-stranded RNA binding (IPR001159)
Adenosine deaminase/editase (IPR002466)
Double-stranded RNA-binding-like (IPR014720)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AA941852
 
AA951137
 
AA978800
 
AA990768
 
AE003422
AAF45665
 
AE003422
AAN09057
 
AF208535
AAF63702
 
AF208535
AAF63703
 
AF343579
AAK26850
 
AI513657
 
AI541749
 
AL035207
CAA22774
 
AW944473
 
AY118653
AAM50022
 
BH023768
 
BI173943
 
BI353732
 
CO189963
 
CO271176
 
CO277493
 
CO277818
 
CO285414
 
CO295380
 
CO295694
 
CO295809
 
CO298068
 
CO302122
 
CO304088
 
CO320068
 
CO323438
 
CO332188
 
CO333319
 
CO341308
 
CO341362
 
CO343065
 
DN153928
 
EC062191
 
EC065137
 
EC067800
 
EC071229
 
EC083216
 
EC086978
 
EC089992
 
EC201638
 
EC211464
 
EC218355
 
EC223595
 
EC224561
 
EC232078
 
EC232255
 
EC244193
 
EC252166
 
EC252178
 
EC257313
 
EC257345
 
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
    Maps to
    (Palladino et al., 2000, Palladino et al., 2000)
    Does NOT map to
    Identified with
    (Benos et al., 2001)
    (Benos, 2000.5.31)
    (BDGP Project Members, 2000-)
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    hide External Data
    Linkouts
    DEDB - Drosophila exon database: splicing graphs
    •
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
      Marker for
        Subcellular Localization
        CV Term
        hide Microarray Data
        Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)
        Untitled Document detailed view FBtr0070300 FBtr0070299 FBtr0100557
        AI541749
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Lethality
        Allele
        viable, with Scer\GAL4Mef2.PR
        viable, with Adar5G1, Scer\GAL4Cha.7.4
        viable, with Adar3.4.S.Scer\UAS, Scer\GAL4Cha.7.4
        semi-viable | male, with Adar1F4, Scer\GAL4Act5C.PI
        semi-viable | male, with Adar3.4.G.Scer\UAS, Scer\GAL4Act5C.PI
        semi-viable | male, with AdarEA.3.4.Scer\UAS, Scer\GAL4Act5C.PI
        lethal | conditional qualifier | recessive
        lethal, with Scer\GAL4Mef2.PR
        lethal, with Adar5G1, Scer\GAL4Mef2.PR
        lethal, with Adar1F4, Scer\GAL4Act5C.PI
        lethal, with Adar3.4.S.Scer\UAS, Scer\GAL4Mef2.PR
        lethal, with Adar3.4.S.Scer\UAS, Scer\GAL4Act5C.PI
        semi-lethal | male, with Adar1F4, Scer\GAL4Act5C.PI
        semi-lethal | male, with Adar3.4.Scer\UAS, Scer\GAL4Act5C.PI
        Other Phenotypes
        Allele
        wild-type
        short lived
        behavior defective | recessive
        behavior defective | adult stage | recessive | conditional - heat sensitive
        paralytic | adult stage | recessive | conditional - heat sensitive
        courtship defective | male
        environmental stress response defective | recessive
        heat stress response defective
        locomotor behavior defective
        locomotor behavior defective | adult stage | recessive | conditional - heat sensitive
        uncoordinated
        uncoordinated | conditional - heat sensitive
        flight behavior defective | recessive
        grooming behavior defective | recessive
        neurophysiology defective
        neurophysiology defective | adult stage | dominant
        neuroanatomy defective
        Phenotype manifest in
        Allele
        wing
        photoreceptor cell
        adult brain
        optic lobe
        retina
        lamina
        wing | conditional - heat sensitive
        adult central nervous system
        medulla
        lobula
        hide Classical Alleles ( 17 )
        For All Classical Alleles Show

        Allele of AdarClassMutagenStocksKnown lesion
        Adar1F10 Yes
        Adar1F40 Yes
        Adar1G30 Yes
        Adar2B30 Yes
        Adar2J30 Yes
        Adar4A10 Yes
        Adar4F10 Yes
        Adar5A10 Yes
        Adar5G10 Yes
        Adar5I20 Yes
        Adar6A20 Yes
        Adar6C10 Yes
        Adar6K10 Yes
        AdarBG022351 --
        AdarHD570 Yes
        AdarL0 --
        AdarPamorph0 Yes
        hide Alleles Carried on Transgenic Constructs ( 8 )
        For All Alleles Carried on Transgenic Constructs Show

        Allele of AdarClassMutagenStocksKnown lesion
        Adar3.4.G.Scer\UAS0 Yes
        Adar3.4.S.Scer\UAS0 Yes
        Adar3.4.Scer\UAS0 Yes
        Adar3a.Scer\UAS0 Yes
        AdarEA.3.4.Scer\UAS0 Yes
        AdarGD13552 Yes
        AdarScer\UAS.cMa0 Yes
        AdarcWa0 Yes
        hide Aneuploid Aberrations
        Useful deficiency
         
        Useful duplication
         
        Duplicated in
        (Palladino et al., 2000)
        Disrupted in
        (Ma et al., 2001)
        (Ma et al., 2001)
        Not disrupted in
        (Ma et al., 2001)
        hide Transgenic Constructs & Insertions
        Transgenic Constructs
        Type of construct
        Name
        Expression data
        UAS construct
        P{GD1355}
        NA
        P{UAS-Adar.3.4.G}
        NA
        P{UAS-Adar.3.4}
        NA
        P{UAS-Adar.3a}
        NA
        P{UAS-Adar.EA}
        NA
        P{UAS-Adar.M}
        NA
        Insertions
        Type of insertions
        Name
        Expression data
        miscellaneous insertions
        P{GT1}AdarBG02235
        NA
        P{}AdarHD57
        NA
        hide Related Comments
        Please look at the allele reports for the complete phenotype data
        Adults lacking Adar function are morphologically wild type, but show extreme behavioural deficits, including temperature-sensitive paralysis, locomotor uncoordination and tremors which increase in severity with age. Neurodegeneration accompanies the increase in phenotypic severity.
        (Palladino et al., 2000)
        hide Gene Ontology: Function, Process & Cellular Component ( 12 )
        hide Molecular Function
        CV term
        Evidence
        References
        double-stranded RNA adenosine deaminase activity
        inferred from direct assay
        (Reenan et al., 2000)
        inferred from direct assay
        (Palladino et al., 2000)
        double-stranded RNA binding
        inferred from sequence or structural similarity
        (Lasko, 2000)
        inferred from electronic annotation with InterPro:IPR001159
        (FlyBase Curators et al., 2004-)
        hide Biological Process
        CV term
        Evidence
        References
        RNA processing
        inferred from electronic annotation with InterPro:IPR002466
        (FlyBase Curators et al., 2004-)
        adenosine to inosine editing
        inferred from mutant phenotype
        (Palladino et al., 2000)
        adult behavior
        inferred from mutant phenotype
        (Palladino et al., 2000)
        adult locomotory behavior
        inferred from mutant phenotype
        (Palladino et al., 2000)
        inferred from mutant phenotype
        (Ma et al., 2001)
        locomotory behavior
        inferred from mutant phenotype
        (Keegan et al., 2005)
        mRNA modification
        traceable author statement
        (Driscoll and Gerstbrein, 2003)
        inferred from direct assay
        (Keegan et al., 2005)
        inferred from mutant phenotype
        (Ma et al., 2001)
        regulation of membrane potential
        inferred from mutant phenotype
        (Ma et al., 2001)
        response to heat
        inferred from mutant phenotype
        (Ma et al., 2001)
        response to hypoxia
        traceable author statement
        (Driscoll and Gerstbrein, 2003)
        inferred from mutant phenotype
        (Ma et al., 2001)
        hide Cellular Component
        CV term
        Evidence
        References
        intracellular
        inferred from electronic annotation with InterPro:IPR001159, InterPro:IPR014720
        (FlyBase Curators et al., 2004-)
        hide Sequence Ontology: Class of Gene
        protein_coding_gene
         
        nuclear_gene
         
        hide Interactions & Pathways
        hide Summary of Genetic Interactions
        Interacts with
        Please look at the allele data for full details of the genetic interactions
        Adar allele
        Gene
        References
        hide External Data
        Linkouts
        BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
        •
        Drosophila PIMRider - The Drosophila Protein Interaction map
        •
        hide Orthologs
        Genome-wide drosophilid orthologs