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Gene Dmel\Cortactin

General Information
Symbol	Dmel\Cortactin	Species	D. melanogaster
Name	Cortactin	Annotation symbol	CG3637
Feature type	protein_coding_gene	FlyBase ID	FBgn0025865
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	93B8-93B9	Sequence location	3R:16,887,402..16,890,185 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol Cortactin (CG3637, FBgn0025865). It has the cytological map location 93B8-93B9. Its sequence location is 3R:16887402..16890185. Its molecular function is described as structural constituent of cytoskeleton. It is involved in the biological processes: regulation of cell shape; border follicle cell migration; ovarian ring canal formation; cytoskeleton organization and biogenesis. 16 alleles are reported. The phenotypes of these alleles are annotated with: actin filament; nurse cell ring canal; egg; nurse follicle cell; border follicle cell. It has one annotated transcript and one annotated polypeptide.
External Summaries
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 93B8-93B9   Limits computationally determined from genome sequence between P{PZ}Atpα01164 and P{PZ}mod(mdg4)07038&P{lacW}mod(mdg4)L3101
Experimentally Determined Cytological Location
Cytogenetic map Notes References 93B-93B (determined by in situ hybridisation)   (Katsube et al., 2001) 93B3-93B7   (Katsube, 1999.7.16) 93B3-93B6   (Katsube, 1997.12.26)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\Cortactin for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Cortactin-RA FBtr0084039  NM_079702   2439   559
Additional Transcript Data & Comments
Reported size (kB)	2.7 (northern blot) (Katsube et al., 1998)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Cortactin-PA   FBpp0083441   61.1   559   4.82     NP_524426   AAF55840
Additional Polypeptide Data & Comments
Reported size (kD)	559 (aa); 61 (kD predicted) (Katsube et al., 1998)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0025865
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Neutrophil cytosol factor 2 (IPR000108) Src homology-3 (IPR001452) Hs1/Cortactin (IPR003134)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA950022   AB009998 BAA34397   AB030177 BAB01490   AE003733 AAF55840   AI511738   AI541980   AW943001   AY051774 AAK93198   BG635630   BI166016   BI234673   BI237971   BI239167   BI239719   BI243321   BI356864   BI364264   BI365007   BI365848   BI372874   BI373531   BI487230   BI641420   CK604587   CK604588   CK658950   CK661427   CL528205   CO155457   CO156530   CO310609   CO321664   CO335636   CO340307   CZ470613   CZ470677   CZ470678   EC056768   EC063795   EC064700   EC067497   EC074442   EC088592   EC221186   EC263877   EC264105   UT01301
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	O96046 Q9VDF4
Maps to
Does NOT map to
Identified with	LD29964 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 10170
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference  embryonic stage-adult stage   (Katsube et al., 1998)
Marker for
Subcellular Localization
CV Term	intercellular junction (Katsube et al., 1998)
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AA950022
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0025865 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0025865 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0025865 FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Cortactin Stage(s) 4-6 Stage(s) 9-10 Stage(s) 11-12 Stage(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Cortactin6A2 CortactinA4 CortactinD4 CortactinEY10111 CortactinK8 CortactinM7 Sterility Allele fertile CortactinEY10111 female fertile | reduced CortactinA4 CortactinD4 CortactinK8 CortactinM7 Phenotype manifest in Allele nurse cell ring canal CortactinD4 CortactinM7 egg CortactinD4 CortactinM7 nurse follicle cell CortactinD4 CortactinM7 border follicle cell CortactinM7 border follicle cell | cell autonomous | somatic clone CortactinM7 actin filament | ectopic, with Scer\GAL4Act5C.PP CortactinScer\UAS.cSa
Classical Alleles ( 11 )
For All Classical Alleles Show
Allele of Cortactin Class Mutagen Stocks Known lesion Cortactin6A2 P-element activity 1 Yes CortactinA4 Δ2-3 0 Yes CortactinD4 Δ2-3 0 Yes CortactinEPg35301 0 Yes CortactinEY10111 P-element activity 1 -- CortactinEY20304 P-element activity 1 -- CortactinK8 Δ2-3 0 Yes CortactinM7 Δ2-3 0 Yes Cortactinc06340 piggyBac transposase 0 -- Cortactinc06419 piggyBac transposase 0 -- Cortactinneo54 0 --
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Cortactin Class Mutagen Stocks Known lesion CortactinGD11035 in vitro construct | RNAi in vitro construct | regulatory fusion 0 Yes CortactinScer\UAS.T:Ivir\HA1 1 Yes CortactinScer\UAS.cSa in vitro construct | regulatory fusion 0 Yes Cortactinunspecified in vitro construct | regulatory fusion 0 -- CortactinαTub.PS in vitro construct | regulatory fusion 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(3R)e-R1
Useful duplication	Dp(3;3)M95A+13
Disrupted in	Df(3R)Exel6272 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD11035} NA P{UAS-Cortactin.HA3} NA characterization construct P{UAS-Cortactin.S} NA P{tub-cortactin.S} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{PB}Cortactinc06340 NA PBac{PB}Cortactinc06419 NA P{hsneo}Cortactinneo54 NA insertion of mobile activating element P{EPgy2}CortactinEY10111 NA P{EPgy2}CortactinEY20304 NA P{EPg}CortactinEPg35301 NA
Related Comments
	Please look at the allele reports for the complete phenotype data RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in S2R+ cells: cells become retracted (unspread but flat). Kc167 cells are unaffected. (Kiger et al., 2003)
Gene Ontology: Function, Process & Cellular Component ( 6 )
Molecular Function
CV term Evidence References
structural constituent of cytoskeleton inferred from electronic annotation (King et al., 2002.9.23)
Biological Process
CV term Evidence References
border follicle cell migration inferred from mutant phenotype (Somogyi and Rorth, 2004)
cytoskeleton organization and biogenesis inferred from sequence or structural similarity with UniProtKB:Q60598 (FlyBase, 1992-)
ovarian ring canal formation inferred from mutant phenotype (Somogyi and Rorth, 2004)
regulation of cell shape inferred from mutant phenotype (Kiger et al., 2003)
Cellular Component
CV term Evidence References
cytoskeleton inferred from sequence or structural similarity with UniProtKB:Q60598 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Cortactin allele Gene References unspecified Pvr (Somogyi and Rorth, 2004) Src42A (Somogyi and Rorth, 2004)
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 67462
Orthologs
Genome-wide drosophilid orthologs	Dana\GF18522 Dere\GG14937 Dgri\GH23174 Dmoj\GI10305 Dper\GL12684 Dpse\GA17577 Dsec\GM15108 Dsim\GD20012 Dvir\GJ10159 Dwil\GK13516
Curated drosophilid orthologs	Dpse\GA17577
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000029554 Anopheles gambiae (Ensembl) ENSBTAP00000007976 Bos taurus (Ensembl) ENSCAFP00000015402 Canis familiaris (Ensembl) ENSCINP00000017293 Ciona intestinalis (Ensembl) NEWSINFRUP00000172721 Takifugu rubripes (Ensembl) ENSGALP00000012463 Gallus gallus (Ensembl) ENSP00000320176 Homo sapiens (Ensembl) ENSMMUP00000017450 Macaca mulatta (Ensembl) ENSMODP00000022801 Monodelphis domestica (Ensembl) ENSPTRP00000026335 Pan troglodytes (Ensembl) ENSRNOP00000050255 Rattus norvegicus (Ensembl) GSTENP00008340001 Tetraodon nigroviridis (Ensembl) ENSXETP00000008263 Xenopus tropicalis (Ensembl) ZDB-GENE-040715-3 Danio rerio (Zfin) MGI:104568 Mus musculus (Informatics)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	9367 w*; Cortactin6A2/TM6B, Tb1 16968 y1 w67c23; P{EPgy2}CortactinEY10111 9368 y1 w*; P{UAS-Cortactin.HA3}2; MKRS/TM6B, Tb1 22381 y1 w67c23; P{EPgy2}CortactinEY20304/TM3, Sb1 Ser1
Genomic Clones ( 2 )
	BACR05I01 BACR20N14
cDNA Clones ( 27 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD29964
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD43239 SD13090 RE70752 RE49337 RE60846 SD24740 LP20946 RE43125 RE34318 RE30417 RE51237 LP16293 RE06034 SD07972 RE40921 RE50225 RE36288 RE35582 RE60066
Other clones	EN06061 EK222119 EP10404 EN10861 EN08239 UT01301 EK186129
RNAi & Array Information
Affy Oligo
Linkouts	DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0025865 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0025865
Antibody Information
	polyclonal (Katsube et al., 1998)
Other Information
Discoverer
Etymology
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of	Source for identity of: Cortactin CG3637 (Gong, 1999.11)
Source for database merge of
Additional comments
Comments About Role
	Cortactin modulates cell migration and ring canal morphogenesis during oogenesis. (Somogyi and Rorth, 2004)
Comments About Molecular Function
Other Comments
	dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation. (Rogers et al., 2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079702 RefSeq (Proteins) • NP_524426 DDBJ / EMBL / GenBank • AA950022 AB009998 BAA34397 AB030177 BAB01490 AE003733 AAF55840 AW943001 AY051774 AAK93198 CL528205 CZ470613 CZ470677 CZ470678 UniProtKB/TrEMBL • O96046 Q9VDF4
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Neutrophil cytosol factor 2 (IPR000108) Src homology-3 (IPR001452) Hs1/Cortactin (IPR003134)
Linkouts
	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 67462 DEDB - Drosophila exon database: splicing graphs • 10170 DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0025865 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0025865 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0025865 FlyExpress - Embryonic expression images (BDGP data) • FBgn0025865 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0025865 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0025865 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0025865 InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000029554 Anopheles gambiae (Ensembl) ENSBTAP00000007976 Bos taurus (Ensembl) ENSCAFP00000015402 Canis familiaris (Ensembl)