Gene Dmel\Int6

(BDGP Project Members, 1994-1999)

73D1-73D2

(determined by in situ hybridisation)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Int6 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Int6-RA

FBtr0075345

NM_058037

1575

435

Additional Transcript Data & Comments

Reported size (kB)

1.5 (northern blot)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Int6-PA

FBpp0075104

51.2

435

6.77

NP_477385

AAF49412

Additional Polypeptide Data & Comments

Reported size (kD)

435 (aa); 48 (kD observed)

Comments

External Data

PANTHER - Protein classification by function, families, and pathways

•

FBgn0025582

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Proteasome component region PCI (IPR000717)

Translation initiation factor 3, subunit 6, eukaryotic (IPR016650)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

O77410

UniProtKB/TrEMBL

Q8MR88

Maps to

FBcl0297918

(Miyazaki et al., 1999, Spradling et al., 1999, BDGP Project Members, 2000-)

Does NOT map to

Identified with

GM01233

LD13907

(Spradling et al., 1999)

Dm2312

(BDGP Project Members, 1994-1999, Spradling et al., 1999)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

DEDB - Drosophila exon database: splicing graphs

•

3815

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

embryonic stage-adult stage

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

embryonic stage-adult stage

Marker for

Subcellular Localization

CV Term

microsome

External Data & Images

Show all FlyExpress processed images for Int6

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0025582

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0025582

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0025582

FlyExpress - Embryonic expression images (BDGP data)

•

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | recessive

Phenotype manifest in

Allele

Classical Alleles ( 2 )

For All Classical Alleles Show

Allele of Int6	Class	Mutagen	Stocks	Known lesion
Int6¹⁰⁵⁴⁷		P-element activity	1	--
Int6^j9E8		P-element activity	0	--

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Int6	Class	Mutagen	Stocks	Known lesion
Int6^GD14286		in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes

Aneuploid Aberrations

Useful deficiency

Df(3L)st-b11

Useful duplication

Dp(3;3)st⁺g18

Disrupted in

Df(3L)81k19

(Spradling et al., 1999, BDGP Project Members, 1994-1999, Cunniff et al., 1997)

Df(3L)Exel6130

(Ryder, 2004.4.26)

Df(3L)st-b11

In(3L)std11

Not disrupted in

Df(3L)st-g24

(Spradling et al., 1999, BDGP Project Members, 1994-1999, Cunniff et al., 1997)

Df(3L)st-j7

Df(3L)st100.62

Df(3L)st4

(Stroschein-Stevenson et al., 2006)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD14286}

Insertions

Type of insertions

Name

Expression data

insertion of enhancer trap

P{PZ}Int6¹⁰⁵⁴⁷

Yes

P{lacW}Int6^j9E8

Related Comments

Please look at the allele reports for the complete phenotype data

S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

Gene Ontology: Function, Process & Cellular Component ( 5 )

Molecular Function

CV term

Evidence

References

translation initiation factor activity

inferred from sequence or structural similarity with EMBL:AL035216; protein_id:CAA22813

(Swiss-Prot Project Members, 1999.12.15)

inferred from sequence or structural similarity

(Lasko, 2000)

non-traceable author statement

Biological Process

CV term

Evidence

References

phagocytosis, engulfment

inferred from mutant phenotype

(Stroschein-Stevenson et al., 2006)

translational initiation

inferred from sequence or structural similarity with EMBL:AL035216; protein_id:CAA22813

(Swiss-Prot Project Members, 1999.12.15)

inferred from sequence or structural similarity

(Lasko, 2000)

Cellular Component

CV term

Evidence

References

cytosol

non-traceable author statement

eukaryotic translation initiation factor 3 complex

inferred from sequence or structural similarity with EMBL:AL035216; protein_id:CAA22813

(Swiss-Prot Project Members, 1999.12.15)

inferred from sequence or structural similarity

(Lasko, 2000)

non-traceable author statement

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Int6 allele

Gene

References

External Data

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

65176

Orthologs

Genome-wide drosophilid orthologs

Dana\GF10503

Dere\GG15850

Dgri\GH15221

Dmoj\GI11931

Dper\GL16190

Dpse\GA21959

Dsec\GM24367

Dsim\GD12442

Dvir\GJ12157

Dwil\GK12672

Dyak\Int6

Curated drosophilid orthologs

Dmir\CG9677-like

Dpse\GA21959

Dsim\Int6

(Swanson et al., 2001)

Dyak\Int6