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Gene Dmel\BubR1

General Information
Symbol	Dmel\BubR1	Species	D. melanogaster
Name	Bub1-related kinase	Annotation symbol	CG7838
Feature type	protein_coding_gene	FlyBase ID	FBgn0025458
Created / Updated	2003-12-01/2007-12-14
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	42A5-42A6	Sequence location	2R:1,856,600..1,862,122 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Bub1-related kinase is referred to in FlyBase by the symbol BubR1 (CG7838, FBgn0025458). It has the cytological map location 42A5-42A6. Its sequence location is 2R:1856600..1862122. Its molecular function is described as: protein serine/threonine kinase activity; protein kinase activity; ATP binding. It is involved in the biological processes: mitotic cell cycle spindle assembly checkpoint; protein amino acid phosphorylation; regulation of exit from mitosis; spindle checkpoint; syncytial blastoderm mitotic cell cycle; mitotic spindle organization and biogenesis. 11 alleles are reported. The phenotypes of these alleles are annotated with 11 unique terms, many of which group under: anatomical structure; cell cycle; intracellular organelle; developing anatomical structure; spindle; intracellular non-membrane-bounded organelle; intracellular organelle part; cytoskeletal part; nucleus; mitotic cell cycle; imaginal precursor; organ system; spindle microtubule. It has one annotated transcript and one annotated polypeptide.
External Summaries
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 42A5-42A6   Limits computationally determined from genome sequence between P{PZ}l(2)0985109851&P{lacW}Src42Ak10108 and P{lacW}l(2)k09848k09848&P{EP}EP407
Experimentally Determined Cytological Location
Cytogenetic map Notes References 42A1-42A2 (determined by in situ hybridisation)   (Spradling et al., 1999) 42A1-42A3 (determined by in situ hybridisation)   (Basu et al., 1999) 42A1-42A2   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\BubR1 for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) BubR1-RA FBtr0086032  NM_058034   4862   1460
Additional Transcript Data & Comments
Reported size (kB)	4.601 (longest cDNA) (Basu et al., 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein BubR1-PA   FBpp0085368   165.1   1460   6.27     NP_477382   AAF57298
Additional Polypeptide Data & Comments
Reported size (kD)	1461 (aa); 165 (kD observed); 165 (kD predicted) (Basu et al., 1999)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0025458
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, core (IPR000719) Serine/threonine protein kinase, active site (IPR008271) Protein kinase-like (IPR011009) Mad3/BUB1 homology region 1 (IPR013212) Mitotic checkpoint serine/threonine protein kinase, Bub1 (IPR015661) Protein kinase ATP binding, conserved site (IPR017441) Serine/threonine protein kinase-related (IPR017442)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA263689   AA438552   AA441296   AA538805   AA539566   AA820292   AA820408   AA941084   AA942348   AA952281   AA979700   AC009847   AE003784 AAF57298   AF080399 AAC32190   AF132565 AAD27864   AI455383   AI456003   AI514378   AI519744   AI520083   AQ025781   BE977679   BG638777   BI373082   BP551408   BP551409   CO290007   CO307858   CO316090   CO321484   CO322184   CO327123   CO328433   CZ466426   DN154273   EC052327   EC054841   EC055041   EC056081   EC056537   EC062937   EC064158   EC212587   EC219141   EC226512   EC226810   EC231558   EC240560   EC243802   LD35813
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	O76755 Q7KN56 A1Z6I7
Maps to	FBcl0297629 (FlyBase, 1992-)
Does NOT map to
Identified with	LD18419 LD06986 (Basu et al., 1999) LD24007 (Spradling et al., 1999) LD23835 (Spradling et al., 1999, Tsang, 1999.3.2, BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 11596
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term	kinetochore (Basu et al., 1999)
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AI456003
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0025458 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0025458 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0025458 FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for BubR1 Stage(s) 4-6 Stage(s) 7-8 Stage(s) 9-10 Stage(s) 11-12
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele semi-viable BubR1Rev1 lethal | recessive BubR1k03113 BubR1k06109 Other Phenotypes Allele meiotic cell cycle defective | recessive BubR1unspecified mitotic cell cycle defective BubR1Rev1 BubR1dsRNA.cGa BubR1k03113 BubR1k06109 BubR1unspecified mitotic cell cycle defective (with BubR1k06109) BubR1Rev1 mitotic cell cycle defective (with BubR1Rev1) BubR1k06109 mitotic cell cycle defective | recessive BubR1k03113 BubR1k06109 mitotic cell cycle defective | embryonic stage 2 BubR1Rev1 mitotic cell cycle defective | embryonic stage 3 BubR1Rev1 mitotic cell cycle defective | embryonic stage 4 BubR1Rev1 Sterility Allele female sterile BubR1Rev1 Phenotype manifest in Allele neuroblast BubR1Rev1 BubR1k06109 condensed chromosome BubR1Rev1 BubR1k06109 nucleus BubR1Rev1 spindle BubR1Rev1 neuroblast (with BubR1k06109) BubR1Rev1 condensed chromosome (with BubR1k06109) BubR1Rev1 mitotic cell cycle BubR1k03113 BubR1k06109 BubR1unspecified mitotic anaphase BubR1k03113 BubR1k06109 BubR1unspecified mitotic anaphase & nuclear chromosome BubR1k03113 BubR1k06109 imaginal disc BubR1k03113 neuroblast (with BubR1Rev1) BubR1k06109 condensed chromosome (with BubR1Rev1) BubR1k06109 meiotic cell cycle | male BubR1unspecified onion stage spermatid BubR1unspecified onion stage spermatid & nucleus BubR1unspecified mitosis & nuclear chromosome BubR1unspecified S2 cell-line BubR1dsRNA.cGa kinetochore microtubule BubR1dsRNA.cGa
Classical Alleles ( 5 )
For All Classical Alleles Show
Allele of BubR1 Class Mutagen Stocks Known lesion BubR1Rev1 hypomorph 0 Yes BubR1c00394 piggyBac transposase 1 -- BubR1k03113 hypomorph P-element activity 2 Yes BubR1k06109 hypomorph P-element activity 1 Yes BubR1unspecified 0 --
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of BubR1 Class Mutagen Stocks Known lesion BubR1DN.Scer\UAS antimorph in vitro construct | regulatory fusion in vitro construct | deletion 3 Yes BubR1GD10778 in vitro construct | RNAi in vitro construct | regulatory fusion 1 Yes BubR1Scer\UAS.Exel in vitro construct | regulatory fusion 3 Yes BubR1T:Avic\GFP-S65T in vitro construct | coding region fusion 0 Yes BubR1T:Disc\RFP-mRFP in vitro construct | coding region fusion 0 Yes BubR1dsRNA.cGa in vitro construct | RNAi 0 --
Aneuploid Aberrations
Useful deficiency	Df(2R)nap1
Useful duplication	Dp(2;2)BG
Disrupted in	Df(2R)Exel7085 (Ryder, 2004.4.26) Df(2R)NC13 (Myster and Peifer, 2003.9.11, Myster et al., 2004) Df(2R)NC228 (Myster and Peifer, 2003.9.11, Myster et al., 2004) Df(2R)nap1 (Basu et al., 1999, Spradling et al., 1999, Myster et al., 2004, BDGP Project Members, 1994-1999) Df(2R)nap2 (Basu et al., 1999, Spradling et al., 1999, BDGP Project Members, 1994-1999)
Not disrupted in	Df(2R)244 (Myster et al., 2004) Df(2R)247 (Myster et al., 2004) Df(2R)Dark2 (Myster et al., 2004) Df(2R)M41A10 (Myster et al., 2004, Myster et al., 2004) Df(2R)M41A2 (Myster et al., 2004, Myster et al., 2004) Df(2R)M41A4 (Spradling et al., 1999, BDGP Project Members, 1994-1999) Df(2R)M41A8 (Myster et al., 2004, Myster et al., 2004) Df(2R)NC109 (Myster et al., 2004) Df(2R)NC1198 (Myster et al., 2004) Df(2R)NC125 (Myster et al., 2004) Df(2R)NC138 (Myster et al., 2004) Df(2R)NC167 (Myster et al., 2004) Df(2R)NC168 (Myster et al., 2004) Df(2R)NC191 (Myster et al., 2004) Df(2R)NC195 (Myster et al., 2004) Df(2R)NC202 (Myster et al., 2004) Df(2R)NC207 (Myster et al., 2004) Df(2R)NC218 (Myster et al., 2004) Df(2R)NC219 (Myster et al., 2004) Df(2R)NC32 (Myster et al., 2004) Df(2R)NC50 (Myster et al., 2004) Df(2R)NC68 (Myster et al., 2004) Df(2R)NC73 (Myster et al., 2004) Df(2R)NC89 (Myster et al., 2004) Df(2R)NC9 (Myster et al., 2004) Df(2R)NC98 (Myster et al., 2004) Df(2R)Nipped-D (Myster et al., 2004, Myster et al., 2004)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{UAS-BubR1.DN} NA P{UAS-BubR1.Exel} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{PB}BubR1c00394 NA insertion of enhancer trap P{lacW}BubR1k03113 No P{lacW}BubR1k06109 No
Related Comments
	Please look at the allele reports for the complete phenotype data Mutants isolated in a screen of the second chromosome identifying genes affecting disc morphology. (Roch et al., 1998) When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, aneuploidy, an increase in mitotic index, a decrease in the ratio of cells in prometaphase and metaphase versus the total number of mitotic cells, a whole range of mitotic abnormalities, central spindle defects, chromosome abnormalities, and lagging chromatids are seen. (Bettencourt-Dias et al., 2004) Loss of function mutations in BubR1 cause severe mitotic abnormalities consistent with accelerated transit through metaphase. (Basu et al., 1999) dsRNA against this gene has been used to treat SL2 cells to study the effect of depletion of BubR1 on the spindle checkpoint. (Muller et al., 2006) RNAi screen using dsRNA made from templates generated with primers directed against this gene results in chromosome misalignment on the metaphase spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007)
Gene Ontology: Function, Process & Cellular Component ( 12 )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441, InterPro:IPR017442 (FlyBase Curators et al., 2004-)
protein kinase activity non-traceable author statement (Morrison et al., 2000)
protein serine/threonine kinase activity inferred from sequence or structural similarity (Spradling et al., 1999) non-traceable author statement (Basu, 1998.7.24) inferred from electronic annotation with InterPro:IPR008271 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
mitotic cell cycle spindle assembly checkpoint inferred from mutant phenotype (Basu et al., 1999) inferred from sequence or structural similarity (Spradling et al., 1999) traceable author statement (Lee and Orr-Weaver, 2003) inferred from direct assay (Logarinho et al., 2004)
mitotic spindle organization and biogenesis inferred from mutant phenotype (Goshima et al., 2007)
protein amino acid phosphorylation non-traceable author statement (Morrison et al., 2000) inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR008271, InterPro:IPR017441, InterPro:IPR017442 (FlyBase Curators et al., 2004-)
regulation of exit from mitosis inferred from genetic interaction with FLYBASE:dup; FB:FBgn0000996 AND inferred from mutant phenotype (Garner et al., 2001)
spindle checkpoint inferred from mutant phenotype (Perez-Mongiovi et al., 2005)
syncytial blastoderm mitotic cell cycle inferred from mutant phenotype (Perez-Mongiovi et al., 2005)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Buffin et al., 2005)
kinetochore traceable author statement (Basu et al., 1998) inferred from direct assay (Buffin et al., 2005)
outer kinetochore of condensed chromosome non-traceable author statement (Blower and Karpen, 2001)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions BubR1 allele Gene References Bub1k03113 asp (Basto et al., 2000) dup (Garner et al., 2001) Bub1k06109 polo (Donaldson et al., 2001) unspecified Cdc27 (Deak, 1999.9.15)
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 61428
Orthologs
Genome-wide drosophilid orthologs	Dana\GF13829 Dere\GG10842 Dgri\GH20410 Dmoj\GI19597 Dper\GL17789 Dpse\GA20621 Dsec\GM16494 Dsim\GD10345 Dvir\GJ18402 Dwil\GK21755 Dyak\GE19436
Curated drosophilid orthologs	Dpse\GA20621
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000008371 Anopheles gambiae (Ensembl) ENSCAFP00000013122 Canis familiaris (Ensembl) ENSCINP00000019856 Ciona intestinalis (Ensembl) NEWSINFRUP00000165671 Takifugu rubripes (Ensembl) ENSP00000287598 Homo sapiens (Ensembl) ENSMMUP00000021937 Macaca mulatta (Ensembl) ENSPTRP00000011804 Pan troglodytes (Ensembl) GSTENP00007581001 Tetraodon nigroviridis (Ensembl) ENSXETP00000000565 Xenopus tropicalis (Ensembl) Q7XIR5 Oryza sativa (Gramene) AT2G33560.1 Arabidopsis thaliana (Arabidopsis) WBGene00033080 Caenorhabditis briggsae (Wormbase) WBGene00040583 Caenorhabditis briggsae (Wormbase) MGI:1100510 Mus musculus (Informatics) SPCC1795.01c Schizosaccharomyces pombe (Sanger)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 11 )
Harvard	- PBac{PB}BubR1c00394
VDRC	v26109 w1118; P{GD10778}v26109/CyO
Blooming