Gene Dmel\Flo

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General Information

Symbol

Dmel\Flo

Species

D. melanogaster

Name

flotillin

Annotation symbol

CG8200

Feature type

protein_coding_gene

FlyBase ID

FBgn0024754

Created / Updated

2003-12-02/2003-12-02

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

52A13-52A13

Sequence location

2R:11,453,772..11,456,907 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene flotillin is referred to in FlyBase by the symbol Flo (CG8200, FBgn0024754). It has the cytological map location 52A13. Its sequence location is 2R:11453772..11456907. Its molecular function is described as structural molecule activity. The biological processes in which it is involved are not known. 3 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

52A13-52A13

Limits computationally determined from genome sequence between P{lacW}Vha36^k07207 and P{lacW}l(2)k05713^k05713

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

52B1-52B2

(determined by in situ hybridisation)

(Galbiati et al., 1998)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Flo for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Flo-RA

FBtr0087351

NM_058010

1820

426

Flo-RB

FBtr0087352

NM_166102

1832

430

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Flo-PA

FBpp0086484

47.1

426

5.35

NP_477358

AAF58120

Flo-PB

FBpp0086485

47.5

430

5.35

NP_725476

AAF58121

Additional Polypeptide Data & Comments

Reported size (kD)

426 (aa); 47 (kD predicted)

(Galbiati et al., 1998)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0024754

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Band 7 protein (IPR001107)

Flotillin (IPR004851)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

O61491

UniProtKB/TrEMBL

Maps to

DS04974

(Galbiati et al., 1998)

DS08592

(FlyBase, 1992-)

Does NOT map to

Identified with

CK02126

(Galbiati et al., 1998, Kopczynski et al., 1998)

SD10657

(BDGP Project Members, 2000-)

Dm0794

(Galbiati et al., 1998)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

12390

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

embryonic stage | stage >=12

ventral nerve cord

(Galbiati et al., 1998)

embryonic stage | stage >=12

embryonic brain

(Galbiati et al., 1998)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI543762

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0024754

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0024754

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0024754

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for Flo

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for Flo

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 2 )

For All Classical Alleles Show

Allele of Flo	Class	Mutagen	Stocks	Known lesion
Flo^KG06308		P-element activity	1	--
Flo^e02554		piggyBac transposase	1	--

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Flo	Class	Mutagen	Stocks	Known lesion
Flo^GD13839		in vitro construct \| RNAi in vitro construct \| regulatory fusion	3	Yes

Aneuploid Aberrations

Useful deficiency

Df(2R)WMG

Useful duplication

Dp(2;2)SMG45

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD13839}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{RB}Flo^e02554

P{SUPor-P}Flo^KG06308

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 3 )

Molecular Function

CV term

Evidence

References

structural molecule activity

inferred from expression pattern

(Galbiati et al., 1998)

Biological Process

CV term

Evidence

References

Cellular Component

CV term

Evidence

References

flotillin complex

non-traceable author statement

(Galbiati et al., 1998)

non-traceable author statement

(Swiss-Prot Project Members, 2000.10.1)

inferred from electronic annotation with InterPro:IPR004851

(FlyBase Curators et al., 2004-)

integral to membrane

inferred from electronic annotation with InterPro:IPR004851

(FlyBase Curators et al., 2004-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Flo allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

62460

Drosophila PIMRider - The Drosophila Protein Interaction map

•

521587

Orthologs

Genome-wide drosophilid orthologs

Dana\GF22637

Dere\GG22345

Dgri\GH20287

Dmoj\GI19457

Dper\GL11508

Dpse\GA20892

Dsec\GM20133

Dsim\GD25610

Dvir\GJ21208

Dwil\GK21951

Dyak\GE14146

Curated drosophilid orthologs

Dpse\GA20892

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000009431
Anopheles gambiae (Ensembl)
ENSBTAP00000013135
Bos taurus (Ensembl)
ENSCAFP00000000650
Canis familiaris (Ensembl)
NEWSINFRUP00000151851
Takifugu rubripes (Ensembl)
ENSP00000259846
Homo sapiens (Ensembl)
ENSMODP00000019794
Monodelphis domestica (Ensembl)
ENSPTRP00000030607
Pan troglodytes (Ensembl)
ENSRNOP00000001098
Rattus norvegicus (Ensembl)
GSTENP00015301001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000051960
Xenopus tropicalis (Ensembl)
Q8LNW6
Oryza sativa (Gramene)
AT5G25250.1
Arabidopsis thaliana (Arabidopsis)
AT5G25260.1
Arabidopsis thaliana (Arabidopsis)
ZDB-GENE-000210-36
Danio rerio (Zfin)
MGI:1100500
Mus musculus (Informatics)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 5 )

Harvard

PBac{RB}Flo^e02554

VDRC

v42129

w¹¹¹⁸; P{GD13839}v42129

v42130

w¹¹¹⁸; P{GD13839}v42130

v45828

w¹¹¹⁸; P{GD13839}v45828

Bloomington

14481

y¹ w^67c23; P{SUPor-P}Flo^KG06308

Genomic Clones ( 1 )

BACR48C01

cDNA Clones ( 40 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones