Gene Dmel\dnt

37D2-37D2

(determined by in situ hybridisation)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\dnt for information on other features

Comments on Gene Model

Transposon inserted in intron

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

dnt-RA

FBtr0081224

NM_057993

2629

584

Additional Transcript Data & Comments

Reported size (kB)

2.6 (longest cDNA)

2.7 (northern blot)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

dnt-PA

FBpp0080765

64.9

584

6.96

NP_477341

AAF53783

Additional Polypeptide Data & Comments

Reported size (kD)

561 (aa)

565 (aa)

Comments

External Data

(BDGP Project Members, 2000-)

PANTHER - Protein classification by function, families, and pathways

•

FBgn0024245

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Protein kinase, core (IPR000719)

Tyrosine protein kinase (IPR001245)

WIF domain (IPR003306)

Tyrosine protein kinase, active site (IPR008266)

Protein kinase-like (IPR011009)

Protein kinase ATP binding, conserved site (IPR017441)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9V422

UniProtKB/TrEMBL

Q5BII5

Maps to

FBcl0297588

(FlyBase, 1992-)

Does NOT map to

Identified with

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

DEDB - Drosophila exon database: splicing graphs

•

8095

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

embryonic stage-larval stage

embryonic stage | blastoderm

embryonic stage | gastrulation

cephalic furrow

embryonic stage | late

imaginal precursor

embryonic stage | gastrulation

amnioproctodeal invagination

embryonic stage | stage >= 5

embryonic stage | stage 11

embryonic/larval tracheal pit | surrounding

embryonic stage | extended germ band

embryonic/larval tracheal pit | surrounding

embryonic stage | gastrulation-extended germ band

embryonic stage | gastrulation

ventral furrow

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI531640

External Data & Images

(Bettencourt-Dias et al., 2004)

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0024245

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0024245

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0024245

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 4 )

For All Classical Alleles Show

Allele of dnt	Mutagen	Stocks	Known lesion
dnt^DG04604	P-element activity	1	--
dnt^EP2158	P-element activity	1	--
dnt^c03766	piggyBac transposase	1	--
dnt^e00722	piggyBac transposase	1	--

Alleles Carried on Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Allele of dnt	Mutagen	Stocks	Known lesion
dnt^{Act5C.T:BPV-1\AU1}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes
dnt^GD14372	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
dnt^GD2539	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
dnt^GD43	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
dnt^{K345R.Act5C.T:BPV-1\AU1}	in vitro construct \| amino acid replacement	0	Yes
dnt^ap.ME4	in vitro construct \| regulatory fusion	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(2L)VA23

Disrupted in

Df(2L)Exel6043

(Ryder, 2004.4.26)

Df(2L)Exel7075

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD14372}

P{GD2539}

P{GD43}

characterization construct

P{ME4-dnt.O}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{PB}dnt^c03766

PBac{RB}dnt^e00722

P{wHy}dnt^DG04604

insertion of mobile activating element

P{EP}dnt^EP2158

Related Comments

Please look at the allele reports for the complete phenotype data

When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, a decrease in the ratio of cells in prometaphase and metaphase versus the total number of mitotic cells, a whole range of mitotic abnormalities, centrosome abnormalities, spindle abnormalities and chromosome abnormalities are seen.

Gene Ontology: Function, Process & Cellular Component ( 9 )

Molecular Function

CV term

Evidence

References

ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR001245, InterPro:IPR008266, InterPro:IPR017441

(FlyBase Curators et al., 2004-)

protein tyrosine kinase activity

non-traceable author statement

(Oates, 1998.2.14)

non-traceable author statement

(Savant-Bhonsale, 1999.1.26)

non-traceable author statement

(Morrison et al., 2000)

transmembrane receptor protein tyrosine kinase activity

inferred from genetic interaction with FLYBASE:drl; FB:FBgn0015380 AND inferred from sequence or structural similarity with UniProtKB:P34925

Biological Process

CV term

Evidence

References

axon guidance

inferred from mutant phenotype

muscle attachment

inferred from mutant phenotype

protein amino acid phosphorylation

inferred from sequence or structural similarity with UniProtKB:P34925

non-traceable author statement

(Morrison et al., 2000)

signal transduction

inferred from mutant phenotype

Cellular Component

CV term

Evidence

References

integral to membrane

inferred from sequence or structural similarity with UniProtKB:P34925

plasma membrane

inferred from sequence or structural similarity

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

dnt allele

Gene

References

External Data

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

61188

Orthologs

Genome-wide drosophilid orthologs

Dana\GF15200

Dere\GG21639

Dgri\GH13473

Dmoj\GI17225

Dper\GL21115

Dpse\GA14553

Dsec\GM17017

Dsim\GD21767

Dvir\GJ17984

Dwil\GK23845

Dyak\GE12658

Curated drosophilid orthologs

Dpse\GA14553

y¹ w^67c23; P{wHy}dnt^DG04604

InParanoid orthologs - Eukaryotic orthologs

•

ENSBTAP00000022607
Bos taurus (Ensembl)
ENSCAFP00000010007
Canis familiaris (Ensembl)
ENSCINP00000018455
Ciona intestinalis (Ensembl)
ENSGALP00000010474
Gallus gallus (Ensembl)
ENSP00000296084
Homo sapiens (Ensembl)
ENSMMUP00000014767
Macaca mulatta (Ensembl)
ENSMODP00000023074
Monodelphis domestica (Ensembl)
ENSPTRP00000026572
Pan troglodytes (Ensembl)
ENSRNOP00000046731
Rattus norvegicus (Ensembl)
ENSXETP00000025844
Xenopus tropicalis (Ensembl)
WBGene00034859
Caenorhabditis briggsae (Wormbase)
MGI:101766
Mus musculus (Informatics)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 9 )

v6547

w¹¹¹⁸; P{GD43}v6547

v6548

w¹¹¹⁸; P{GD43}v6548

v27056

w¹¹¹⁸; P{GD14372}v27056

v27057

w¹¹¹⁸; P{GD14372}v27057

v8002

w¹¹¹⁸; P{GD2539}v8002/TM3

Bloomington

20385

Genomic Clones ( 2 )

cDNA Clones ( 17 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0024245

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0024245

Antibody Information

Other Information

Discoverer

Etymology

Etymology: the gene is named "doughnut" after part of the embryonic expression pattern, which is in doughnut-shaped rings surrounding the developing tracheal pits immediately prior to their invagination.