Gene Dmel\mRpL16

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General Information

Symbol

Dmel\mRpL16

Species

D. melanogaster

Name

mitochondrial ribosomal protein L16

Annotation symbol

CG3109

Feature type

protein_coding_gene

FlyBase ID

FBgn0023519

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

2B14-2B14

Sequence location

X:1,784,332..1,785,313 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene mitochondrial ribosomal protein L16 is referred to in FlyBase by the symbol mRpL16 (CG3109, FBgn0023519). It has the cytological map location 2B14. Its sequence location is X:1784332..1785313. Its molecular function is described as structural constituent of ribosome. It is involved in the biological process translation. 7 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

2B14-2B14

Limits computationally determined from genome sequence between P{EP}EP1444&P{EP}CG14818^EP1190 and P{EP}CG3600^EP1232

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

2B-2B

(Riggleman et al., 1989)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Overall orientation not stated: anon-2Ba? trr? mRpL16+ arm- mRpL16+ arm- anon-2Be+ anon-2Bc?

(Riggleman et al., 1989)

Overall orientation not stated: anon-2Ba? trr- mRpL16+ arm- mRpL16+ arm- anon-2Be+

(Sedkov et al., 1999)

Overall orientation not stated: trr? mRpL16? arm?

(Gatt and Ashburner, 2000.2.3)

Gene Model & Products

Please see the GBrowse view of Dmel\mRpL16 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

mRpL16-RA

FBtr0070314

NM_080302

831

243

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

mRpL16-PA

FBpp0070301

28.0

243

10.79

NP_525041

AAF45685

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0023519

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Ribosomal protein L16 (IPR000114)

Ribosomal protein L10e/L16 (IPR016180)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

bs02f04

(Oliver, 2001.8.16)

GH05976

(Benos et al., 2001, Oliver, 2001.8.16)

LD21404

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0023519

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0023519

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0023519

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for mRpL16

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for mRpL16

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 4 )

For All Classical Alleles Show

Allele of mRpL16	Mutagen	Stocks	Known lesion
mRpL16^A	ethyl methanesulfonate	0	--
mRpL16^B	ethyl methanesulfonate	1	--
mRpL16^C	ethyl methanesulfonate	1	--
mRpL16^D	ethyl methanesulfonate	0	--

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of mRpL16	Mutagen	Stocks	Known lesion
mRpL16^GD6376	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
mRpL16^t12	in vitro construct \| genomic fragment	1	Yes
mRpL16^t8	in vitro construct \| genomic fragment	0	Yes

Aneuploid Aberrations

Useful deficiency

T(1;3)143-3

Useful duplication

Dp(1;1)M105.6

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD6376}

Insertions

Type of insertions

Name

Expression data

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 3 )

Molecular Function

CV term

Evidence

References

structural constituent of ribosome

inferred from sequence or structural similarity with EMBL:AB049642

(Suzuki et al., 2001)

inferred from sequence or structural similarity

(EDGP Project Members, 1994-)

Biological Process

CV term

Evidence

References

translation

inferred from sequence or structural similarity with EMBL:AB049642

(Suzuki et al., 2001)

inferred from sequence or structural similarity with EMBL:Z35663

(FlyBase, 1992-)

Cellular Component

CV term

Evidence

References

mitochondrial large ribosomal subunit

inferred from sequence or structural similarity with EMBL:AB049642

(Suzuki et al., 2001)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

mRpL16 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

57696

Orthologs

Genome-wide drosophilid orthologs

Dana\GF19450

Dere\GG12889

Dgri\GH17672

Dmoj\GI21490

Dper\GL18214

Dpse\GA16000

Dsec\GM19177

Dsim\GD16576

Dvir\GJ19031

Dwil\GK16222

Curated drosophilid orthologs

Dpse\GA16000

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000011467
Anopheles gambiae (Ensembl)
ENSBTAP00000028236
Bos taurus (Ensembl)
ENSCAFP00000015168
Canis familiaris (Ensembl)
ENSGALP00000008312
Gallus gallus (Ensembl)
ENSP00000300151
Homo sapiens (Ensembl)
ENSMMUP00000029383
Macaca mulatta (Ensembl)
ENSMODP00000025272
Monodelphis domestica (Ensembl)
ENSPTRP00000006376
Pan troglodytes (Ensembl)
ENSRNOP00000028517
Rattus norvegicus (Ensembl)
GSTENP00017920001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000052237
Xenopus tropicalis (Ensembl)
WBGene00037504
Caenorhabditis briggsae (Wormbase)
WBGene00011412
Caenorhabditis elegans (Wormbase)
ZDB-CDNA-041122-10
Danio rerio (Zfin)
ZDB-GENE-041122-1
Danio rerio (Zfin)
MGI:2137219
Mus musculus (Informatics)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 4 )

VDRC

v14528

w¹¹¹⁸; P{GD6376}v14528

v14529

w¹¹¹⁸; P{GD6376}v14529

Bloomington

9920

y¹ mRpL16^B w^67c23/FM7a

9921

C(1)DX, y¹ f¹/y¹ mRpL16^C w^67c23; P{trr^+t12}2/+

Genomic Clones ( 1 )

BACR33I18

cDNA Clones ( 8 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

LD21404

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0023519

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0023519

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: EG:63B12.2 anon-2Bd

(Gatt and Ashburner, 2000.2.3)

Additional comments

Comments About Role

Comments About Molecular Function

Other Comments

Transcription unit identified during molecular analysis of the arm region.

(Riggleman et al., 1989)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_080302

RefSeq (Proteins)

•

NP_525041

DDBJ / EMBL / GenBank

•

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Ribosomal protein L16 (IPR000114)

Ribosomal protein L10e/L16 (IPR016180)

Linkouts

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for mRpL16

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

57696

DEDB - Drosophila exon database: splicing graphs

•

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0023519

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0023519

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0023519

FlyExpress - Embryonic expression images (BDGP data)

•

FBgn0023519

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0023519

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0023519

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0023519

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000011467
Anopheles gambiae (Ensembl)
ENSBTAP00000028236
Bos taurus (Ensembl)
ENSCAFP00000015168
Canis familiaris (Ensembl)
ENSGALP00000008312
Gallus gallus (Ensembl)
ENSP00000300151
Homo sapiens (Ensembl)
ENSMMUP00000029383
Macaca mulatta (Ensembl)
ENSMODP00000025272
Monodelphis domestica (Ensembl)
ENSPTRP00000006376
Pan troglodytes (Ensembl)
ENSRNOP00000028517
Rattus norvegicus (Ensembl)
GSTENP00017920001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000052237
Xenopus tropicalis (Ensembl)
WBGene00037504
Caenorhabditis briggsae (Wormbase)
WBGene00011412
Caenorhabditis elegans (Wormbase)
ZDB-CDNA-041122-10
Danio rerio (Zfin)
ZDB-GENE-041122-1
Danio rerio (Zfin)
MGI:2137219
Mus musculus (Informatics)

PANTHER - Protein classification by function, families, and pathways

•

FBgn0023519

Synonyms & Secondary IDs ( 8 )

Reported As

Symbol Synonym

anon-2Bd

(Benos et al., 2001, )

CG3109

(Benos, 2000.12.13, )

D-15

(Sedkov et al., 1999, Riggleman et al., 1989)

D15

(Sedkov, 2007.3.20)

EG:63B12.2

(Benos et al., 2001, Gatt and Ashburner, 2000.2.3, )

L16

(Suzuki et al., 2001)

mRpL16

(Deng and Meller, 2006, Sedkov, 2007.3.20, Furuhashi et al., 2006)

Name Synonym

mitochondrial ribosomal protein L16

(Suzuki et al., 2001)

Secondary FlyBase IDs

FBgn0019893

References ( 18 )

Research paper

Deng and Meller, 2006, Genetics 174(4): 1859--1866: roX RNAs are required for increased expression of X-linked genes in Drosophila melanogaster males. [FBrf0195031]
Furuhashi et al., 2006, Development 133(22): 4475--4483: DNA supercoiling factor contributes to dosage compensation in Drosophila. [FBrf0193355]
Hamada et al., 2005, Genes Dev. 19(19): 2289--2294: Global regulation of X chromosomal genes by the MSL complex in Drosophila melanogaster. [FBrf0190545]
Straub et al., 2005, Genes Dev. 19(19): 2284--2288: The Drosophila MSL complex activates the transcription of target genes. [FBrf0190565]
Benos et al., 2001, Genome Res. 11(5): 710--730: From First Base: the sequence of the tip of the X chromosome of Drosophila melanogaster, a comparison of two sequencing strategies. [FBrf0135823]
Suzuki et al., 2001, J. Biol. Chem. 276(24): 21724--21736: Structural compensation for the deficit of rRNA with proteins in the mammalian mitochondrial ribosome. Systematic analysis of protein components of the large ribosomal subunit from mammalian mitochondria. [FBrf0136752]
Sedkov et al., 1999, Mech. Dev. 82(1,2): 171--179: Molecular genetic analysis of the Drosophila trithorax-related gene which encodes a novel SET domain protein. [FBrf0108438]
Riggleman et al., 1989, Genes Dev. 3: 96--113: Molecular analysis of the armadillo locus: uniformly distributed transcripts and a protein with novel internal repeats are associated with a Drosophila segment polarity gene. [FBrf0049836]

Personal communication to FlyBase

Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
Sedkov, 2007.3.20, mRpL16 alleles and P{trr[+t12]}2 insertion.: mRpL16 alleles and P{trr[+t12]}2 insertion. [FBrf0198737]
Oliver, 2001.8.16, File from B. Oliver containing information on ESTs expressed in the Drosophila testis, described in Andrews et al., 2000, Genome Res. 10(12): 2030-2043 (FBrf0132348).: File from B. Oliver containing information on ESTs expressed in the Drosophila testis, described in Andrews et al., 2000, Genome Res. 10(12): 2030-2043 (FBrf0132348). [FBrf0137492]
Benos, 2000.12.13, FlyBase error report on Wed Dec 13 14:35:56 2000.: FlyBase error report on Wed Dec 13 14:35:56 2000. [FBrf0133222]
Gatt and Ashburner, 2000.2.3, Gene merges in X tip with a pc.: Gene merges in X tip with a pc. [FBrf0125090]

FlyBase analysis

FlyBase, 1992-, FlyBase curation: FlyBase curation. [FBrf0105495]

Computer file

BDGP Project Members, 2000-, Berkeley Drosophila Genome Project.: Berkeley Drosophila Genome Project. [FBrf0125078]
EDGP Project Members, 1994-, European Drosophila Genome Mapping Project.: European Drosophila Genome Mapping Project. [FBrf0071734]

Curated genome annotation

Ashburner, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126651]

Automatic genome annotation

FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126705]