Gene Dmel\CG3573

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General Information

Symbol

Dmel\CG3573

Species

D. melanogaster

Name

CG3573

Annotation symbol

CG3573

Feature type

protein_coding_gene

FlyBase ID

FBgn0023508

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

2B16-2B16

Sequence location

X:1,817,038..1,821,555 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol CG3573 (FBgn0023508). It has the cytological map location 2B16. Its sequence location is X:1817038..1821555. Its molecular function is described as: inositol-polyphosphate 5-phosphatase activity; inositol trisphosphate phosphatase activity; inositol or phosphatidylinositol phosphatase activity. It is involved in the biological processes: dephosphorylation; signal transduction. 2 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

2B16-2B16

Limits computationally determined from genome sequence between P{EP}EP1444&P{EP}CG14818^EP1190 and P{EP}CG3600^EP1232

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\CG3573 for information on other features

Comments on Gene Model

DGC clone appears problematic (LP11751): incomplete CDS

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

CG3573-RA

FBtr0070317

NM_130606

3662

850

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

CG3573-PA

FBpp0070304

97.5

850

5.67

NP_569962

AAF45696

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0023508

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

RhoGAP (IPR000198)

Inositol polyphosphate related phosphatase (IPR000300)

Endonuclease/exonuclease/phosphatase (IPR005135)

Rho GTPase activation protein (IPR008936)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

GH18456

(Benos et al., 2001)

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI114232

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0023508

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0023508

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

CG3573^EY15890

Sterility

Allele

fertile

CG3573^EY15890

Phenotype manifest in

Allele

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of CG3573	Class	Mutagen	Stocks	Known lesion
CG3573^EY15890		P-element activity	1	--

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of CG3573	Class	Mutagen	Stocks	Known lesion
CG3573^GD11016		in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes

Aneuploid Aberrations

Useful deficiency

T(1;3)143-3

Useful duplication

Dp(1;1)M105.6

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD11016}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EPgy2}CG3573^EY15890

Related Comments

Please look at the allele reports for the complete phenotype data

dsRNA made from templates generated with primers directed against CG3573 that is transfected into S2 treated with Listeria monocytogenes reveals CG3573 to be involved in Listeria monocytogenes entry, intracellular growth, and vacuolar escape. Treatment with Sbf dsRNA leads to an approximate 50% decrease in bacterial entry, and an approximate 35% decrease in vacuolar escape.

(Cheng et al., 2005)

Gene Ontology: Function, Process & Cellular Component ( 6 )

Molecular Function

CV term

Evidence

References

inositol or phosphatidylinositol phosphatase activity

inferred from electronic annotation with InterPro:IPR000300

(FlyBase Curators et al., 2004-)

inositol trisphosphate phosphatase activity

non-traceable author statement

(Morrison et al., 2000)

inositol-polyphosphate 5-phosphatase activity

inferred from sequence or structural similarity

(EDGP Project Members, 1994-)

Biological Process

CV term

Evidence

References

dephosphorylation

non-traceable author statement

(Morrison et al., 2000)

signal transduction

inferred from electronic annotation with InterPro:IPR000198, InterPro:IPR008936

(FlyBase Curators et al., 2004-)

Cellular Component

CV term

Evidence

References

intracellular

inferred from electronic annotation with InterPro:IPR000198, InterPro:IPR008936

(FlyBase Curators et al., 2004-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

CG3573 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

57703

Drosophila PIMRider - The Drosophila Protein Interaction map

•

522127

Orthologs

Genome-wide drosophilid orthologs

Dper\GL18219

Curated drosophilid orthologs

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000026404
Anopheles gambiae (Ensembl)
ENSBTAP00000033176
Bos taurus (Ensembl)
ENSCAFP00000004807
Canis familiaris (Ensembl)
ENSCINP00000008342
Ciona intestinalis (Ensembl)
NEWSINFRUP00000157508
Takifugu rubripes (Ensembl)
ENSGALP00000002441
Gallus gallus (Ensembl)
ENSP00000287254
Homo sapiens (Ensembl)
ENSMMUP00000011570
Macaca mulatta (Ensembl)
ENSMODP00000022118
Monodelphis domestica (Ensembl)
ENSPTRP00000043267
Pan troglodytes (Ensembl)
ENSRNOP00000010885
Rattus norvegicus (Ensembl)
GSTENP00029323001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000031592
Xenopus tropicalis (Ensembl)
AT1G05470.1
Arabidopsis thaliana (Arabidopsis)
AT2G32010.1
Arabidopsis thaliana (Arabidopsis)
WBGene00026596
Caenorhabditis briggsae (Wormbase)
WBGene00007620
Caenorhabditis elegans (Wormbase)
DDB0191151
Dictyostelium discoideum (Dictybase)
MGI:103257
Mus musculus (Informatics)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 2 )

VDRC

v34649

w¹¹¹⁸; P{GD11016}v34649

Bloomington

21170

y¹ P{EPgy2}CG3573^EY15890 w^67c23

Genomic Clones ( 2 )

cDNA Clones ( 17 )

cDNA Clones, Fully Sequenced

BDGP DGC clones

LD39196

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0023508

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0023508

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Comments About Role

Comments About Molecular Function

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

One of 42 Drosophila genes identified as being most likely to reveal molecular and cellular mechanisms of nervous system development or plasticity relevant to human Mental Retardation disorders.

(Inlow and Restifo, 2004)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_130606

RefSeq (Proteins)

•

NP_569962

DDBJ / EMBL / GenBank

•

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

RhoGAP (IPR000198)

Inositol polyphosphate related phosphatase (IPR000300)

Endonuclease/exonuclease/phosphatase (IPR005135)

Rho GTPase activation protein (IPR008936)

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

57703

DEDB - Drosophila exon database: splicing graphs

•

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0023508

Drosophila PIMRider - The Drosophila Protein Interaction map

•

522127

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0023508

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0023508

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0023508

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0023508

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000026404
Anopheles gambiae (Ensembl)
ENSBTAP00000033176
Bos taurus (Ensembl)
ENSCAFP00000004807
Canis familiaris (Ensembl)
ENSCINP00000008342
Ciona intestinalis (Ensembl)
NEWSINFRUP00000157508
Takifugu rubripes (Ensembl)
ENSGALP00000002441
Gallus gallus (Ensembl)
ENSP00000287254
Homo sapiens (Ensembl)
ENSMMUP00000011570
Macaca mulatta (Ensembl)
ENSMODP00000022118
Monodelphis domestica (Ensembl)
ENSPTRP00000043267
Pan troglodytes (Ensembl)
ENSRNOP00000010885
Rattus norvegicus (Ensembl)
GSTENP00029323001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000031592
Xenopus tropicalis (Ensembl)
AT1G05470.1
Arabidopsis thaliana (Arabidopsis)
AT2G32010.1
Arabidopsis thaliana (Arabidopsis)
WBGene00026596
Caenorhabditis briggsae (Wormbase)
WBGene00007620
Caenorhabditis elegans (Wormbase)
DDB0191151
Dictyostelium discoideum (Dictybase)
MGI:103257
Mus musculus (Informatics)

PANTHER - Protein classification by function, families, and pathways

•

FBgn0023508

University of Chicago Dev. Expression

•

FBgn0023508

Synonyms & Secondary IDs ( 3 )

Reported As

Symbol Synonym

CG3573

EG:86E4.5

(Inlow and Restifo, 2004, Bernards, 2003, Driscoll and Gerstbrein, 2003, Benos et al., 2001, Giot et al., 2003, )

Name Synonym

CG3573

inositol 5-phosphatase

(Majerus et al., 1999)

Secondary FlyBase IDs

References ( 20 )

Research paper

Cheng et al., 2005, Proc. Natl. Acad. Sci. USA 102(38): 13646--13651: Use of RNA interference in Drosophila S2 cells to identify host pathways controlling compartmentalization of an intracellular pathogen. [FBrf0188255]
Straub et al., 2005, Genes Dev. 19(19): 2284--2288: The Drosophila MSL complex activates the transcription of target genes. [FBrf0190565]
Inlow and Restifo, 2004, Genetics 166(2): 835--881: Molecular and comparative genetics of mental retardation. [FBrf0174714]
Arbeitman et al., 2002, Science 297(5590): 2270--2275: Gene expression during the life cycle of Drosophila melanogaster. [FBrf0152339]
Benos et al., 2001, Genome Res. 11(5): 710--730: From First Base: the sequence of the tip of the X chromosome of Drosophila melanogaster, a comparison of two sequencing strategies. [FBrf0135823]

Supplementary material

Driscoll and Gerstbrein, 2003, Nature Rev. Genet. 4(3):: Dying for a cause: invertebrate genetics takes on human neurodegeneration. [FBrf0159901]
Giot et al., 2003, Science 302(5651):: Tables S1 to S7. [FBrf0166452]
Kiger et al., 2003, J. Biol. 2(4):: Additional data file 1: The gene identity and primer sequences for dsRNAs used in the RNAi screens. [FBrf0174231]
Morrison et al., 2000, J. Cell Biol. 150(2):: Table S1. Drosophila protein kinases and phosphatases. [FBrf0132098]

Review

Bernards, 2003, Biochim. biophys. Acta 1603(2): 47--82: GAPs galore! A survey of putative Ras superfamily GTPase activating proteins in man and Drosophila. [FBrf0158741]
Majerus et al., 1999, J. Biol. Chem. 274(16): 10669--10672: The role of phosphatases in inositol signaling reactions. [FBrf0108400]

Personal communication to FlyBase

Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms.: Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]
Benos, 2000.12.13, FlyBase error report on Wed Dec 13 14:35:56 2000.: FlyBase error report on Wed Dec 13 14:35:56 2000. [FBrf0133222]

FlyBase analysis

FlyBase, 1992-, FlyBase curation: FlyBase curation. [FBrf0105495]

Computer file

Gene Disruption Project members, 2001-, [title not yet available]: [title not yet available] [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project.: Berkeley Drosophila Genome Project. [FBrf0125078]
EDGP Project Members, 1994-, European Drosophila Genome Mapping Project.: European Drosophila Genome Mapping Project. [FBrf0071734]

Curated genome annotation

Bixiong, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126692]

Automatic genome annotation

FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126705]