Gene Dmel\eIF4G

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General Information

Symbol

Dmel\eIF4G

Species

D. melanogaster

Name

eukaryotic translation initiation factor 4G

Annotation symbol

CG10811

Feature type

protein_coding_gene

FlyBase ID

FBgn0023213

Created / Updated

2003-12-02/2007-09-28

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

102C5-102C5

Sequence location

4:935,911..951,347 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene eukaryotic translation initiation factor 4G is referred to in FlyBase by the symbol eIF4G (CG10811, FBgn0023213). It has the cytological map location 102C5. Its sequence location is 4:935911..951347. Its molecular function is described as: translation initiation factor activity; protein binding. It is involved in the biological processes: translational initiation; mitotic spindle elongation; mitotic spindle organization and biogenesis; RNA metabolic process. 3 alleles are reported. No phenotypic data is available. It has 3 annotated transcripts and 3 annotated polypeptides.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

102C5-102C5

Limits computationally determined from genome sequence between P{SUPor-P}Asator^KG05051&P{SUPor-P}KG01424 and PBac{3HPy⁺}C024

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

102E-102E

(Hernandez, 1997.10.16)

102E-102E

(determined by in situ hybridisation)

(Hernandez et al., 1998)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\eIF4G for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

eIF4G-RA

FBtr0089243

NM_079901

5594

1666

eIF4G-RB

FBtr0112904

NM_001103382

6353

1919

eIF4G-RC

FBtr0289951

5824

1666

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

eIF4G-PA

FBpp0088303

183.9

1666

7.92

NP_524640

AAF59403

eIF4G-PB

FBpp0111817

211.1

1919

8.54

NP_001096852

ABV53593

eIF4G-PC

FBpp0288389

183.9

1666

7.92

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0023213

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

eIF4-gamma/eIF5/eIF2-epsilon (IPR003307)

MIF4G-like, type 3 (IPR003890)

Initiation factor eIF-4 gamma, MA3 (IPR003891)

MIF4-like, type 1/2/3 (IPR016021)

Armadillo-type fold (IPR016024)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

13646

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0023213

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0023213

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of eIF4G	Class	Mutagen	Stocks	Known lesion
eIF4G^e02087		piggyBac transposase	1	--

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of eIF4G	Class	Mutagen	Stocks	Known lesion
eIF4G^GD7098		in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
eIF4G^dsRNA.cHa		in vitro construct \| RNAi	0	Yes

Aneuploid Aberrations

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD7098}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{RB}eIF4G^e02087

Related Comments

Please look at the allele reports for the complete phenotype data

Drosophila initiation factor eIF-4F is composed of two subunits, one is encoded by eIF-4E and the other by eIF-4G, a 200kD protein. eIF-4G is required for the translation of endogenous mRNAs in cell-free systems made from Drosophila embryos, and can cross-link uncapped, but not m⁷G capped RNA. eIF-4E cross-links with m⁷G capped RNA.

(Zapata et al., 1994)

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly short, monopolar spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

Gene Ontology: Function, Process & Cellular Component ( 8 )

Molecular Function

CV term

Evidence

References

protein binding

inferred from electronic annotation with InterPro:IPR003890, InterPro:IPR016021

(FlyBase Curators et al., 2004-)

translation initiation factor activity

inferred from sequence or structural similarity

(Lasko, 2000)

Biological Process

CV term

Evidence

References

RNA metabolic process

inferred from electronic annotation with InterPro:IPR003890, InterPro:IPR016021

(FlyBase Curators et al., 2004-)

mitotic spindle elongation

inferred from mutant phenotype

(Goshima et al., 2007)

mitotic spindle organization and biogenesis

inferred from mutant phenotype

(Goshima et al., 2007)

translational initiation

inferred from sequence or structural similarity

(Lasko, 2000)

Cellular Component

CV term

Evidence

References

cytosol

inferred from sequence or structural similarity

(Lasko, 2000)

eukaryotic translation initiation factor 4F complex

inferred from sequence or structural similarity

(Lasko, 2000)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

eIF4G allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

68664

Orthologs

Genome-wide drosophilid orthologs

Dana\GF19009

Dere\GG16429

Dgri\GH23978

Dmoj\GI14106

Dper\GL15607

Dpse\GA10575

Dsec\GM26760

Dvir\GJ21224

Dwil\GK13658

Dyak\GE14497

Curated drosophilid orthologs

Dpse\GA10575

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000021865
Anopheles gambiae (Ensembl)
ENSBTAP00000017117
Bos taurus (Ensembl)
ENSCAFP00000022071
Canis familiaris (Ensembl)
ENSCINP00000010674
Ciona intestinalis (Ensembl)
NEWSINFRUP00000137165
Takifugu rubripes (Ensembl)
ENSGALP00000016375
Gallus gallus (Ensembl)
ENSP00000323737
Homo sapiens (Ensembl)
ENSMMUP00000013106
Macaca mulatta (Ensembl)
ENSMODP00000019955
Monodelphis domestica (Ensembl)
ENSPTRP00000026992
Pan troglodytes (Ensembl)
ENSRNOP00000043593
Rattus norvegicus (Ensembl)
GSTENP00004682001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000037631
Xenopus tropicalis (Ensembl)
Q69S49
Oryza sativa (Gramene)
MGI:2384784
Mus musculus (Informatics)
YGL049C
Saccharomyces cerevisiae (Yeastgenome)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

Harvard

PBac{RB}eIF4G^e02087

VDRC

v17002

w¹¹¹⁸; P{GD7098}v17002

v17003

w¹¹¹⁸; P{GD7098}v17003

Genomic Clones ( 3 )

cDNA Clones ( 43 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones