HomeGene Dmel\Nop60B
| General Information | |||
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| Symbol | Dmel\Nop60B | Species | D. melanogaster |
| Name | Nucleolar protein at 60B | Annotation symbol | CG3333 |
| Feature type | protein_coding_gene | FlyBase ID | FBgn0023184 |
| Created / Updated | 2005-01-10/2005-01-10 | ||
| Genomic Location | |||
| Chromosome (arm) | 2R | Recombination map | |
| Cytogenetic map | 60C1-60C2 | Sequence location | 2R:20,062,248..20,073,043 [+] |
| Map ( GBrowse ) | 
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Summary Information
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Automatically generated summary
See sections below for more information | The gene Nucleolar protein at 60B is referred to in FlyBase by the symbol Nop60B (CG3333, FBgn0023184). It has the cytological map location 60C1-60C2. Its sequence location is 2R:20062248..20073043. Its molecular function is described as: pseudouridylate synthase activity; tRNA-pseudouridine synthase activity; RNA binding. It is involved in the biological processes: pseudouridine synthesis; rRNA processing; germ cell development; ribosome biogenesis and assembly; tRNA pseudouridine synthesis. 20 alleles are reported. The phenotypes of these alleles are annotated with: egg chamber; egg; embryo; chaeta; adult thorax; testis; spermatocyte fusome; hub cell; male germline stem cell. It has 2 annotated transcripts and 2 annotated polypeptides. | ||
| External Summaries | |||
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Detailed Mapping Data
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| FlyBase Computed Cytological Location | |||
Cytogenetic map Evidence for location 60C1-60C2
Limits computationally determined from genome sequence between P{EP}EP503 and P{lacW}Nop60Bk05318
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| Experimentally Determined Cytological Location | |||
Cytogenetic map Notes References 60B12-60C1 60C1-60C2 (determined by in situ hybridisation)
60B-60C (determined by in situ hybridisation)
60B13-60C1 (determined by in situ hybridisation)
60C1-60C2 60B12-60B13 60C1--2
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| Experimentally Determined Recombination Data | |||
| Location | |||
| Left of (cM) | |||
| Right of (cM) | |||
| Notes | |||
| Molecular Map Data | |||
Gene Order (in direction of increasing cytology)
References Gene Order (overall orientation not stated) References Overall orientation not stated: Nop60B+ snoRNA:H1+ Nop60B+ | |||
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Gene Model & Products
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Please see the
GBrowse view of
Dmel\Nop60B
for information on other features
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| Comments on Gene Model | |||
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Transcript Data
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| Annotated Transcripts | |||
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) FBtr0100213
2154
508 | |||
| Additional Transcript Data & Comments | |||
| Reported size (kB) | 2.0, 1.8 (northern blot) ~1.5 (northern blot); 1.7 (longest cDNA) | ||
| Comments | |||
| External Data | |||
| Crossreferences | |||
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Polypeptide Data
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| Annotated Polypeptides | |||
Name FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
FBpp0099583
56.8
508
9.88
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| Additional Polypeptide Data & Comments | |||
| Reported size (kD) | 508 (aa); 56 (kD predicted) 508 (aa) | ||
| Comments | Nop60B protein rescues the function of the yeast cbf5 gene. | ||
| External Data | |||
| Linkouts | PANTHER
- Protein classification by function, families, and pathways
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| Crossreferences | InterPro
domains - A database of protein families, domains, and functional sites
• PUA-like (IPR015947)
PUA (IPR002478)
tRNA pseudouridine synthase B, N-terminal (IPR002501)
Putative rRNA pseudouridine synthase (IPR004802)
DKCLD (IPR012960)
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Sequences Consistent with the Gene Model
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| DDBJ
/
EMBL / GenBank | DNA sequence Protein sequence Name | ||
| UniProtKB/Swiss-Prot | |||
| UniProtKB/TrEMBL | |||
| Maps to | |||
| Does NOT map to | |||
| Identified with | |||
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Mapped Features & Mutations
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Please see
GBrowse
or insertion reports for information on insertions of transgenic
constructs and features not listed here
Type Symbol & Location Additional Notes References | |||
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External Data
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| Linkouts | DEDB
- Drosophila exon database: splicing graphs
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| Crossreferences | |||
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Expression Data
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| FlyBase-Curated Data | |||
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Transcript and Protein data | Please see the FlyBase Gene Expression Report for details of gene expression from the literature. | ||
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Summary of Transcript Expression
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Stage Tissue/Position
Reference
embryonic stage | stage 4-14
oogenesis stage,adult stage
nurse cell
embryonic stage
embryonic stage,larval stage
embryonic stage-adult stage
embryonic stage-adult stage
oogenesis stage
ovary
embryonic stage | 0-4 hours
adult stage | female
oogenesis stage,adult stage | stage >=3
oocyte
oogenesis stage
ovary
embryonic stage | 0-6 hours
oogenesis stage,adult stage | stage >=3
nurse cell
embryonic stage | 0-4hr
adult stage | female | |||
| Marker for | |||
| Subcellular Localization | |||
| CV Term | |||
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Summary of Polypeptide Expression
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Stage Tissue/Position
Reference
oogenesis stage,adult stage
germarium
oogenesis stage
oogenesis stage,adult stage
follicle cell
embryonic stage
oogenesis stage
ovary
embryonic stage | |||
| Marker for | |||
| Subcellular Localization | |||
| CV Term | nucleolus | ||
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Microarray Data
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Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) 
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External Data & Images
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| Linkouts | FLIGHT
- Cell culture data for RNAi and other high-throughput technologies
FlyAtlas
- Adult expression by tissue, using Affymetrix Dros2 array
GEO (NCBI)
- Gene expression data: microarray and other high-throughput technologies
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Alleles & Phenotypes
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Summary of Allele Phenotypes
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Lethality Allele Other Phenotypes Allele Sterility Allele sterile (with In(2LR)Px4) sterile (with Nop60BDL10) Phenotype manifest in Allele adult cuticle & adult abdomen adult abdomen & macrochaeta nurse cell & nucleus | |||
