A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Gene Dmel\Prosβ2

General Information
SymbolDmel\Prosβ2SpeciesD. melanogaster
NameProteasome β2 subunitAnnotation symbolCG3329
Feature typeprotein_coding_geneFlyBase IDFBgn0023174
Created / Updated2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)3LRecombination map
Cytogenetic map71B1-71B1Sequence location3L:14,993,056..14,994,421 [+]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene Proteasome β2 subunit is referred to in FlyBase by the symbol Prosβ2 (CG3329, FBgn0023174). It has the cytological map location 71B1. Its sequence location is 3L:14993056..14994421. Its molecular function is described as: endopeptidase activity; threonine endopeptidase activity. It is involved in the biological process ubiquitin-dependent protein catabolic process. 14 alleles are reported. The phenotypes of these alleles are annotated with 14 unique terms, many of which group under: peripheral nervous system; nervous system; anatomical structure; organ system; adult; cytoplasmic part; external sensory organ; intracellular membrane-bounded organelle; tormogen cell; sense organ; imaginal precursor; adult segment. It has one annotated transcript and one annotated polypeptide.

hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
71B1-71B1  
Limits computationally determined from genome sequence between P{PZ}Mpcp00564 and P{PZ}l(3)rO220rO220  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Experimentally Determined Recombination Data
Location
3-42.3
(Holden and Suzuki, 1973)
3-41.5
(Smyth and Belote, 1999)
Left of (cM)
(Holden and Suzuki, 1973)
Right of (cM)
(Smyth and Belote, 1999)
Notes
Mapped relative to the ry+ marker in the P{PZ}btl00208 element.
(Smyth and Belote, 1999)
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
Gene Order (overall orientation not stated)
References
hide Gene Model & Products
Please see the GBrowse view of Dmel\Prosβ2 for information on other features
detailed view FBtr0075672 FBtr0075629 FBtr0075671 FBpp0075425 FBpp0075382 FBpp0075424 FBti0075931 FBti0039492 FBti0067771 FBti0007986 FBti0051000 FBti0052246
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
Prosbeta2-RA
FBtr0075629
 NM_079352
  1031
  272
Additional Transcript Data & Comments
Reported size (kB)
1.0 (northern blot)
(Smyth and Belote, 1999)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
Prosbeta2-PA  
FBpp0075382  
29.8  
272  
8.90  
  NP_524076
  AAF49685
Additional Polypeptide Data & Comments
Reported size (kD)
272 (aa)
(Smyth and Belote, 1999)
Comments
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
•
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
Peptidase T1A, proteasome beta-subunit (IPR000243)
20S proteasome, A and B subunits (IPR001353)
Proteasome, beta-type subunit, conserved site (IPR016050)
MEROPS - An information resource for peptidases and the proteins that inhibit them
•
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AA433215
 
AA433227
 
AA698345
 
AA802889
 
AA803673
 
AA949268
 
AE003532
AAF49685
 
AF025791
AAB82570
 
AF025792
AAB82571
 
AI259931
 
AI260485
 
AI294542
 
AI512374
 
AI515954
 
AI520446
 
AQ025250
 
AW943708
 
AY051976
AAK93400
 
BF488945
 
BF492075
 
BF503938
 
BI166541
 
BI215396
 
BI229472
 
BI357895
 
BI362543
 
BI364010
 
BI366988
 
BI638484
 
BP559263
 
CK657983
 
CK658703
 
CK660915
 
CO183668
 
CO191699
 
CO193059
 
CO281136
 
CO293301
 
CO314229
 
CO322091
 
CZ485631
 
CZ487172
 
CZ487820
 
EC075765
 
EC084626
 
EC088245
 
EC222057
 
EC223421
 
EC252090
 
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
    Maps to
    Does NOT map to
    Identified with
    (BDGP Project Members, 2000-)
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    point mutation
    Prosbeta2[1]
    3L:14,994,007..14,994,007
    comment=Difference between reported amino acid substitution and FlyBase curator due to differences in strain sequence. Authors sequence has G170 encoded by GGA while FlyBase sequence is GGT.
    evidence=experimental
    na_change=G14994007A
    pr_change=G169S|Prosbeta2-PA
    reported_na_change=G945A
    reported_pr_change=G170R
    (Smyth and Belote, 1999)
    rescue fragment
    Prosβ2[+t2.7]
    3L:14,992,102..14,994,879
    comment=Position of restriction fragment on reference sequence inferred by FlyBase curator.
    evidence=experimental
    linked_to=PstI-EcoRI_rfrag
    (Labourier et al., 2002)
    hide External Data
    Linkouts
    DEDB - Drosophila exon database: splicing graphs
    •
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
     embryonic stage-adult stage
     
    (Smyth and Belote, 1999)
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
      Marker for
        Subcellular Localization
        CV Term
        hide Microarray Data
        Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)
        Untitled Document detailed view FBtr0075672 FBtr0075629 FBtr0075671
        AI512374
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Lethality
        Allele
        lethal | dominant | conditional - heat sensitive
        lethal | dominant | pupal stage | conditional - heat sensitive
        lethal | larval stage | recessive
        Other Phenotypes
        Allele
        wild-type
        visible | dominant | conditional - heat sensitive
        visible | conditional - heat sensitive, with Scer\GAL4GMR.PF
        visible, with Scer\GAL4GMR.PF
        Phenotype manifest in
        Allele
        macrochaeta
        microchaeta
        tormogen cell | ectopic
        thecogen cell | ectopic
        trichogen cell
        eo neuron
        imaginal disc | conditional - heat sensitive
        adult abdomen | conditional - heat sensitive
        wing vein | ectopic | conditional - heat sensitive
        eye, with Scer\GAL4GMR.PF
        ommatidium, with Scer\GAL4GMR.PF
        eye | conditional - heat sensitive, with Scer\GAL4GMR.PF
        multivesicular body | supernumerary | conditional - heat sensitive, with Scer\GAL4GMR.PF
        autophagic vacuole | supernumerary | conditional - heat sensitive, with Scer\GAL4GMR.PF
        eye photoreceptor cell | conditional - heat sensitive, with Scer\GAL4GMR.PF
        hide Classical Alleles ( 10 )
        For All Classical Alleles Show

        Allele of Prosβ2ClassMutagenStocksKnown lesion
        Prosβ210 Yes
        Prosβ2EP30672 --
        Prosβ2f030701 --
        Prosβ2f046321 --
        Prosβ2f051951 --
        Prosβ2rv11γ0 --
        Prosβ2rv12γ0 --
        Prosβ2rv19X0 --
        Prosβ2rv3D0 --
        Prosβ2rv3γ0 --
        hide Alleles Carried on Transgenic Constructs ( 4 )
        For All Alleles Carried on Transgenic Constructs Show

        Allele of Prosβ2ClassMutagenStocksKnown lesion
        Prosβ2+t2.70 Yes
        Prosβ21.Scer\UAS2 Yes
        Prosβ2GD109381 Yes
        Prosβ2GD164380 Yes
        hide Aneuploid Aberrations
        Useful deficiency
         
         
        Useful duplication
         
        Disrupted in
        (Smyth and Belote, 1999)
        (Smyth and Belote, 1999)
        (Smyth and Belote, 1999)
        (Ryder, 2004.4.26)
        (Ryder, 2004.4.26)
        (Smyth and Belote, 1999)
        Not disrupted in
        (Smyth and Belote, 1999)
        (Smyth and Belote, 1999)
        (Smyth and Belote, 1999)
        (Smyth and Belote, 1999)
        hide Transgenic Constructs & Insertions
        Transgenic Constructs
        Type of construct
        Name
        Expression data
        UAS construct
        P{UAS-Prosβ21}
        NA
        characterization construct
        P{Prosβ2+t2.7}
        NA
        Insertions
        Type of insertions
        Name
        Expression data
        miscellaneous insertions
        PBac{WH}Prosβ2f03070
        NA
        PBac{WH}Prosβ2f04632
        NA
        PBac{WH}Prosβ2f05195
        NA
        insertion of mobile activating element
        P{EP}Prosβ2EP3067
        NA
        hide Related Comments
        Please look at the allele reports for the complete phenotype data
        Mutations affecting two distinct proteosome catalytic subunits, Pros26 and Prosβ2 affect two alternative cell fate decisions and enhance N signalling activity in the sense organ lineage.
        (Schweisguth, 1999)
        RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
        (Boutros et al., 2004)
        hide Gene Ontology: Function, Process & Cellular Component ( 4 )
        hide Molecular Function
        CV term
        Evidence
        References
        endopeptidase activity
        inferred from sequence or structural similarity with UniProtKB:O35955
        (FlyBase, 1992-)
        threonine endopeptidase activity
        inferred from electronic annotation with InterPro:IPR001353
        (FlyBase Curators et al., 2004-)
        hide Biological Process
        CV term
        Evidence
        References
        ubiquitin-dependent protein catabolic process
        inferred from electronic annotation with InterPro:IPR000243, InterPro:IPR001353
        (FlyBase Curators et al., 2004-)
        hide Cellular Component
        CV term
        Evidence
        References
        proteasome core complex
        inferred from sequence or structural similarity with UniProtKB:O35955
        (FlyBase, 1992-)
        hide Sequence Ontology: Class of Gene
        protein_coding_gene
         
        nuclear_gene
         
        hide Interactions & Pathways
        hide Summary of Genetic Interactions
        Interacts with
        Please look at the allele data for full details of the genetic interactions
        Prosβ2 allele
        Gene
        References
        Prosβ21.Scer\UAS
        Pros26
        (Belote and Fortier, 2002)
        Prosβ21
        N
        (Schweisguth, 1999)
        Pros26
        (Smyth and Belote, 1999, Schweisguth, 1999)
        H
        (Schweisguth, 1999)
        vn
        (Donaldson et al., 2004)
        unspecified
        HDAC6
        (Pandey et al., 2007)
        Pros26
        (Neuburger et al., 2006)
        Atg6
        (Pandey et al., 2007)
        Atg12
        (Pandey et al., 2007)
        Pros25
        (Neuburger et al., 2006, Smyth and Belote, 1999)
        hide External Data
        Linkouts
        BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
        •
        hide Orthologs
        Genome-wide drosophilid orthologs
        Curated drosophilid orthologs
         
        Linkouts
        InParanoid orthologs - Eukaryotic orthologs
        •
        hide Functional Complementation between Species
        hide Inter-Species Misexpression Data
        Produces phenotype in
        Produces NO phenotype in
        hide Stocks & Reagents
        hide Stocks Listed in FlyBase ( 8 )
        Szeged
        -
        P{EP}Prosβ2EP3067
        Harvard
        -
        PBac{WH}Prosβ2f03070
        -
        PBac{WH}Prosβ2f04632
        -
        PBac{WH}Prosβ2f05195
        VDRC
        v24749
        w1118; P{GD10938}v24749
        Bloomington
        6785
        w1118; P{UAS-Prosβ21}1B
        17084
        w1118; P{EP}Prosβ2EP3067/TM6B, Tb1
        6787
        w1118; P{UAS-Pros261.B}2B; P{UAS-Prosβ21}1B
        hide Genomic Clones ( 2 )
        hide cDNA Clones ( 33 )
        Please Note
        This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
        cDNA Clones, Fully Sequenced
        BDGP DGC clones
        Other clones
        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        hide RNAi & Array Information
        Affy Oligo
        Linkouts
        DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
        •
        GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
        •
        hide Antibody Information
        hide Other Information
        hide Discoverer
        hide Etymology
        hide Identification
        hide Position Effect Variegation Data
        hide Relationship to Other Genes
        Source for database identity of
        Source for database merge of
        Source for merge of: Prosβ2 anon-EST:fe1E6 anon-EST:fe1G6
        (Bayraktaroglu, 2000.11.2)
        Additional comments
        hide Comments About Role
        A wild type level of proteosome activity is required for the proper regulation of at least two alternative cell fate decisions during sense organ development. Proteosome-mediated protein degradation antagonizes N signalling activity, perhaps by targeting an active form of N protein for degradation.
        (Schweisguth, 1999)
        hide Comments About Molecular Function
        hide Other Comments
        hide External Crossreferences & Linkouts
        Sequence Crossreferences
        RefSeq (Transcripts)
        •
        RefSeq (Proteins)
        •
        DDBJ / EMBL / GenBank
        •