Gene Dmel\CycH

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General Information

Symbol

Dmel\CycH

Species

D. melanogaster

Name

Cyclin H

Annotation symbol

CG7405

Feature type

protein_coding_gene

FlyBase ID

FBgn0022936

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

78F3-78F3

Sequence location

3L:21,815,101..21,816,509 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Cyclin H is referred to in FlyBase by the symbol CycH (CG7405, FBgn0022936). It has the cytological map location 78F3. Its sequence location is 3L:21815101..21816509. Its molecular function is described as: general RNA polymerase II transcription factor activity; cyclin-dependent protein kinase regulator activity. It is involved in the biological processes: transcription initiation from RNA polymerase II promoter; cell cycle. 4 alleles are reported. The phenotype of these alleles is annotated with spermatogonium. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

78F3-78F3

Limits computationally determined from genome sequence between P{lacW}l(3)j1B10^j1B10 and P{lacW}l(3)j2C4^j2C4

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\CycH for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

CycH-RA

FBtr0078439

NM_079483

1161

324

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

CycH-PA

FBpp0078093

37.8

324

5.86

NP_524207

AAF51784

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0022936

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Cyclin ccl1 (IPR005258)

Cyclin (IPR006670)

Cyclin, N-terminal (IPR006671)

Cyclin-like (IPR011028)

Cyclin-related (IPR013763)

Transcription regulator cyclin (IPR015429)

Cyclin H (IPR015432)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

SD26182

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

6058

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0022936

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0022936

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0022936

Alleles & Phenotypes

Summary of Allele Phenotypes

Other Phenotypes

Allele

cell death increase, with Scer\GAL4^ptc-559.1

CycH^EP3658

Phenotype manifest in

Allele

spermatogonium, with Scer\GAL4^ptc-559.1

CycH^EP3658

Classical Alleles ( 3 )

For All Classical Alleles Show

Allele of CycH	Mutagen	Stocks	Known lesion
CycH^EP3658		1	--
CycH^GS9043		0	Yes
CycH^KG02273	P-element activity	1	--

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of CycH	Class	Mutagen	Stocks	Known lesion
CycH^GD4137		in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes

Aneuploid Aberrations

Useful deficiency

Df(3L)78E2-3;79E

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD4137}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{SUPor-P}CycH^KG02273

insertion of enhancer trap

P{lacW}l(3)SH160^SH160

insertion of mobile activating element

P{EP}CycH^EP3658

P{GSV6}CycH^GS9043

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 5 )

Molecular Function

CV term

Evidence

References

cyclin-dependent protein kinase regulator activity

inferred from sequence or structural similarity with UniProtKB:P51947

(FlyBase, 1992-)

general RNA polymerase II transcription factor activity

inferred from sequence or structural similarity with HGNC_gene:CCNH; HGNC:1594; OMIM:601953 AND inferred from sequence or structural similarity with SGD_LOCUS:CCL1; SGD:S0006229

(Aoyagi and Wassarman, 2000)

Biological Process

CV term

Evidence

References

cell cycle

inferred from electronic annotation with InterPro:IPR005258

(FlyBase Curators et al., 2004-)

transcription initiation from RNA polymerase II promoter

inferred from sequence or structural similarity with HGNC_gene:CCNH; HGNC:1594; OMIM:601953 AND inferred from sequence or structural similarity with SGD_LOCUS:CCL1; SGD:S0006229

(Aoyagi and Wassarman, 2000)

Cellular Component

CV term

Evidence

References

holo TFIIH complex

inferred from sequence or structural similarity with HGNC_gene:CCNH; HGNC:1594; OMIM:601953 AND inferred from sequence or structural similarity with SGD_LOCUS:CCL1; SGD:S0006229

(Aoyagi and Wassarman, 2000)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

CycH allele

Gene

References

unspecified

pbl

(Gregory et al., 2007)

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

65669

Drosophila PIMRider - The Drosophila Protein Interaction map

•

522725

Orthologs

Genome-wide drosophilid orthologs

Dana\GF25171

Dere\GG16226

Dgri\GH16374

Dmoj\GI11567

Dper\GL24864

Dpse\GA20328

Dsec\GM22410

Dsim\GD15002

Dvir\GJ11246

Dwil\GK16840

Dyak\GE19794

Curated drosophilid orthologs

Dpse\GA20328

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000019240
Anopheles gambiae (Ensembl)
ENSBTAP00000015470
Bos taurus (Ensembl)
ENSCAFP00000012312
Canis familiaris (Ensembl)
NEWSINFRUP00000142883
Takifugu rubripes (Ensembl)
ENSGALP00000025170
Gallus gallus (Ensembl)
ENSP00000256897
Homo sapiens (Ensembl)
ENSMMUP00000004329
Macaca mulatta (Ensembl)
ENSMODP00000025305
Monodelphis domestica (Ensembl)
ENSPTRP00000029206
Pan troglodytes (Ensembl)
ENSRNOP00000039039
Rattus norvegicus (Ensembl)
GSTENP00028721001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000025634
Xenopus tropicalis (Ensembl)
Q5GA21
Oryza sativa (Gramene)
AT5G27620.1
Arabidopsis thaliana (Arabidopsis)
WBGene00037307
Caenorhabditis briggsae (Wormbase)
WBGene00021714
Caenorhabditis elegans (Wormbase)
ZDB-GENE-050320-13
Danio rerio (Zfin)
DDB0231776
Dictyostelium discoideum (Dictybase)
MGI:1913921
Mus musculus (Informatics)
YPR025C
Saccharomyces cerevisiae (Yeastgenome)
SPBP16F5.02
Schizosaccharomyces pombe (Sanger)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

Szeged

P{EP}CycH^EP3658

VDRC

v10398

w¹¹¹⁸; P{GD4137}v10398

Bloomington

13200

y¹ w^67c23; P{SUPor-P}CycH^KG02273 ry⁵⁰⁶

Genomic Clones ( 1 )

BACR15K24

cDNA Clones ( 4 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

SD26182

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0022936

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0022936

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for identity of: CycH CG7405

(Li, 1999.11)

Source for database merge of

Additional comments

Comments About Role

Comments About Molecular Function

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_079483

RefSeq (Proteins)

•

NP_524207

DDBJ / EMBL / GenBank

•

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Cyclin ccl1 (IPR005258)

Cyclin (IPR006670)

Cyclin, N-terminal (IPR006671)

Cyclin-like (IPR011028)

Cyclin-related (IPR013763)

Transcription regulator cyclin (IPR015429)

Cyclin H (IPR015432)

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

65669

DEDB - Drosophila exon database: splicing graphs

•

6058

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0022936

Drosophila PIMRider - The Drosophila Protein Interaction map

•

522725

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0022936

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0022936

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0022936

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0022936

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0022936

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000019240
Anopheles gambiae (Ensembl)
ENSBTAP00000015470
Bos taurus (Ensembl)
ENSCAFP00000012312
Canis familiaris (Ensembl)
NEWSINFRUP00000142883
Takifugu rubripes (Ensembl)
ENSGALP00000025170
Gallus gallus (Ensembl)
ENSP00000256897
Homo sapiens (Ensembl)
ENSMMUP00000004329
Macaca mulatta (Ensembl)
ENSMODP00000025305
Monodelphis domestica (Ensembl)
ENSPTRP00000029206
Pan troglodytes (Ensembl)
ENSRNOP00000039039
Rattus norvegicus (Ensembl)
GSTENP00028721001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000025634
Xenopus tropicalis (Ensembl)
Q5GA21
Oryza sativa (Gramene)
AT5G27620.1
Arabidopsis thaliana (Arabidopsis)
WBGene00037307
Caenorhabditis briggsae (Wormbase)
WBGene00021714
Caenorhabditis elegans (Wormbase)
ZDB-GENE-050320-13
Danio rerio (Zfin)
DDB0231776
Dictyostelium discoideum (Dictybase)
MGI:1913921
Mus musculus (Informatics)
YPR025C
Saccharomyces cerevisiae (Yeastgenome)
SPBP16F5.02
Schizosaccharomyces pombe (Sanger)

PANTHER - Protein classification by function, families, and pathways

•

FBgn0022936

Synonyms & Secondary IDs ( 6 )

Reported As

Symbol Synonym

142767_at

(Kristensen et al., 2005)

CG7405

(Aoyagi and Wassarman, 2000, )

CycH

Name Synonym

cyclin H

(Brumby et al., 2004, Larochelle et al., 2001, Leclerc et al., 2000, Peng et al., 1998)

Cyclin H

(Gregory et al., 2007)

CyclinH

(Gregory et al., 2007)

Secondary FlyBase IDs

References ( 18 )

Research paper

Gregory et al., 2007, Fly 1(1): 13--22: A Drosophila Overexpression Screen for Modifiers of Rho Signalling in Cytokinesis. [FBrf0199094]
Kristensen et al., 2005, Genetics 171(1): 157--167: Genome-wide analysis on inbreeding effects on gene expression in Drosophila melanogaster. [FBrf0187643]
Brumby et al., 2004, Genetics 168(1): 227--251: A genetic screen for dominant modifiers of a cyclin E hypomorphic mutation identifies novel regulators of S-phase entry in Drosophila. [FBrf0180291]
Schulz et al., 2004, Genetics 167(2): 707--723: A misexpression screen reveals effects of bag-of-marbles and TGF beta class signaling on the Drosophila male germ-line stem cell lineage. [FBrf0179436]
Larochelle et al., 2001, EMBO J. 20(14): 3749--3759: T-loop phosphorylation stabilizes the CDK7-cyclin H-MAT1 complex in vivo and regulates its CTD kinase activity. [FBrf0137227]
Aoyagi and Wassarman, 2000, J. Cell Biol. 150(2): F45--F49: Genes encoding Drosophila melanogaster RNA polymerase II general transcription factors: diversity in TFIIA and TFIID components contributes to gene-specific transcriptional regulation. [FBrf0129705]
Leclerc et al., 2000, EMBO J. 19(7): 1567--1575: Dominant-negative mutants reveal a role for the cdk7 kinase at the mid-blastula transition in Drosophila embryos. [FBrf0127187]
Peng et al., 1998, J. Biol. Chem. 273(22): 13855--13860: Identification of a cyclin subunit required for the function of Drosophila P-TEFb. [FBrf0103005]

Supplementary material

Giot et al., 2003, Science 302(5651):: Tables S1 to S7. [FBrf0166452]
Kiger et al., 2003, J. Biol. 2(4):: Additional data file 1: The gene identity and primer sequences for dsRNAs used in the RNAi screens. [FBrf0174231]
Oh et al., 2003, Genetics 163(1):: Table S1. [FBrf0157097]

Personal communication to FlyBase

Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms.: Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]

FlyBase analysis

FlyBase, 1992-, FlyBase curation: FlyBase curation. [FBrf0105495]

Curated genome annotation

Li, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126682]

Computer file

Gene Disruption Project members, 2001-, [title not yet available]: [title not yet available] [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project.: Berkeley Drosophila Genome Project. [FBrf0125078]

Automatic genome annotation

FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126705]