Gene Dmel\Hel89B

(Goldman-Levi et al., 1995)

89B-89B

(determined by in situ hybridisation)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Maps immediately to the left of Sb.

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

In direction of increasing cytology: mor- Hel89B+

(van der Knaap et al., 1998)

Gene Order (overall orientation not stated)

References

Overall orientation not stated: mor? Hel89B?

Gene Model & Products

Please see the GBrowse view of Dmel\Hel89B for information on other features

Comments on Gene Model

DGC clone GH12153 appears problematic: incomplete CDS

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Hel89B-RA

FBtr0083214

NM_169693

5930

1929

Additional Transcript Data & Comments

Reported size (kB)

7.4 (northern blot)

7.5 (northern blot)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Hel89B-PA

FBpp0082668

213.8

1929

7.00

NP_732097

AAF55260

Additional Polypeptide Data & Comments

Reported size (kD)

210 (kD observed)

Comments

External Data

(BDGP Project Members, 2000-)

PANTHER - Protein classification by function, families, and pathways

•

FBgn0022787

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

SNF2-related (IPR000330)

HEAT (IPR000357)

DNA/RNA helicase, C-terminal (IPR001650)

Armadillo-like helical (IPR011989)

DEAD-like helicase, N-terminal (IPR014001)

Helicase, superfamily 1 and 2, ATP-binding (IPR014021)

Armadillo-type fold (IPR016024)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

DEDB - Drosophila exon database: splicing graphs

•

9626

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

embryonic stage-adult stage

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

unfertilized egg

embryonic stage | stage >=12

longitudinal connectives

embryonic stage | early

embryonic stage | stage >=12

embryonic brain

embryonic stage | stage >=12

ventral nerve cord

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI134634
AI134258

External Data & Images

Show all BDGP original images, annotations and embryonic microarray data for Hel89B

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0022787

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0022787

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0022787

BDGP expression data

•

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for Hel89B

Alleles & Phenotypes

Summary of Allele Phenotypes

Other Phenotypes

Allele

visible | recessive

Phenotype manifest in

Allele

abdominal sternite 6 | male

Classical Alleles ( 5 )

For All Classical Alleles Show

Allele of Hel89B	Mutagen	Stocks	Known lesion
Hel89B¹⁰	P-element activity	0	Yes
Hel89B^14-77		0	--
Hel89B^89B	Δ2-3	0	--
Hel89B⁹	P-element activity	0	Yes
Hel89B^HP31401		1	--

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Hel89B	Class	Mutagen	Stocks	Known lesion
Hel89B^GD4237		in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
Hel89B^t11.8		in vitro construct \| genomic fragment	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(3R)sbd105

Useful duplication

Dp(3;3)C123.3

Disrupted in

Df(3R)Exel7327

(Ryder, 2004.4.26)

Df(3R)mor¹⁰

Df(3R)mor⁹

P{Ecol\lacO.256x.w}P25.22

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD4237}

characterization construct

P{mor⁺11.8}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

insertion of enhancer trap

P{lacW}Hel89B^89B

P{lacW}l(3)SH032^SH032

insertion of mobile activating element

P{EPg}Hel89B^HP31401

P{GSV1}EP-767.1^EP-767.1

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 8 )

Molecular Function

CV term

Evidence

References

ATP binding

inferred from electronic annotation with InterPro:IPR000330, InterPro:IPR001650

(FlyBase Curators et al., 2004-)

ATP-dependent DNA helicase activity

inferred from sequence or structural similarity with FLYBASE:brm; FB:FBgn0000212 AND inferred from sequence or structural similarity with SGD_LOCUS:MOT1; SGD:S0006003

(FlyBase Curators et al., 2004-)

DNA binding

inferred from electronic annotation with InterPro:IPR000330

helicase activity

non-traceable author statement

(Goldman-Levi, 1996.1.11)

Biological Process

CV term

Evidence

References

Toll signaling pathway

inferred from mutant phenotype

antimicrobial humoral response

inferred from mutant phenotype

(Kleino et al., 2005)

innate immune response

inferred from mutant phenotype

positive regulation of antibacterial peptide biosynthetic process

inferred from mutant phenotype

Cellular Component

CV term

Evidence

References

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Hel89B allele

Gene

References

Hel89B^89B

Ubx

External Data

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

66997

Orthologs

Genome-wide drosophilid orthologs

Dana\GF18554

Dere\GG16968

Dgri\GH23502

Dmoj\GI22957

Dper\GL22086

Dpse\GA18064

Dsec\GM24274

Dvir\GJ23773

Dwil\GK12181

Dyak\GE24355

Curated drosophilid orthologs

Dpse\GA18064

Dsim\Hel89B

(Swanson et al., 2001)

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000008413
Anopheles gambiae (Ensembl)
ENSBTAP00000021438
Bos taurus (Ensembl)
ENSCAFP00000010955
Canis familiaris (Ensembl)
ENSCINP00000002650
Ciona intestinalis (Ensembl)
NEWSINFRUP00000143763
Takifugu rubripes (Ensembl)
ENSGALP00000011221
Gallus gallus (Ensembl)
ENSP00000265990
Homo sapiens (Ensembl)
ENSMMUP00000023195
Macaca mulatta (Ensembl)
ENSMODP00000008303
Monodelphis domestica (Ensembl)
ENSPTRP00000004803
Pan troglodytes (Ensembl)
ENSRNOP00000024465
Rattus norvegicus (Ensembl)
GSTENP00006554001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000026599
Xenopus tropicalis (Ensembl)
Q6H7U1
Oryza sativa (Gramene)
AT3G54280.1
Arabidopsis thaliana (Arabidopsis)
WBGene00025797
Caenorhabditis briggsae (Wormbase)
WBGene00000274
Caenorhabditis elegans (Wormbase)
DDB0186863
Dictyostelium discoideum (Dictybase)
MGI:2147538
Mus musculus (Informatics)
YPL082C
Saccharomyces cerevisiae (Yeastgenome)
SPBC1826.01c
Schizosaccharomyces pombe (Sanger)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

Szeged

P{lacW}l(3)SH032^SH032

VDRC

v30495

w¹¹¹⁸; P{GD4237}v30495/TM3

Bloomington

21961

w¹¹¹⁸; P{EPg}Hel89B^HP31401

Genomic Clones ( 2 )

cDNA Clones ( 18 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

SD16865

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0022787

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0022787

Antibody Information

polyclonal

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Comments About Role

Cloning and characterisation of Hel89B suggests the protein may be a global transcriptional regulator of many target genes that functions throughout development.

Show all BDGP original images, annotations and embryonic microarray data for Hel89B

Comments About Molecular Function

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a greater than three-fold increase in AttA activity in response to heat-killed E.coli after ecdysone treatment in S2 cells.

(Kleino et al., 2005)

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_169693

RefSeq (Proteins)

•

NP_732097

DDBJ / EMBL / GenBank

•

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

SNF2-related (IPR000330)

HEAT (IPR000357)

DNA/RNA helicase, C-terminal (IPR001650)

Armadillo-like helical (IPR011989)

DEAD-like helicase, N-terminal (IPR014001)

Helicase, superfamily 1 and 2, ATP-binding (IPR014021)

Armadillo-type fold (IPR016024)

Linkouts

BDGP expression data

•

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

66997

DEDB - Drosophila exon database: splicing graphs

•

9626

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0022787

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0022787

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0022787

FlyExpress - Embryonic expression images (BDGP data)

•

FBgn0022787

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0022787

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0022787

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0022787

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000008413
Anopheles gambiae (Ensembl)
ENSBTAP00000021438
Bos taurus (Ensembl)
ENSCAFP00000010955
Canis familiaris (Ensembl)
ENSCINP00000002650
Ciona intestinalis (Ensembl)
NEWSINFRUP00000143763
Takifugu rubripes (Ensembl)
ENSGALP00000011221
Gallus gallus (Ensembl)
ENSP00000265990
Homo sapiens (Ensembl)
ENSMMUP00000023195
Macaca mulatta (Ensembl)
ENSMODP00000008303
Monodelphis domestica (Ensembl)
ENSPTRP00000004803
Pan troglodytes (Ensembl)
ENSRNOP00000024465
Rattus norvegicus (Ensembl)
GSTENP00006554001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000026599
Xenopus tropicalis (Ensembl)
Q6H7U1
Oryza sativa (Gramene)
AT3G54280.1
Arabidopsis thaliana (Arabidopsis)
WBGene00025797
Caenorhabditis briggsae (Wormbase)
WBGene00000274
Caenorhabditis elegans (Wormbase)
DDB0186863
Dictyostelium discoideum (Dictybase)
MGI:2147538
Mus musculus (Informatics)
YPL082C
Saccharomyces cerevisiae (Yeastgenome)
SPBC1826.01c
Schizosaccharomyces pombe (Sanger)

PANTHER - Protein classification by function, families, and pathways

•

FBgn0022787

University of Chicago Dev. Expression

•

FBgn0022787

Synonyms & Secondary IDs ( 8 )

Reported As

Symbol Synonym

89B

(Adamkewicz et al., 2001)

BTAF1

(Tora, 2002, Yu et al., 2002)

CG4261

(Molnar et al., 2006)

dTAF_II230

(Pereira et al., 2001)

Hel89B

(Molnar et al., 2006)

Helicase89B