A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Gene Dmel\Orc1

General Information
SymbolDmel\Orc1SpeciesD. melanogaster
NameOrigin recognition complex subunit 1Annotation symbolCG10667
Feature typeprotein_coding_geneFlyBase IDFBgn0022772
Created / Updated2003-12-02/2003-12-02
Genomic Location
Chromosome (arm)2RRecombination map
Cytogenetic map43D1-43D2Sequence location2R:3,409,940..3,413,213 [-]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene Origin recognition complex subunit 1 is referred to in FlyBase by the symbol Orc1 (CG10667, FBgn0022772). It has the cytological map location 43D1-43D2. Its sequence location is 2R:3409940..3413213. Its molecular function is described as: DNA binding; ATP binding; nucleoside-triphosphatase activity. It is involved in the biological processes: DNA-dependent DNA replication; DNA replication initiation; chromatin silencing. 15 alleles are reported. The phenotypes of these alleles are annotated with: eye; ommatidium; interommatidial bristle; eye-antennal disc; morphogenetic furrow; stage S10B oocyte; egg; chorion; dorsal appendage. It has one annotated transcript and one annotated polypeptide.
External Summaries

hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
43D1-43D2  
Limits computationally determined from genome sequence between P{lacW}cosk16101 and P{EP}CG1600EP398  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
43E1-43E2
(determined by in situ hybridisation)  
(Chesnokov et al., 1999)
43D1-43D7
(determined by in situ hybridisation)  
(BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
Gene Order (overall orientation not stated)
References
hide Gene Model & Products
Please see the GBrowse view of Dmel\Orc1 for information on other features
detailed view FBtr0088904 FBtr0088959 FBtr0088958 FBtr0088957 FBtr0088956 FBpp0087978 FBpp0088033 FBpp0088030 FBpp0088032 FBpp0088031
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
Orc1-RA
FBtr0088959
 NM_057955
  3146
  924
Additional Transcript Data & Comments
Reported size (kB)
2.7 (northern blot)
(Chesnokov et al., 1999)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
Orc1-PA  
FBpp0088033  
103.3  
924  
9.84  
  NP_477303
  AAF59236
Additional Polypeptide Data & Comments
Reported size (kD)
924 (aa)
(Pak et al., 1997)
Comments
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
•
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
Bromo adjacent region (IPR001025)
AAA+ ATPase, core (IPR003593)
AAA ATPase, core (IPR003959)
CDC6, C terminal (IPR015163)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AA246286
 
AA391691
 
AA539062
 
AC005641
 
AE003840
AAF59236
 
AF017647
AAC47802
 
AI512478
 
AW942195
 
AY094780
AAM11133
 
BI577609
 
BQ103001
 
CG10667
 
CO187046
 
CO262357
 
CO262749
 
CO290990
 
CO291387
 
CO293356
 
CO301511
 
CO302305
 
CO339733
 
CO341502
 
EC052075
 
EC059146
 
EC073780
 
EC078279
 
EC200077
 
EC201255
 
EC206420
 
EC207968
 
EC215630
 
EC230903
 
EC230995
 
EC231098
 
EC233374
 
EC246147
 
EC251264
 
EC255248
 
EC258286
 
Y18882
 
 
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
    Maps to
    (BDGP Project Members, 1994-1999, FlyBase, 1992-)
    Does NOT map to
    Identified with
    (BDGP Project Members, 2000-, Badugu et al., 2003)
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    protein binding site
    Orc1-protein_bind-1
    2R:3,413,214..3,413,221
    bound_moiety=E2f-XP
    comment=One of two overlapping candidate E2f protein binding sites, which, when mutated, lose the ability to compete for E2f binding
    evidence=experimental
    (Asano and Wharton, 1999)
    protein binding site
    Orc1-protein_bind-2
    2R:3,413,211..3,413,218
    bound_moiety=E2f-XP
    comment=One of two overlapping candidate E2f protein binding sites, which, when mutated, lose the ability to compete for E2f binding
    evidence=experimental
    (Asano and Wharton, 1999)
    hide External Data
    Linkouts
    DEDB - Drosophila exon database: splicing graphs
    •
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
     embryonic stage,larval stage
     
    (Chesnokov et al., 1999)
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
      Marker for
        Subcellular Localization
        CV Term
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Lethality
        Allele
        lethal | polyphasic
        Other Phenotypes
        Allele
        visible
        Sterility
        Allele
        female sterile
        male sterile
        Phenotype manifest in
        Allele
        eye
        ommatidium
        interommatidial bristle
        eye-antennal disc | posterior
        morphogenetic furrow
        stage S10B oocyte
        follicle cell & nucleus
        egg
        chorion
        dorsal appendage
        hide Classical Alleles ( 0 )
        For All Classical Alleles Show

        Allele of Orc1ClassMutagenStocksKnown lesion
        hide Alleles Carried on Transgenic Constructs ( 15 )
        For All Alleles Carried on Transgenic Constructs Show

        Allele of Orc1ClassMutagenStocksKnown lesion
        Orc11-248.Scer\UAS.T:Avic\GFP0 Yes
        Orc1242-556.3xmdb.Scer\UAS.T:Avic\GFP0 Yes
        Orc1242-556.Scer\UAS.T:Avic\GFP0 Yes
        Orc1C.Scer\UAS.T:Avic\GFP,T:Zzzz\nls10 Yes
        Orc1C.Scer\UAS.T:Avic\GFP0 Yes
        Orc1GD44772 Yes
        Orc1K604E.hs0 Yes
        Orc1MtnA.T:Avic\GFP0 Yes
        Orc1N.Scer\UAS.T:Avic\GFP0 Yes
        Orc1N.mkb.Scer\UAS.T:Avic\GFP0 Yes
        Orc1Scer\UAS.T:Avic\GFP0 Yes
        Orc1T:Avic\GFP,T:Hsap\MYC0 Yes
        Orc1hs.PA0 Yes
        Orc1mkb.3xmdb.Scer\UAS.T:Avic\GFP0 Yes
        Orc1mob.Scer\UAS.T:Avic\GFP0 Yes
        hide Aneuploid Aberrations
        Useful deficiency
         
        Disrupted in
        (Ryder, 2004.4.26)
        hide Transgenic Constructs & Insertions
        Transgenic Constructs
        Type of construct
        Name
        Expression data
        UAS construct
        P{GD4477}
        NA
        P{UAS-Orc1.1-248.GFP}
        NA
        P{UAS-Orc1.242-556.3xmdb.GFP}
        NA
        P{UAS-Orc1.242-556.GFP}
        NA
        P{UAS-Orc1.C.GFP.nls}
        NA
        P{UAS-Orc1.C.GFP}
        NA
        P{UAS-Orc1.GFP}
        NA
        P{UAS-Orc1.N.GFP}
        NA
        P{UAS-Orc1.N.mkb.GFP}
        NA
        P{UAS-Orc1.mkb.3xmdb.GFP}
        NA
        P{UAS-Orc1.mob.GFP}
        NA
        characterization construct
        P{Orc1-GFP.A}
        NA
        heat-shock construct
        P{hs-Orc1.A}
        NA
        P{hs-Orc1.K604E}
        NA
        Insertions
        Type of insertions
        Name
        Expression data
        hide Related Comments
        Please look at the allele reports for the complete phenotype data
        hide Gene Ontology: Function, Process & Cellular Component ( 8 )
        hide Molecular Function
        CV term
        Evidence
        References
        ATP binding
        inferred from electronic annotation with InterPro:IPR003959
        (FlyBase Curators et al., 2004-)
        DNA binding
        non-traceable author statement
        (Swiss-Prot Project Members, 1999.4.15)
        inferred from electronic annotation with InterPro:IPR001025
        (FlyBase Curators et al., 2004-)
        nucleoside-triphosphatase activity
        inferred from electronic annotation with InterPro:IPR003593
        (FlyBase Curators et al., 2004-)
        hide Biological Process
        CV term
        Evidence
        References
        DNA replication initiation
        inferred from direct assay
        (Chesnokov et al., 1999)
        DNA-dependent DNA replication
        inferred from sequence or structural similarity with EMBL:AJ003133; protein_id:CAA05890
        (FlyBase, 1992-)
        non-traceable author statement
        (Chesnokov, 1997.8.6)
        non-traceable author statement
        (Swiss-Prot Project Members, 1999.4.15)
        chromatin silencing
        non-traceable author statement
        (Swiss-Prot Project Members, 1999.4.15)
        hide Cellular Component
        CV term
        Evidence
        References
        nuclear origin of replication recognition complex
        inferred from sequence or structural similarity with EMBL:AJ003133; protein_id:CAA05890
        (FlyBase, 1992-)
        inferred from direct assay
        (Chesnokov et al., 1999)
        non-traceable author statement
        (Chesnokov, 1997.8.6)
        non-traceable author statement
        (Swiss-Prot Project Members, 1999.4.15)
        nucleus
        non-traceable author statement
        (Chesnokov, 1997.8.6)
        hide Sequence Ontology: Class of Gene
        protein_coding_gene
         
        nuclear_gene
         
        hide Interactions & Pathways
        hide Summary of Genetic Interactions
        Interacts with
        Please look at the allele data for full details of the genetic interactions
        Orc1 allele
        Gene
        References
        hide External Data
        Linkouts
        BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
        •
        hide Orthologs
        Genome-wide drosophilid orthologs
        Curated drosophilid orthologs
         
        Linkouts
        InParanoid orthologs - Eukaryotic orthologs
        •
        hide Functional Complementation between Species
        hide Inter-Species Misexpression Data
        Produces phenotype in
        Produces NO phenotype in
        hide Stocks & Reagents
        hide Stocks Listed in FlyBase ( 2 )
        VDRC
        v46521
        w1118; P{GD4477}v46521
        v46522
        w1118; P{GD4477}v46522
        hide Genomic Clones ( 2 )
        hide cDNA Clones ( 17 )
        Please Note
        This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
        cDNA Clones, Fully Sequenced
        BDGP DGC clones
        Other clones
        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        hide RNAi & Array Information
        Affy Oligo
        Linkouts
        DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
        •
        GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
        •
        hide Antibody Information
        polyclonal
        (Chesnokov et al., 1999)
        hide Other Information
        hide Discoverer
        hide Etymology
        hide Identification
        hide Position Effect Variegation Data
        hide Relationship to Other Genes
        Source for database identity of
        Source for identity of: Orc1 CG10667
        (Gabrielian, 1999.11)
        Source for database merge of
        Additional comments
        hide Comments About Role
        Orc1 is required for ATP hydrolysis and ATP-dependent DNA binding.
        (Chesnokov et al., 2001)
        hide Comments About Molecular Function
        Transcription of Orc1 is coupled to cell cycle progression via direct regulation by E2f. The level of Orc1 protein appears to limit origin utilisation in at least two different cell types.
        (Asano and Wharton, 1999)
        Orc1 is degraded by the APC (anaphase-promoting complex).
        (Araki et al., 2003)
        The ATP dependent DNA binding activity of the Orc1/Orc2 protein complex is reduced or inhibited by phosphorylation of Orc1.
        (Remus et al., 2005)
        hide Other Comments
        hide External Crossreferences & Linkouts
        Sequence Crossreferences
        RefSeq (Transcripts)
        •
        RefSeq (Proteins)
        •
        DDBJ / EMBL / GenBank
        •
        UniProtKB/Swiss-Prot
        •
        Other Crossreferences
        InterPro domains - A database of protein families, domains, and functional sites
        •
        Bromo adjacent region (IPR001025)
        AAA+ ATPase, core (IPR003593)
        AAA ATPase, core (IPR003959)
        CDC6, C terminal (IPR015163)
        Linkouts
        BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
        •
        DEDB - Drosophila exon database: splicing graphs
        •
        DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
        •
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        •
        FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
        •
        InParanoid orthologs - Eukaryotic orthologs
        •
        Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
        •
        PANTHER - Protein classification by function, families, and pathways
        •
        hide Synonyms & Secondary IDs ( 10 )
        Reported As
        Symbol Synonym
        CG10667
         
        DmORC1
        (Bosco et al., 2001, Chesnokov,