A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Gene Dmel\Taf8

General Information
SymbolDmel\Taf8SpeciesD. melanogaster
NameTBP-associated factor 8Annotation symbolCG7128
Feature typeprotein_coding_geneFlyBase IDFBgn0022724
Created / Updated2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)XRecombination map
Cytogenetic map16F7-16F7Sequence locationX:17,996,005..17,997,517 [+]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene TBP-associated factor 8 is referred to in FlyBase by the symbol Taf8 (CG7128, FBgn0022724). It has the cytological map location 16F7. Its sequence location is X:17996005..17997517. Its molecular function is described as: RNA polymerase II transcription factor activity; protein binding. It is involved in the biological processes: transcription initiation from RNA polymerase II promoter; cellular process. 12 alleles are reported. The phenotype of these alleles is annotated with third instar larva. It has one annotated transcript and one annotated polypeptide.

hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
16F7-16F7  
Limits computationally determined from genome sequence between P{EP}EP970 and P{EP}ari-1EP317  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
16E-17A
 
(Ortuno-Sahagun, 1997.11.14)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
In direction of increasing cytology: l(1)16Fo? l(1)16Fp? l(1)16Fq? mnb? l(1)16Fa? l(1)16Fb? l(1)16Fc? Sh? anon-16Fc? l(1)16Fh? anon-16Fd? scu? anon-16Fe? Taf8? anon-16Ff? wupA? l(1)16Fe? l(1)16Fm? l(1)16Fk? l(1)16Fn? ari-1? Frq1?
(Prado et al., 1999)
Gene Order (overall orientation not stated)
References
hide Gene Model & Products
Please see the GBrowse view of Dmel\Taf8 for information on other features
detailed view FBtr0074527 FBtr0074514 FBtr0074515 FBpp0074288 FBpp0074289 FBti0037305 FBti0059239 FBti0049952 FBti0041817 FBti0059356 FBti0038330 FBti0070924
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
Taf8-RA
FBtr0074514
 NM_078673
  1341
  328
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
Taf8-PA  
FBpp0074288  
36.7  
328  
5.27  
  NP_523397
  AAF48800
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
•
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
Bromodomain transcription factor (IPR006565)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AA696483
 
AE003507
AAF48800
 
AY071604
AAL49226
 
BF492787
 
BF499649
 
BG633694
 
BI228588
 
BI235301
 
BI375994
 
BI482629
 
CO293804
 
CO303073
 
CO307805
 
CO315787
 
CO331039
 
EC057725
 
EC065605
 
EC067584
 
EC238112
 
EC238409
 
GM07760
 
Y15513
CAA75667
 
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
    Maps to
    Does NOT map to
    Identified with
    (BDGP Project Members, 2000-)
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    hide External Data
    Linkouts
    DEDB - Drosophila exon database: splicing graphs
    •
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
      Marker for
        Subcellular Localization
        CV Term
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Lethality
        Allele
        viable
        lethal
        lethal | larval stage
        cell lethal | somatic clone
        Other Phenotypes
        Allele
        wild-type
        Sterility
        Allele
        fertile
        Phenotype manifest in
        Allele
        third instar larva
        hide Classical Alleles ( 7 )
        For All Classical Alleles Show

        Allele of Taf8ClassMutagenStocksKnown lesion
        Taf822700 --
        Taf888-20 Yes
        Taf888-60 Yes
        Taf8880 Yes
        Taf8EY167671 --
        Taf8G00880 --
        Taf8unspecified
          		0 --
          hide Alleles Carried on Transgenic Constructs ( 5 )
          For All Alleles Carried on Transgenic Constructs Show

          Allele of Taf8ClassMutagenStocksKnown lesion
          Taf81-91.T:Scer\GAL40 Yes
          Taf8E6L0 Yes
          Taf8GD121412 Yes
          Taf8T:Ivir\HA10 Yes
          Taf8T:Scer\GAL40 Yes
          hide Aneuploid Aberrations
          Useful deficiency
           
          Disrupted in
          (Hernandez-Hernandez and Ferrus, 2001)
          Duplicated in
          (de la Pompa, 1994)
          hide Transgenic Constructs & Insertions
          Transgenic Constructs
          Type of construct
          Name
          Expression data
          UAS construct
          P{GD12141}
          NA
          characterization construct
          P{wupAE6L}
          NA
          Insertions
          Type of insertions
          Name
          Expression data
          miscellaneous insertions
          P{}Taf888
          NA
          insertion of enhancer trap
          P{lacW}Taf8G0088
          No
          insertion of mobile activating element
          P{EPgy2}Taf8EY16767
          NA
          hide Related Comments
          Please look at the allele reports for the complete phenotype data
          hide Gene Ontology: Function, Process & Cellular Component ( 5 )
          hide Molecular Function
          CV term
          Evidence
          References
          RNA polymerase II transcription factor activity
          inferred from direct assay
          (Hernandez-Hernandez and Ferrus, 2001)
          protein binding
          inferred from physical interaction with FLYBASE:Taf10b; FB:FBgn0026324
          (Hernandez-Hernandez and Ferrus, 2001)
          hide Biological Process
          CV term
          Evidence
          References
          cellular process
          inferred from mutant phenotype
          (Hernandez-Hernandez and Ferrus, 2001)
          transcription initiation from RNA polymerase II promoter
          inferred from direct assay
          (Hernandez-Hernandez and Ferrus, 2001)
          hide Cellular Component
          CV term
          Evidence
          References
          transcription factor TFIID complex
          inferred from direct assay
          (Hernandez-Hernandez and Ferrus, 2001)
          hide Sequence Ontology: Class of Gene
          protein_coding_gene
           
          nuclear_gene
           
          hide Interactions & Pathways
          hide Summary of Genetic Interactions
          Interacts with
          Please look at the allele data for full details of the genetic interactions
          Taf8 allele
          Gene
          References
          Taf82270
          Ras85D
          (Hernandez-Hernandez and Ferrus, 2001)
          Taf888-2
          Ras85D
          (Hernandez-Hernandez and Ferrus, 2001)
          Taf888-6
          Ras85D
          (Hernandez-Hernandez and Ferrus, 2001)
          Taf888
          Ras85D
          (Hernandez-Hernandez and Ferrus, 2001)
          Taf8E6L
          Ras85D
          (Hernandez-Hernandez and Ferrus, 2001)
          hide External Data
          Linkouts
          BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
          •
          hide Orthologs
          Genome-wide drosophilid orthologs
          Curated drosophilid orthologs
           
          Linkouts
          InParanoid orthologs - Eukaryotic orthologs
          •
          hide Functional Complementation between Species
          hide Inter-Species Misexpression Data
          Produces phenotype in
          Produces NO phenotype in
          hide Stocks & Reagents
          hide Stocks Listed in FlyBase ( 3 )
          VDRC
          v27870
          w1118; P{GD12141}v27870
          v27868
          w1118; P{GD12141}v27868/TM3
          Bloomington
          21235
          y1 w67c23 P{EPgy2}Taf8EY16767
          hide Genomic Clones ( 1 )
          hide cDNA Clones ( 12 )
          Please Note
          This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
          cDNA Clones, Fully Sequenced
          BDGP DGC clones
          Other clones
          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
          hide RNAi & Array Information
          Affy Oligo
          Linkouts
          DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
          •
          GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
          •
          hide Antibody Information
          hide Other Information
          hide Discoverer
          hide Etymology
          hide Identification
          hide Position Effect Variegation Data
          hide Relationship to Other Genes
          Source for database identity of
          Source for database merge of
          Source for merge of: prodos l(1)16Fg
          (Ferrus, 1999.3.4)
          Additional comments
          hide Comments About Role
          Taf8 is required for cell viability.
          (Hernandez-Hernandez and Ferrus, 2001)
          hide Comments About Molecular Function
          hide Other Comments
          hide External Crossreferences & Linkouts
          Sequence Crossreferences
          RefSeq (Transcripts)
          •
          RefSeq (Proteins)
          •
          DDBJ / EMBL / GenBank
          •
          UniProtKB/TrEMBL
          •
          Other Crossreferences
          InterPro domains - A database of protein families, domains, and functional sites
          •
          Bromodomain transcription factor (IPR006565)
          Linkouts
          BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
          •
          DEDB - Drosophila exon database: splicing graphs
          •
          DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
          •
          FLIGHT - Cell culture data for RNAi and other high-throughput technologies
          •
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          •
          FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans
          •
          GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
          •
          GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
          •
          InParanoid orthologs - Eukaryotic orthologs
          •
          PANTHER - Protein classification by function, families, and pathways
          •
          hide Synonyms & Secondary IDs ( 16 )
          Reported As
          Symbol Synonym
          CG7128
          (Aoyagi and Wassarman, 2000, )
          dmTAF8
          (Lebedeva, 2005, Lebedeva et al., 2005)
          HL-VIII
          (Ferrus, 1999.3.4, Prado et al., 1999)
          l(1)16Fg
           
          pds
          (Peter et al., 2002, Hernandez-Hernandez and Ferrus, 2001, de la Pompa, 1994, Ferrus et al., 1990, )
          pro
          (Prado et al., 1999)
          prod
          (Ortuno-Sahagun, 1997.11.14, )
          Prodos
          (Lebedeva, 2005, Lebedeva et al., 2005)
          TAF
          (Mueller and Tora, 2004)
          TAF8
          (Tora, 2002)
          Taf8
           
          TAFII
          (Arnosti, 2002)
          TFIID
          (Butler and Kadonaga, 2002, Hochheimer et al., 2002, Gu et al., 2002, Stathopoulos and Levine, 2002)
          Name Synonym
          prodos
          (Hernandez-Hernandez and Ferrus, 2001, Ortuno-Sahagun, 1997.11.14, de la Pompa, 1994, Ferrus et al., 1990, )
          TBP-associated factor
          (Schuettengruber, 2006)
          TBP-associated factor 8
           
          Secondary FlyBase IDs
          • FBgn0014200
          hide References ( 22 )
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          hide Recent research papers (0)
          All research papers listed in FlyBase were published before 2006
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          hide Recent reviews ( 1 )
          Schuettengruber, 2006, Cell 128(4): 735--745
          Genome regulation by polycomb and trithorax proteins. [FBrf0198664]
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