Gene Dmel\Thor

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General Information

Symbol

Dmel\Thor

Species

D. melanogaster

Name

Thor

Annotation symbol

CG8846

Feature type

protein_coding_gene

FlyBase ID

FBgn0022073

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

23F3-23F3

Sequence location

2L:3,478,434..3,479,621 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol Thor (CG8846, FBgn0022073). It has the cytological map location 23F3. Its sequence location is 2L:3478434..3479621. Its molecular function is described as eukaryotic initiation factor 4E binding. It is involved in the biological processes: immune response; defense response; regulation of cell growth; negative regulation of cell size; response to stress; triacylglycerol metabolic process; response to oxidative stress; response to starvation; antibacterial humoral response; negative regulation of translational initiation. 21 alleles are reported. The phenotypes of these alleles are annotated with: wing; dorsal mesothoracic disc; larval fat body; 1st posterior cell. It has one annotated transcript and one annotated polypeptide.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

23F3-23F3

Limits computationally determined from genome sequence between P{EP}Thor^EP818&P{lacW}Thor^k07736 and P{PZ}odd^rF111&P{PZ}for⁰⁶⁸⁶⁰

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

23F5-23F6

(determined by in situ hybridisation)

(Spradling et al., 1999)

23F-24A

(determined by in situ hybridisation)

(Rodriguez et al., 1996)

23F5-23F6

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Thor for information on other features

Comments on Gene Model

EST data suggest additional 5' exon(s)

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Thor-RA

FBtr0077524

NM_057947

764

117

Additional Transcript Data & Comments

Reported size (kB)

0.85 (northern blot)

(Bernal and Kimbrell, 2000)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Thor-PA

FBpp0077213

12.9

117

10.19

NP_477295

AAF51100

Additional Polypeptide Data & Comments

Reported size (kD)

117 (aa)

(Bernal and Kimbrell, 2000)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0022073

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Eukaryotic translation initiation factor 4E binding (IPR008606)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9XZ56

Maps to

FBcl0297459

(FlyBase, 1992-)

Does NOT map to

Identified with

HL08053

(Spradling et al., 1999, BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

protein binding site

Thor-protein_bind-1
2L:3,478,137..3,478,146

comment=bound_moiety is NF-kB
evidence=predicted

(Bernal and Kimbrell, 2000)

protein binding site

Thor-protein_bind-2
2L:3,478,149..3,478,161

comment=bound_moiety is NF-kB
evidence=predicted

(Bernal and Kimbrell, 2000)

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

5411

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

adult stage,oogenesis stage

ovary

(Bernal and Kimbrell, 2000)

embryonic stage

embryonic central nervous system

(Bernal and Kimbrell, 2000)

embryonic stage-adult stage

(Bernal and Kimbrell, 2000)

adult stage,oogenesis stage

nurse cell

(Bernal and Kimbrell, 2000)

larval stage | third instar

testis

(Bernal and Kimbrell, 2000)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AA699260

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0022073

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0022073

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0022073

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for Thor

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

Other Phenotypes

Allele

wild-type

Thor^1rv1

visible, with Scer\GAL4^Bx-MS1096

Thor^LL.Scer\UAS

visible, with Scer\GAL4^dpp.blk1

Thor^LL.Scer\UAS

reduced cell number | somatic clone, with Scer\GAL4^Act5C.PP

Thor^LL.Scer\UAS

reduced cell number, with Scer\GAL4^Bx-MS1096

Thor^LL.Scer\UAS

reduced cell size | somatic clone, with Scer\GAL4^Act5C.PP

Thor^LL.Scer\UAS

short lived

Thor^Δ1034-2617

short lived, with Scer\GAL4^hs.PB

Thor^{Y54A.M59A.Scer\UAS}

environmental stress response defective

Thor^Δ1034-2617

cell size defective, with Scer\GAL4^Act5C.PP

Thor^LL.Scer\UAS

immune response defective

Sterility

Allele

fertile

Phenotype manifest in

Allele

wing, with Scer\GAL4^Bx-MS1096

Thor^LL.Scer\UAS

dorsal mesothoracic disc, with Scer\GAL4^Bx-MS1096

Thor^LL.Scer\UAS

larval fat body, with Scer\GAL4^Act5C.PP

Thor^LL.Scer\UAS

dorsal mesothoracic disc | somatic clone, with Scer\GAL4^Act5C.PP

Thor^LL.Scer\UAS

1st posterior cell, with Scer\GAL4^dpp.blk1

Thor^LL.Scer\UAS

Classical Alleles ( 12 )

For All Classical Alleles Show

Allele of Thor	Class	Mutagen	Stocks	Known lesion
Thor^1rv1		P-element activity	0	--
Thor²	loss of function	P-element activity	1	Yes
Thor^DG19607		P-element activity	1	--
Thor^EP2020		P-element activity	1	--
Thor^EP2350		P-element activity	0	Yes
Thor^EP818		P-element activity	1	Yes
Thor^Rv1		P-element activity	0	Yes
Thor^k07730		P-element activity	0	--
Thor^k07736		P-element activity	1	--
Thor^k07739		P-element activity	0	--
Thor^k13517	hypomorph	P-element activity	1	Yes
Thor^Δ1034-2617		P-element activity	0	Yes

Alleles Carried on Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Thor	Mutagen	Stocks	Known lesion
Thor^AALL.Act5C	in vitro construct \| regulatory fusion	0	Yes
Thor^GD12533	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
Thor^LL.Act5C	in vitro construct \| regulatory fusion	0	Yes
Thor^LL.Scer\UAS	in vitro construct \| regulatory fusion in vitro construct \| amino acid replacement	1	Yes
Thor^Scer\UAS.cMa	in vitro construct \| regulatory fusion	2	Yes
Thor^Scer\UAS.cTa	in vitro construct \| regulatory fusion	0	Yes
Thor^Scer\UAS.cTb	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes
Thor^{T37A.T46A.Scer\UAS}	in vitro construct \| regulatory fusion in vitro construct \| site directed mutagenesis	0	Yes
Thor^{Y54A.M59A.Scer\UAS}	in vitro construct \| regulatory fusion in vitro construct \| site directed mutagenesis	0	Yes

Aneuploid Aberrations

Not disrupted in

Df(2L)JS17

(BDGP Project Members, 1994-1999)

Df(2L)S2590

(BDGP Project Members, 1994-1999)

In(2LR)DTD16^LDTD42^R

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Disrupted in

Df(2L)Exel7017

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD12533}

P{UAS-Thor-HA}

P{UAS-Thor.LL}

P{UAS-Thor.T37A.T46A}

P{UAS-Thor.T}

P{UAS-Thor.Y54A.M59A}

P{UAS-Thor.wt}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{?'lacW}Thor²

P{SUPor-P}KG08448

P{UAST-YFP.Rab6.T26N}01

P{UAST-YFP.RabX4.T40N}03

P{wHy}Thor^DG19607

insertion of enhancer trap

P{PZ}l(2)06270⁰⁶²⁷⁰

Yes

Yes

P{lacW}l(2)SH0490^SH0490

insertion of mobile activating element

Related Comments

Please look at the allele reports for the complete phenotype data

Cg25C is a member of the infection inducible enhancer detector strains.

(Rodriguez et al., 1996)

Gene Ontology: Function, Process & Cellular Component ( 11 )

Molecular Function

CV term

Evidence

References

eukaryotic initiation factor 4E binding

inferred from sequence or structural similarity

(Bernal and Kimbrell, 1998)

inferred from sequence or structural similarity

(Spradling et al., 1999)

inferred from sequence or structural similarity with HGNC_gene:EIF4EBP1; HGNC:3288; OMIM:602223

(Bernal and Kimbrell, 2000)

non-traceable author statement

(Bernal, 2000.3.10)

inferred from direct assay

(Miron et al., 2001)

Biological Process

CV term

Evidence

References

antibacterial humoral response

inferred from mutant phenotype

(Bernal and Kimbrell, 2000)

defense response

non-traceable author statement

(Lemaitre, 2000.12.20)

immune response

inferred from expression pattern AND inferred from mutant phenotype

(Bernal and Kimbrell, 2000)

non-traceable author statement

(Bernal, 2000.3.10)

negative regulation of cell size

traceable author statement

(Jacinto and Hall, 2003)

negative regulation of translational initiation

inferred from electronic annotation with InterPro:IPR008606

(FlyBase Curators et al., 2004-)

regulation of cell growth

inferred from genetic interaction with FLYBASE:Akt1; FB:FBgn0010379 AND inferred from genetic interaction with FLYBASE:Pi3K92E; FB:FBgn0015279 AND inferred from mutant phenotype

(Miron et al., 2001)

response to oxidative stress

inferred from mutant phenotype

(Tettweiler et al., 2005)

response to starvation

inferred from mutant phenotype

(Tettweiler et al., 2005)

response to stress

inferred from expression pattern

(Junger et al., 2003)

triacylglycerol metabolic process

inferred from mutant phenotype