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Gene Dmel\Dmn

General Information
Symbol	Dmel\Dmn	Species	D. melanogaster
Name	Dynamitin	Annotation symbol	CG8269
Feature type	protein_coding_gene	FlyBase ID	FBgn0021825
Created / Updated	2003-12-02/2003-12-02
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	44F3-44F3	Sequence location	2R:4,779,345..4,780,844 [+]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Dynamitin is referred to in FlyBase by the symbol Dmn (CG8269, FBgn0021825). It has the cytological map location 44F3. Its sequence location is 2R:4779345..4780844. Its molecular function is unknown. It is involved in the biological processes: microtubule-based movement; mitotic spindle organization and biogenesis; spindle organization and biogenesis; centrosome localization. 11 alleles are reported. The phenotypes of these alleles are annotated with: oocyte; dorsal appendage; larval nervous system. It has one annotated transcript and one annotated polypeptide.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 44F3-44F3   Limits computationally determined from genome sequence between P{lacW}Rya-r44Fk04913 and P{lacW}Dmnk16109&P{lacW}Gγ1k08017
Experimentally Determined Cytological Location
Cytogenetic map Notes References 44F3-44F4 (determined by in situ hybridisation)   (Spradling et al., 1999) 44F2-44F6 44F3--4   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\Dmn for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Dmn-RA FBtr0088641  NM_079951   1350   380
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Dmn-PA   FBpp0087722   42.0   380   4.91     NP_524690   AAF59034
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0021825
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Dynamitin (IPR006996)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA264300   AA696511   AA802366   AA802868   AA816547   AA817125   AE003835 AAF59034   AI239224   AQ026100   AW941674   AY061092 AAL28640   BF490629   BF504646   BG633635   BG633804   BI165146   BI173536   BI228913   BI237261   BI353963   BI355859   BI355872   CK131915   CO156387   CO270302   CO336633   CZ466455   CZ466456   EC064959   EC082809   EC263984   EC266706   EC267824   GM04270
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7K2D2
Maps to
Does NOT map to
Identified with	LD07994 (Spradling et al., 1999, BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 13301
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0021825 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0021825 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0021825 FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Dmn Stage(s) 1-3 Stage(s) 4-6 Stage(s) 7-8 Stage(s) 9-10 Stage(s) 11-12 Stage(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable DmnT:Avic\GFP lethal | recessive Dmnk16109 Dmnk16218 Sterility Allele fertile DmnT:Avic\GFP Phenotype manifest in Allele oocyte & nucleus, with Scer\GAL4mat.αTub67C.T:Hsim\VP16 DmnScer\UAS.T:Avic\GFP larval nervous system, with Scer\GAL4elav-C155 DmnScer\UAS.cDa stage S9 oocyte & nucleus Dmnhs.PD dorsal appendage Dmnhs.PD oocyte | germ-line clone Dmnk16109
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of Dmn Class Mutagen Stocks Known lesion Dmnc00434 piggyBac transposase 1 -- Dmnk16109 P-element activity 2 -- Dmnk16218 P-element activity 1 --
Alleles Carried on Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Dmn Class Mutagen Stocks Known lesion DmnGD13758 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes DmnScer\UAS.T:Avic\GFP in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes DmnScer\UAS.cDa in vitro construct | regulatory fusion 1 Yes DmnT:Avic\GFP in vitro construct | coding region fusion 0 Yes DmncWa in vitro construct | other 0 Yes DmndsRNA.cMa in vitro construct | RNAi 0 Yes Dmnhs.PD in vitro construct | regulatory fusion 0 Yes DmnαTub67C.T:Avic\GFP in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(2R)VV4R
Disrupted in	Df(2R)BSC269 (Christensen and Cook, 2007.3.22, Christensen and Cook, 2007.3.22) Df(2R)Exel8048 (Ryder, 2004.4.26) Df(2R)Np3 (Spradling et al., 1999, Januschke et al., 2002, BDGP Project Members, 1994-1999)
Not disrupted in	Df(2R)Np1 (Spradling et al., 1999, BDGP Project Members, 1994-1999) Df(2R)Np4 (Spradling et al., 1999, BDGP Project Members, 1994-1999) Df(2R)Np5 (Spradling et al., 1999, BDGP Project Members, 1994-1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD13758} NA P{UAS-Dmn.D} NA P{UAS-Dmn::GFP} NA characterization construct P{Dmn-GFP.W} NA P{tub-Dmn.GFP} NA heat-shock construct P{hs-Dmn.D} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{PB}Dmnc00434 NA insertion of enhancer trap P{lacW}Dmnk16109 No P{lacW}Dmnk16218 No
Related Comments
	Please look at the allele reports for the complete phenotype data RNAi screen using dsRNA made from templates generated with primers directed against this gene causes spindle pole detachment when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007)
Gene Ontology: Function, Process & Cellular Component ( 6 )
Molecular Function
CV term Evidence References
Biological Process
CV term Evidence References
centrosome localization inferred from mutant phenotype (Morales-Mulia and Scholey, 2005)
microtubule-based movement inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000)
mitotic spindle organization and biogenesis inferred from mutant phenotype (Goshima et al., 2007)
spindle organization and biogenesis inferred from mutant phenotype (Morales-Mulia and Scholey, 2005)
Cellular Component
CV term Evidence References
dynactin complex inferred from sequence or structural similarity (Spradling et al., 1999) inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) inferred from electronic annotation with InterPro:IPR006996 (FlyBase Curators et al., 2004-)
colocalizes_with mitochondrion inferred from direct assay (Pilling et al., 2006)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Dmn allele Gene References Dmnk16109 peb (Wilk et al., 2004)
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 68822
Orthologs
Genome-wide drosophilid orthologs	Dana\GF12385 Dere\GG23390 Dgri\GH23102 Dmoj\GI18927 Dper\GL17128 Dpse\GA20943 Dsec\GM21071 Dsim\GD10607 Dvir\GJ21298 Dwil\GK20679 Dyak\GE19232
Curated drosophilid orthologs	Dpse\GA20943
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000017909 Anopheles gambiae (Ensembl) ENSBTAP00000014053 Bos taurus (Ensembl) ENSCAFP00000000335 Canis familiaris (Ensembl) NEWSINFRUP00000144963 Takifugu rubripes (Ensembl) ENSP00000346785 Homo sapiens (Ensembl) ENSMMUP00000014805 Macaca mulatta (Ensembl) ENSPTRP00000040891 Pan troglodytes (Ensembl) ENSRNOP00000008120 Rattus norvegicus (Ensembl) GSTENP00013576001 Tetraodon nigroviridis (Ensembl) ENSXETP00000013692 Xenopus tropicalis (Ensembl) WBGene00030715 Caenorhabditis briggsae (Wormbase) WBGene00001018 Caenorhabditis elegans (Wormbase) ZDB-CDNA-040425-2213 Danio rerio (Zfin) ZDB-GENE-040426-1279 Danio rerio (Zfin) DDB0220488 Dictyostelium discoideum (Dictybase) MGI:107733 Mus musculus (Informatics)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 7 )
Harvard	- PBac{PB}Dmnc00434
VDRC	v23726 w1118; P{GD13758}v23726 v23728 w1118; P{GD13758}v23728
Bloomington	11159 y1 w67c23; P{lacW}Dmnk16109/CyO 8784 w*; P{UAS-Dmn.D}2; TM3, Sb1/TM6B, Tb1
Kyoto	102986 Df(1)w67c23, y1 ; P{lacW}Dmnk16109 / CyO 103002 Df(1)w67c23, y1 ; P{lacW}Dmnk16218 / CyO
Genomic Clones ( 1 )
	BACR02C23
cDNA Clones ( 21 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD07994
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT06245 GM32672 GM06479 GM25730 GM07813 AT27162 LD22263 AT29733 RE04968 LD02048 RE33453 LP12988 RE26573 GM32619 GM09053 RE16472
Other clones	EK098306 EN07951 GM04270 EN12931
RNAi & Array Information
Affy Oligo
Linkouts	DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0021825 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0021825
Antibody Information
Other Information
Discoverer
Etymology
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Dmn CG8269 (Misra, 2000.8.9)
Additional comments
Comments About Role
Comments About Molecular Function
Other Comments
	dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079951 RefSeq (Proteins) • NP_524690 DDBJ / EMBL / GenBank • AA264300 AE003835 AAF59034 AQ026100 AW941674 AY061092 AAL28640 CZ466455 CZ466456 UniProtKB/TrEMBL • Q7K2D2
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Dynamitin (IPR006996)
Linkouts
	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 68822 DEDB - Drosophila exon database: splicing graphs • 13301 DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0021825 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0021825 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0021825 FlyExpress - Embryonic expression images (BDGP data) • FBgn0021825 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0021825 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0021825 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0021825 InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000017909 Anopheles gambiae (Ensembl) ENSBTAP00000014053 Bos taurus (Ensembl) ENSCAFP00000000335 Canis familiaris (Ensembl) NEWSINFRUP00000144963 Takifugu rubripes (Ensembl) ENSP00000346785 Homo sapiens (Ensembl) ENSMMUP00000014805 Macaca mulatta (Ensembl) ENSPTRP00000040891 Pan troglodytes (Ensembl) ENSRNOP00000008120 Rattus norvegicus (Ensembl) GSTENP00013576001 Tetraodon nigroviridis (Ensembl) ENSXETP00000013692 Xenopus tropicalis (Ensembl) WBGene00030715 Caenorhabditis briggsae (Wormbase) WBGene00001018 Caenorhabditis elegans (Wormbase) ZDB-CDNA-040425-2213 Danio rerio (Zfin) ZDB-GENE-040426-1279 Danio rerio (Zfin) DDB0220488 Dictyostelium discoideum (Dictybase) MGI:107733 Mus musculus (Informatics) PANTHER - Protein classification by function, families, and pathways • FBgn0021825
Synonyms & Secondary IDs ( 14 )
Reported As
Symbol Synonym	CG8269 (Goldstein and Gunawardena, 2000, Goshima et al., 2007, Morales-Mulia and Scholey, 2005) dmn (Ji et al., 2002, Mirouse et al., 2006) Dmn (Christensen and Cook, 2007.3.22, Goshima et al., 2007) l(2)k16109 (BDGP Project Members, 1994-1999, ) l(2)k16218 (Dockendorff et al., 2000) P50 (Basto et al., 2004, Basto et al., 2004) p50/Dmn (Wojcik et al., 2001) p50/dynamitin (Tekotte and Davis, 2002, Welte, 2004)
Name Synonym	Dynactin (Papoulas et al., 2003) dynamitin (Huynh and St. Johnston, 2004, Duncan et al., 2001) Dynamitin (Spradling et al., 1999) p50/Dynamitin (Pilling et al., 2006) p50 dynamitin (Cytrynbaum et al., 2003) p50-dynamitin (Wojcik et al., 2000, Morales-Mulia and Scholey, 2005, Kim et al., 2007)
Secondary FlyBase IDs
	FBgn0033338
References ( 53 )
Generate a list of	All references relating to this gene ( 53 )
List References by type	Research paper  ( 20 ) Supplementary material  ( 6 ) Review  ( 5 ) Personal communication to FlyBase  ( 6 ) Abstract  ( 9 ) FlyBase analysis  ( 1 ) Computer file  ( 4 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 3 )
	Kim et al., 2007, J. Cell Biol. 176(5): 641--651 Microtubule binding by dynactin is required for microtubule organization but not cargo transport. [FBrf0193704] Mirouse et al., 2006, Development 133(20): 4005--4013 Interaction between Polo and BicD proteins links oocyte determination and meiosis control in Drosophila. [FBrf0192446] Pilling et al., 2006, Molec. Biol. Cell 17(4): 2057--2068 Kinesin-1 and Dynein are the primary motors for fast transport of mitochondria in Drosophila motor axons. [FBrf0191163] Show all research papers ...
Recent reviews (0)