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Gene Dmel\mira

General Information
Symbol	Dmel\mira	Species	D. melanogaster
Name	miranda	Annotation symbol	CG12249
Feature type	protein_coding_gene	FlyBase ID	FBgn0021776
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	3R	Recombination map	3-67.7
Cytogenetic map		Sequence location	3R:15,757,521..15,760,842 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene miranda is referred to in FlyBase by the symbol mira (CG12249, FBgn0021776). Its sequence location is 3R:15757521..15760842. Its molecular function is described as: actin binding; myosin binding. It is involved in the biological processes: asymmetric protein localization; asymmetric protein localization during cell fate commitment; ganglion mother cell fate determination; intracellular mRNA localization; neuroblast fate determination; determination of anterior/posterior axis, embryo; regulation of pole plasm oskar mRNA localization; asymmetric neuroblast division; neuroblast proliferation. 26 alleles are reported. The phenotypes of these alleles are annotated with: longitudinal connective; commissure; cell body glial cell; embryonic neuron; neuroblast NB6-4 of thorax; embryonic glial cell; embryonic abdomen; embryonic head; embryonic thorax; pole cell. It has 2 annotated transcripts and 2 annotated polypeptides.
External Summaries
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location
Experimentally Determined Cytological Location
Cytogenetic map Notes References 92C-92C   (Shen, 1998.2.2) 92B-92C   (Ikeshima-Kataoka, 1997.7.10) 92C-92C (determined by in situ hybridisation)   (Ikeshima-Kataoka et al., 1997) 92C-92C (determined by in situ hybridisation)   (Shen et al., 1997)
Experimentally Determined Recombination Data
Location	3-67.7 (Ikeshima-Kataoka et al., 1997)
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References In direction of increasing cytology: Hs6st- mira- CG4783+ Hs6st- MED25+ (Kamimura et al., 2001) Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\mira for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) mira-RA FBtr0083847  NM_057943   3322   829 mira-RB FBtr0083848  NM_057944   3232   799
Additional Transcript Data & Comments
Reported size (kB)	3.5, 3.4 (northern blot) (Shen et al., 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein mira-PA   FBpp0083256   93.1   829   5.75     NP_477291   AAF55723 mira-PB   FBpp0083257   89.8   799   5.85     NP_477292   AAN13815
Additional Polypeptide Data & Comments
Reported size (kD)	830 (aa) (Ikeshima-Kataoka et al., 1997) 830, 800 (aa) (Shen et al., 1997)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Prefoldin (IPR009053)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202754   AB005661 BAA24111   AC007711   AF045771 AAC02621   AW942033   BI167034   BI167255   BI169074   BI227452   BI236249   BI236843   BI362884   BI363583   BI363705   BI366004   BI367366   BI371461   BI482139   BI564347   BI566574   BI568574   BI579581   BI579731   BI580571   BI605196   BI626294   BI629196   BT004903 AAO47881   CG12249   CO264169   CO266452   CO282054   CO282613   CO289952   CO294514   CO298034   CO308070   CO308365   CO322229   CO328435   CO331585   CO339419   EC077656   EC085122   EC085969   EC201158   EC207759   EC209705   EC210406   EC210944   EC234009   EC235815   EC238149   EC254213   EC254579
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	O44224 O45116 Q8IN63 Q9VDR7
Maps to	FBcl0298105 (FlyBase, 1992-)
Does NOT map to
Identified with	LD02989 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References deletion mira[ZZ176] 3R:15,759,055..15,759,057 comment=A 169bp deletion causes a frameshift, followed by a stop codon evidence=experimental (Ikeshima-Kataoka et al., 1997) deletion mira[RR127] 3R:15,758,212..15,758,214 comment=A 10bp deletion induces a frameshift that replaces the C-terminal 103 amino acids with an unrelated stretch of 112 amino acids evidence=experimental (Ikeshima-Kataoka et al., 1997) insertion site mira[L44] 3R:15,760,084..15,760,086 comment=A 2 bp insertion gives rise to 33 unrelated amino acids after the first 103 amino acids evidence=experimental (Matsuzaki et al., 1998) point mutation mira[YY227] 3R:15,759,525..15,759,525 comment=Premature stop codon gives rise to a 290aa protein; site of nucleic acid substitution inferred by FlyBase based on reported amino acid change evidence=experimental na_change=C15759525T pr_change=Q291|mira-PA,Q291|mira-PB (Matsuzaki et al., 1998) point mutation mira[JJ92] 3R:15,759,408..15,759,408 comment=Premature stop codon gives rise to a 329aa protein; site of nucleic acid substitution inferred by FlyBase based on reported amino acid change evidence=experimental na_change=C15759408T pr_change=Q330|mira-PA,Q330|mira-PB (Matsuzaki et al., 1998) point mutation mira[AB78] 3R:15,759,180..15,759,180 comment=Premature stop codon gives rise to a 405aa protein; site of nucleic acid substitution inferred by FlyBase based on reported amino acid change evidence=experimental na_change=C15759180T pr_change=Q406|mira-PA,Q406|mira-PB (Matsuzaki et al., 1998)
External Data
Linkouts
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  embryonic stage | stage >=8  neuroblast (Shen et al., 1997)  embryonic stage | stage 3   (Shen et al., 1997)  embryonic stage | stage >=12  ventral nerve cord | ventral (Shen et al., 1997)  embryonic stage | stage >=8  sensillum precursor (Shen et al., 1997)  embryonic stage | stage >=8  posterior midgut primordium (Shen et al., 1997)  embryonic stage | stage >=12  embryonic brain (Shen et al., 1997)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference  embryonic stage | stage >=8  sensillum precursor (Ikeshima-Kataoka et al., 1997)  embryonic stage | stage >=8  midgut primordium (Ikeshima-Kataoka et al., 1997)  embryonic stage | stage >=8  posterior midgut primordium (Shen et al., 1997)  embryonic stage | embryonic cycle 1..9  whole organism (Mollinari et al., 2002)  embryonic stage | stage >=8  neuroblast (Ikeshima-Kataoka et al., 1997)  embryonic stage | stage >=12  embryonic brain (Shen et al., 1997)  larval stage | third instar  larval ventral ganglion & neuroblast (Mollinari et al., 2002)  embryonic stage  neuroblast (Petritsch et al., 2003)  embryonic stage-adult stage   (Mollinari et al., 2002)  embryonic stage | stage >=12  ventral nerve cord | ventral (Shen et al., 1997)  embryonic stage | early   (Ikeshima-Kataoka et al., 1997)  embryonic stage | stage >=8  sensillum precursor (Shen et al., 1997)  larval stage | third instar  larval ventral ganglion & ganglion mother cell (Mollinari et al., 2002)  embryonic stage | embryonic cycle 10..11  whole organism (Mollinari et al., 2002)  embryonic stage  neuroblast (Petritsch et al., 2003)  embryonic stage  neuroblast (Petritsch et al., 2003)  embryonic stage | stage >=8  peripheral nervous system (Shen et al., 1997)  embryonic stage | embryonic cycle 10..11  whole organism (Mollinari et al., 2002)  embryonic stage | embryonic cycle 10..11  whole organism (Mollinari et al., 2002)  larval stage | third instar  larval ventral ganglion & neuroblast (Mollinari et al., 2002)  embryonic stage | stage >=8  neuroblast (Shen et al., 1997)
Marker for
Subcellular Localization
CV Term	plasma membrane | basal (Ikeshima-Kataoka et al., 1997) plasma membrane | basal plasma membrane | apical (Shen et al., 1998) plasma membrane | apical plasma membrane | basal (Matsuzaki et al., 1998) plasma membrane | apical plasma membrane | basal centrosome (Schuldt et al., 1998)
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0021776 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0021776 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0021776 BDGP expression data • Show all BDGP original images, annotations and embryonic microarray data for mira FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for mira Stage(s) 4-6 Stage(s) 7-8 Stage(s) 9-10 Stage(s) 11-12 Stage(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | recessive miraDEB miraL44 miraRR127 miraZZ176 Other Phenotypes Allele visible | homeotic miraΔ103.αTub67C.T:Avic\GFP-m6 miraαTub67C.T:Avic\GFP-m6 tumorigenic miraZZ176 Phenotype manifest in Allele neuroblast NB6-4 of thorax miraL44 miraRR127 embryonic neuron miraL44 miraZZ176 longitudinal connective miraL44 miraRR127 miraZZ176 commissure miraL44 miraRR127 miraZZ176 embryonic glial cell miraRR127 cell body glial cell miraZZ176 embryonic abdomen | ectopic miraαTub67C.T:Avic\GFP-m6 miraΔ103.αTub67C.T:Avic\GFP-m6 embryonic head miraαTub67C.T:Avic\GFP-m6 miraΔ103.αTub67C.T:Avic\GFP-m6 embryonic thorax miraαTub67C.T:Avic\GFP-m6 miraΔ103.αTub67C.T:Avic\GFP-m6 pole cell | ectopic miraαTub67C.T:Avic\GFP-m6 miraΔ103.αTub67C.T:Avic\GFP-m6
Classical Alleles ( 9 )
For All Classical Alleles Show
Allele of mira Class Mutagen Stocks Known lesion miraAB78 ethyl methanesulfonate 0 Yes miraDEB diepoxybutane 0 -- miraJJ92 ethyl methanesulfonate 0 Yes miraL44 loss of function ethyl methanesulfonate 0 Yes miraRR127 hypomorph ethyl methanesulfonate 0 Yes miraYY227 ethyl methanesulfonate 0 Yes miraZZ176 loss of function ethyl methanesulfonate 0 Yes miraunspecified 0 -- mirazz178 0 --
Alleles Carried on Transgenic Constructs ( 17 )
For All Alleles Carried on Transgenic Constructs Show
Allele of mira Class Mutagen Stocks Known lesion mira126.hs.T:Zzzz\TAP in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes mira298.hs.T:Zzzz\TAP in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes mira300-830.hs.T:Zzzz\TAP in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes mira431.hs.T:Zzzz\TAP in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes miraC300-830.Scer\UAS.T:Hsap\MYC in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes miraGD1596 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes miraN114-298.Scer\UAS.T:Hsap\MYC in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes miraN298.Scer\UAS.T:Hsap\MYC in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes miraN431.Scer\UAS.T:Hsap\MYC in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes miraScer\UAS.T:Hsap\MYC in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes miraScer\UAS.T:Zzzz\FLAG in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes miraScer\UAS.cIa in vitro construct | regulatory fusion 0 -- miraScer\UAS.cSa in vitro construct | regulatory fusion 0 Yes miraT:Avic\GFP-S65T.I167T in vitro construct | coding region fusion 0 -- miradsRNA.cBa in vitro construct | RNAi 0 Yes miraΔ103.αTub67C.T:Avic\GFP-m6 in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes miraαTub67C.T:Avic\GFP-m6 in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(3R)I9
Disrupted in	Df(3R)I9 (Shen et al., 1998, Schuldt et al., 1998, Ikeshima-Kataoka et al., 1997)
Not disrupted in	Df(3R)K17 (Shen et al., 1998)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1596} NA P{UAS-miranda.C300-830.T:Myc} NA P{UAS-miranda.I} NA P{UAS-miranda.N114-298.T:Myc} NA P{UAS-miranda.N298.T:Myc} NA P{UAS-miranda.N431.T:Myc} NA P{UAS-miranda.S} NA P{UAS-miranda.T:Myc} NA characterization construct P{UAS-MirN-Gβ13F} NA P{tub-mira.GFP} NA P{tub-mira.Δ103.GFP} NA heat-shock construct P{hs-mira.126.TAP} NA P{hs-mira.298.TAP} NA P{hs-mira.300-830.TAP} NA P{hs-mira.431.TAP} NA
Insertions
	Type of insertions Name Expression data
Related Comments
	Please look at the allele reports for the complete phenotype data Loss of mira gene function abolishes asymmetric localisation of pros protein during mitosis. (Shen et al., 1997)
Gene Ontology: Function, Process & Cellular Component ( 18 )
Molecular Function
CV term Evidence References
actin binding inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000)
myosin binding inferred from physical interaction with FLYBASE:jar; FB:FBgn0011225 (Petritsch et al., 2003)
Biological Process
CV term Evidence References
asymmetric neuroblast division traceable author statement (Skeath and Thor, 2003)
asymmetric protein localization inferred from genetic interaction with FLYBASE:pros; FB:FBgn0004595 AND inferred from mutant phenotype AND inferred from physical interaction with FLYBASE:pros; FB:FBgn0004595 (Ikeshima-Kataoka et al., 1997)
asymmetric protein localization during cell fate commitment traceable author statement (Schaefer and Knoblich, 2001) traceable author statement (Bardin et al., 2004)
determination of anterior/posterior axis, embryo inferred from mutant phenotype (Irion et al., 2006)
ganglion mother cell fate determination traceable author statement (Schaefer and Knoblich, 2001)
intracellular mRNA localization non-traceable author statement (Tuxworth and Chia, 2003)
neuroblast fate determination traceable author statement (Bardin et al., 2004)
neuroblast proliferation inferred from mutant phenotype (Caussinus and Gonzalez, 2005)
regulation of pole plasm oskar mRNA localization inferred from mutant phenotype (Irion et al., 2006)
Cellular Component
CV term Evidence References
apical cortex inferred from direct assay (Petritsch et al., 2003)
apical part of cell traceable author statement (Ohshiro et al., 2000) traceable author statement (Peng et al., 2000)
colocalizes_with aster inferred from direct assay (Petritsch et al., 2003)
basal cortex non-traceable author statement (Hong et al., 2001) traceable author statement (Ohshiro et al., 2000) traceable author statement (Peng et al., 2000) non-traceable author statement (Cayouette and Raff, 2002)
basal part of cell traceable author statement (Gonczy, 2002) inferred from direct assay (Petritsch et al., 2003)
cell cortex inferred from direct assay (Akiyama-Oda et al., 2000) inferred from direct assay (Petritsch et al., 2003)
cytoplasm traceable author statement (Ohshiro et al., 2000) inferred from direct assay (Petritsch et al., 2003)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions mira allele Gene References miraL44 pros (Akiyama-Oda et al., 2000) unspecified swa (Irion et al., 2006) exu (Irion et al., 2006) stau (Irion et al., 2006)
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 67367
Orthologs
Genome-wide drosophilid orthologs	Dpse\GA11506 Dsec\GM26878
Curated drosophilid orthologs	Dpse\GA11506   Dsim\mira (Begun, 2000.4.5) Dyak\mira (Begun, 2000.4.7)
Linkouts
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents