Gene Dmel\Nrk

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General Information

Symbol

Dmel\Nrk

Species

D. melanogaster

Name

Neurospecific receptor kinase

Annotation symbol

CG4007

Feature type

protein_coding_gene

FlyBase ID

FBgn0020391

Created / Updated

2003-12-02/2003-12-02

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

49F4-49F4

Sequence location

2R:9,047,243..9,050,334 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Neurospecific receptor kinase is referred to in FlyBase by the symbol Nrk (CG4007, FBgn0020391). It has the cytological map location 49F4. Its sequence location is 2R:9047243..9050334. Its molecular function is described as: protein-tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding. It is involved in the biological processes: protein amino acid phosphorylation; signal transduction. 4 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

49F4-49F4

Limits computationally determined from genome sequence between P{lacW}Psc^k07834&P{lacW}Su(z)2^k06344 and P{EP}EP873^EP873

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

49F-49F

(determined by in situ hybridisation)

(Oishi et al., 1997)

49F7-49F8

(determined by in situ hybridisation)

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Nrk for information on other features

Comments on Gene Model

Shares UTR: 5' UTR shared with upstream gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Nrk-RA

FBtr0087728

NM_057907

2905

724

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Nrk-PA

FBpp0086841

81.8

724

6.62

NP_477255

AAF58420

Additional Polypeptide Data & Comments

Reported size (kD)

714 (aa)

(Oishi et al., 1997)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0020391

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Kringle (IPR000001)

Frizzled cysteine-rich domain (IPR000024)

Protein kinase, core (IPR000719)

Tyrosine protein kinase (IPR001245)

Tyrosine protein kinase, active site (IPR008266)

Protein kinase-like (IPR011009)

Kringle-like fold (IPR013806)

Tyrosine-protein kinase transmembrane receptor ROR (IPR016247)

Protein kinase ATP binding, conserved site (IPR017441)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9V6K3

UniProtKB/TrEMBL

Maps to

DS01087

(FlyBase, 1992-)

Does NOT map to

Identified with

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

12692

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

embryonic stage-adult stage

(Oishi et al., 1997)

embryonic stage | stage >=10

neurogenic region

(Oishi et al., 1997)

embryonic stage | stage >=10

embryonic central nervous system

(Oishi et al., 1997)

embryonic stage | stage 17

peripheral nervous system

(Oishi et al., 1997)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0020391

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0020391

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0020391

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | recessive

Nrk^k14301

Phenotype manifest in

Allele

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of Nrk	Class	Mutagen	Stocks	Known lesion
Nrk^k14301		P-element activity	1	Yes

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Nrk	Mutagen	Stocks	Known lesion
Nrk^GD14403	in vitro construct \| RNAi in vitro construct \| regulatory fusion	3	Yes
Nrk^GD2540	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
Nrk^GD44	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes

Aneuploid Aberrations

Useful deficiency

Df(2R)vg-B

Useful duplication

Dp(2;2)M14

Disrupted in

Df(2R)Exel8057

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD14403}

P{GD2540}

P{GD44}

Insertions

Type of insertions

Name

Expression data

insertion of enhancer trap

P{lacW}Nrk^k14301

Related Comments

Please look at the allele reports for the complete phenotype data

When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, a whole range of mitotic abnormalities, spindle abnormalities, chromosome abnormalities are chromosome alignment defects are seen.

(Bettencourt-Dias et al., 2004)

Gene Ontology: Function, Process & Cellular Component ( 6 )

Molecular Function

CV term

Evidence

References

ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR001245, InterPro:IPR008266, InterPro:IPR017441

(FlyBase Curators et al., 2004-)

protein tyrosine kinase activity

inferred from direct assay

(Oishi et al., 1997)

non-traceable author statement

(Minami, 1997.2.25)

non-traceable author statement

(Oates, 1997.11.10)

non-traceable author statement

(Morrison et al., 2000)

transmembrane receptor protein tyrosine kinase activity

inferred from sequence or structural similarity

(Oishi et al., 1997)

inferred from sequence or structural similarity with EMBL:AF006464

(FlyBase, 1992-)

Biological Process

CV term

Evidence

References

protein amino acid phosphorylation

inferred from direct assay

(Oishi et al., 1997)

non-traceable author statement

(Morrison et al., 2000)

signal transduction

inferred from direct assay

(Oishi et al., 1997)

Cellular Component

CV term

Evidence

References

plasma membrane

inferred from sequence or structural similarity

(Oishi et al., 1997)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Nrk allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

62212

Orthologs

Genome-wide drosophilid orthologs

Dana\GF12938

Dere\GG20358

Dgri\GH20314

Dmoj\GI19986

Dper\GL11237

Dpse\GA17849

Dsec\GM21445

Dsim\GD10943

Dvir\GJ21235

Dwil\GK21680

Dyak\GE12517

Curated drosophilid orthologs

Dpse\GA17849

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000007679
Anopheles gambiae (Ensembl)
ENSBTAP00000003558
Bos taurus (Ensembl)
ENSCAFP00000004415
Canis familiaris (Ensembl)
NEWSINFRUP00000152947
Takifugu rubripes (Ensembl)
ENSGALP00000032134
Gallus gallus (Ensembl)
ENSP00000189978
Homo sapiens (Ensembl)
ENSMMUP00000009260
Macaca mulatta (Ensembl)
ENSMODP00000004898
Monodelphis domestica (Ensembl)
ENSPTRP00000036297
Pan troglodytes (Ensembl)
ENSRNOP00000043038
Rattus norvegicus (Ensembl)
GSTENP00003647001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000005563
Xenopus tropicalis (Ensembl)
ZDB-GENE-030131-6458
Danio rerio (Zfin)
MGI:103581
Mus musculus (Informatics)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 6 )

VDRC

v9653

w¹¹¹⁸; P{GD2540}v9653

v841

w¹¹¹⁸; P{GD44}v841/TM3

v36282

w¹¹¹⁸; P{GD14403}v36282

v36284

w¹¹¹⁸; P{GD14403}v36284

v42442

w¹¹¹⁸; P{GD14403}v42442

Bloomington

11122

y¹ w^67c23; P{lacW}Nrk^k14301 CG34439^k14301 TppII^k14301/CyO

Genomic Clones ( 1 )

BACR48I10

cDNA Clones ( 18 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0020391

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0020391

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Identified by PCR fragment; relationship to other protein tyrosine kinase genes not known.

(Oates, 1997.11.10)

Comments About Role

Comments About Molecular Function

Other Comments

Identification and characterisation of a new member of the Trk/Ror family RTKs, Nrk, which is expressed specifically in the developing nervous system during embryogenesis.

(Oishi et al., 1997)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•