Gene Dmel\pan

General Information
Symbol	Dmel\pan	Species	D. melanogaster
Name	pangolin	Annotation symbol	CG34403
Feature type	protein_coding_gene	FlyBase ID	FBgn0085432
Created / Updated	2005-09-09/2007-07-06
Genomic Location
Chromosome (arm)	4	Recombination map
Cytogenetic map	102A4-102A4 102A3-102A4	Sequence location	4:89,956..129,430 [+]
Map ( GBrowse )
Summary Information
Automatically generated summary See sections below for more information	The gene pangolin is referred to in FlyBase by the symbol pan (CG34403, FBgn0085432). It has the cytological map location 102A4. Its sequence location is 4:89956..129430. Its molecular function is described as: specific transcriptional repressor activity; DNA binding. It is involved in the biological processes described with 11 unique terms, many of which group under: anatomical structure development; regulation of biological process; organ development; embryonic pattern specification; regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway; cell communication; regulation of signal transduction; regulation of metabolic process; signal transduction; transcription, DNA-dependent; mesoderm morphogenesis; regulation of developmental process; regionalization; open tracheal system development; organ morphogenesis. 41 alleles are reported. The phenotypes of these alleles are annotated with 45 unique terms, many of which group under: organ system; adult segment; cuticle; embryonic/larval tracheal system; integumentary system; muscle founder cell; peripheral nervous system; late extended germ band embryo; adult mesothoracic segment; thoracic segment. It has 10 annotated transcripts and 10 annotated polypeptides.
External Summaries

Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 102A4-102A4 Limits computationally determined from genome sequence between P{SUPor-P}KG01127&PBac{5HPw⁺}A173 and PBac{5HPw⁺}A437
Experimentally Determined Cytological Location
Cytogenetic map Notes References 101F-101F (determined by in situ hybridisation) (van de Wetering et al., 1997)
Experimentally Determined Recombination Data
Location	4- (Brunner et al., 1997, Kronhamn and Rasmuson-Lestander, 1997)
Left of (cM)
Right of (cM)
Notes	Maps close to ci. (Kronhamn and Rasmuson-Lestander, 1997)
Molecular Map Data
Gene Order (in direction of increasing cytology) References In direction of increasing cytology: ci- RpS3A- pan+ (van Beest et al., 1998, Kronhamn and Rasmuson-Lestander, 1999) Gene Order (overall orientation not stated) References Overall orientation not stated: ci- pan+ (Brunner et al., 1997, van de Wetering et al., 1997, Dooijes et al., 1998)
Gene Model & Products
Please see the GBrowse view of Dmel\pan for information on other features
Comments on Gene Model
	Gene merge based on EST/cDNA data Transposon inserted in intron EST data support existence of multiple transcripts due to variable use of 5' exons that are non-coding
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) pan-RA FBtr0089161 NM_166718 2799 751 pan-RB FBtr0089159 NM_079880 2988 751 pan-RC FBtr0089160 NM_166719 3274 751 pan-RD FBtr0089162 NM_166720 2839 751 pan-RE FBtr0089156 NM_166721 2868 751 pan-RF FBtr0089158 NM_166722 2742 751 pan-RG FBtr0089157 NM_166723 2800 751 pan-RH FBtr0100245 NM_001014685 2976 747 pan-RI FBtr0100246 NM_001038709 3519 410 pan-RJ FBtr0112657 NM_166724 3891 1192
Additional Transcript Data & Comments
Reported size (kB)	2.8 (compiled cDNA) (Brunner et al., 1997)
Comments	The major (A) and minor (B) forms of pan transcript differ in the second part of the HMG box. (van de Wetering et al., 1997, van de Wetering et al., 1997)
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein pan-PA FBpp0088228 81.9 751 7.20 NP_726522 AAF59371 pan-PB FBpp0088226 81.9 751 7.20 NP_524619 AAN06544 pan-PC FBpp0088227 81.9 751 7.20 NP_726523 AAF59370 pan-PD FBpp0088229 81.9 751 7.20 NP_726524 AAN06545 pan-PE FBpp0088223 81.9 751 7.20 NP_726525 AAN06546 pan-PF FBpp0088225 81.9 751 7.20 NP_726526 AAN06547 pan-PG FBpp0088224 81.9 751 7.20 NP_726527 AAN06548 pan-PH FBpp0099631 81.4 747 7.29 NP_001014685 AAX52517 pan-PI FBpp0099632 46.3 410 9.21 NP_001033798 ABC65830 pan-PJ FBpp0111569 132.1 1192 7.13 NP_726528 AAN06549
Additional Polypeptide Data & Comments
Reported size (kD)	751 (aa) (Brunner et al., 1997)
Comments	A fusion construct containing amino acids 1 to 130 of wild-type pan protein (pan^wt.cBa) binds 4-5 fold more of the vertebrate arm protein homolog beta-catenin in-vitro than fusion constructs containing amino acids 1 to 130 of either pan^S25 or pan^S28 protein. The pan^S25 and pan^S28 proteins each differ from wild type pan protein by one amino acid. (Anonymous, 1997) Repeats 3-8 of the arm protein interact with aa 1-90 of pan in a yeast two-hybrid assay. The DNA binding site of the pan HMG box was assayed, and revealed the consensus CCTTTGATCTT. Cotransfection of arm and pan vectors resulted in transcriptional activation of a Cat or a Ppyr\LUC reporter, and the C-terminus of arm protein was necessary for this activation. The transactivation capacity of the C-terminus of arm was further demonstrated by fusing it to a GAL4-DNA binding domain, and assaying expression of a CAT reporter. (van de Wetering et al., 1997)
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • CTNNB1 binding, N-teminal (IPR013558) High mobility group box, HMG (IPR009071) High mobility group box, HMG1/HMG2 (IPR000910)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA695697 AA698063 AE003845 AAF59370 AE003845 AAF59371 AE003845 AAN06544 AE003845 AAN06545 AE003845 AAN06546 AE003845 AAN06547 AE003845 AAN06548 AE003845 AAN06549 AW941213 AY075359 AY312729 AAQ67543 AY312730 AAQ67544 AY312731 AAQ67545 AY312732 AAQ67546 AY312733 AAQ67547 AY312734 AAQ67548 AY312735 AAQ67549 AY312736 AAQ67550 AY312737 AAQ67551 AY312738 AAQ67552 AY312739 AAQ67553 AY312740 AAQ67554 AY312741 AAQ67555 AY312742 AAQ67556 AY312743 AAQ67557 AY312744 AAQ67558 AY312745 AAQ67559 AY312746 AAQ67560 AY312747 AAQ67561 AY312748 AAQ67562 AY312749 AAQ67563 AY312750 AAQ67564 AY312751 AAQ67565 AY312752 AAQ67566 BI369695 BI372507 BI372521 BI592441 BT011132 BT021431 AAX33579 BT029282 BZ286237 CO177395 CO177630 CO186599 CO188140 CO190934 CO266779 CO272804 CO275315 CO277488 CO279037 CO284300 CO284369 CO286315 CO286633 CO295169 CO299620 CO310183 CO312604 CO313578 CO315017 CO323031 CO324787 CO325361 CO327415 CO327963 CO344605 CZ470551 CZ482871 CZ484725 CZ485006 EC058523 EC062241 EC064620 EC066828 EC074628 EC083214 EC084517 EC088134 EC195693 EC199344 EC200947 EC207118 EC207856 EC212212 EC215230 EC217889 EC218376 EC222206 EC222283 EC223815 EC232399 EC236970 EC240754 EC241378 EC244541 EC245712 EC253219 EC256583 EC257420 U88538 AAC47464 Y09125 CAA70343

UniProtKB/Swiss-Prot	P91943 Q8IMA8
UniProtKB/TrEMBL	A4V148 Q8IMA8

Maps to
Does NOT map to
Identified with	GH23203 GM04312 HL03718 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference embryonic stage \| contracted germ band procephalic segment (Brunner et al., 1997) embryonic stage \| early (Brunner et al., 1997) embryonic stage \| 0-2hr (van de Wetering et al., 1997) embryonic stage \| stage 7 (Schweizer and Basler, 1998) embryonic stage (van de Wetering et al., 1997) embryonic stage \| contracted germ band stage visceral mesoderm (Brunner et al., 1997)
Marker for

Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for

Subcellular Localization
CV Term
External Data & Images
Linkouts
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable pan^S25 pan^S28 lethal \| recessive pan¹³ pan^Δ31 lethal \| recessive \| conditional ts pan^ER1 lethal \| male, with Scer\GAL4^Bx-MS1096 pan^ΔN.Scer\UAS lethal \| embryonic stage \| rescuable maternal effect \| segment polarity pan² lethal \| embryonic stage \| recessive pan^13a pan³ pan^IA5 lethal \| embryonic stage \| segment polarity, with Scer\GAL4^e22c pan^ΔN.Scer\UAS lethal \| embryonic stage, with Scer\GAL4^e22c pan^ΔN.Scer\UAS lethal \| embryonic stage, with Scer\GAL4^unspecified pan^{Scer\UAS.T:Hsim\VP16} lethal \| embryonic stage, with Scer\GAL4^arm.PS pan^ΔN.Scer\UAS lethal \| larval stage \| recessive pan¹³ pan^13a lethal \| pupal stage, with Scer\GAL4^dpp.blk1 pan^ΔN.Scer\UAS lethal \| pharate adult stage, with Scer\GAL4^30A pan^ΔN.Scer\UAS lethal, with Scer\GAL4^hs.2sev pan^Scer\UAS.cWa lethal, with Scer\GAL4^elav-C155 pan^Scer\UAS.cWa lethal, with Scer\GAL4^bs-1348 pan^ΔN.Scer\UAS semi-lethal \| pupal stage, with Scer\GAL4^dpp.blk1 pan^Scer\UAS.cWa Other Phenotypes Allele wild-type pan^hs.PW wild-type, with Scer\GAL4^e22c pan^Scer\UAS.cWa visible pan^ER1 visible \| recessive pan^13a visible, with Scer\GAL4^T155 pan^ΔN.Scer\UAS visible, with Scer\GAL4^vg.int2.1 pan^Scer\UAS.cWa visible, with Scer\GAL4^sev.EP pan^Scer\UAS.cWa size defective \| somatic clone pan² mitotic, with Scer\GAL4^bbg-C96 pan^ΔN.Scer\UAS Minute \| dominant pan^Δ3M pan^Δ9M Phenotype manifest in Allele ovary \| somatic clone, with Scer\GAL4^Act5C.PP pan^ΔN.Scer\UAS dorsal mesothoracic disc \| somatic clone, with Scer\GAL4^Act5C.PP pan^ΔN.Scer\UAS dorsal mesothoracic disc, with Scer\GAL4^bbg-C96 pan^ΔN.Scer\UAS filzkorper, with Scer\GAL4^ems.HRE pan^ΔN.Scer\UAS stigmatophore, with Scer\GAL4^salm-459.2 pan^ΔN.Scer\UAS dorsal fine hair, with Scer\GAL4^ptc-559.1 pan^ΔN.Scer\UAS dorsal thick hair \| ectopic, with Scer\GAL4^ptc-559.1 pan^ΔN.Scer\UAS dorsal denticle \| ectopic, with Scer\GAL4^ptc-559.1 pan^ΔN.Scer\UAS parasegment, with Scer\GAL4^ptc-559.1 pan^ΔN.Scer\UAS embryonic epidermis, with Scer\GAL4^ptc-559.1 pan^ΔN.Scer\UAS denticle belt, with Scer\GAL4^ptc-559.1 pan^ΔN.Scer\UAS embryonic epidermis, with Scer\GAL4^e22c pan^ΔN.Scer\UAS denticle belt, with Scer\GAL4^e22c pan^ΔN.Scer\UAS pan^Scer\UAS.cWa segment, with Scer\GAL4^e22c pan^ΔN.Scer\UAS embryo \| segment polarity, with Scer\GAL4^e22c pan^ΔN.Scer\UAS embryonic/first instar larval cuticle, with Scer\GAL4^e22c pan^ΔN.Scer\UAS ventral thoracic disc, with Scer\GAL4^Dll-md23 pan^ΔN.Scer\UAS abdominal sternite, with Scer\GAL4^T155 pan^ΔN.Scer\UAS abdominal tergite, with Scer\GAL4^T155 pan^ΔN.Scer\UAS embryonic epidermis, with Scer\GAL4^unspecified