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Gene Dmel\sktl

General Information
Symbol	Dmel\sktl	Species	D. melanogaster
Name	skittles	Annotation symbol	CG9985
Feature type	protein_coding_gene	FlyBase ID	FBgn0016984
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	2R	Recombination map	2-
Cytogenetic map	57B3-57B3	Sequence location	2R:16,714,916..16,719,919 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene skittles is referred to in FlyBase by the symbol sktl (CG9985, FBgn0016984). It has the cytological map location 57B3. Its sequence location is 2R:16714916..16719919. Its molecular function is described by 1-phosphatidylinositol-4-phosphate 5-kinase activity. It is involved in the biological processes: phosphorylation; peripheral nervous system development; autophagic cell death; salivary gland cell autophagic cell death; phosphatidylinositol metabolic process. 22 alleles are reported. The phenotypes of these alleles are annotated with: embryonic peripheral nervous system; chordotonal organ; imaginal disc; macrochaeta; wing; scutum; ovary; dorsal appendage; egg. It has one annotated transcript and one annotated polypeptide.
External Summaries
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 57B3-57B3   Limits computationally determined from genome sequence between P{lacW}l(2)k06409k06409&P{PZ}insc05475 and P{PZ}l(2)0780607806
Experimentally Determined Cytological Location
Cytogenetic map Notes References 57B1-57B1   (Prokopenko, 1999.8.3) 57B-57B   (Prokopenko, 1998.6.10) 57B1-57B20   (Knirr, 1996.10.6) 57B2-57B3 (determined by in situ hybridisation)   (Spradling et al., 1999) 57B1-57B5 (determined by in situ hybridisation)   (Kania et al., 1995) 57B-57B (determined by in situ hybridisation) 57B1--3 (determined by in situ hybridisation) 57B2--3 (determined by in situ hybridisation)   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location	2- (Knirr, 1996.10.6)
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References Overall orientation not stated: insc+ sktl? insc+ (Breuer et al., 1997, Hassan et al., 1997) Overall orientation not stated: insc? sktl? insc? (Knirr et al., 1997) Overall orientation not stated: insc+ sktl+ insc+ (Knirr et al., 1997)
Gene Model & Products
Please see the GBrowse view of Dmel\sktl for information on other features
Comments on Gene Model
	EST data suggest additional 3' exon(s)
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) sktl-RA FBtr0071563  NM_057858   3614   792
Additional Transcript Data & Comments
Reported size (kB)	3.8 (northern blot) (Hassan et al., 1998) 4.1 (unknown) (Knirr et al., 1997) 4.1 (northern blot) (Knirr et al., 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein sktl-PA   FBpp0071491   87.8   792   7.33     NP_477206   AAF46627
Additional Polypeptide Data & Comments
Reported size (kD)	700 (aa) (Hassan et al., 1998)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0016984
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Phosphatidylinositol-4-phosphate 5-kinase, core (IPR002498) Phosphatidylinositol-4-phosphate 5-kinase, core, subgroup (IPR016034)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA141052   AA540159   AC004433   AE003452 AAF46627   AF071417 AAC25576   AF174679   AF174680   AI259817   AI293572   AW944020   AX093901   AY058737 AAL13966   BG636577   BG639681   BI170756   BI234552   BI240082   BI356677   BI365265   BI368421   BI369948   BI370189   BI371751   BI584816   BI632750   BI633778   BI638213   BI638826   BP548606   CK01121   CK658579   CO279866   CO308447   CO312127   EC083725   EC226594   EC248115   G00585   U73490 AAB18233
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7JNK2 Q94544 Q9W2R3
Maps to	DS03659 (FlyBase, 1992-)
Does NOT map to
Identified with	LP03320 (Spradling et al., 1999, BDGP Project Members, 2000-) LP06742 (Cheng and Shearn, 2004)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 1140
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  embryonic stage | stage 11-13  embryonic central nervous system | precursor (Hassan et al., 1998)  embryonic stage | stage 5  procephalic neurectoderm (Hassan et al., 1998)  embryonic stage | stage 17  embryonic central nervous system | restricted (Hassan et al., 1998)  spermatogenesis  spermatocyte (Knirr et al., 1997)  spermatogenesis   (Knirr et al., 1997)  embryonic stage | gastrulation  ventral furrow (Hassan et al., 1998)  larval stage | third instar  photoreceptor cell R8 | precursor (Hassan et al., 1998)  embryonic stage | stage 4-5  procephalic neurectoderm (Knirr et al., 1997)  embryonic stage | stage 13  embryonic/larval midgut (Hassan et al., 1998)  embryonic stage | stage 13  mesoderm (Hassan et al., 1998)  embryonic stage | stage 13  embryonic/larval hindgut (Hassan et al., 1998)  adult stage | female   (Knirr et al., 1997)  larval stage | third instar  morphogenetic furrow | anterior to (Hassan et al., 1998)  oogenesis stage  nurse cell (Knirr et al., 1997)  embryonic stage | 0-24 hr   (Knirr et al., 1997)  embryonic stage   (Knirr et al., 1997)  embryonic stage | stage 11-13  embryonic peripheral nervous system | precursor (Hassan et al., 1998)  embryonic stage | stage 12-13  ectoderm (Knirr et al., 1997)  embryonic stage | stage 17  embryonic/larval foregut | restricted (Hassan et al., 1998)  oogenesis stage  oocyte (Knirr et al., 1997)  embryonic stage | stage 17  embryonic/larval pharynx (Hassan et al., 1998)  embryonic stage | gastrulation  cephalic furrow (Hassan et al., 1998)  embryonic stage | stage 13  embryonic/larval dorsal vessel (Hassan et al., 1998)  embryonic stage | stage 17  embryonic/larval hindgut | restricted (Hassan et al., 1998)  embryonic stage  mesoderm (Knirr et al., 1997)  embryonic stage  embryonic nervous system (Knirr et al., 1997)  oogenesis stage  germarium | restricted (Knirr et al., 1997)  embryonic stage | stage 13  embryonic/larval foregut (Hassan et al., 1998)  larval stage | third instar  imaginal disc (Hassan et al., 1998)  embryonic stage | stage 17  embryonic/larval midgut | restricted (Hassan et al., 1998)  embryonic stage   (Hassan et al., 1998)  larval stage | third instar  larval brain (Hassan et al., 1998)  oogenesis stage   (Knirr et al., 1997)  embryonic stage | stage 11  pole cell (Hassan et al., 1998)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AI259817
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0016984 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0016984 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0016984 FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for sktl Stage(s) 1-3 Stage(s) 4-6 Stage(s) 7-8 Stage(s) 9-10 Stage(s) 11-12 Stage(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | recessive sktl03679 sktl05475 sktlk02517 sktlk10907 sktlk13706 sktlΔ13 lethal | embryonic stage | recessive sktlk07505 sktlk07701 sktlk12405 sktlΔ20 lethal | larval stage | recessive sktlΔ15 sktlΔ24 sktlΔ31 sktlΔ5 sktlΔ9 lethal | larval stage, with Scer\GAL4T80 sktlScer\UAS.cHa lethal | larval stage, with Scer\GAL4da.G32 sktlScer\UAS.cHa lethal | adult stage | recessive sktlk02608 semi-lethal sktl05475 Other Phenotypes Allele visible, with Scer\GAL4dpp.blk1 sktlScer\UAS.cHa Phenotype manifest in Allele ovary sktlΔ15 nurse cell & nucleus sktlΔ15 dorsal appendage sktlΔ15 egg sktlΔ15 wing sktlΔ15 sktlΔ5 wing & macrochaeta sktlΔ15 sktlΔ5 wing & macrochaeta, with Scer\GAL4dpp.blk1 sktlScer\UAS.cHa scutum & macrochaeta, with Scer\GAL4dpp.blk1 sktlScer\UAS.cHa macrochaeta | ectopic, with Scer\GAL4dpp.blk1 sktlScer\UAS.cHa wing, with Scer\GAL4dpp.blk1 sktlScer\UAS.cHa scutum, with Scer\GAL4dpp.blk1 sktlScer\UAS.cHa wing & macrochaeta, with Scer\GAL432B sktlScer\UAS.cHa scutum & macrochaeta, with Scer\GAL432B sktlScer\UAS.cHa macrochaeta | ectopic, with Scer\GAL432B sktlScer\UAS.cHa wing, with Scer\GAL432B sktlScer\UAS.cHa scutum, with Scer\GAL432B sktlScer\UAS.cHa macrochaeta, with Scer\GAL432B sktlScer\UAS.cHa imaginal disc sktlk02517 embryonic peripheral nervous system sktlk02608 sktlk07505 sktlk07701 sktlk12405 chordotonal organ | lateral sktlk02608 sktlk07505 sktlk07701 sktlk12405
Classical Alleles ( 19 )
For All Classical Alleles Show
Allele of sktl Class Mutagen Stocks Known lesion sktl03679 P-element activity 0 -- sktl05475 P-element activity 2 Yes sktlDG24311 1 -- sktlk02517 P-element activity 0 -- sktlk02608 P-element activity 0 Yes sktlk07505 P-element activity 0 Yes sktlk07701 P-element activity 0 Yes sktlk10907 P-element activity 0 -- sktlk12405 P-element activity 1 Yes sktlk13706 P-element activity 0 -- sktlunspecified 0 -- sktlΔ1-1 hypomorph 0 Yes sktlΔ13 Δ2-3 0 -- sktlΔ15 loss of function Δ2-3 0 Yes sktlΔ20 loss of function Δ2-3 0 Yes sktlΔ24 Δ2-3 0 Yes sktlΔ31 Δ2-3 0 Yes sktlΔ5 loss of function Δ2-3 0 Yes sktlΔ9 Δ2-3 0 Yes
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of sktl Class Mutagen Stocks Known lesion sktlGD1534 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes sktlScer\UAS.T:Zzzz\FLAG in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes sktlScer\UAS.cHa in vitro construct | regulatory fusion 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(2R)E2   Df(2R)Pu-D17
Useful duplication	Dp(2;Y)53D;57C
Disrupted in	Df(2R)Exel6070 (Ryder, 2004.4.26) Df(2R)P72 (Knirr et al., 1997) Df(2R)Pu-D17 (Spradling et al., 1999) Df(2R)inscP49 (Hassan et al., 1998, Knirr et al., 1997)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1534} NA P{UAS-sktl.FLAG} NA P{UAS-sktl.H} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions ?{}sktlΔ1-1 NA P{wHy}sktlDG24311 NA insertion of enhancer trap P{PZ}insc03679 No P{PZ}insc05475 Yes P{lacW}insck02517 No P{lacW}insck02608 No P{lacW}insck07505 Yes P{lacW}insck07701 Yes P{lacW}insck10907 No P{lacW}insck12405 Yes P{lacW}insck13706 No P{lacW}l(2)SH0488SH0488 No insertion of mobile activating element P{EP}inscEP2010 NA
Related Comments
	Please look at the allele reports for the complete phenotype data Mutants isolated in a screen of the second chromosome identifying gene affecting disc morphology. (Roch et al., 1998)
Gene Ontology: Function, Process & Cellular Component ( 7 )
Molecular Function
CV term Evidence References
1-phosphatidylinositol-4-phosphate 5-kinase activity inferred from sequence or structural similarity with NCBI_gi:4505815 (FlyBase, 1992-) non-traceable author statement (Morrison et al., 2000) traceable author statement (Prokopenko et al., 2000)
Biological Process
CV term Evidence References
autophagic cell death inferred from expression pattern (Gorski et al., 2003)
peripheral nervous system development inferred from mutant phenotype (Prokopenko et al., 2000)
phosphatidylinositol metabolic process inferred from electronic annotation with InterPro:IPR002498 (FlyBase Curators et al., 2004-)
phosphorylation non-traceable author statement (Morrison et al., 2000)
salivary gland cell autophagic cell death inferred from expression pattern (Gorski et al., 2003)
Cellular Component
CV term Evidence References
nucleus inferred from sequence or structural similarity (Prokopenko et al., 2000)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions sktl allele Gene References sktlk07505 ash1 (Cheng and Shearn, 2004) ash2 (Cheng and Shearn, 2004) sktlΔ15 ash1 (Cheng and Shearn, 2004) ash2 (Cheng and Shearn, 2004) sktlΔ20 ash1 (Cheng and Shearn, 2004) ash2 (Cheng and Shearn, 2004) unspecified rdgA (Garcia-Murillas et al., 2006)
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 63002
Orthologs
Genome-wide drosophilid orthologs	Dana\GF12741 Dere\GG20828 Dgri\GH19882 Dmoj\GI19831 Dpse\GA22167 Dsec\GM15773 Dsim\GD25249 Dvir\GJ18651 Dwil\GK22881 Dyak\GE13767
Curated drosophilid orthologs	Dpse\GA22167
Linkouts	InParanoid orthologs - Eukaryotic orthologs • Q8LN60 Oryza sativa (Gramene)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 12 )
Szeged	- P{EP}inscEP2010 - P{lacW}l(2)SH0488SH0488
VDRC	v6229 w1118; P{GD1534}v6229 v6231 w1118; P{GD1534}v6231/CyO
Kyoto	102229 Df(1)w67c23, y1 ; P{lacW}insck02517 / CyO 102234 Df(1)w67c23, y1 ; P{lacW}insck02608 / CyO 102772 Df(1)w67c23, y1 ; P{lacW}insck10907 / CyO 102892 Df(1)w67c23, y1 ; P{lacW}insck13706 / CyO 108869 cn1 P{PZ}insc05475 P{PZ}sktl05475/CyO; ry506
Bloomington	11409 cn1 P{PZ}insc05475 sktl05475/CyO; ry506 10373 y1 w67c23; P{lacW}insck12405 sktlk12405/CyO 21245 y1 w67c23; P{wHy}sktlDG24311 inscDG24311/SM6a
Genomic Clones ( 2 )
	BACR04E05 BACR10P16
cDNA Clones ( 24 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see