Gene Dmel\spen

General Information
Symbol	Dmel\spen	Species	D. melanogaster
Name	split ends	Annotation symbol	CG18497
Feature type	protein_coding_gene	FlyBase ID	FBgn0016977
Created / Updated	2005-09-09/2005-09-09
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	21B3-21B4	Sequence location	2L:159,034..203,251 [+]
Map ( GBrowse )
Summary Information
Automatically generated summary See sections below for more information	The gene split ends is referred to in FlyBase by the symbol spen (CG18497, FBgn0016977). It has the cytological map location 21B3-21B4. Its sequence location is 2L:159034..203251. Its molecular function is described as: transcription regulator activity; nucleic acid binding; nucleotide binding. It is involved in the biological processes described with 12 unique terms, many of which group under: anatomical structure development; cell motility; cell communication; organ morphogenesis; gliogenesis; neuroblast fate commitment; transmembrane receptor protein tyrosine kinase signaling pathway; Wnt receptor signaling pathway; segment specification; regulation of metabolic process. 82 alleles are reported. The phenotypes of these alleles are annotated with 40 unique terms, many of which group under: nervous system; organ system; peripheral nervous system; cephalopharyngeal skeleton; embryonic nervous system; adult segment; adult mesothoracic segment; embryonic head; pharyngeal sclerite; commissure. It has 3 annotated transcripts and 3 annotated polypeptides.
External Summaries

Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 21B3-21B4 Limits computationally determined from genome sequence between P{lacW}spen^k06805&P{lacW}l(2)k13604^k13604 and P{lacW}U2af38^k14504
Experimentally Determined Cytological Location
Cytogenetic map Notes References 21B4-21B6 (determined by in situ hybridisation) (Abdelilah-Seyfried et al., 2000) 21B4-21B6 (Rebay, 1999.9.8) 21A4-21B6 (Wiellette, 1999.9.18) 21B4-21B6 (determined by in situ hybridisation) (Spradling et al., 1999) 21B4-21B5 21B4--6 (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location	2- (Kolodziej et al., 1995, Lane et al., 2000) 2-1.0 +/- 0.5 (Dickson et al., 1996)
Left of (cM)	px (Kolodziej et al., 1995)
Right of (cM)	pr (Kolodziej et al., 1995)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\spen for information on other features
Comments on Gene Model
	Transposon fragment inserted in intron DGC clone LD15253 appears problematic: incomplete CDS DGC clone LD03167 appears problematic: incomplete CDS DGC clone SD03119 appears problematic: incomplete CDS DGC clone GM01870 appears problematic: genomic contaminant.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) spen-RA FBtr0078122 NM_164374 19535 5560 spen-RB FBtr0078121 NM_079979 19456 5533 spen-RC FBtr0078123 NM_164375 18809 5476
Additional Transcript Data & Comments
Reported size (kB)	20 (northern blot) (Wiellette et al., 1999)
Comments	Although the transcript specific to the shorter spen isoform is predicted to be 1 kb smaller than the one specific to the longer isoform, only 20 kb transcripts are detected on embryonic developmental northern blots. (Wiellette et al., 1999)
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein spen-PA FBpp0077781 600.0 5560 8.90 NP_722615 AAF51535 spen-PB FBpp0077780 597.2 5533 8.88 NP_524718 AAF51534 spen-PC FBpp0077782 590.5 5476 8.84 NP_722616 AAN10511
Additional Polypeptide Data & Comments
Reported size (kD)	5533, 5476 (aa); 597 (kD predicted) (Wiellette et al., 1999) 5476 (aa) (Rebay et al., 2000)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0016977
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • RNA recognition motif, RNP-1 (IPR000504) Spen paralogue and orthologue C-terminal, metazoa (IPR010912) Nucleotide-binding, alpha-beta plait (IPR012677) Spen paralogue and orthologue C-terminal (IPR012921) Spen Paralogue and Orthologue C-terminal-like (IPR016194)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202841 AA246553 AA440319 AA567849 AA950754 AA979070 AC005334 AE003590 AAF51534 AE003590 AAF51535 AE003590 AAN10511 AF184612 AAF26299 AF188205 AAF13218 AF195186 AF221715 AAF34661 AI135571 AI237923 AI296461 AI296533 AI531840 AQ025806 AQ025855 AQ254876 AW941153 AW942038 AW942345 AW944286 AY060834 AAL28382 AY075571 AY094788 AAM11141 AY863551 AY863552 AY863553 AY863554 AY863555 AY863556 AY863557 AY863558 AY863559 AY863560 AY863561 AY863562 AY863563 AY863564 AY863565 AY863566 AY863567 AY863568 AY863569 AY863570 AY863571 AY863572 AY863573 BF495889 BF503834 BG632612 BG635395 BH634796 BI238613 BI353182 BI361387 BI618312 BI625009 BI635338 BI635415 BI635705 BI640578 BP544795 BP544796 BP548792 BP548793 BP552278 BP555452 BP555453 BP559615 BQ103203 BT024966 CK605112 CL868578 CO153463 CO188981 CO189968 CO196478 CO267104 CO271856 CO273157 CO275657 CO276991 CO278591 CO286148 CO287017 CO288894 CO290302 CO292473 CO299209 CO300411 CO303093 CO303220 CO308622 CO313049 CO313465 CO318118 CO318164 CO319029 CO319052 CO319916 CO322692 CO324910 CO327553 CO334126 CO337459 CO337465 CO338568 CO341822 CO344273 CO344350 CZ472853 CZ472854 CZ473517 CZ477852 CZ478897 CZ480437 CZ481541 CZ482858 CZ483311 CZ485765 CZ485766 CZ489860 EC056741 EC056802 EC058436 EC059483 EC063231 EC065111 EC065253 EC065923 EC069414 EC070351 EC070850 EC074375 EC077012 EC079935 EC080673 EC084136 EC084636 EC085168 EC087967 EC194664 EC197723 EC201421 EC204525 EC206421 EC209314 EC209710 EC215949 EC216953 EC217251 EC221508 EC222157 EC222856 EC224105 EC224308 EC228405 EC230136 EC231312 EC231476 EC233442 EC235034 EC235038 EC235314 EC238337 EC239940 EC242096 EC244066 EC244070 EC244133 EC251974 EC252565 EC253381 EC255090 EC257600 EC263659 EC266998 EC268028 EL875363 EL878814 EL884088 EL884089 FF579074 FF579120 FF579341 FF579565 G00736

UniProtKB/Swiss-Prot	Q8SX83
UniProtKB/TrEMBL	Q95SE2

Maps to	DS08221 (Kuang et al., 2000)
Does NOT map to
Identified with	GM01870 LD03167 LD15253 SD03119 (BDGP Project Members, 2000-) Dm0625 (BDGP Project Members, 1994-1999, Spradling et al., 1999)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data