Gene Dmel\fz2

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General Information

Symbol

Dmel\fz2

Species

D. melanogaster

Name

frizzled 2

Annotation symbol

CG9739

Feature type

protein_coding_gene

FlyBase ID

FBgn0016797

Created / Updated

2003-12-01/2008-04-23

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

75F9-76A1

Sequence location

3L:19,135,998..19,228,191 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene frizzled 2 is referred to in FlyBase by the symbol fz2 (CG9739, FBgn0016797). It has the cytological map location 75F9-76A1. Its sequence location is 3L:19135998..19228191. Its molecular function is described as: Wnt receptor activity; Wnt-protein binding; protein binding; transmembrane receptor activity; non-G-protein coupled 7TM receptor activity; G-protein coupled receptor activity. It is involved in the biological processes: Wnt receptor signaling pathway; establishment and/or maintenance of cell polarity; Wnt receptor signaling pathway, calcium modulating pathway; cell motility; female gonad development; bristle morphogenesis; receptor-mediated endocytosis; signal transduction; axon extension. 85 alleles are reported. The phenotypes of these alleles are annotated with 19 unique terms, many of which group under: organ system; nervous system; peripheral nervous system; adult segment; adult mesothoracic segment; integumentary system; cuticle; cell component; morphogenetic furrow; t-bar; embryonic neuron; postsynaptic membrane; neuromuscular junction. It has 4 annotated transcripts and 4 annotated polypeptides.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

75F9-76A1

Limits computationally determined from genome sequence between P{EP}MESR6^EP3142 and P{lacW}l(3)L3809^L3809

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

76A-76A

(Bhanot, 1996.7.30)

Experimentally Determined Recombination Data

Location

(Bhanot, 1996.7.30)

Left of (cM)

(Chen and Struhl, 1999)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\fz2 for information on other features

Comments on Gene Model

Candidate for stop codon suppression.

3' UTR contains an ORF that is conserved among close Drosophila species (potential peptide 102 aa in length).

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

fz2-RA

FBtr0075029

NM_079431

3673

694

fz2-RB

FBtr0075028

NM_168787

3834

694

fz2-RC

FBtr0112890

NM_001104173

4443

694

fz2-RD

FBtr0299586

4298

694

Additional Transcript Data & Comments

Reported size (kB)

5.5 (northern blot)

(Bhanot et al., 1996)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

fz2-PA

FBpp0074796

75.5

694

8.25

NP_524155

AAF49184

fz2-PB

FBpp0074795

75.5

694

8.25

NP_730389

AAF49185

fz2-PC

FBpp0111803

75.5

694

8.25

NP_001097643

ABW08569

fz2-PD

FBpp0288861

75.5

694

8.25

Additional Polypeptide Data & Comments

Reported size (kD)

694 (aa)

(Bhanot et al., 1996)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0016797

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Frizzled cysteine-rich domain (IPR000024)

Frizzled protein (IPR000539)

GPCR, family 2, secretin-like (IPR000832)

Frizzled related (IPR015526)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9VVX3

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

LD10629

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

point mutation

fz2[C3]
3L:19,139,035..19,139,035

comment=Substitution of 4th conserved Cys by Ser; site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
evidence=experimental
na_change=T19139035A
pr_change=C118S|fz2-PB,C118S|fz2-PA,C118S|fz2-PC
reported_pr_change=C?S

(Chen et al., 2004)

point mutation

fz2[e1]
3L:19,138,585..19,138,585

comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
evidence=experimental
na_change=C19138585T
pr_change=Q268|fz2-PB,Q268|fz2-PA,Q268@|fz2-PC
reported_pr_change=Q268@

(Povelones et al., 2005)

point mutation

fz2[e2]
3L:19,138,532..19,138,533

comment=G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
evidence=experimental
reported_pr_change=W285

(Povelones et al., 2005, Povelones and Nusse, 2005)

point mutation

fz2[C1]
3L:19,138,428..19,138,428

comment=TGG to TAG mutation in codon W320
evidence=experimental
na_change=G19138428A
pr_change=W320|fz2-PB,W320|fz2-PA,W320@|fz2-PC
reported_na_change=G?A
reported_pr_change=W320@

(Chen and Struhl, 1999)

point mutation

fz2[e3]
3L:19,137,927..19,137,927

comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
evidence=experimental
na_change=C19137927T
pr_change=P487L|fz2-PB,P487L|fz2-PA,P487L|fz2-PC
reported_pr_change=P487L

(Povelones et al., 2005)

point mutation

fz2[C2]
3L:19,137,841..19,137,841

comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
evidence=experimental
na_change=C19137841T
pr_change=Q516|fz2-PB,Q516|fz2-PA,Q516@|fz2-PC
reported_pr_change=?516@

(Chen et al., 2004)

point mutation

fz2[e4]
3L:19,137,578..19,137,579

(Povelones et al., 2005)

point mutation

fz2[e5]
3L:19,137,578..19,137,579

(Povelones et al., 2005)

point mutation

fz2[C4]
3L:19,137,572..19,137,573

(Chen et al., 2004)

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

3590

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

embryonic stage | stage 10

procephalic neurogenic region

(Bhanot et al., 1996)

embryonic stage | stage 9-17

embryonic central nervous system | presumptive

(Bhanot et al., 1996)

embryonic stage | stage 12-16

visceral mesoderm

(Bhanot et al., 1996)

embryonic stage | stage 10

posterior midgut primordium

(Bhanot et al., 1996)

embryonic stage | stage 8

(Bhanot et al., 1996)

embryonic stage | stage 6

(Bhanot et al., 1996)

embryonic stage | stage 13-17

embryonic/larval hindgut

(Bhanot et al., 1996)

embryonic stage-adult stage

(Bhanot et al., 1996)

embryonic stage | stage 17

embryonic/larval dorsal vessel

(Bhanot et al., 1996)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

embryonic stage | stage 10

posterior midgut primordium

(Bhanot et al., 1996)

embryonic stage | stage 10

procephalic neurogenic region

(Bhanot et al., 1996)

embryonic stage | stage 12-16

visceral mesoderm

(Bhanot et al., 1996)

embryonic stage | stage 9-17

embryonic central nervous system | presumptive

(Bhanot et al., 1996)

embryonic stage | stage 17

embryonic/larval dorsal vessel

(Bhanot et al., 1996)

embryonic stage | stage 13-17

embryonic/larval hindgut

(Bhanot et al., 1996)

larval stage | third instar

bouton | subset

(Packard et al., 2002)

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0016797

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0016797

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0016797

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

fz2^C1

lethal | pupal stage | conditional ts, with Scer\GAL4^h-1J3

fz2^Scer\UAS.cCa

lethal, with Scer\GAL4^Dll-md23

fz2^{2-2-2.Scer\UAS.T:Hsap\MYC}

semi-lethal

fz2^CB02997

Other Phenotypes

Allele

wild-type, with Scer\GAL4^hs.2sev

fz2^{2-2-2.Scer\UAS.T:Hsap\MYC}

visible, with Scer\GAL4^69B

visible, with Scer\GAL4^71B

visible, with Scer\GAL4^Bx-MS1096

fz2^Scer\UAS.cCa

visible, with Scer\GAL4^h-1J3

fz2^Scer\UAS.cCa

visible, with Scer\GAL4^C-765

fz2^Scer\UAS.cSa

visible, with Scer\GAL4^bbg-C96

visible, with Scer\GAL4^unspecified

fz2^{ECD.GPI.Scer\UAS.T:Hsap\MYC}

small body

fz2^C1

cell death decrease | pupal stage, with Scer\GAL4^GMR.PF

fz2^{ECD.GPI.Scer\UAS.T:Hsap\MYC}

neuroanatomy defective

fz2^C1

neuroanatomy defective, with Scer\GAL4^C57

fz2^{C.Scer\UAS.NLS.T:Hsap\MYC}

tissue polarity, with Scer\GAL4^hs.2sev

fz2^Scer\UAS.N

Sterility

Allele

sterile

fz2^C1

Phenotype manifest in

Allele

wing margin bristle | ectopic, with Scer\GAL4^dpp.blk1

fz2^{2-2-2.Scer\UAS.T:Hsap\MYC}

ovary

fz2^C1

axon & dorsal cluster neuron

fz2^C1

NMJ bouton (with Df(3L)ED4782)

fz2^C1

RP2 neuron, with Scer\GAL4^da.G32

fz2^{ECD.GPI.Scer\UAS.T:Hsap\MYC}

embryonic epidermis | dorsal, with Scer\GAL4^da.G32

dorsal denticle, with Scer\GAL4^da.G32

RP2 neuron, with Scer\GAL4^hs.PB

fz2^{ECD.GPI.Scer\UAS.T:Hsap\MYC}

wing, with Scer\GAL4^unspecified

fz2^{ECD.GPI.Scer\UAS.T:Hsap\MYC}

eye photoreceptor cell & pupa, with Scer\GAL4^GMR.PF

fz2^{ECD.GPI.Scer\UAS.T:Hsap\MYC}

wing, with Scer\GAL4^bbg-C96

wing, with Scer\GAL4^69B

wing margin bristle, with Scer\GAL4^bbg-C96

fz2^Scer\UAS.N

wing, with Scer\GAL4^sd-SG29.1

fz2^Scer\UAS.N

wing margin, with Scer\GAL4^sd-SG29.1

fz2^Scer\UAS.N

eye, with Scer\GAL4^hs.2sev

fz2^Scer\UAS.N

ommatidium, with Scer\GAL4^hs.2sev

fz2^Scer\UAS.N

morphogenetic furrow | ectopic, with Scer\GAL4^dpp.blk1

fz2^Scer\UAS.N

wing margin bristle | ectopic, with Scer\GAL4^Bx-MS1096

wing, with Scer\GAL4^71B

wing margin bristle | ectopic, with Scer\GAL4^71B

wing & macrochaeta, with Scer\GAL4^69B

macrochaeta | ectopic, with Scer\GAL4^69B

wing sensillum | ectopic, with Scer\GAL4^69B

bouton, with Scer\GAL4^C57

neuromuscular junction, with Scer\GAL4^C57

postsynaptic membrane, with Scer\GAL4^C57

fz2^Scer\UAS.cCa

mitochondrion & bouton, with Scer\GAL4^C57

fz2^Scer\UAS.cCa

t-bar, with Scer\GAL4^C57

fz2^Scer\UAS.cCa

wing, with Scer\GAL4^Bx-MS1096

fz2^Scer\UAS.cCa

interfollicle cell | supernumerary | conditional ts, with Scer\GAL4^hs.PB

fz2^Scer\UAS.cCa

wing, with Scer\GAL4^h-1J3

fz2^Scer\UAS.cCa

wing sensillum | ectopic, with Scer\GAL4^h-1J3

fz2^Scer\UAS.cCa

wing sensillum | ectopic, with Scer\GAL4^71B

fz2^Scer\UAS.cCa

chaeta | ectopic & wing, with Scer\GAL4^bbg-C96

fz2^Scer\UAS.cCa

wing, with Scer\GAL4^C-765

fz2^Scer\UAS.cSa

wing margin bristle | ectopic, with Scer\GAL4^C-765

fz2^Scer\UAS.cSa

wing margin bristle | ectopic, with Scer\GAL4^unspecified

fz2^{Scer\UAS.cTa.T:Hsap\MYC}

denticle row 1, with Scer\GAL4^en-e16E

fz2^Scer\UAS.cZa

Classical Alleles ( 20 )

For All Classical Alleles Show

Allele of fz2	Class	Mutagen	Stocks	Known lesion
fz2⁴⁶⁹		P-element activity	0	Yes
fz2^C1	loss of function	ethyl methanesulfonate	0	Yes
fz2^{C2.Scer\UAS.T:wg,T:Ivir\HA1}			0	Yes
fz2^C2		ethyl methanesulfonate	0	Yes
fz2^{C3.Scer\UAS.T:wg,T:Ivir\HA1}			0	Yes
fz2^C3		ethyl methanesulfonate	0	Yes
fz2^C4		ethyl methanesulfonate	0	Yes
fz2^CB02997		P-element activity	0	Yes
fz2^CRD			0	--
fz2^M2-8			0	--
fz2^MB01455			1	--
fz2^e1		ethyl methanesulfonate	0	Yes
fz2^e2		ethyl methanesulfonate	0	Yes
fz2^e3		ethyl methanesulfonate	0	Yes
fz2^e4		ethyl methanesulfonate	0	Yes
fz2^e5		ethyl methanesulfonate	0	Yes
fz2^e6		ethyl methanesulfonate	0	Yes
fz2^f01018		piggyBac transposase	1	--
fz2^f07590		piggyBac transposase	1	--
fz2^unspecified			0	--

Alleles Carried on Transgenic Constructs ( 65 )

For All Alleles Carried on Transgenic Constructs Show